Streptococcus satellite phage Javan219

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLT2|A0A4D5ZLT2_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan219 OX=2558576 GN=JavanS219_0003 PE=4 SV=1
MM1 pKa = 7.37YY2 pKa = 9.74EE3 pKa = 3.89QEE5 pKa = 4.58KK6 pKa = 10.89DD7 pKa = 3.62CIYY10 pKa = 11.61NMIDD14 pKa = 3.33LADD17 pKa = 3.77NAILQGDD24 pKa = 4.13KK25 pKa = 11.22DD26 pKa = 3.71HH27 pKa = 7.22ALTSLYY33 pKa = 10.25FIKK36 pKa = 10.54KK37 pKa = 9.21GLEE40 pKa = 3.86SLIVGLEE47 pKa = 3.71ADD49 pKa = 3.76NDD51 pKa = 3.75QQ52 pKa = 4.28

Molecular weight: 5.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZHJ3|A0A4D5ZHJ3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan219 OX=2558576 GN=JavanS219_0011 PE=4 SV=1
MM1 pKa = 7.59ANIKK5 pKa = 10.34KK6 pKa = 8.2ITKK9 pKa = 10.07KK10 pKa = 10.66NGTTVYY16 pKa = 10.47RR17 pKa = 11.84EE18 pKa = 3.94QIYY21 pKa = 10.81LGTDD25 pKa = 3.2CMTGKK30 pKa = 9.75QVYY33 pKa = 6.43TTISAPTKK41 pKa = 10.52KK42 pKa = 9.9EE43 pKa = 3.83LKK45 pKa = 9.55QKK47 pKa = 10.85RR48 pKa = 11.84EE49 pKa = 3.75FKK51 pKa = 10.77INEE54 pKa = 3.93FKK56 pKa = 11.26DD57 pKa = 3.34NGFTRR62 pKa = 11.84YY63 pKa = 10.24KK64 pKa = 10.46SVTVKK69 pKa = 10.57NYY71 pKa = 10.67RR72 pKa = 11.84EE73 pKa = 4.21LSEE76 pKa = 5.18LWLKK80 pKa = 8.48NHH82 pKa = 6.28KK83 pKa = 10.74LEE85 pKa = 4.9VKK87 pKa = 9.49PQTYY91 pKa = 9.64SQTVSEE97 pKa = 4.98LRR99 pKa = 11.84THH101 pKa = 7.23LLPVFGDD108 pKa = 3.42MKK110 pKa = 10.62VEE112 pKa = 5.01RR113 pKa = 11.84ITLPMVQEE121 pKa = 4.52FVNKK125 pKa = 9.93LASNDD130 pKa = 3.17KK131 pKa = 10.38LGRR134 pKa = 11.84VSFRR138 pKa = 11.84IILSINKK145 pKa = 9.73RR146 pKa = 11.84ILKK149 pKa = 9.77YY150 pKa = 10.46AVNLQIINVNPADD163 pKa = 4.14NIIVPKK169 pKa = 9.96NKK171 pKa = 10.1KK172 pKa = 9.99NISKK176 pKa = 10.5KK177 pKa = 10.08KK178 pKa = 7.76EE179 pKa = 3.74LKK181 pKa = 10.58FFEE184 pKa = 4.34TSQLKK189 pKa = 9.16QFKK192 pKa = 10.04DD193 pKa = 3.91YY194 pKa = 11.32LDD196 pKa = 4.06SLPNTFKK203 pKa = 11.05NYY205 pKa = 7.3YY206 pKa = 9.97HH207 pKa = 6.4KK208 pKa = 9.53TLYY211 pKa = 9.35LTLLSTGLRR220 pKa = 11.84IGEE223 pKa = 4.06AVALEE228 pKa = 4.23WSDD231 pKa = 3.49IDD233 pKa = 5.42LDD235 pKa = 3.87NGYY238 pKa = 9.85IDD240 pKa = 3.68VNKK243 pKa = 9.27TVAFSRR249 pKa = 11.84MEE251 pKa = 4.17TNSTKK256 pKa = 10.52SEE258 pKa = 3.8AGNRR262 pKa = 11.84KK263 pKa = 9.21ISIDD267 pKa = 3.55KK268 pKa = 9.11NTVLMLRR275 pKa = 11.84LYY277 pKa = 10.46KK278 pKa = 10.3ARR280 pKa = 11.84QYY282 pKa = 10.61QCFMEE287 pKa = 4.79HH288 pKa = 6.9GYY290 pKa = 9.46SSKK293 pKa = 8.75MAKK296 pKa = 9.98YY297 pKa = 10.11VFSNGFNIYY306 pKa = 9.94PNRR309 pKa = 11.84TNLQLVLTKK318 pKa = 10.25HH319 pKa = 6.32LKK321 pKa = 9.26QAGLPRR327 pKa = 11.84FTFHH331 pKa = 7.9AFRR334 pKa = 11.84HH335 pKa = 4.69THH337 pKa = 6.92ASLLLNAGISYY348 pKa = 10.65KK349 pKa = 10.3EE350 pKa = 3.81LQHH353 pKa = 6.91RR354 pKa = 11.84LGHH357 pKa = 5.56STLAMTMDD365 pKa = 4.15IYY367 pKa = 11.72SHH369 pKa = 6.87LSKK372 pKa = 10.68EE373 pKa = 4.28KK374 pKa = 10.45EE375 pKa = 3.92KK376 pKa = 10.83EE377 pKa = 3.86AVNFFEE383 pKa = 5.78KK384 pKa = 10.86AMANLL389 pKa = 3.66

Molecular weight: 45.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

2887

45

476

180.4

20.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.399 ± 0.44

0.693 ± 0.207

5.819 ± 0.658

8.105 ± 0.457

3.879 ± 0.348

4.711 ± 0.282

1.663 ± 0.31

7.239 ± 0.424

10.253 ± 0.499

9.837 ± 0.757

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.009 ± 0.273

6.373 ± 0.419

2.667 ± 0.336

3.879 ± 0.235

4.157 ± 0.52

6.858 ± 0.588

6.027 ± 0.656

5.438 ± 0.393

0.97 ± 0.144

5.023 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski