Tomato yellow leaf curl Kanchanaburi virus-[Thailand Kan1]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato yellow leaf curl Kanchanaburi virus

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8B5F6|Q8B5F6_9GEMI AC4 OS=Tomato yellow leaf curl Kanchanaburi virus-[Thailand Kan1] OX=266800 GN=AC4 PE=3 SV=1
MM1 pKa = 7.44EE2 pKa = 5.32NMWDD6 pKa = 3.8PLLHH10 pKa = 6.68PFPEE14 pKa = 4.42SLHH17 pKa = 6.17GFRR20 pKa = 11.84CMLAIKK26 pKa = 9.65YY27 pKa = 7.76LQSLQSKK34 pKa = 9.17YY35 pKa = 11.16SPDD38 pKa = 3.41TLGSEE43 pKa = 4.16FLKK46 pKa = 10.99DD47 pKa = 4.5FICILRR53 pKa = 11.84SRR55 pKa = 11.84NYY57 pKa = 9.99AEE59 pKa = 4.03AFNRR63 pKa = 11.84YY64 pKa = 8.87SDD66 pKa = 3.83VVANVYY72 pKa = 7.61NTPEE76 pKa = 3.83VKK78 pKa = 10.34LRR80 pKa = 11.84EE81 pKa = 4.44SVQSPCLCPHH91 pKa = 7.13CPRR94 pKa = 11.84HH95 pKa = 5.14VVQTKK100 pKa = 9.58SLEE103 pKa = 4.15KK104 pKa = 9.83PSYY107 pKa = 8.09VHH109 pKa = 6.57EE110 pKa = 4.29ATVLSVAKK118 pKa = 9.94EE119 pKa = 3.81QQ120 pKa = 3.64

Molecular weight: 13.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8B5G0|Q8B5G0_9GEMI Capsid protein OS=Tomato yellow leaf curl Kanchanaburi virus-[Thailand Kan1] OX=266800 GN=AV1 PE=3 SV=1
MM1 pKa = 7.94PKK3 pKa = 10.25RR4 pKa = 11.84SIDD7 pKa = 3.69TVTSLPMSITRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFGSQYY27 pKa = 10.73SLPASAPTAPGMSYY41 pKa = 10.55KK42 pKa = 10.11RR43 pKa = 11.84RR44 pKa = 11.84AWKK47 pKa = 9.56NRR49 pKa = 11.84PMYY52 pKa = 8.22TKK54 pKa = 9.96PRR56 pKa = 11.84FYY58 pKa = 10.51RR59 pKa = 11.84WRR61 pKa = 11.84RR62 pKa = 11.84SSDD65 pKa = 3.37VPRR68 pKa = 11.84GCEE71 pKa = 4.29GPCKK75 pKa = 10.21VQSFEE80 pKa = 3.81QRR82 pKa = 11.84HH83 pKa = 6.48DD84 pKa = 3.2ITHH87 pKa = 6.24TGKK90 pKa = 9.98VLCVSDD96 pKa = 3.48VTRR99 pKa = 11.84GNGITHH105 pKa = 7.65RR106 pKa = 11.84IGKK109 pKa = 8.43RR110 pKa = 11.84FCVKK114 pKa = 9.9SVYY117 pKa = 10.94VMGKK121 pKa = 8.03IWMDD125 pKa = 3.34EE126 pKa = 3.97NIKK129 pKa = 10.61LKK131 pKa = 10.74NHH133 pKa = 6.27TNTVMFWLVRR143 pKa = 11.84DD144 pKa = 3.99RR145 pKa = 11.84RR146 pKa = 11.84PVTTPYY152 pKa = 11.31GFGEE156 pKa = 4.7LFNMYY161 pKa = 10.54DD162 pKa = 4.09NEE164 pKa = 4.4PSTATIKK171 pKa = 10.86NDD173 pKa = 2.9LRR175 pKa = 11.84DD176 pKa = 3.56RR177 pKa = 11.84VQVLHH182 pKa = 6.82RR183 pKa = 11.84FSASLTGGQYY193 pKa = 10.84ASKK196 pKa = 9.35EE197 pKa = 3.64QAVIKK202 pKa = 10.76KK203 pKa = 7.91FFRR206 pKa = 11.84VNNYY210 pKa = 7.8VVYY213 pKa = 10.46NHH215 pKa = 6.03QEE217 pKa = 3.42AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 9.86ATLKK247 pKa = 9.47IRR249 pKa = 11.84IYY251 pKa = 10.67FYY253 pKa = 11.5DD254 pKa = 3.33NVTNN258 pKa = 4.07

Molecular weight: 30.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1650

100

349

206.3

23.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.909 ± 0.56

2.182 ± 0.234

4.424 ± 0.209

4.667 ± 0.586

4.485 ± 0.623

4.606 ± 0.497

3.455 ± 0.206

5.152 ± 0.627

6.242 ± 0.536

7.152 ± 0.721

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.337

6.788 ± 0.59

5.333 ± 0.339

5.03 ± 0.698

5.939 ± 0.975

9.091 ± 0.967

5.152 ± 0.468

7.03 ± 0.858

1.515 ± 0.212

4.424 ± 0.52
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski