Pseudomonas phage vB_PaeM_C2-10_Ab08

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pakpunavirus; Pseudomonas virus CAb1

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1IUF2|A0A0A1IUF2_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeM_C2-10_Ab08 OX=1548903 GN=ORF164 PE=4 SV=1
MM1 pKa = 7.62SILALTLTLCSAVQCDD17 pKa = 5.16DD18 pKa = 4.29YY19 pKa = 11.33IIDD22 pKa = 4.03HH23 pKa = 6.42SLPGQAAEE31 pKa = 4.28CNARR35 pKa = 11.84LVEE38 pKa = 4.31EE39 pKa = 4.3AEE41 pKa = 4.14EE42 pKa = 4.33WGDD45 pKa = 3.11AWVATNADD53 pKa = 3.58TRR55 pKa = 11.84LTRR58 pKa = 11.84YY59 pKa = 9.93LSRR62 pKa = 11.84FNIQVDD68 pKa = 3.36PRR70 pKa = 11.84FVFDD74 pKa = 5.54YY75 pKa = 11.21DD76 pKa = 3.86FTCQLIAEE84 pKa = 4.86DD85 pKa = 4.44EE86 pKa = 4.45LPP88 pKa = 4.02

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1IVK1|A0A0A1IVK1_9CAUD Putative Nicotinamide phosphoribosyltransferase OS=Pseudomonas phage vB_PaeM_C2-10_Ab08 OX=1548903 GN=ORF20 PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 10.22RR3 pKa = 11.84QYY5 pKa = 10.12TDD7 pKa = 3.11SYY9 pKa = 9.11QCKK12 pKa = 10.24AIDD15 pKa = 4.02GLMSICGGKK24 pKa = 9.6KK25 pKa = 10.25AIVQVWPSLKK35 pKa = 10.09ASRR38 pKa = 11.84NRR40 pKa = 11.84RR41 pKa = 11.84LKK43 pKa = 11.21KK44 pKa = 7.73MVKK47 pKa = 9.83YY48 pKa = 10.25IARR51 pKa = 11.84SGWKK55 pKa = 9.07PP56 pKa = 3.04

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

173

0

173

27582

37

904

159.4

18.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.045 ± 0.304

1.425 ± 0.109

6.138 ± 0.158

7.719 ± 0.238

4.162 ± 0.11

7.001 ± 0.199

1.922 ± 0.105

5.543 ± 0.146

6.254 ± 0.228

7.907 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.053 ± 0.108

4.213 ± 0.166

3.622 ± 0.127

3.502 ± 0.124

5.572 ± 0.158

5.63 ± 0.141

5.46 ± 0.206

7.218 ± 0.197

1.827 ± 0.093

3.785 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski