Gordonia phage Opie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Schnabeltiervirus; unclassified Schnabeltiervirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9L7S8|A0A6B9L7S8_9CAUD Portal protein OS=Gordonia phage Opie OX=2686226 GN=3 PE=4 SV=1
MM1 pKa = 7.88AILFEE6 pKa = 4.66DD7 pKa = 3.68WCTADD12 pKa = 4.86GGTDD16 pKa = 3.34LAGVVTLTPIGQQAAGSLTLATVPKK41 pKa = 9.47PITVADD47 pKa = 4.24GQFSEE52 pKa = 4.84TVTPGDD58 pKa = 3.32YY59 pKa = 9.61MVNVEE64 pKa = 4.43LHH66 pKa = 6.56AGDD69 pKa = 4.34DD70 pKa = 3.98EE71 pKa = 5.07VRR73 pKa = 11.84VSTDD77 pKa = 2.61NAAQRR82 pKa = 11.84IAVADD87 pKa = 3.27IDD89 pKa = 4.04TPQRR93 pKa = 11.84LRR95 pKa = 11.84DD96 pKa = 3.73LVGLGGPGDD105 pKa = 3.97SPLAKK110 pKa = 10.41LNAIVTAWIEE120 pKa = 4.21GVADD124 pKa = 3.71AAYY127 pKa = 10.44APAFPDD133 pKa = 3.19TGITYY138 pKa = 10.24DD139 pKa = 3.47GSGNVQTVTEE149 pKa = 4.78DD150 pKa = 3.66GVATTYY156 pKa = 10.1TYY158 pKa = 11.52NSDD161 pKa = 3.23GTVATDD167 pKa = 3.1TRR169 pKa = 11.84AGVTRR174 pKa = 11.84TYY176 pKa = 10.99GYY178 pKa = 10.7DD179 pKa = 3.29GNGNLTTITVEE190 pKa = 4.21DD191 pKa = 3.65

Molecular weight: 19.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LK52|A0A6B9LK52_9CAUD Major capsid protein OS=Gordonia phage Opie OX=2686226 GN=6 PE=4 SV=1
MM1 pKa = 6.81TQTAAVLAPEE11 pKa = 4.77GTDD14 pKa = 3.33SLLTADD20 pKa = 4.83EE21 pKa = 4.42AASLCGVSAKK31 pKa = 7.59TVRR34 pKa = 11.84AWANRR39 pKa = 11.84GYY41 pKa = 8.69CTRR44 pKa = 11.84GGARR48 pKa = 11.84HH49 pKa = 5.87KK50 pKa = 11.01LEE52 pKa = 3.85VVGRR56 pKa = 11.84DD57 pKa = 3.11EE58 pKa = 5.09RR59 pKa = 11.84GRR61 pKa = 11.84NLYY64 pKa = 9.85RR65 pKa = 11.84ARR67 pKa = 11.84DD68 pKa = 3.68VAKK71 pKa = 10.72AEE73 pKa = 4.23YY74 pKa = 8.31ATSRR78 pKa = 11.84RR79 pKa = 11.84SRR81 pKa = 11.84RR82 pKa = 3.4

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15313

48

1709

209.8

22.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.447 ± 0.497

0.836 ± 0.144

6.557 ± 0.33

5.851 ± 0.345

2.691 ± 0.226

8.496 ± 0.444

1.998 ± 0.172

4.362 ± 0.192

2.965 ± 0.189

8.241 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.011 ± 0.128

2.612 ± 0.195

5.956 ± 0.359

3.526 ± 0.192

7.621 ± 0.362

5.668 ± 0.204

6.57 ± 0.328

7.549 ± 0.27

2.018 ± 0.13

2.024 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski