Juglans regia (English walnut)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fagales; Juglandaceae; Juglans

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38418 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I4FJ54|A0A2I4FJ54_JUGRE FAM10 family protein At4g22670-like OS=Juglans regia OX=51240 GN=LOC108999267 PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.18KK3 pKa = 10.33CEE5 pKa = 4.36LCDD8 pKa = 3.74SPANLYY14 pKa = 10.59CEE16 pKa = 4.52SDD18 pKa = 3.59QASLCWDD25 pKa = 3.52CDD27 pKa = 3.6ARR29 pKa = 11.84VHH31 pKa = 6.31GANFLVAKK39 pKa = 10.33HH40 pKa = 6.1SRR42 pKa = 11.84TLLCHH47 pKa = 6.07VCQSSTPWNGSGPKK61 pKa = 9.79LGPTISVCEE70 pKa = 3.68ICVNSNVKK78 pKa = 10.47NEE80 pKa = 4.27AGNEE84 pKa = 4.2GNDD87 pKa = 3.77HH88 pKa = 7.52DD89 pKa = 4.89NGADD93 pKa = 3.6GVGDD97 pKa = 4.23SDD99 pKa = 6.54DD100 pKa = 5.33DD101 pKa = 6.23DD102 pKa = 6.33SDD104 pKa = 4.59SDD106 pKa = 4.42DD107 pKa = 3.71NFEE110 pKa = 5.13EE111 pKa = 4.93EE112 pKa = 4.96EE113 pKa = 4.71DD114 pKa = 5.49DD115 pKa = 6.32DD116 pKa = 4.85GQGDD120 pKa = 3.68NDD122 pKa = 3.57EE123 pKa = 5.39DD124 pKa = 3.99EE125 pKa = 5.19EE126 pKa = 4.47EE127 pKa = 4.33NQVVPWSSTPPPPTSSSSWRR147 pKa = 11.84GIHH150 pKa = 6.58

Molecular weight: 16.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P9DV69|A0A6P9DV69_JUGRE uncharacterized protein LOC109011743 OS=Juglans regia OX=51240 GN=LOC109011743 PE=4 SV=1
MM1 pKa = 7.48AHH3 pKa = 7.41LLLEE7 pKa = 4.52SLQKK11 pKa = 10.48GAPISPSVPNPTIPPSLSTISQRR34 pKa = 11.84TFTSHH39 pKa = 6.47PRR41 pKa = 11.84AHH43 pKa = 7.11LLGTVLQKK51 pKa = 10.94GHH53 pKa = 7.07IPPSAPNPPIPPRR66 pKa = 11.84LSTISQRR73 pKa = 11.84AFASHH78 pKa = 6.85PRR80 pKa = 11.84AHH82 pKa = 6.86LLWTILQRR90 pKa = 11.84GHH92 pKa = 6.96IPPPAPNPTIPPRR105 pKa = 11.84LSTINQKK112 pKa = 10.77AFGSHH117 pKa = 6.0PRR119 pKa = 11.84AHH121 pKa = 7.12LLGTVLQKK129 pKa = 10.91GHH131 pKa = 7.05IPPPGPNSKK140 pKa = 9.78IPRR143 pKa = 11.84HH144 pKa = 6.05LSTISQRR151 pKa = 11.84AFASHH156 pKa = 6.86PRR158 pKa = 11.84AHH160 pKa = 7.2LLGTVLQRR168 pKa = 11.84GHH170 pKa = 6.97IPPSVPNPPILPRR183 pKa = 11.84LSTISQRR190 pKa = 11.84AFASHH195 pKa = 6.85PRR197 pKa = 11.84AHH199 pKa = 6.86LLWTILQRR207 pKa = 11.84GPIPPPAPNPTIPPRR222 pKa = 11.84LSTINQKK229 pKa = 10.77AFGSHH234 pKa = 6.0PRR236 pKa = 11.84AHH238 pKa = 7.12LLGTVLQKK246 pKa = 10.91GHH248 pKa = 7.05IPPPGPNSKK257 pKa = 9.78IPRR260 pKa = 11.84HH261 pKa = 6.05LSTISQRR268 pKa = 11.84AFASHH273 pKa = 6.86PRR275 pKa = 11.84AHH277 pKa = 7.2LLGTVLQRR285 pKa = 11.84GHH287 pKa = 6.97IPPSVPNPTIPPRR300 pKa = 11.84FSTISQRR307 pKa = 11.84AFASHH312 pKa = 6.85PRR314 pKa = 11.84AHH316 pKa = 6.86LLWTVLQRR324 pKa = 11.84GHH326 pKa = 6.95IPPPAPNPTIPPRR339 pKa = 11.84LSTINQKK346 pKa = 10.77AFGSHH351 pKa = 6.0PRR353 pKa = 11.84AHH355 pKa = 7.09LLGTILQKK363 pKa = 10.59SHH365 pKa = 7.03IPPSAPNPTIPPLLSTINQKK385 pKa = 10.59AFASHH390 pKa = 6.77PRR392 pKa = 11.84AHH394 pKa = 7.2LLGTVLQKK402 pKa = 10.92GHH404 pKa = 7.03IPPSGPNSKK413 pKa = 9.73IPRR416 pKa = 11.84HH417 pKa = 5.69ISTLISQNNNN427 pKa = 2.76

Molecular weight: 46.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30331

8087

38418

18253930

17

6605

475.1

53.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.634 ± 0.011

1.878 ± 0.006

5.328 ± 0.008

6.605 ± 0.022

4.17 ± 0.009

6.539 ± 0.01

2.439 ± 0.005

5.326 ± 0.01

6.025 ± 0.012

9.783 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.005

4.449 ± 0.008

4.901 ± 0.011

3.745 ± 0.008

5.39 ± 0.011

9.036 ± 0.014

4.848 ± 0.008

6.423 ± 0.008

1.302 ± 0.005

2.773 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski