Hirundo rustica rustica

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria;

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34534 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M0II28|A0A3M0II28_HIRRU Uncharacterized protein OS=Hirundo rustica rustica OX=333673 GN=DUI87_35224 PE=4 SV=1
MM1 pKa = 7.58QEE3 pKa = 3.73QTEE6 pKa = 4.23RR7 pKa = 11.84APTDD11 pKa = 3.65WDD13 pKa = 3.62HH14 pKa = 6.72MDD16 pKa = 3.67WDD18 pKa = 4.16PTDD21 pKa = 3.51WACTDD26 pKa = 3.96RR27 pKa = 11.84DD28 pKa = 4.08RR29 pKa = 11.84MDD31 pKa = 3.63QEE33 pKa = 4.55HH34 pKa = 7.63PDD36 pKa = 3.42WDD38 pKa = 4.02DD39 pKa = 4.16ADD41 pKa = 3.8WDD43 pKa = 4.29LVDD46 pKa = 4.19WDD48 pKa = 4.13CTGWAHH54 pKa = 6.55VDD56 pKa = 2.74WDD58 pKa = 4.04FTDD61 pKa = 3.7WDD63 pKa = 4.12CMGWAHH69 pKa = 6.62MDD71 pKa = 2.93WDD73 pKa = 4.19FTDD76 pKa = 3.86WDD78 pKa = 3.93CMGWVHH84 pKa = 7.25LDD86 pKa = 2.86WDD88 pKa = 4.15FTDD91 pKa = 3.75WDD93 pKa = 4.15CTHH96 pKa = 7.72WDD98 pKa = 3.43PMDD101 pKa = 3.42WDD103 pKa = 3.93FTGWDD108 pKa = 4.23HH109 pKa = 7.81LDD111 pKa = 3.39QNPRR115 pKa = 11.84EE116 pKa = 4.07WDD118 pKa = 3.36HH119 pKa = 7.12ANWDD123 pKa = 3.64HH124 pKa = 6.82MDD126 pKa = 3.46QDD128 pKa = 4.1GCTVTMM134 pKa = 5.02

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M0KQ79|A0A3M0KQ79_HIRRU PlsC domain-containing protein OS=Hirundo rustica rustica OX=333673 GN=DUI87_07579 PE=4 SV=1
MM1 pKa = 7.94PEE3 pKa = 3.13ITVFKK8 pKa = 10.66KK9 pKa = 10.66AMSEE13 pKa = 4.1DD14 pKa = 3.79YY15 pKa = 10.99VLPEE19 pKa = 4.22LQNTRR24 pKa = 11.84IGIWIAIHH32 pKa = 4.72IRR34 pKa = 11.84IWIGIGIRR42 pKa = 11.84IGIGIRR48 pKa = 11.84IGIGIGIRR56 pKa = 11.84IGIRR60 pKa = 11.84IGIRR64 pKa = 11.84IGIGIRR70 pKa = 11.84IGIRR74 pKa = 11.84IRR76 pKa = 11.84IGIGIRR82 pKa = 11.84IGIRR86 pKa = 11.84IGIGIGIGIRR96 pKa = 11.84IRR98 pKa = 11.84IRR100 pKa = 11.84IRR102 pKa = 11.84IGIRR106 pKa = 11.84IGIRR110 pKa = 11.84IGIRR114 pKa = 11.84IGIGIRR120 pKa = 11.84IGIRR124 pKa = 11.84IGIGIRR130 pKa = 11.84IGIGIGIRR138 pKa = 11.84IGIRR142 pKa = 11.84IRR144 pKa = 11.84IGIGIRR150 pKa = 11.84IGIGIRR156 pKa = 11.84IRR158 pKa = 11.84IRR160 pKa = 11.84IGIGIRR166 pKa = 11.84IRR168 pKa = 11.84IGIGIGIRR176 pKa = 11.84IGIGIRR182 pKa = 11.84IRR184 pKa = 11.84IGIGIGIRR192 pKa = 11.84IGIGLGFGIGIRR204 pKa = 11.84IGIGIGIGRR213 pKa = 11.84LRR215 pKa = 11.84IGIGIGIGIRR225 pKa = 11.84IGIGIGIGIRR235 pKa = 11.84IGIGLGIRR243 pKa = 11.84IRR245 pKa = 11.84IRR247 pKa = 11.84IGIGIGIRR255 pKa = 11.84IRR257 pKa = 11.84IRR259 pKa = 11.84IKK261 pKa = 10.34IRR263 pKa = 11.84ITLQVRR269 pKa = 11.84GG270 pKa = 3.88

Molecular weight: 28.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34534

0

34534

15462943

66

11093

447.8

50.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.152 ± 0.016

2.077 ± 0.01

4.731 ± 0.011

7.529 ± 0.016

3.344 ± 0.008

6.773 ± 0.016

2.638 ± 0.008

4.67 ± 0.011

5.908 ± 0.015

9.567 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.006

3.491 ± 0.01

6.024 ± 0.014

4.949 ± 0.012

5.981 ± 0.016

7.544 ± 0.016

5.291 ± 0.014

6.115 ± 0.015

1.698 ± 0.008

2.405 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski