Portunus trituberculatus (Swimming crab) (Neptunus trituberculatus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Brachyura; Eubrachyura; Heterotremata; Po

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B7K3V9|A0A5B7K3V9_PORTR Uncharacterized protein OS=Portunus trituberculatus OX=210409 GN=E2C01_097197 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.37NKK5 pKa = 10.14TSPHH9 pKa = 6.32RR10 pKa = 11.84GGTVVMVKK18 pKa = 10.53NYY20 pKa = 9.65LAQSAMNVDD29 pKa = 3.86TSTADD34 pKa = 2.94QSYY37 pKa = 9.35LHH39 pKa = 6.31EE40 pKa = 4.38QSCLTNTDD48 pKa = 3.36LTDD51 pKa = 3.68IDD53 pKa = 4.21LTDD56 pKa = 3.55VDD58 pKa = 4.4VLASNVANMLYY69 pKa = 9.61EE70 pKa = 4.39CSEE73 pKa = 4.08ASQVPVAVANLVSGGNRR90 pKa = 11.84WDD92 pKa = 4.21RR93 pKa = 11.84LLSDD97 pKa = 4.52PDD99 pKa = 3.52DD100 pKa = 3.7TRR102 pKa = 11.84VWKK105 pKa = 10.37AIPSDD110 pKa = 3.76EE111 pKa = 4.17FKK113 pKa = 10.91SYY115 pKa = 11.74YY116 pKa = 9.08EE117 pKa = 4.24TFCNPGIDD125 pKa = 5.15DD126 pKa = 4.94DD127 pKa = 5.18FNYY130 pKa = 10.85SNDD133 pKa = 3.51SPYY136 pKa = 10.93VPILDD141 pKa = 4.32DD142 pKa = 4.35VITVDD147 pKa = 4.18EE148 pKa = 4.93LSSTSDD154 pKa = 3.63SEE156 pKa = 4.82NEE158 pKa = 3.98TT159 pKa = 3.74

Molecular weight: 17.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B7I464|A0A5B7I464_PORTR Uncharacterized protein OS=Portunus trituberculatus OX=210409 GN=E2C01_070071 PE=4 SV=1
MM1 pKa = 7.56AAQISKK7 pKa = 10.41HH8 pKa = 4.46SARR11 pKa = 11.84AIRR14 pKa = 11.84FMARR18 pKa = 11.84FTVRR22 pKa = 11.84VLVGVTRR29 pKa = 11.84PPHH32 pKa = 5.42AAPRR36 pKa = 11.84PRR38 pKa = 11.84RR39 pKa = 11.84PQSRR43 pKa = 11.84AGRR46 pKa = 11.84AAGRR50 pKa = 11.84FWPARR55 pKa = 11.84RR56 pKa = 11.84GKK58 pKa = 9.27QQ59 pKa = 3.0

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99293

127

99420

13516514

8

5097

136.0

15.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.991 ± 0.012

2.538 ± 0.009

3.98 ± 0.01

5.747 ± 0.016

3.074 ± 0.008

6.81 ± 0.015

3.513 ± 0.008

3.736 ± 0.008

4.567 ± 0.013

9.175 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.005

3.215 ± 0.008

6.954 ± 0.016

3.988 ± 0.01

7.181 ± 0.012

9.084 ± 0.017

6.693 ± 0.013

6.636 ± 0.013

1.365 ± 0.004

2.288 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski