Streptococcus satellite phage Javan738

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A010|A0A4D6A010_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan738 OX=2558832 GN=JavanS738_0014 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.57KK3 pKa = 10.46LLTTSAILLTATVLVACSNNQSATKK28 pKa = 10.36DD29 pKa = 3.27SSEE32 pKa = 4.32QPKK35 pKa = 9.2TEE37 pKa = 4.04QKK39 pKa = 9.3NTTSTNTKK47 pKa = 10.47AKK49 pKa = 10.18VDD51 pKa = 3.49NSKK54 pKa = 11.11YY55 pKa = 10.71DD56 pKa = 3.75DD57 pKa = 5.26LISEE61 pKa = 4.91IKK63 pKa = 10.65LKK65 pKa = 10.68LDD67 pKa = 3.73PEE69 pKa = 4.51STGAISVKK77 pKa = 9.93IQNNVIDD84 pKa = 4.29SDD86 pKa = 3.85SSEE89 pKa = 3.92PHH91 pKa = 6.31DD92 pKa = 5.66AIMILLTGTAKK103 pKa = 10.79DD104 pKa = 3.73SAKK107 pKa = 9.78EE108 pKa = 3.88TMDD111 pKa = 4.95AINSNSATTNQQNAITVFRR130 pKa = 11.84MSISEE135 pKa = 4.15FAKK138 pKa = 10.6KK139 pKa = 10.6LPDD142 pKa = 4.57DD143 pKa = 3.99NTTLSLGYY151 pKa = 9.87EE152 pKa = 4.22KK153 pKa = 10.93SADD156 pKa = 3.72QYY158 pKa = 12.19DD159 pKa = 5.06LIAKK163 pKa = 9.82SSKK166 pKa = 10.33QKK168 pKa = 10.98DD169 pKa = 3.91FIPIGEE175 pKa = 4.84LIVNN179 pKa = 4.54

Molecular weight: 19.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZZJ6|A0A4D5ZZJ6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan738 OX=2558832 GN=JavanS738_0008 PE=4 SV=1
MM1 pKa = 7.12MPKK4 pKa = 10.19SNYY7 pKa = 9.53RR8 pKa = 11.84EE9 pKa = 3.74MRR11 pKa = 11.84FISSLGEE18 pKa = 3.68KK19 pKa = 10.38LLATRR24 pKa = 11.84ILFTVTIANNQQ35 pKa = 3.18

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

3519

35

388

153.0

17.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.916 ± 0.57

0.71 ± 0.128

6.053 ± 0.431

8.07 ± 0.651

4.177 ± 0.371

4.49 ± 0.492

1.591 ± 0.235

7.531 ± 0.438

10.23 ± 0.68

10.372 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.317

6.309 ± 0.619

2.387 ± 0.275

4.007 ± 0.307

4.717 ± 0.442

6.138 ± 0.491

6.422 ± 0.441

4.575 ± 0.444

0.682 ± 0.097

4.433 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski