Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3KSS2|Q3KSS2_EBVG Uracil-DNA glycosylase OS=Epstein-Barr virus (strain GD1) OX=10376 GN=BKRF3 PE=3 SV=1
MM1 pKa = 7.14GALWSLCRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84VNSIGDD17 pKa = 3.37VDD19 pKa = 3.76GGIINLYY26 pKa = 10.26NDD28 pKa = 3.48YY29 pKa = 11.59EE30 pKa = 4.25EE31 pKa = 5.69FNLEE35 pKa = 4.1TTKK38 pKa = 11.06LIAVEE43 pKa = 4.06EE44 pKa = 4.54GRR46 pKa = 11.84ACGEE50 pKa = 4.08TNEE53 pKa = 4.04GLEE56 pKa = 3.96YY57 pKa = 11.13DD58 pKa = 4.15EE59 pKa = 6.6DD60 pKa = 4.32SEE62 pKa = 4.75NDD64 pKa = 3.38EE65 pKa = 6.11LLFLPNKK72 pKa = 9.92KK73 pKa = 10.13PNN75 pKa = 3.37

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5KTU9|V5KTU9_EBVG Nuclear egress protein 2 OS=Epstein-Barr virus (strain GD1) OX=10376 GN=BFRF1 PE=1 SV=1
MM1 pKa = 7.61SMPPKK6 pKa = 10.67GFLKK10 pKa = 10.66KK11 pKa = 10.15EE12 pKa = 4.14MKK14 pKa = 10.36PEE16 pKa = 3.61TRR18 pKa = 11.84LLNKK22 pKa = 9.83PPTVLTRR29 pKa = 11.84PAMFCAWKK37 pKa = 10.15LYY39 pKa = 8.25SRR41 pKa = 11.84KK42 pKa = 8.88MPSRR46 pKa = 11.84SKK48 pKa = 9.67TLEE51 pKa = 3.91ARR53 pKa = 11.84CSSRR57 pKa = 11.84PPCDD61 pKa = 3.76SPACQTRR68 pKa = 11.84DD69 pKa = 3.11TGCPRR74 pKa = 11.84RR75 pKa = 11.84SGTGRR80 pKa = 11.84RR81 pKa = 11.84GWRR84 pKa = 11.84ARR86 pKa = 11.84RR87 pKa = 11.84LGKK90 pKa = 10.31EE91 pKa = 3.78SWFADD96 pKa = 3.34AWRR99 pKa = 11.84MARR102 pKa = 11.84YY103 pKa = 7.98WGCAVKK109 pKa = 10.29AAAQSAFSASTASPEE124 pKa = 4.01EE125 pKa = 4.06LL126 pKa = 3.81

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

3755

60

924

234.7

25.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.493 ± 2.241

2.077 ± 0.564

4.154 ± 0.467

4.794 ± 0.591

3.169 ± 0.775

7.084 ± 0.668

2.77 ± 0.374

2.903 ± 0.655

3.595 ± 0.887

9.587 ± 1.5

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.758 ± 0.453

3.249 ± 0.891

10.2 ± 3.585

3.169 ± 0.248

8.043 ± 1.661

7.27 ± 1.154

6.551 ± 1.39

6.178 ± 0.82

1.145 ± 0.394

1.811 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski