Amino acid dipepetide frequency for Arthrobacter phage GurgleFerb

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.194AlaAla: 7.194 ± 0.796
0.36AlaCys: 0.36 ± 0.137
4.316AlaAsp: 4.316 ± 0.541
5.251AlaGlu: 5.251 ± 0.594
2.374AlaPhe: 2.374 ± 0.338
6.546AlaGly: 6.546 ± 0.746
2.23AlaHis: 2.23 ± 0.451
4.388AlaIle: 4.388 ± 0.643
5.827AlaLys: 5.827 ± 0.943
6.906AlaLeu: 6.906 ± 0.678
2.734AlaMet: 2.734 ± 0.4
5.539AlaAsn: 5.539 ± 0.74
3.741AlaPro: 3.741 ± 0.614
4.1AlaGln: 4.1 ± 0.717
3.669AlaArg: 3.669 ± 0.559
4.244AlaSer: 4.244 ± 0.49
5.108AlaThr: 5.108 ± 0.67
5.179AlaVal: 5.179 ± 0.519
1.007AlaTrp: 1.007 ± 0.233
3.237AlaTyr: 3.237 ± 0.408
0.0AlaXaa: 0.0 ± 0.0
Cys
0.432CysAla: 0.432 ± 0.17
0.144CysCys: 0.144 ± 0.096
0.216CysAsp: 0.216 ± 0.134
0.504CysGlu: 0.504 ± 0.167
0.0CysPhe: 0.0 ± 0.0
0.432CysGly: 0.432 ± 0.181
0.144CysHis: 0.144 ± 0.114
0.144CysIle: 0.144 ± 0.098
0.36CysLys: 0.36 ± 0.143
0.432CysLeu: 0.432 ± 0.198
0.288CysMet: 0.288 ± 0.114
0.216CysAsn: 0.216 ± 0.162
0.216CysPro: 0.216 ± 0.113
0.144CysGln: 0.144 ± 0.096
0.575CysArg: 0.575 ± 0.17
0.504CysSer: 0.504 ± 0.256
0.432CysThr: 0.432 ± 0.154
0.432CysVal: 0.432 ± 0.176
0.072CysTrp: 0.072 ± 0.067
0.072CysTyr: 0.072 ± 0.065
0.0CysXaa: 0.0 ± 0.0
Asp
4.532AspAla: 4.532 ± 0.466
0.0AspCys: 0.0 ± 0.0
3.237AspAsp: 3.237 ± 0.541
4.316AspGlu: 4.316 ± 0.615
3.165AspPhe: 3.165 ± 0.454
5.108AspGly: 5.108 ± 0.624
0.719AspHis: 0.719 ± 0.231
4.244AspIle: 4.244 ± 0.423
4.028AspLys: 4.028 ± 0.602
4.964AspLeu: 4.964 ± 0.617
2.302AspMet: 2.302 ± 0.384
3.237AspAsn: 3.237 ± 0.494
3.597AspPro: 3.597 ± 0.542
2.23AspGln: 2.23 ± 0.458
2.734AspArg: 2.734 ± 0.473
3.453AspSer: 3.453 ± 0.595
2.59AspThr: 2.59 ± 0.35
4.46AspVal: 4.46 ± 0.5
0.935AspTrp: 0.935 ± 0.233
1.726AspTyr: 1.726 ± 0.255
0.0AspXaa: 0.0 ± 0.0
Glu
6.546GluAla: 6.546 ± 0.903
0.647GluCys: 0.647 ± 0.221
4.172GluAsp: 4.172 ± 0.493
5.971GluGlu: 5.971 ± 0.837
3.741GluPhe: 3.741 ± 0.426
3.453GluGly: 3.453 ± 0.487
1.079GluHis: 1.079 ± 0.278
4.388GluIle: 4.388 ± 0.663
4.82GluLys: 4.82 ± 0.626
4.748GluLeu: 4.748 ± 0.637
2.518GluMet: 2.518 ± 0.466
4.676GluAsn: 4.676 ± 0.586
2.23GluPro: 2.23 ± 0.475
3.237GluGln: 3.237 ± 0.482
2.806GluArg: 2.806 ± 0.44
4.964GluSer: 4.964 ± 0.807
4.244GluThr: 4.244 ± 0.608
4.244GluVal: 4.244 ± 0.573
0.863GluTrp: 0.863 ± 0.221
2.374GluTyr: 2.374 ± 0.474
0.0GluXaa: 0.0 ± 0.0
Phe
2.59PheAla: 2.59 ± 0.468
0.216PheCys: 0.216 ± 0.121
3.597PheAsp: 3.597 ± 0.492
3.021PheGlu: 3.021 ± 0.357
1.655PhePhe: 1.655 ± 0.29
2.662PheGly: 2.662 ± 0.507
1.511PheHis: 1.511 ± 0.336
2.374PheIle: 2.374 ± 0.409
2.23PheLys: 2.23 ± 0.38
2.662PheLeu: 2.662 ± 0.44
0.863PheMet: 0.863 ± 0.24
2.446PheAsn: 2.446 ± 0.397
1.439PhePro: 1.439 ± 0.329
1.151PheGln: 1.151 ± 0.278
1.798PheArg: 1.798 ± 0.3
2.877PheSer: 2.877 ± 0.476
2.23PheThr: 2.23 ± 0.392
2.518PheVal: 2.518 ± 0.45
0.288PheTrp: 0.288 ± 0.141
1.079PheTyr: 1.079 ± 0.245
0.0PheXaa: 0.0 ± 0.0
Gly
4.82GlyAla: 4.82 ± 0.652
0.144GlyCys: 0.144 ± 0.116
3.885GlyAsp: 3.885 ± 0.728
3.525GlyGlu: 3.525 ± 0.354
3.885GlyPhe: 3.885 ± 0.458
6.834GlyGly: 6.834 ± 0.687
0.791GlyHis: 0.791 ± 0.231
4.532GlyIle: 4.532 ± 0.68
5.539GlyLys: 5.539 ± 0.812
5.108GlyLeu: 5.108 ± 0.523
2.446GlyMet: 2.446 ± 0.398
3.669GlyAsn: 3.669 ± 0.436
2.086GlyPro: 2.086 ± 0.385
2.446GlyGln: 2.446 ± 0.476
3.237GlyArg: 3.237 ± 0.557
6.043GlySer: 6.043 ± 0.862
5.251GlyThr: 5.251 ± 0.702
4.82GlyVal: 4.82 ± 0.458
1.511GlyTrp: 1.511 ± 0.274
2.374GlyTyr: 2.374 ± 0.46
0.0GlyXaa: 0.0 ± 0.0
His
1.079HisAla: 1.079 ± 0.273
0.0HisCys: 0.0 ± 0.0
1.151HisAsp: 1.151 ± 0.249
1.007HisGlu: 1.007 ± 0.217
0.791HisPhe: 0.791 ± 0.203
1.511HisGly: 1.511 ± 0.297
0.719HisHis: 0.719 ± 0.201
1.007HisIle: 1.007 ± 0.27
2.014HisLys: 2.014 ± 0.267
1.079HisLeu: 1.079 ± 0.325
0.791HisMet: 0.791 ± 0.212
1.223HisAsn: 1.223 ± 0.318
0.719HisPro: 0.719 ± 0.217
0.647HisGln: 0.647 ± 0.169
1.223HisArg: 1.223 ± 0.292
0.863HisSer: 0.863 ± 0.271
0.863HisThr: 0.863 ± 0.322
1.367HisVal: 1.367 ± 0.332
0.36HisTrp: 0.36 ± 0.175
0.36HisTyr: 0.36 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
5.611IleAla: 5.611 ± 0.704
0.647IleCys: 0.647 ± 0.243
3.237IleAsp: 3.237 ± 0.452
4.244IleGlu: 4.244 ± 0.573
2.086IlePhe: 2.086 ± 0.392
3.237IleGly: 3.237 ± 0.493
1.151IleHis: 1.151 ± 0.24
2.374IleIle: 2.374 ± 0.42
3.021IleLys: 3.021 ± 0.542
3.957IleLeu: 3.957 ± 0.433
1.223IleMet: 1.223 ± 0.291
3.237IleAsn: 3.237 ± 0.392
2.158IlePro: 2.158 ± 0.389
3.309IleGln: 3.309 ± 0.426
3.021IleArg: 3.021 ± 0.433
3.813IleSer: 3.813 ± 0.542
3.309IleThr: 3.309 ± 0.537
2.446IleVal: 2.446 ± 0.382
0.647IleTrp: 0.647 ± 0.206
1.223IleTyr: 1.223 ± 0.325
0.0IleXaa: 0.0 ± 0.0
Lys
6.115LysAla: 6.115 ± 0.847
0.36LysCys: 0.36 ± 0.172
3.741LysAsp: 3.741 ± 0.574
5.611LysGlu: 5.611 ± 0.765
2.374LysPhe: 2.374 ± 0.351
2.949LysGly: 2.949 ± 0.577
0.647LysHis: 0.647 ± 0.196
3.093LysIle: 3.093 ± 0.333
4.1LysLys: 4.1 ± 0.641
4.892LysLeu: 4.892 ± 0.766
1.726LysMet: 1.726 ± 0.349
3.381LysAsn: 3.381 ± 0.4
3.957LysPro: 3.957 ± 0.684
2.806LysGln: 2.806 ± 0.398
2.877LysArg: 2.877 ± 0.526
3.597LysSer: 3.597 ± 0.511
4.1LysThr: 4.1 ± 0.531
4.028LysVal: 4.028 ± 0.52
0.935LysTrp: 0.935 ± 0.218
2.59LysTyr: 2.59 ± 0.356
0.0LysXaa: 0.0 ± 0.0
Leu
8.561LeuAla: 8.561 ± 0.707
0.216LeuCys: 0.216 ± 0.136
6.043LeuAsp: 6.043 ± 0.677
5.108LeuGlu: 5.108 ± 0.685
2.23LeuPhe: 2.23 ± 0.425
5.683LeuGly: 5.683 ± 0.51
1.079LeuHis: 1.079 ± 0.201
4.028LeuIle: 4.028 ± 0.554
4.028LeuLys: 4.028 ± 0.482
4.82LeuLeu: 4.82 ± 0.601
1.726LeuMet: 1.726 ± 0.379
3.597LeuAsn: 3.597 ± 0.447
3.741LeuPro: 3.741 ± 0.555
3.381LeuGln: 3.381 ± 0.566
3.093LeuArg: 3.093 ± 0.419
5.179LeuSer: 5.179 ± 0.632
3.669LeuThr: 3.669 ± 0.638
4.028LeuVal: 4.028 ± 0.481
1.223LeuTrp: 1.223 ± 0.234
2.014LeuTyr: 2.014 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
3.021MetAla: 3.021 ± 0.339
0.072MetCys: 0.072 ± 0.065
1.007MetAsp: 1.007 ± 0.229
1.942MetGlu: 1.942 ± 0.354
0.935MetPhe: 0.935 ± 0.222
2.374MetGly: 2.374 ± 0.328
0.719MetHis: 0.719 ± 0.192
1.007MetIle: 1.007 ± 0.247
2.59MetLys: 2.59 ± 0.399
2.086MetLeu: 2.086 ± 0.483
0.504MetMet: 0.504 ± 0.22
1.079MetAsn: 1.079 ± 0.29
1.511MetPro: 1.511 ± 0.29
0.935MetGln: 0.935 ± 0.191
1.439MetArg: 1.439 ± 0.222
2.158MetSer: 2.158 ± 0.366
1.726MetThr: 1.726 ± 0.214
2.23MetVal: 2.23 ± 0.369
0.432MetTrp: 0.432 ± 0.155
0.935MetTyr: 0.935 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
4.676AsnAla: 4.676 ± 0.433
0.504AsnCys: 0.504 ± 0.196
3.741AsnAsp: 3.741 ± 0.434
4.244AsnGlu: 4.244 ± 0.597
2.014AsnPhe: 2.014 ± 0.36
4.532AsnGly: 4.532 ± 0.563
0.719AsnHis: 0.719 ± 0.153
2.806AsnIle: 2.806 ± 0.466
3.237AsnLys: 3.237 ± 0.483
3.813AsnLeu: 3.813 ± 0.459
1.798AsnMet: 1.798 ± 0.329
2.806AsnAsn: 2.806 ± 0.58
3.309AsnPro: 3.309 ± 0.43
1.942AsnGln: 1.942 ± 0.404
2.518AsnArg: 2.518 ± 0.407
2.806AsnSer: 2.806 ± 0.592
2.949AsnThr: 2.949 ± 0.528
3.093AsnVal: 3.093 ± 0.379
0.504AsnTrp: 0.504 ± 0.219
1.87AsnTyr: 1.87 ± 0.504
0.0AsnXaa: 0.0 ± 0.0
Pro
3.093ProAla: 3.093 ± 0.544
0.072ProCys: 0.072 ± 0.07
3.309ProAsp: 3.309 ± 0.583
4.748ProGlu: 4.748 ± 0.663
2.086ProPhe: 2.086 ± 0.325
2.59ProGly: 2.59 ± 0.43
0.647ProHis: 0.647 ± 0.279
1.942ProIle: 1.942 ± 0.328
3.093ProLys: 3.093 ± 0.483
2.806ProLeu: 2.806 ± 0.481
1.079ProMet: 1.079 ± 0.321
2.446ProAsn: 2.446 ± 0.391
2.014ProPro: 2.014 ± 0.762
2.23ProGln: 2.23 ± 0.279
1.583ProArg: 1.583 ± 0.259
3.165ProSer: 3.165 ± 0.424
3.381ProThr: 3.381 ± 0.486
3.813ProVal: 3.813 ± 0.572
0.647ProTrp: 0.647 ± 0.228
0.935ProTyr: 0.935 ± 0.248
0.0ProXaa: 0.0 ± 0.0
Gln
3.813GlnAla: 3.813 ± 0.574
0.144GlnCys: 0.144 ± 0.106
2.374GlnAsp: 2.374 ± 0.388
2.949GlnGlu: 2.949 ± 0.514
1.655GlnPhe: 1.655 ± 0.299
2.806GlnGly: 2.806 ± 0.355
0.647GlnHis: 0.647 ± 0.226
2.158GlnIle: 2.158 ± 0.475
3.309GlnLys: 3.309 ± 0.418
3.813GlnLeu: 3.813 ± 0.531
1.151GlnMet: 1.151 ± 0.225
2.446GlnAsn: 2.446 ± 0.579
1.439GlnPro: 1.439 ± 0.33
2.59GlnGln: 2.59 ± 0.921
2.59GlnArg: 2.59 ± 0.462
3.165GlnSer: 3.165 ± 0.477
2.446GlnThr: 2.446 ± 0.373
2.662GlnVal: 2.662 ± 0.523
0.791GlnTrp: 0.791 ± 0.259
1.367GlnTyr: 1.367 ± 0.284
0.0GlnXaa: 0.0 ± 0.0
Arg
3.309ArgAla: 3.309 ± 0.412
0.432ArgCys: 0.432 ± 0.228
2.59ArgAsp: 2.59 ± 0.396
3.237ArgGlu: 3.237 ± 0.554
1.942ArgPhe: 1.942 ± 0.398
2.734ArgGly: 2.734 ± 0.462
1.511ArgHis: 1.511 ± 0.257
3.237ArgIle: 3.237 ± 0.439
1.942ArgLys: 1.942 ± 0.401
3.669ArgLeu: 3.669 ± 0.511
1.439ArgMet: 1.439 ± 0.292
2.446ArgAsn: 2.446 ± 0.499
1.655ArgPro: 1.655 ± 0.286
2.446ArgGln: 2.446 ± 0.399
2.518ArgArg: 2.518 ± 0.488
2.302ArgSer: 2.302 ± 0.403
3.237ArgThr: 3.237 ± 0.501
2.662ArgVal: 2.662 ± 0.499
0.791ArgTrp: 0.791 ± 0.265
2.302ArgTyr: 2.302 ± 0.403
0.0ArgXaa: 0.0 ± 0.0
Ser
5.036SerAla: 5.036 ± 0.548
0.36SerCys: 0.36 ± 0.192
3.741SerAsp: 3.741 ± 0.61
4.892SerGlu: 4.892 ± 0.458
1.583SerPhe: 1.583 ± 0.356
4.964SerGly: 4.964 ± 0.521
0.863SerHis: 0.863 ± 0.247
3.669SerIle: 3.669 ± 0.516
3.597SerLys: 3.597 ± 0.491
5.971SerLeu: 5.971 ± 0.816
1.798SerMet: 1.798 ± 0.369
2.949SerAsn: 2.949 ± 0.669
3.237SerPro: 3.237 ± 0.532
2.949SerGln: 2.949 ± 0.567
2.734SerArg: 2.734 ± 0.431
3.957SerSer: 3.957 ± 0.563
4.028SerThr: 4.028 ± 0.739
4.244SerVal: 4.244 ± 0.555
0.935SerTrp: 0.935 ± 0.261
2.302SerTyr: 2.302 ± 0.661
0.0SerXaa: 0.0 ± 0.0
Thr
4.964ThrAla: 4.964 ± 0.667
0.144ThrCys: 0.144 ± 0.09
3.453ThrAsp: 3.453 ± 0.652
3.165ThrGlu: 3.165 ± 0.518
1.942ThrPhe: 1.942 ± 0.288
5.899ThrGly: 5.899 ± 0.64
1.079ThrHis: 1.079 ± 0.237
3.093ThrIle: 3.093 ± 0.379
3.021ThrLys: 3.021 ± 0.447
4.172ThrLeu: 4.172 ± 0.638
1.655ThrMet: 1.655 ± 0.345
3.237ThrAsn: 3.237 ± 0.561
3.453ThrPro: 3.453 ± 0.436
2.806ThrGln: 2.806 ± 0.576
2.518ThrArg: 2.518 ± 0.418
3.741ThrSer: 3.741 ± 0.602
4.244ThrThr: 4.244 ± 0.697
4.388ThrVal: 4.388 ± 0.799
1.007ThrTrp: 1.007 ± 0.227
2.302ThrTyr: 2.302 ± 0.443
0.0ThrXaa: 0.0 ± 0.0
Val
4.964ValAla: 4.964 ± 0.64
0.575ValCys: 0.575 ± 0.174
4.748ValAsp: 4.748 ± 0.525
4.604ValGlu: 4.604 ± 0.657
2.518ValPhe: 2.518 ± 0.438
5.323ValGly: 5.323 ± 0.638
1.583ValHis: 1.583 ± 0.36
3.237ValIle: 3.237 ± 0.456
3.813ValLys: 3.813 ± 0.389
3.021ValLeu: 3.021 ± 0.607
1.655ValMet: 1.655 ± 0.416
2.374ValAsn: 2.374 ± 0.409
2.949ValPro: 2.949 ± 0.538
2.662ValGln: 2.662 ± 0.379
3.525ValArg: 3.525 ± 0.474
4.604ValSer: 4.604 ± 0.55
4.028ValThr: 4.028 ± 0.573
4.532ValVal: 4.532 ± 0.64
0.719ValTrp: 0.719 ± 0.209
2.302ValTyr: 2.302 ± 0.51
0.0ValXaa: 0.0 ± 0.0
Trp
0.935TrpAla: 0.935 ± 0.251
0.144TrpCys: 0.144 ± 0.099
0.935TrpAsp: 0.935 ± 0.345
1.079TrpGlu: 1.079 ± 0.273
0.36TrpPhe: 0.36 ± 0.14
1.079TrpGly: 1.079 ± 0.315
0.504TrpHis: 0.504 ± 0.209
0.935TrpIle: 0.935 ± 0.256
1.079TrpLys: 1.079 ± 0.264
1.151TrpLeu: 1.151 ± 0.214
0.144TrpMet: 0.144 ± 0.092
1.079TrpAsn: 1.079 ± 0.326
0.575TrpPro: 0.575 ± 0.168
0.719TrpGln: 0.719 ± 0.232
0.647TrpArg: 0.647 ± 0.251
0.719TrpSer: 0.719 ± 0.263
0.863TrpThr: 0.863 ± 0.216
0.719TrpVal: 0.719 ± 0.2
0.216TrpTrp: 0.216 ± 0.126
0.575TrpTyr: 0.575 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.734TyrAla: 2.734 ± 0.426
0.575TyrCys: 0.575 ± 0.207
2.086TyrAsp: 2.086 ± 0.448
2.302TyrGlu: 2.302 ± 0.479
1.87TyrPhe: 1.87 ± 0.44
2.23TyrGly: 2.23 ± 0.403
0.432TyrHis: 0.432 ± 0.161
1.295TyrIle: 1.295 ± 0.358
2.23TyrLys: 2.23 ± 0.337
3.381TyrLeu: 3.381 ± 0.544
0.791TyrMet: 0.791 ± 0.206
1.798TyrAsn: 1.798 ± 0.378
1.655TyrPro: 1.655 ± 0.413
1.583TyrGln: 1.583 ± 0.269
1.295TyrArg: 1.295 ± 0.273
1.655TyrSer: 1.655 ± 0.438
1.583TyrThr: 1.583 ± 0.265
1.87TyrVal: 1.87 ± 0.421
0.575TyrTrp: 0.575 ± 0.195
1.583TyrTyr: 1.583 ± 0.317
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13902 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski