Odonata associated gemycircularvirus-2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus odona2; Odonata associated gemycircularvirus 2

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UH04|A0A0B4UH04_9VIRU Replication-associated protein OS=Odonata associated gemycircularvirus-2 OX=1592108 PE=3 SV=1
MM1 pKa = 7.86APFHH5 pKa = 6.32LKK7 pKa = 9.3NRR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 10.35VLLTYY16 pKa = 10.3SQAGSEE22 pKa = 4.15FNYY25 pKa = 9.28WAIVDD30 pKa = 3.92MLSSHH35 pKa = 6.16GAEE38 pKa = 4.21CIIGRR43 pKa = 11.84EE44 pKa = 3.97LDD46 pKa = 3.55ADD48 pKa = 4.1GGTHH52 pKa = 5.57FHH54 pKa = 6.24VFVDD58 pKa = 4.51FGRR61 pKa = 11.84LFSTRR66 pKa = 11.84KK67 pKa = 8.19TNVFDD72 pKa = 4.58VDD74 pKa = 3.52GHH76 pKa = 6.54HH77 pKa = 7.3PNILPVWKK85 pKa = 8.83TPGEE89 pKa = 4.17AFDD92 pKa = 4.29YY93 pKa = 10.46AAKK96 pKa = 10.85DD97 pKa = 3.46GDD99 pKa = 3.89IVAGGLEE106 pKa = 4.42RR107 pKa = 11.84PGTDD111 pKa = 2.86CDD113 pKa = 3.8YY114 pKa = 11.07DD115 pKa = 3.71IEE117 pKa = 4.87NFWACAGASQSGEE130 pKa = 4.12EE131 pKa = 4.25FLHH134 pKa = 7.05FLDD137 pKa = 4.3QLAPRR142 pKa = 11.84DD143 pKa = 3.71LMRR146 pKa = 11.84GFIQFRR152 pKa = 11.84SYY154 pKa = 11.73ADD156 pKa = 3.42WKK158 pKa = 9.37WAVAPEE164 pKa = 4.48RR165 pKa = 11.84YY166 pKa = 9.68VNPPGVMFDD175 pKa = 3.49TGHH178 pKa = 7.26AEE180 pKa = 4.63QLSEE184 pKa = 4.08WLSQANLGSGPGRR197 pKa = 11.84VRR199 pKa = 11.84LVAHH203 pKa = 6.22LHH205 pKa = 5.95GSRR208 pKa = 11.84KK209 pKa = 9.99SLMLWGPTQYY219 pKa = 11.46GKK221 pKa = 6.97TTWARR226 pKa = 11.84SLGNHH231 pKa = 6.2IFFGSQFSGKK241 pKa = 9.72LALDD245 pKa = 4.14GMQDD249 pKa = 2.71AEE251 pKa = 4.41YY252 pKa = 10.96AVFDD256 pKa = 3.84DD257 pKa = 4.1WKK259 pKa = 10.9GGMKK263 pKa = 10.47ALPGYY268 pKa = 9.93KK269 pKa = 9.62DD270 pKa = 2.93WFGCQWQISVRR281 pKa = 11.84KK282 pKa = 8.73LHH284 pKa = 7.02HH285 pKa = 6.67DD286 pKa = 3.36AKK288 pKa = 10.63LITWGRR294 pKa = 11.84PIIWLCNKK302 pKa = 9.85DD303 pKa = 3.55PRR305 pKa = 11.84LMHH308 pKa = 6.42VATDD312 pKa = 3.78DD313 pKa = 4.83VDD315 pKa = 3.98WEE317 pKa = 4.22WMDD320 pKa = 4.21DD321 pKa = 3.37NVIFVEE327 pKa = 4.38LARR330 pKa = 11.84PLATFRR336 pKa = 11.84ASTEE340 pKa = 3.78

Molecular weight: 38.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UH04|A0A0B4UH04_9VIRU Replication-associated protein OS=Odonata associated gemycircularvirus-2 OX=1592108 PE=3 SV=1
MM1 pKa = 7.56VYY3 pKa = 10.08RR4 pKa = 11.84SKK6 pKa = 10.87SRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84TTRR13 pKa = 11.84RR14 pKa = 11.84SSTRR18 pKa = 11.84KK19 pKa = 9.28RR20 pKa = 11.84PSTRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84SYY28 pKa = 10.06GKK30 pKa = 9.72KK31 pKa = 9.44RR32 pKa = 11.84STFRR36 pKa = 11.84SRR38 pKa = 11.84RR39 pKa = 11.84MSSKK43 pKa = 10.59RR44 pKa = 11.84ILNLTSKK51 pKa = 10.45KK52 pKa = 10.44KK53 pKa = 9.48QDD55 pKa = 3.37NMLSFSNTASDD66 pKa = 3.9GTPTTVAQGSLYY78 pKa = 10.34VAGVQSGATSNYY90 pKa = 9.23GMSIFCPTARR100 pKa = 11.84SLVTAGPPISRR111 pKa = 11.84WMSQTEE117 pKa = 4.12PPPHH121 pKa = 5.12VTLRR125 pKa = 11.84GYY127 pKa = 10.55KK128 pKa = 9.86EE129 pKa = 3.74NLRR132 pKa = 11.84IQTSSSQPWLWRR144 pKa = 11.84RR145 pKa = 11.84IVFNTKK151 pKa = 10.0GPTFSSTSVGDD162 pKa = 3.18TATRR166 pKa = 11.84QKK168 pKa = 10.92YY169 pKa = 10.21SPFSDD174 pKa = 3.35TSIGMARR181 pKa = 11.84LWFNTSVNAMSATQSLFQTVMFKK204 pKa = 10.99GAINQDD210 pKa = 2.59WSDD213 pKa = 4.48PITAKK218 pKa = 10.45VDD220 pKa = 3.34TSRR223 pKa = 11.84ITVMSDD229 pKa = 2.6RR230 pKa = 11.84TQTIKK235 pKa = 9.22TQNSNGHH242 pKa = 4.81FSEE245 pKa = 4.42RR246 pKa = 11.84KK247 pKa = 8.63LWYY250 pKa = 9.36PMKK253 pKa = 10.69KK254 pKa = 10.08NLVYY258 pKa = 10.94DD259 pKa = 4.23DD260 pKa = 5.4DD261 pKa = 4.16EE262 pKa = 6.9AGAAEE267 pKa = 4.31VPAYY271 pKa = 10.51YY272 pKa = 9.39STDD275 pKa = 3.29AKK277 pKa = 10.74PGMGDD282 pKa = 3.26VYY284 pKa = 10.82IVDD287 pKa = 4.46FVMPSVGATASDD299 pKa = 3.91IINFNCTSTLYY310 pKa = 9.52WHH312 pKa = 7.05EE313 pKa = 4.2KK314 pKa = 9.32

Molecular weight: 35.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

654

314

340

327.0

36.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.339 ± 0.948

1.07 ± 0.319

6.575 ± 1.323

3.211 ± 0.957

5.046 ± 0.901

7.492 ± 1.529

2.599 ± 1.21

3.976 ± 0.121

5.046 ± 0.739

6.422 ± 1.679

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.211 ± 0.449

3.67 ± 0.58

4.74 ± 0.262

3.517 ± 0.224

7.034 ± 1.152

9.174 ± 3.091

7.492 ± 2.689

5.657 ± 0.055

3.364 ± 0.835

3.364 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski