Jannaschia donghaensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Jannaschia

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M6YE48|A0A0M6YE48_9RHOB Succinate-semialdehyde dehydrogenase [NADP(+)] 1 OS=Jannaschia donghaensis OX=420998 GN=gabD1 PE=4 SV=1
MM1 pKa = 7.13TRR3 pKa = 11.84SKK5 pKa = 10.91FLGSTFCLASLLAAPTTAQEE25 pKa = 4.3RR26 pKa = 11.84PLTVAMHH33 pKa = 5.54YY34 pKa = 7.74TQEE37 pKa = 3.85QAAPLIACLDD47 pKa = 3.77AYY49 pKa = 9.95GTDD52 pKa = 3.93GPGAEE57 pKa = 4.37YY58 pKa = 10.44QQISYY63 pKa = 11.06GDD65 pKa = 3.65YY66 pKa = 10.52LQTVLTGRR74 pKa = 11.84LAGQAPDD81 pKa = 3.21IYY83 pKa = 10.92NVYY86 pKa = 10.21SIWAAQMVDD95 pKa = 3.24NGVLATPPAAVTDD108 pKa = 4.44FVTAGYY114 pKa = 9.97SAGTVDD120 pKa = 4.69AATIDD125 pKa = 3.68GTLWGVPTEE134 pKa = 4.05VSVYY138 pKa = 9.89MLVSNMALLRR148 pKa = 11.84EE149 pKa = 4.57AGFDD153 pKa = 3.67TPPTTWAEE161 pKa = 4.06MRR163 pKa = 11.84EE164 pKa = 4.03MAQAITTRR172 pKa = 11.84NDD174 pKa = 2.67QDD176 pKa = 3.93RR177 pKa = 11.84IEE179 pKa = 4.22TAGFAFAQSSSGAGLVHH196 pKa = 7.03PFYY199 pKa = 11.23ALTYY203 pKa = 10.15SEE205 pKa = 5.37GGDD208 pKa = 3.0IYY210 pKa = 11.4ADD212 pKa = 3.69DD213 pKa = 4.35RR214 pKa = 11.84SEE216 pKa = 5.37AMLEE220 pKa = 3.92SDD222 pKa = 4.87AATATAGLMAGMVQDD237 pKa = 5.55GITDD241 pKa = 4.32LSVDD245 pKa = 4.04AYY247 pKa = 10.57DD248 pKa = 5.01FPAGGIGMMIMANWYY263 pKa = 9.02EE264 pKa = 4.05SAIRR268 pKa = 11.84EE269 pKa = 4.37GFGDD273 pKa = 4.53RR274 pKa = 11.84FDD276 pKa = 4.47EE277 pKa = 4.54DD278 pKa = 4.12VAVSAIPMGEE288 pKa = 4.04DD289 pKa = 2.85WKK291 pKa = 9.71TMQYY295 pKa = 11.21AFFMGVDD302 pKa = 3.76SASDD306 pKa = 3.25RR307 pKa = 11.84SDD309 pKa = 3.51AAWDD313 pKa = 3.67LVRR316 pKa = 11.84HH317 pKa = 5.6LNEE320 pKa = 4.6PRR322 pKa = 11.84DD323 pKa = 3.86GAPSCMAEE331 pKa = 4.04MLDD334 pKa = 4.15GLGALTASSADD345 pKa = 3.41TAALPEE351 pKa = 4.67ADD353 pKa = 4.74AFTAPFVAALADD365 pKa = 3.9DD366 pKa = 4.32RR367 pKa = 11.84AISQPNVMQASEE379 pKa = 4.63LEE381 pKa = 4.08GLIARR386 pKa = 11.84TLEE389 pKa = 3.93EE390 pKa = 4.64VIVGDD395 pKa = 4.37AEE397 pKa = 4.32AGPAMEE403 pKa = 5.11EE404 pKa = 3.84LDD406 pKa = 4.28GDD408 pKa = 4.09VEE410 pKa = 5.22DD411 pKa = 4.87ILSEE415 pKa = 4.68FYY417 pKa = 11.22

Molecular weight: 44.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M6YI42|A0A0M6YI42_9RHOB YGGT family protein OS=Jannaschia donghaensis OX=420998 GN=JDO7802_00945 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.08AGRR28 pKa = 11.84RR29 pKa = 11.84ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3480

0

3480

1044310

30

4206

300.1

32.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.849 ± 0.059

0.86 ± 0.013

6.893 ± 0.045

5.213 ± 0.045

3.581 ± 0.025

8.957 ± 0.046

2.014 ± 0.022

5.02 ± 0.029

2.648 ± 0.038

9.965 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.795 ± 0.021

2.307 ± 0.023

5.247 ± 0.031

2.917 ± 0.023

7.034 ± 0.051

4.707 ± 0.029

6.025 ± 0.046

7.533 ± 0.038

1.444 ± 0.02

1.992 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski