Dragonfly-associated mastrevirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7S1S4|K7S1S4_9GEMI Movement protein OS=Dragonfly-associated mastrevirus OX=1249648 PE=3 SV=1
MM1 pKa = 7.51SSSSAQSGPSHH12 pKa = 6.28FRR14 pKa = 11.84IRR16 pKa = 11.84AQNIFLTYY24 pKa = 9.3PRR26 pKa = 11.84CDD28 pKa = 4.85LDD30 pKa = 4.05PKK32 pKa = 10.82DD33 pKa = 4.04AGEE36 pKa = 4.62IIQSKK41 pKa = 7.32MQSHH45 pKa = 5.89EE46 pKa = 3.69PKK48 pKa = 10.19YY49 pKa = 10.44ILFSRR54 pKa = 11.84EE55 pKa = 3.67LHH57 pKa = 6.66SDD59 pKa = 3.84GEE61 pKa = 4.47YY62 pKa = 10.45HH63 pKa = 7.16LHH65 pKa = 7.17GLLQLSRR72 pKa = 11.84QFSSNNPRR80 pKa = 11.84IFDD83 pKa = 3.5IGAHH87 pKa = 6.05HH88 pKa = 7.22PNIQSAISPKK98 pKa = 9.44SVRR101 pKa = 11.84DD102 pKa = 3.8YY103 pKa = 11.1ILKK106 pKa = 10.5NPITQFCIGTYY117 pKa = 10.2VPAKK121 pKa = 9.94KK122 pKa = 10.02GRR124 pKa = 11.84KK125 pKa = 8.27LGSRR129 pKa = 11.84FEE131 pKa = 4.21EE132 pKa = 4.2NIRR135 pKa = 11.84NNIMRR140 pKa = 11.84SIISTATSKK149 pKa = 10.8EE150 pKa = 4.21PYY152 pKa = 9.9LSMVRR157 pKa = 11.84KK158 pKa = 9.96SFPFEE163 pKa = 3.57WATKK167 pKa = 10.13LSQFEE172 pKa = 4.2YY173 pKa = 10.28SASKK177 pKa = 10.45LFPEE181 pKa = 4.31VTPEE185 pKa = 3.98YY186 pKa = 10.26KK187 pKa = 10.48SPFPTEE193 pKa = 4.2SLICNEE199 pKa = 5.08NIQDD203 pKa = 3.54WVDD206 pKa = 3.08NTLYY210 pKa = 10.33QVSPASYY217 pKa = 10.57CLLHH221 pKa = 6.03PHH223 pKa = 7.23ASATSDD229 pKa = 4.1LLWLQSTSFSLIAPLGGFPSTYY251 pKa = 10.63ADD253 pKa = 3.24QQGQEE258 pKa = 4.07RR259 pKa = 11.84LPGQEE264 pKa = 3.77VV265 pKa = 3.09

Molecular weight: 30.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7RTG7|K7RTG7_9GEMI Replication-associated protein OS=Dragonfly-associated mastrevirus OX=1249648 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.14RR3 pKa = 11.84PSEE6 pKa = 3.86GRR8 pKa = 11.84YY9 pKa = 9.25KK10 pKa = 10.66GNVYY14 pKa = 10.41KK15 pKa = 10.48RR16 pKa = 11.84VKK18 pKa = 10.19PGTSGGNTSGGSVVARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84SGWRR41 pKa = 11.84PSPLQFIQYY50 pKa = 7.68TWTTGGSPITVGAGGYY66 pKa = 8.99VALLTSYY73 pKa = 10.66PRR75 pKa = 11.84GSDD78 pKa = 2.79EE79 pKa = 4.74DD80 pKa = 3.61KK81 pKa = 10.81RR82 pKa = 11.84HH83 pKa = 5.45TGEE86 pKa = 3.8TLTYY90 pKa = 10.44KK91 pKa = 10.7VGLDD95 pKa = 3.46LVFNYY100 pKa = 9.64NSKK103 pKa = 9.39MATGRR108 pKa = 11.84IARR111 pKa = 11.84THH113 pKa = 5.27FCVWLVYY120 pKa = 10.39DD121 pKa = 4.23SQPSGTLPAAHH132 pKa = 7.37GIFDD136 pKa = 6.05LIDD139 pKa = 3.56SFTDD143 pKa = 4.54YY144 pKa = 10.59PATWKK149 pKa = 10.34INRR152 pKa = 11.84DD153 pKa = 3.07MGHH156 pKa = 6.44RR157 pKa = 11.84FVIKK161 pKa = 10.17RR162 pKa = 11.84RR163 pKa = 11.84WVNWLEE169 pKa = 3.95ADD171 pKa = 4.24GSISTSNYY179 pKa = 9.46SGAPSAPAKK188 pKa = 10.53SSVVFNKK195 pKa = 9.4FVKK198 pKa = 10.13RR199 pKa = 11.84LGVRR203 pKa = 11.84TEE205 pKa = 3.93WKK207 pKa = 8.31NTTTGGIGDD216 pKa = 3.75VSKK219 pKa = 10.55GALYY223 pKa = 10.2IVVARR228 pKa = 11.84QLYY231 pKa = 10.21DD232 pKa = 3.02IDD234 pKa = 3.42VRR236 pKa = 11.84GRR238 pKa = 11.84LRR240 pKa = 11.84VYY242 pKa = 10.06FKK244 pKa = 11.14SVGNQQ249 pKa = 2.8

Molecular weight: 27.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

952

94

344

238.0

26.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.357 ± 0.658

1.576 ± 0.415

4.202 ± 0.233

4.412 ± 0.829

4.937 ± 0.477

7.248 ± 1.685

2.206 ± 0.473

6.197 ± 0.919

5.777 ± 0.427

7.143 ± 0.771

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.471 ± 0.126

4.937 ± 0.607

6.092 ± 0.596

3.992 ± 0.785

5.987 ± 0.886

10.504 ± 1.124

6.197 ± 0.967

5.252 ± 1.359

1.891 ± 0.327

4.622 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski