Amino acid dipepetide frequency for Pseudomonas phage phCDa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.838AlaAla: 13.838 ± 1.991
0.901AlaCys: 0.901 ± 0.246
5.814AlaAsp: 5.814 ± 0.843
7.662AlaGlu: 7.662 ± 0.844
3.786AlaPhe: 3.786 ± 0.418
7.933AlaGly: 7.933 ± 0.704
1.713AlaHis: 1.713 ± 0.304
4.372AlaIle: 4.372 ± 0.507
5.589AlaLys: 5.589 ± 0.7
9.646AlaLeu: 9.646 ± 0.596
2.885AlaMet: 2.885 ± 0.399
4.372AlaAsn: 4.372 ± 0.522
3.696AlaPro: 3.696 ± 0.481
5.679AlaGln: 5.679 ± 0.898
4.778AlaArg: 4.778 ± 0.505
5.454AlaSer: 5.454 ± 0.845
5.995AlaThr: 5.995 ± 0.712
8.023AlaVal: 8.023 ± 0.721
1.532AlaTrp: 1.532 ± 0.267
2.749AlaTyr: 2.749 ± 0.331
0.0AlaXaa: 0.0 ± 0.0
Cys
0.676CysAla: 0.676 ± 0.186
0.18CysCys: 0.18 ± 0.115
0.451CysAsp: 0.451 ± 0.156
0.541CysGlu: 0.541 ± 0.178
0.18CysPhe: 0.18 ± 0.096
0.586CysGly: 0.586 ± 0.202
0.135CysHis: 0.135 ± 0.08
0.541CysIle: 0.541 ± 0.149
0.586CysLys: 0.586 ± 0.19
0.676CysLeu: 0.676 ± 0.216
0.361CysMet: 0.361 ± 0.127
0.361CysAsn: 0.361 ± 0.158
0.316CysPro: 0.316 ± 0.113
0.316CysGln: 0.316 ± 0.121
0.631CysArg: 0.631 ± 0.231
0.586CysSer: 0.586 ± 0.197
0.631CysThr: 0.631 ± 0.178
0.856CysVal: 0.856 ± 0.235
0.135CysTrp: 0.135 ± 0.089
0.541CysTyr: 0.541 ± 0.215
0.0CysXaa: 0.0 ± 0.0
Asp
5.409AspAla: 5.409 ± 0.723
0.586AspCys: 0.586 ± 0.231
3.516AspAsp: 3.516 ± 0.586
3.11AspGlu: 3.11 ± 0.504
2.254AspPhe: 2.254 ± 0.345
4.688AspGly: 4.688 ± 0.493
1.037AspHis: 1.037 ± 0.202
3.245AspIle: 3.245 ± 0.403
3.29AspLys: 3.29 ± 0.482
5.409AspLeu: 5.409 ± 0.45
1.758AspMet: 1.758 ± 0.342
2.344AspAsn: 2.344 ± 0.352
2.975AspPro: 2.975 ± 0.372
2.164AspGln: 2.164 ± 0.298
2.209AspArg: 2.209 ± 0.303
3.696AspSer: 3.696 ± 0.468
2.975AspThr: 2.975 ± 0.352
4.688AspVal: 4.688 ± 0.539
0.766AspTrp: 0.766 ± 0.197
1.578AspTyr: 1.578 ± 0.249
0.0AspXaa: 0.0 ± 0.0
Glu
7.077GluAla: 7.077 ± 0.714
0.856GluCys: 0.856 ± 0.245
2.885GluAsp: 2.885 ± 0.335
3.426GluGlu: 3.426 ± 0.56
2.344GluPhe: 2.344 ± 0.491
3.426GluGly: 3.426 ± 0.402
1.532GluHis: 1.532 ± 0.32
3.921GluIle: 3.921 ± 0.436
2.93GluLys: 2.93 ± 0.379
6.671GluLeu: 6.671 ± 0.654
2.209GluMet: 2.209 ± 0.264
2.659GluAsn: 2.659 ± 0.277
2.028GluPro: 2.028 ± 0.409
3.831GluGln: 3.831 ± 0.447
2.749GluArg: 2.749 ± 0.491
3.921GluSer: 3.921 ± 0.398
3.921GluThr: 3.921 ± 0.54
5.409GluVal: 5.409 ± 0.543
0.721GluTrp: 0.721 ± 0.176
1.848GluTyr: 1.848 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
3.245PheAla: 3.245 ± 0.313
0.27PheCys: 0.27 ± 0.136
2.84PheAsp: 2.84 ± 0.353
1.848PheGlu: 1.848 ± 0.275
1.352PhePhe: 1.352 ± 0.231
1.848PheGly: 1.848 ± 0.346
0.676PheHis: 0.676 ± 0.188
2.254PheIle: 2.254 ± 0.333
2.254PheLys: 2.254 ± 0.249
2.885PheLeu: 2.885 ± 0.364
1.397PheMet: 1.397 ± 0.357
2.524PheAsn: 2.524 ± 0.324
1.217PhePro: 1.217 ± 0.238
1.623PheGln: 1.623 ± 0.296
1.397PheArg: 1.397 ± 0.322
1.938PheSer: 1.938 ± 0.271
2.569PheThr: 2.569 ± 0.342
1.983PheVal: 1.983 ± 0.41
0.316PheTrp: 0.316 ± 0.136
1.037PheTyr: 1.037 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
5.679GlyAla: 5.679 ± 0.463
0.541GlyCys: 0.541 ± 0.185
3.381GlyAsp: 3.381 ± 0.354
3.876GlyGlu: 3.876 ± 0.43
2.795GlyPhe: 2.795 ± 0.326
3.876GlyGly: 3.876 ± 0.529
0.856GlyHis: 0.856 ± 0.171
4.012GlyIle: 4.012 ± 0.474
4.102GlyLys: 4.102 ± 0.384
6.22GlyLeu: 6.22 ± 0.559
2.118GlyMet: 2.118 ± 0.257
4.372GlyAsn: 4.372 ± 0.438
2.073GlyPro: 2.073 ± 0.323
3.155GlyGln: 3.155 ± 0.42
2.84GlyArg: 2.84 ± 0.344
4.462GlySer: 4.462 ± 0.362
4.372GlyThr: 4.372 ± 0.459
5.454GlyVal: 5.454 ± 0.488
1.487GlyTrp: 1.487 ± 0.283
2.885GlyTyr: 2.885 ± 0.367
0.0GlyXaa: 0.0 ± 0.0
His
1.397HisAla: 1.397 ± 0.257
0.225HisCys: 0.225 ± 0.102
1.487HisAsp: 1.487 ± 0.304
1.397HisGlu: 1.397 ± 0.28
0.676HisPhe: 0.676 ± 0.191
0.947HisGly: 0.947 ± 0.227
0.586HisHis: 0.586 ± 0.162
0.811HisIle: 0.811 ± 0.215
0.992HisLys: 0.992 ± 0.22
1.532HisLeu: 1.532 ± 0.325
0.947HisMet: 0.947 ± 0.199
0.811HisAsn: 0.811 ± 0.206
0.721HisPro: 0.721 ± 0.217
0.947HisGln: 0.947 ± 0.201
1.172HisArg: 1.172 ± 0.27
0.811HisSer: 0.811 ± 0.2
0.811HisThr: 0.811 ± 0.204
1.172HisVal: 1.172 ± 0.182
0.451HisTrp: 0.451 ± 0.18
0.361HisTyr: 0.361 ± 0.162
0.0HisXaa: 0.0 ± 0.0
Ile
5.544IleAla: 5.544 ± 0.553
0.316IleCys: 0.316 ± 0.131
3.741IleAsp: 3.741 ± 0.401
3.29IleGlu: 3.29 ± 0.4
1.127IlePhe: 1.127 ± 0.252
3.606IleGly: 3.606 ± 0.511
1.127IleHis: 1.127 ± 0.181
2.569IleIle: 2.569 ± 0.413
2.93IleLys: 2.93 ± 0.397
3.245IleLeu: 3.245 ± 0.469
1.623IleMet: 1.623 ± 0.261
1.938IleAsn: 1.938 ± 0.31
2.434IlePro: 2.434 ± 0.324
1.983IleGln: 1.983 ± 0.251
3.245IleArg: 3.245 ± 0.387
2.209IleSer: 2.209 ± 0.305
2.749IleThr: 2.749 ± 0.355
3.561IleVal: 3.561 ± 0.506
0.361IleTrp: 0.361 ± 0.146
1.442IleTyr: 1.442 ± 0.279
0.0IleXaa: 0.0 ± 0.0
Lys
5.679LysAla: 5.679 ± 0.739
0.225LysCys: 0.225 ± 0.134
2.84LysAsp: 2.84 ± 0.286
3.741LysGlu: 3.741 ± 0.443
2.164LysPhe: 2.164 ± 0.35
3.741LysGly: 3.741 ± 0.38
0.811LysHis: 0.811 ± 0.233
2.975LysIle: 2.975 ± 0.401
3.651LysLys: 3.651 ± 0.5
5.093LysLeu: 5.093 ± 0.474
1.397LysMet: 1.397 ± 0.291
2.479LysAsn: 2.479 ± 0.335
2.389LysPro: 2.389 ± 0.323
2.344LysGln: 2.344 ± 0.309
2.434LysArg: 2.434 ± 0.302
2.209LysSer: 2.209 ± 0.298
3.2LysThr: 3.2 ± 0.384
3.426LysVal: 3.426 ± 0.34
0.631LysTrp: 0.631 ± 0.189
1.713LysTyr: 1.713 ± 0.315
0.0LysXaa: 0.0 ± 0.0
Leu
9.06LeuAla: 9.06 ± 0.743
0.992LeuCys: 0.992 ± 0.249
5.634LeuAsp: 5.634 ± 0.593
5.454LeuGlu: 5.454 ± 0.458
3.471LeuPhe: 3.471 ± 0.532
6.175LeuGly: 6.175 ± 0.503
1.803LeuHis: 1.803 ± 0.269
4.507LeuIle: 4.507 ± 0.612
4.417LeuLys: 4.417 ± 0.462
7.572LeuLeu: 7.572 ± 0.712
3.29LeuMet: 3.29 ± 0.392
3.696LeuAsn: 3.696 ± 0.325
4.012LeuPro: 4.012 ± 0.456
4.372LeuGln: 4.372 ± 0.509
4.552LeuArg: 4.552 ± 0.426
5.905LeuSer: 5.905 ± 0.479
6.671LeuThr: 6.671 ± 0.563
5.995LeuVal: 5.995 ± 0.542
0.766LeuTrp: 0.766 ± 0.211
1.893LeuTyr: 1.893 ± 0.312
0.0LeuXaa: 0.0 ± 0.0
Met
3.335MetAla: 3.335 ± 0.403
0.406MetCys: 0.406 ± 0.124
1.623MetAsp: 1.623 ± 0.274
1.803MetGlu: 1.803 ± 0.327
0.766MetPhe: 0.766 ± 0.187
1.983MetGly: 1.983 ± 0.268
0.811MetHis: 0.811 ± 0.225
1.262MetIle: 1.262 ± 0.26
1.442MetLys: 1.442 ± 0.382
3.065MetLeu: 3.065 ± 0.378
0.811MetMet: 0.811 ± 0.158
1.352MetAsn: 1.352 ± 0.252
1.397MetPro: 1.397 ± 0.272
1.848MetGln: 1.848 ± 0.282
1.487MetArg: 1.487 ± 0.253
2.704MetSer: 2.704 ± 0.411
2.209MetThr: 2.209 ± 0.292
1.803MetVal: 1.803 ± 0.274
0.406MetTrp: 0.406 ± 0.131
0.947MetTyr: 0.947 ± 0.203
0.0MetXaa: 0.0 ± 0.0
Asn
5.589AsnAla: 5.589 ± 0.856
0.541AsnCys: 0.541 ± 0.15
2.118AsnAsp: 2.118 ± 0.295
2.975AsnGlu: 2.975 ± 0.349
1.758AsnPhe: 1.758 ± 0.278
3.471AsnGly: 3.471 ± 0.5
0.721AsnHis: 0.721 ± 0.183
2.073AsnIle: 2.073 ± 0.335
1.938AsnLys: 1.938 ± 0.297
4.057AsnLeu: 4.057 ± 0.396
1.307AsnMet: 1.307 ± 0.269
1.938AsnAsn: 1.938 ± 0.306
2.164AsnPro: 2.164 ± 0.348
3.11AsnGln: 3.11 ± 0.43
2.479AsnArg: 2.479 ± 0.347
1.893AsnSer: 1.893 ± 0.409
2.569AsnThr: 2.569 ± 0.347
3.02AsnVal: 3.02 ± 0.374
0.451AsnTrp: 0.451 ± 0.142
1.532AsnTyr: 1.532 ± 0.348
0.0AsnXaa: 0.0 ± 0.0
Pro
4.823ProAla: 4.823 ± 0.645
0.406ProCys: 0.406 ± 0.142
2.704ProAsp: 2.704 ± 0.395
3.966ProGlu: 3.966 ± 0.422
1.487ProPhe: 1.487 ± 0.292
3.11ProGly: 3.11 ± 0.462
0.361ProHis: 0.361 ± 0.146
1.352ProIle: 1.352 ± 0.237
2.118ProLys: 2.118 ± 0.385
3.02ProLeu: 3.02 ± 0.353
1.127ProMet: 1.127 ± 0.241
1.352ProAsn: 1.352 ± 0.341
1.262ProPro: 1.262 ± 0.354
1.532ProGln: 1.532 ± 0.243
1.172ProArg: 1.172 ± 0.23
2.84ProSer: 2.84 ± 0.415
2.704ProThr: 2.704 ± 0.337
3.651ProVal: 3.651 ± 0.502
0.541ProTrp: 0.541 ± 0.172
0.766ProTyr: 0.766 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
6.13GlnAla: 6.13 ± 0.62
0.316GlnCys: 0.316 ± 0.146
2.434GlnAsp: 2.434 ± 0.425
3.155GlnGlu: 3.155 ± 0.354
1.803GlnPhe: 1.803 ± 0.308
3.155GlnGly: 3.155 ± 0.37
0.901GlnHis: 0.901 ± 0.27
2.164GlnIle: 2.164 ± 0.378
2.254GlnLys: 2.254 ± 0.282
4.507GlnLeu: 4.507 ± 0.488
2.118GlnMet: 2.118 ± 0.34
1.397GlnAsn: 1.397 ± 0.24
1.487GlnPro: 1.487 ± 0.338
2.344GlnGln: 2.344 ± 0.352
2.569GlnArg: 2.569 ± 0.38
2.118GlnSer: 2.118 ± 0.314
2.885GlnThr: 2.885 ± 0.597
4.462GlnVal: 4.462 ± 0.517
0.27GlnTrp: 0.27 ± 0.112
1.352GlnTyr: 1.352 ± 0.284
0.0GlnXaa: 0.0 ± 0.0
Arg
4.462ArgAla: 4.462 ± 0.431
0.225ArgCys: 0.225 ± 0.1
3.02ArgAsp: 3.02 ± 0.339
3.245ArgGlu: 3.245 ± 0.368
1.758ArgPhe: 1.758 ± 0.363
3.155ArgGly: 3.155 ± 0.359
1.307ArgHis: 1.307 ± 0.243
3.065ArgIle: 3.065 ± 0.357
2.614ArgLys: 2.614 ± 0.304
5.454ArgLeu: 5.454 ± 0.601
1.442ArgMet: 1.442 ± 0.283
2.704ArgAsn: 2.704 ± 0.468
1.578ArgPro: 1.578 ± 0.274
2.209ArgGln: 2.209 ± 0.31
2.749ArgArg: 2.749 ± 0.44
2.434ArgSer: 2.434 ± 0.24
2.749ArgThr: 2.749 ± 0.369
2.614ArgVal: 2.614 ± 0.344
0.766ArgTrp: 0.766 ± 0.226
1.262ArgTyr: 1.262 ± 0.271
0.0ArgXaa: 0.0 ± 0.0
Ser
6.175SerAla: 6.175 ± 0.747
0.406SerCys: 0.406 ± 0.137
2.795SerAsp: 2.795 ± 0.325
3.2SerGlu: 3.2 ± 0.465
2.073SerPhe: 2.073 ± 0.285
4.597SerGly: 4.597 ± 0.431
0.992SerHis: 0.992 ± 0.225
2.704SerIle: 2.704 ± 0.362
2.84SerLys: 2.84 ± 0.308
5.274SerLeu: 5.274 ± 0.427
1.532SerMet: 1.532 ± 0.257
2.479SerAsn: 2.479 ± 0.265
1.938SerPro: 1.938 ± 0.306
3.02SerGln: 3.02 ± 0.417
2.434SerArg: 2.434 ± 0.335
3.426SerSer: 3.426 ± 0.517
3.335SerThr: 3.335 ± 0.456
4.102SerVal: 4.102 ± 0.489
0.856SerTrp: 0.856 ± 0.209
1.713SerTyr: 1.713 ± 0.273
0.0SerXaa: 0.0 ± 0.0
Thr
6.896ThrAla: 6.896 ± 1.115
0.361ThrCys: 0.361 ± 0.129
3.381ThrAsp: 3.381 ± 0.298
4.282ThrGlu: 4.282 ± 0.485
2.164ThrPhe: 2.164 ± 0.355
4.462ThrGly: 4.462 ± 0.422
0.811ThrHis: 0.811 ± 0.19
3.065ThrIle: 3.065 ± 0.46
3.29ThrLys: 3.29 ± 0.397
4.913ThrLeu: 4.913 ± 0.439
1.397ThrMet: 1.397 ± 0.261
2.885ThrAsn: 2.885 ± 0.359
3.335ThrPro: 3.335 ± 0.399
2.885ThrGln: 2.885 ± 0.378
2.524ThrArg: 2.524 ± 0.315
3.696ThrSer: 3.696 ± 0.47
4.147ThrThr: 4.147 ± 0.455
4.462ThrVal: 4.462 ± 0.458
0.901ThrTrp: 0.901 ± 0.25
2.073ThrTyr: 2.073 ± 0.366
0.0ThrXaa: 0.0 ± 0.0
Val
6.941ValAla: 6.941 ± 0.793
0.947ValCys: 0.947 ± 0.265
4.417ValAsp: 4.417 ± 0.477
4.823ValGlu: 4.823 ± 0.468
2.209ValPhe: 2.209 ± 0.34
4.823ValGly: 4.823 ± 0.58
1.442ValHis: 1.442 ± 0.204
2.524ValIle: 2.524 ± 0.397
3.651ValLys: 3.651 ± 0.442
6.851ValLeu: 6.851 ± 0.665
2.164ValMet: 2.164 ± 0.367
4.102ValAsn: 4.102 ± 0.418
3.426ValPro: 3.426 ± 0.419
2.84ValGln: 2.84 ± 0.371
4.327ValArg: 4.327 ± 0.396
3.831ValSer: 3.831 ± 0.566
5.364ValThr: 5.364 ± 0.461
5.183ValVal: 5.183 ± 0.516
0.992ValTrp: 0.992 ± 0.2
2.389ValTyr: 2.389 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
1.668TrpAla: 1.668 ± 0.281
0.361TrpCys: 0.361 ± 0.149
0.451TrpAsp: 0.451 ± 0.144
0.541TrpGlu: 0.541 ± 0.153
0.406TrpPhe: 0.406 ± 0.144
1.082TrpGly: 1.082 ± 0.206
0.27TrpHis: 0.27 ± 0.119
0.496TrpIle: 0.496 ± 0.17
0.811TrpLys: 0.811 ± 0.214
1.172TrpLeu: 1.172 ± 0.263
0.541TrpMet: 0.541 ± 0.141
0.496TrpAsn: 0.496 ± 0.147
0.631TrpPro: 0.631 ± 0.2
0.496TrpGln: 0.496 ± 0.155
1.037TrpArg: 1.037 ± 0.271
0.496TrpSer: 0.496 ± 0.175
0.631TrpThr: 0.631 ± 0.206
0.947TrpVal: 0.947 ± 0.23
0.0TrpTrp: 0.0 ± 0.0
0.406TrpTyr: 0.406 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.84TyrAla: 2.84 ± 0.351
0.225TyrCys: 0.225 ± 0.118
2.164TyrAsp: 2.164 ± 0.292
2.028TyrGlu: 2.028 ± 0.344
0.901TyrPhe: 0.901 ± 0.267
1.893TyrGly: 1.893 ± 0.254
0.361TyrHis: 0.361 ± 0.137
1.127TyrIle: 1.127 ± 0.253
1.442TyrLys: 1.442 ± 0.269
2.975TyrLeu: 2.975 ± 0.313
0.992TyrMet: 0.992 ± 0.178
1.442TyrAsn: 1.442 ± 0.303
1.217TyrPro: 1.217 ± 0.285
1.127TyrGln: 1.127 ± 0.257
2.028TyrArg: 2.028 ± 0.321
1.262TyrSer: 1.262 ± 0.232
1.442TyrThr: 1.442 ± 0.219
2.434TyrVal: 2.434 ± 0.293
0.586TyrTrp: 0.586 ± 0.178
0.947TyrTyr: 0.947 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (22187 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski