Heliothis virescens (Tobacco budworm moth)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A4JLX9|A0A2A4JLX9_HELVI Uncharacterized protein OS=Heliothis virescens OX=7102 GN=B5V51_853 PE=3 SV=1
LL1 pKa = 7.98SPIVALVPTTSSTTTTTKK19 pKa = 10.65KK20 pKa = 8.99PTTACPHH27 pKa = 6.21ATTKK31 pKa = 9.47PTVKK35 pKa = 8.14TTTQKK40 pKa = 9.4PTTTKK45 pKa = 10.28TSTTICSKK53 pKa = 7.51TTTRR57 pKa = 11.84KK58 pKa = 9.23PVATTPTEE66 pKa = 3.87PDD68 pKa = 2.58ITTTKK73 pKa = 9.98TYY75 pKa = 8.55HH76 pKa = 4.97TTPNVDD82 pKa = 2.37ITTPGLNTITPIEE95 pKa = 4.02PHH97 pKa = 5.33VTTPSGPDD105 pKa = 3.06VTTPTGPEE113 pKa = 3.75VTTPGGPEE121 pKa = 3.68VTTPGGPEE129 pKa = 3.68VTTPGGPEE137 pKa = 3.29ITTPGGPEE145 pKa = 3.27ITTPGGPEE153 pKa = 3.27ITTPGGPEE161 pKa = 3.68VTTPGGPEE169 pKa = 3.71LTTPSKK175 pKa = 11.16PEE177 pKa = 3.59VTTPSGPEE185 pKa = 3.24ITTPGGPEE193 pKa = 3.68VTTPGGPEE201 pKa = 3.71VTTTSGPEE209 pKa = 3.31ITTPGGPEE217 pKa = 3.69VTTPSGPEE225 pKa = 3.24ITTPGGPEE233 pKa = 3.27ITTPGGPEE241 pKa = 3.27ITTPGGPEE249 pKa = 3.68VTTPGGPEE257 pKa = 3.93LTTSSGPEE265 pKa = 3.54VTTPNGPEE273 pKa = 3.43ITTPGGPEE281 pKa = 3.69VTTPSGPEE289 pKa = 3.24ITTPGGPEE297 pKa = 3.72EE298 pKa = 4.34TTPGGPDD305 pKa = 3.08TTTAPCGHH313 pKa = 7.46DD314 pKa = 3.06EE315 pKa = 4.35TTPSEE320 pKa = 4.37TDD322 pKa = 3.23TTPAALDD329 pKa = 3.51TTTPSGPVVTTPSGPEE345 pKa = 3.7TTTAPCGHH353 pKa = 7.46DD354 pKa = 3.06EE355 pKa = 4.35TTPSEE360 pKa = 4.37TDD362 pKa = 3.23TTPAALDD369 pKa = 3.51TTTPSGPVVTTPSGPEE385 pKa = 3.7TTTAPCGHH393 pKa = 7.46DD394 pKa = 3.06EE395 pKa = 4.35TTPSEE400 pKa = 4.37TDD402 pKa = 3.23TTPAALDD409 pKa = 3.51TTTPSGPVVTTPSGPEE425 pKa = 3.7TTTAPCGHH433 pKa = 7.46DD434 pKa = 3.06EE435 pKa = 4.35TTPSEE440 pKa = 4.37TDD442 pKa = 3.23TTPAALDD449 pKa = 3.51TTTPSGPVVTTPSGPEE465 pKa = 3.7TTTAPCGHH473 pKa = 7.46DD474 pKa = 3.06EE475 pKa = 4.35TTPSEE480 pKa = 4.37TDD482 pKa = 3.23TTPAALDD489 pKa = 3.51TTTPSGPVVTTPSGPEE505 pKa = 3.7TTTAPCGHH513 pKa = 7.46DD514 pKa = 3.06EE515 pKa = 4.35TTPSEE520 pKa = 4.37TDD522 pKa = 3.23TTPAALDD529 pKa = 3.51TTTPSGPVVTTPSGPEE545 pKa = 3.7TTTAPCGHH553 pKa = 7.46DD554 pKa = 3.06EE555 pKa = 4.35TTPSEE560 pKa = 4.37TDD562 pKa = 3.23TTPAALDD569 pKa = 3.51TTTPSGPVVTTPSGPEE585 pKa = 3.7TTTAPCGHH593 pKa = 7.46DD594 pKa = 3.06EE595 pKa = 4.35TTPSEE600 pKa = 4.37TDD602 pKa = 3.23TTPAALDD609 pKa = 3.51TTTPSGPVVTTPSGPEE625 pKa = 3.7TTTAPCGHH633 pKa = 7.46DD634 pKa = 3.06EE635 pKa = 4.35TTPSEE640 pKa = 4.37TDD642 pKa = 3.23TTPAALDD649 pKa = 3.51TTTPSGPVVTTPSGPEE665 pKa = 3.7TTTAPCGHH673 pKa = 7.46DD674 pKa = 3.06EE675 pKa = 4.35TTPSEE680 pKa = 4.37TDD682 pKa = 3.23TTPAALDD689 pKa = 3.51TTTPSGPVVTTPSGPEE705 pKa = 3.7TTTAPCGHH713 pKa = 7.46DD714 pKa = 3.06EE715 pKa = 4.35TTPSEE720 pKa = 4.37TDD722 pKa = 3.23TTPAALDD729 pKa = 3.51TTTPSGPVVTTPSGPEE745 pKa = 3.7TTTAPCGHH753 pKa = 7.46DD754 pKa = 3.06EE755 pKa = 4.35TTPSEE760 pKa = 4.37TDD762 pKa = 3.23TTPAALDD769 pKa = 3.51TTTPSGPVVTTPSGPEE785 pKa = 3.7TTTAPCGHH793 pKa = 7.46DD794 pKa = 3.06EE795 pKa = 4.35TTPSEE800 pKa = 4.37TDD802 pKa = 3.23TTPAALDD809 pKa = 3.51TTTPSGPVVTTPSGPEE825 pKa = 3.7TTTAPCGHH833 pKa = 7.46DD834 pKa = 3.06EE835 pKa = 4.35TTPSEE840 pKa = 4.37TDD842 pKa = 3.23TTPAALDD849 pKa = 3.51TTTPSGPVVTTPSGPEE865 pKa = 3.7TTTAPCGHH873 pKa = 7.46DD874 pKa = 3.06EE875 pKa = 4.35TTPSEE880 pKa = 4.37TDD882 pKa = 3.3TTPAAPDD889 pKa = 3.38TTTPIGPEE897 pKa = 3.31ITTPGGPEE905 pKa = 3.68VTTPGGPEE913 pKa = 3.29ITTPGGPEE921 pKa = 3.27ITTPGGPEE929 pKa = 3.27ITTPGGPEE937 pKa = 3.68VTTPGGPEE945 pKa = 3.68VTTPGGPEE953 pKa = 3.69VTTPSGPEE961 pKa = 3.24ITTPGGPEE969 pKa = 3.27ITTPGGPEE977 pKa = 3.68VTTPGGPEE985 pKa = 3.29ITTPGGPEE993 pKa = 3.46ITTSGGPEE1001 pKa = 3.43ITTPGGPEE1009 pKa = 3.58KK1010 pKa = 8.62TTPSGPEE1017 pKa = 3.68TTTAPCGHH1025 pKa = 7.46DD1026 pKa = 3.06EE1027 pKa = 4.35TTPSEE1032 pKa = 4.37TDD1034 pKa = 3.23TTPAALDD1041 pKa = 3.51TTTPSGPVVTTPSGPEE1057 pKa = 3.7TTTAPCGHH1065 pKa = 7.46DD1066 pKa = 3.06EE1067 pKa = 4.35TTPSEE1072 pKa = 4.37TDD1074 pKa = 3.23TTPAALDD1081 pKa = 3.47TTTPSGSVVTTPSGPEE1097 pKa = 3.7TTTAPCGHH1105 pKa = 7.46DD1106 pKa = 3.06EE1107 pKa = 4.35TTPSEE1112 pKa = 4.37TDD1114 pKa = 3.23TTPAALDD1121 pKa = 3.47TTTPSGSVVTTPSGPEE1137 pKa = 3.7TTTAPCGHH1145 pKa = 7.46DD1146 pKa = 3.06EE1147 pKa = 4.35TTPSEE1152 pKa = 4.37TDD1154 pKa = 3.23TTPAALDD1161 pKa = 3.49TTTPSGLVVTTPSGPEE1177 pKa = 3.7TTTAPCGHH1185 pKa = 7.46DD1186 pKa = 3.06EE1187 pKa = 4.35TTPSEE1192 pKa = 4.37TDD1194 pKa = 3.3TTPAAPDD1201 pKa = 3.41TTTPSGPVVTTPSGPEE1217 pKa = 3.7TTTAPCGHH1225 pKa = 7.46DD1226 pKa = 3.06EE1227 pKa = 4.35TTPSEE1232 pKa = 4.44TGTTPAAPDD1241 pKa = 3.01ITTPSGPEE1249 pKa = 3.69TTTAPCGHH1257 pKa = 7.46DD1258 pKa = 3.06EE1259 pKa = 4.35TTPSEE1264 pKa = 4.44TGTTPAAPDD1273 pKa = 3.45TTTPSGPVVTTPSGPEE1289 pKa = 3.7TTTAPCGHH1297 pKa = 7.46DD1298 pKa = 3.06EE1299 pKa = 4.35TTPSEE1304 pKa = 4.37TDD1306 pKa = 3.3TTPAAPDD1313 pKa = 3.41TTTPSGPVVTTPSGPEE1329 pKa = 3.7TTTAPCGHH1337 pKa = 7.46DD1338 pKa = 3.06EE1339 pKa = 4.35TTPSEE1344 pKa = 4.44TGTTPAAPDD1353 pKa = 3.45TTTPSGPEE1361 pKa = 3.77TTTAPCGHH1369 pKa = 7.46DD1370 pKa = 3.06EE1371 pKa = 4.35TTPSEE1376 pKa = 4.44TGTTPAAPDD1385 pKa = 3.45TTTPSGPVVTTPSGPEE1401 pKa = 3.7TTTAPCGHH1409 pKa = 7.46DD1410 pKa = 3.06EE1411 pKa = 4.35TTPSEE1416 pKa = 4.37TDD1418 pKa = 3.3TTPAAPDD1425 pKa = 3.41TTTPSGPVVTTPSGPEE1441 pKa = 3.7TTTAPCGHH1449 pKa = 7.46DD1450 pKa = 3.06EE1451 pKa = 4.35TTPSEE1456 pKa = 4.37TDD1458 pKa = 3.3TTPAAPDD1465 pKa = 3.33TATPSGPEE1473 pKa = 4.12TTTSPCGHH1481 pKa = 7.45DD1482 pKa = 3.12EE1483 pKa = 4.31TTPSEE1488 pKa = 4.37TDD1490 pKa = 3.23TTPAALDD1497 pKa = 3.49TTTPSGLVVTTPSGPEE1513 pKa = 3.7TTTAPCGHH1521 pKa = 7.49DD1522 pKa = 3.29EE1523 pKa = 4.29TTPSGPHH1530 pKa = 5.08VTTQSEE1536 pKa = 4.66PDD1538 pKa = 3.35TTTPRR1543 pKa = 11.84PATFPPITTPSSCQHH1558 pKa = 6.49HH1559 pKa = 6.47PTSEE1563 pKa = 4.07KK1564 pKa = 10.57PITSTSRR1571 pKa = 11.84PSTPPCGHH1579 pKa = 6.59SSTTKK1584 pKa = 8.97ATTIHH1589 pKa = 7.24RR1590 pKa = 11.84SGKK1593 pKa = 8.83PCKK1596 pKa = 10.08CSDD1599 pKa = 3.24GATTEE1604 pKa = 4.16VPTDD1608 pKa = 3.67VSDD1611 pKa = 3.94HH1612 pKa = 6.32LVDD1615 pKa = 3.84EE1616 pKa = 4.89TVHH1619 pKa = 6.38LLNTGVVAVEE1629 pKa = 4.35DD1630 pKa = 3.99LLEE1633 pKa = 4.23EE1634 pKa = 4.37LLASGQKK1641 pKa = 10.53DD1642 pKa = 3.0ILHH1645 pKa = 7.2KK1646 pKa = 10.24IRR1648 pKa = 11.84RR1649 pKa = 11.84TKK1651 pKa = 10.34RR1652 pKa = 11.84KK1653 pKa = 8.36NRR1655 pKa = 11.84RR1656 pKa = 11.84TRR1658 pKa = 11.84LHH1660 pKa = 6.51KK1661 pKa = 10.06PATVLPILII1670 pKa = 4.64

Molecular weight: 164.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A4K6K5|A0A2A4K6K5_HELVI CRF domain-containing protein OS=Heliothis virescens OX=7102 GN=B5V51_13338 PE=4 SV=1
EEE2 pKa = 4.37AATASPYYY10 pKa = 10.67LAASLRR16 pKa = 11.84RR17 pKa = 11.84TASSYYY23 pKa = 11.15LAAPPRR29 pKa = 11.84RR30 pKa = 11.84TTAPHHH36 pKa = 6.78FAALPRR42 pKa = 11.84RR43 pKa = 11.84TTEEE47 pKa = 3.43HHH49 pKa = 6.65FATLPRR55 pKa = 11.84RR56 pKa = 11.84TNAPHHH62 pKa = 7.16FAALPRR68 pKa = 11.84RR69 pKa = 11.84TTAPHHH75 pKa = 6.89FAAPPRR81 pKa = 11.84RR82 pKa = 11.84TASPHHH88 pKa = 6.26LAAPPRR94 pKa = 11.84RR95 pKa = 11.84TASPHHH101 pKa = 6.19LAAPPHHH108 pKa = 5.94TASPHHH114 pKa = 5.78HH115 pKa = 6.47AAPLRR120 pKa = 11.84RR121 pKa = 11.84TASPHHH127 pKa = 6.73LTAPPRR133 pKa = 11.84RR134 pKa = 11.84TTAPHHH140 pKa = 6.89FAAPPRR146 pKa = 11.84RR147 pKa = 11.84TASPHHH153 pKa = 6.26LAAPPRR159 pKa = 11.84RR160 pKa = 11.84TASPHHH166 pKa = 6.19LAAPPHHH173 pKa = 5.94TASPHHH179 pKa = 5.78HH180 pKa = 6.47AAPLRR185 pKa = 11.84RR186 pKa = 11.84TASPHHH192 pKa = 6.73LTAPPRR198 pKa = 11.84RR199 pKa = 11.84TTAPHHH205 pKa = 6.89FAAPPRR211 pKa = 11.84RR212 pKa = 11.84TASPHHH218 pKa = 6.26LAAPPRR224 pKa = 11.84RR225 pKa = 11.84TASPHHH231 pKa = 6.19LAAPPHHH238 pKa = 5.94TASPHHH244 pKa = 5.78HH245 pKa = 6.47AAPLRR250 pKa = 11.84RR251 pKa = 11.84TASPHHH257 pKa = 6.73LTAPPRR263 pKa = 11.84RR264 pKa = 11.84TTAPHHH270 pKa = 6.89FAAPPRR276 pKa = 11.84RR277 pKa = 11.84TASPHHH283 pKa = 6.26LAAPPRR289 pKa = 11.84RR290 pKa = 11.84TASPHHH296 pKa = 6.19LAAPPHHH303 pKa = 5.94TASPHHH309 pKa = 5.78HH310 pKa = 6.47AAPLRR315 pKa = 11.84RR316 pKa = 11.84TASPHHH322 pKa = 6.73LTAPPRR328 pKa = 11.84RR329 pKa = 11.84TTAPHHH335 pKa = 6.89FAAPPRR341 pKa = 11.84RR342 pKa = 11.84TASPHHH348 pKa = 6.26LAAPPRR354 pKa = 11.84RR355 pKa = 11.84TASPHHH361 pKa = 6.19LAAPPHHH368 pKa = 5.94TASPHHH374 pKa = 5.78HH375 pKa = 6.47AAPLRR380 pKa = 11.84RR381 pKa = 11.84TASPHHH387 pKa = 6.73LTAPPRR393 pKa = 11.84RR394 pKa = 11.84TTAPHHH400 pKa = 6.89FAAPPRR406 pKa = 11.84RR407 pKa = 11.84TASPHHH413 pKa = 6.26LAAPPRR419 pKa = 11.84RR420 pKa = 11.84TASPHHH426 pKa = 6.19LAAPPHHH433 pKa = 5.94TASPHHH439 pKa = 5.78HH440 pKa = 6.47AAPLRR445 pKa = 11.84RR446 pKa = 11.84TASPHHH452 pKa = 6.73LTAPPRR458 pKa = 11.84RR459 pKa = 11.84TTAPHHH465 pKa = 6.89FAAPPRR471 pKa = 11.84RR472 pKa = 11.84TASPHHH478 pKa = 6.26LAAPPRR484 pKa = 11.84NPPSDDD490 pKa = 3.57PFAGRR495 pKa = 11.84IQRR498 pKa = 11.84MGEEE502 pKa = 3.95VVRR505 pKa = 11.84GRR507 pKa = 11.84

Molecular weight: 55.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14951

2104

17055

9040933

42

11599

530.1

59.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.453 ± 0.034

2.116 ± 0.041

5.622 ± 0.022

6.618 ± 0.034

3.312 ± 0.017

5.668 ± 0.034

2.56 ± 0.015

4.995 ± 0.02

6.168 ± 0.038

8.744 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.012

4.534 ± 0.022

5.902 ± 0.047

4.002 ± 0.021

5.84 ± 0.027

7.629 ± 0.027

5.995 ± 0.038

6.435 ± 0.019

1.07 ± 0.009

3.122 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski