Tortoise microvirus 2

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W960|A0A4P8W960_9VIRU Uncharacterized protein OS=Tortoise microvirus 2 OX=2583121 PE=4 SV=1
MM1 pKa = 7.75SDD3 pKa = 4.48FIPVDD8 pKa = 4.19FFTDD12 pKa = 3.16DD13 pKa = 3.44GYY15 pKa = 11.87APVPVRR21 pKa = 11.84YY22 pKa = 8.71LASPEE27 pKa = 3.86TAEE30 pKa = 3.88VFNRR34 pKa = 11.84VMSMAPTFFPDD45 pKa = 4.22LPVDD49 pKa = 4.28ANPVIAEE56 pKa = 3.94QQYY59 pKa = 11.45AMALVTLAVNFYY71 pKa = 10.79FDD73 pKa = 3.9TLGLLSPPEE82 pKa = 4.25VAPSTPVGDD91 pKa = 4.38DD92 pKa = 3.4DD93 pKa = 6.38LNIKK97 pKa = 9.51EE98 pKa = 4.31SHH100 pKa = 4.82VV101 pKa = 3.46

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FVV9|A0A4V1FVV9_9VIRU Uncharacterized protein OS=Tortoise microvirus 2 OX=2583121 PE=4 SV=1
MM1 pKa = 7.04TSVMSHH7 pKa = 6.96PSQFFMILPNDD18 pKa = 3.99VYY20 pKa = 11.25RR21 pKa = 11.84LGLNPAKK28 pKa = 9.9IARR31 pKa = 11.84MAYY34 pKa = 9.9LYY36 pKa = 11.26AHH38 pKa = 7.36IADD41 pKa = 4.91CLPSDD46 pKa = 4.33VTCSPPLHH54 pKa = 6.47CTTPSLLAQRR64 pKa = 11.84AKK66 pKa = 10.77SSTGVSYY73 pKa = 11.11VV74 pKa = 2.98

Molecular weight: 8.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1885

74

584

209.4

23.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.692 ± 1.065

1.432 ± 0.541

6.578 ± 0.474

4.35 ± 0.692

5.146 ± 0.488

5.889 ± 0.686

1.804 ± 0.282

4.191 ± 0.61

3.607 ± 1.148

9.231 ± 0.978

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.865 ± 0.324

4.721 ± 0.29

5.995 ± 0.741

4.403 ± 1.571

5.517 ± 0.467

8.806 ± 1.08

5.942 ± 0.521

6.26 ± 0.706

0.584 ± 0.16

4.987 ± 0.704
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski