Beihai sobemo-like virus 16

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE58|A0A1L3KE58_9VIRU RdRP_1 domain-containing protein OS=Beihai sobemo-like virus 16 OX=1922687 PE=4 SV=1
MM1 pKa = 7.4FRR3 pKa = 11.84PIMRR7 pKa = 11.84SVQADD12 pKa = 3.75CLSNGIAIGWAPNLPGGLRR31 pKa = 11.84TLWSHH36 pKa = 6.5IRR38 pKa = 11.84NKK40 pKa = 10.35DD41 pKa = 3.03LCTDD45 pKa = 3.4KK46 pKa = 11.43SSFDD50 pKa = 3.36WTVQPWMMEE59 pKa = 3.88FVAEE63 pKa = 4.42LFSSLVDD70 pKa = 3.61PANSTVVYY78 pKa = 8.16NHH80 pKa = 6.71CCSMLGAKK88 pKa = 8.64TFTAPGVSFHH98 pKa = 6.9HH99 pKa = 6.49PHH101 pKa = 7.42DD102 pKa = 4.41GVLPSGWKK110 pKa = 8.39LTILVNSILQLSFHH124 pKa = 6.62EE125 pKa = 4.17LAGGIGSIIAMGDD138 pKa = 3.18DD139 pKa = 4.53TIQDD143 pKa = 3.46RR144 pKa = 11.84VDD146 pKa = 3.16NTPAYY151 pKa = 9.56MEE153 pKa = 4.44ALSRR157 pKa = 11.84LGPLIKK163 pKa = 10.39VATIGHH169 pKa = 6.51EE170 pKa = 4.06FCGMEE175 pKa = 4.26FTAEE179 pKa = 4.0KK180 pKa = 10.13YY181 pKa = 10.85LPVYY185 pKa = 10.18LEE187 pKa = 3.83KK188 pKa = 10.6HH189 pKa = 5.91SYY191 pKa = 9.82ALRR194 pKa = 11.84HH195 pKa = 5.84LKK197 pKa = 10.74ASVAVEE203 pKa = 3.99TLASYY208 pKa = 10.64QYY210 pKa = 11.37LYY212 pKa = 10.79AYY214 pKa = 10.62DD215 pKa = 3.96NARR218 pKa = 11.84LAAIQDD224 pKa = 3.3WMRR227 pKa = 11.84QLGSPDD233 pKa = 3.21LVKK236 pKa = 10.59PRR238 pKa = 11.84LQLQFEE244 pKa = 4.31PRR246 pKa = 11.84GWSIPISYY254 pKa = 10.56

Molecular weight: 28.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE58|A0A1L3KE58_9VIRU RdRP_1 domain-containing protein OS=Beihai sobemo-like virus 16 OX=1922687 PE=4 SV=1
MM1 pKa = 7.45QLFTLSLGLTFSLWLLTRR19 pKa = 11.84TAAATLQNEE28 pKa = 4.54RR29 pKa = 11.84FYY31 pKa = 11.57SSTDD35 pKa = 3.19SFDD38 pKa = 3.32VDD40 pKa = 3.76PVMVAYY46 pKa = 8.26FHH48 pKa = 6.45YY49 pKa = 10.81AFLFFFLVTMIGMTVCFILCPIFLWISRR77 pKa = 11.84TIGYY81 pKa = 9.29CLLGTYY87 pKa = 10.55LPGFKK92 pKa = 10.61LEE94 pKa = 4.75LLNEE98 pKa = 3.85MSVRR102 pKa = 11.84EE103 pKa = 4.23KK104 pKa = 10.21KK105 pKa = 9.13TDD107 pKa = 4.26FIVGAPLPDD116 pKa = 3.69CQVIVYY122 pKa = 10.0RR123 pKa = 11.84RR124 pKa = 11.84LNDD127 pKa = 3.79DD128 pKa = 3.34YY129 pKa = 11.08VTAVGQGFFVRR140 pKa = 11.84DD141 pKa = 3.83GEE143 pKa = 4.74IVTAQHH149 pKa = 5.74NVEE152 pKa = 4.38TAGEE156 pKa = 4.38YY157 pKa = 10.35YY158 pKa = 9.97VAKK161 pKa = 10.47GHH163 pKa = 6.19TEE165 pKa = 4.02LKK167 pKa = 10.64FKK169 pKa = 10.14VTGDD173 pKa = 3.65RR174 pKa = 11.84EE175 pKa = 4.39LEE177 pKa = 4.32GPQIDD182 pKa = 4.34LVVLQVPKK190 pKa = 11.02GVMQKK195 pKa = 8.04MHH197 pKa = 7.42ASPAKK202 pKa = 9.66LQSRR206 pKa = 11.84VLNNQGITIHH216 pKa = 6.89AGGLRR221 pKa = 11.84DD222 pKa = 4.45DD223 pKa = 4.39DD224 pKa = 4.45TVLSSNGTIAAMSGRR239 pKa = 11.84EE240 pKa = 3.9GVLTCGHH247 pKa = 6.4TADD250 pKa = 4.42TLPGFSGAPIFLGRR264 pKa = 11.84KK265 pKa = 7.92VVAIHH270 pKa = 5.49MTSIPSKK277 pKa = 9.64VNSGSWIWPALGDD290 pKa = 3.67YY291 pKa = 7.45FHH293 pKa = 7.56PVLSRR298 pKa = 11.84RR299 pKa = 11.84EE300 pKa = 3.75TAAFSSSTSRR310 pKa = 11.84KK311 pKa = 10.06SSDD314 pKa = 2.27IDD316 pKa = 3.22QFMEE320 pKa = 3.95YY321 pKa = 10.7DD322 pKa = 3.34EE323 pKa = 5.31DD324 pKa = 4.49VYY326 pKa = 11.06IRR328 pKa = 11.84SRR330 pKa = 11.84SGQLRR335 pKa = 11.84KK336 pKa = 9.3MKK338 pKa = 10.74AEE340 pKa = 4.21DD341 pKa = 3.47FFEE344 pKa = 4.43YY345 pKa = 10.88AEE347 pKa = 4.16PTFLRR352 pKa = 11.84SYY354 pKa = 10.86EE355 pKa = 4.13EE356 pKa = 3.89NQEE359 pKa = 4.17LLEE362 pKa = 4.45QGASWAEE369 pKa = 3.69VSKK372 pKa = 10.31WADD375 pKa = 3.38EE376 pKa = 4.25DD377 pKa = 3.59QLAQRR382 pKa = 11.84EE383 pKa = 4.55TNKK386 pKa = 9.73TPVSDD391 pKa = 3.98TDD393 pKa = 4.09SDD395 pKa = 4.74DD396 pKa = 4.02SDD398 pKa = 3.87STSSFVSTKK407 pKa = 10.37SSSSSYY413 pKa = 10.33ASCRR417 pKa = 11.84PEE419 pKa = 3.58PLARR423 pKa = 11.84RR424 pKa = 11.84EE425 pKa = 3.99NLDD428 pKa = 4.56PIPLPTEE435 pKa = 3.7NLRR438 pKa = 11.84PQAVLDD444 pKa = 3.88AVTEE448 pKa = 3.9YY449 pKa = 11.15AIILRR454 pKa = 11.84RR455 pKa = 11.84YY456 pKa = 9.47NEE458 pKa = 4.47LKK460 pKa = 11.16VMIDD464 pKa = 3.47ILNHH468 pKa = 6.27ASNHH472 pKa = 5.18SLALRR477 pKa = 11.84DD478 pKa = 3.69QQSNFQLSSDD488 pKa = 2.99IDD490 pKa = 3.54VLRR493 pKa = 11.84RR494 pKa = 11.84EE495 pKa = 4.72LEE497 pKa = 3.92NASAVLKK504 pKa = 10.49EE505 pKa = 4.37SNLKK509 pKa = 10.3LSHH512 pKa = 6.73IMQKK516 pKa = 10.27SADD519 pKa = 3.92DD520 pKa = 3.84AQVARR525 pKa = 11.84NWQRR529 pKa = 11.84YY530 pKa = 5.18RR531 pKa = 11.84HH532 pKa = 5.1SHH534 pKa = 6.18ADD536 pKa = 3.01VRR538 pKa = 11.84PPVSQQTQDD547 pKa = 3.4LLAAARR553 pKa = 11.84MEE555 pKa = 4.16IDD557 pKa = 3.38AASQRR562 pKa = 11.84LQAIPEE568 pKa = 4.14RR569 pKa = 11.84PRR571 pKa = 11.84QEE573 pKa = 3.65PFAVRR578 pKa = 11.84EE579 pKa = 4.24SVEE582 pKa = 3.7ADD584 pKa = 3.17AVISRR589 pKa = 11.84YY590 pKa = 10.14LPGFFNRR597 pKa = 11.84GAHH600 pKa = 5.98PAGPSVSKK608 pKa = 10.48PSNQTIQAFLPEE620 pKa = 4.75KK621 pKa = 10.51DD622 pKa = 4.12VNPQGNKK629 pKa = 9.98LSSAQPNGSDD639 pKa = 3.53KK640 pKa = 11.15APPPTPDD647 pKa = 4.8DD648 pKa = 4.08SLLLPPPLKK657 pKa = 10.66DD658 pKa = 3.21SSPPTTPIRR667 pKa = 11.84STTVAPTTPSTAAKK681 pKa = 9.88SSPKK685 pKa = 9.48RR686 pKa = 11.84RR687 pKa = 11.84RR688 pKa = 11.84RR689 pKa = 11.84SRR691 pKa = 11.84NSSRR695 pKa = 11.84RR696 pKa = 11.84RR697 pKa = 11.84KK698 pKa = 6.19TTSNPQNPSTPQPSAPHH715 pKa = 5.7SASTRR720 pKa = 11.84EE721 pKa = 4.07SLVPPGVDD729 pKa = 2.52ATLQIEE735 pKa = 4.52KK736 pKa = 10.66LSLAQRR742 pKa = 11.84KK743 pKa = 9.0SLMKK747 pKa = 10.59DD748 pKa = 3.35LLRR751 pKa = 11.84DD752 pKa = 3.28PDD754 pKa = 3.83AYY756 pKa = 11.35LLVGPGEE763 pKa = 4.55NSPSSPPSSPSEE775 pKa = 4.01VSSPPLL781 pKa = 3.55

Molecular weight: 86.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1035

254

781

517.5

57.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.019 ± 0.115

1.063 ± 0.419

5.894 ± 0.359

5.024 ± 0.503

3.865 ± 0.033

5.121 ± 0.727

2.319 ± 0.567

4.444 ± 0.676

3.961 ± 0.193

9.855 ± 0.541

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.319 ± 0.567

3.478 ± 0.152

7.343 ± 0.665

4.444 ± 0.417

6.087 ± 0.813

10.531 ± 1.047

5.797 ± 0.496

6.184 ± 0.129

1.353 ± 0.649

2.899 ± 0.481
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski