Methyloligella sp. GL2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Methyloligella; unclassified Methyloligella

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2937 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N0DT04|A0A6N0DT04_9RHIZ PAS domain-containing protein OS=Methyloligella sp. GL2 OX=2742204 GN=HT051_06255 PE=4 SV=1
MM1 pKa = 6.76GQKK4 pKa = 9.98NAGTKK9 pKa = 10.2KK10 pKa = 9.4EE11 pKa = 3.87DD12 pKa = 3.54RR13 pKa = 11.84RR14 pKa = 11.84LMMARR19 pKa = 11.84RR20 pKa = 11.84SYY22 pKa = 11.48AGIIKK27 pKa = 10.37SSFIAAGVLGFAASAHH43 pKa = 5.85ADD45 pKa = 3.49EE46 pKa = 5.37YY47 pKa = 8.51TTGMQQFTGGPGTEE61 pKa = 4.77LEE63 pKa = 4.3LSANVALTTDD73 pKa = 3.6YY74 pKa = 11.07VFRR77 pKa = 11.84GISQTLQDD85 pKa = 4.06PAVSGGFDD93 pKa = 2.94ASYY96 pKa = 10.94GIFYY100 pKa = 10.55LGTWASNVDD109 pKa = 4.16FGGTDD114 pKa = 3.39DD115 pKa = 5.73VEE117 pKa = 4.04IDD119 pKa = 3.84YY120 pKa = 11.19YY121 pKa = 11.57GGVAPTWAGIDD132 pKa = 3.56FDD134 pKa = 5.2FSILAYY140 pKa = 8.69TYY142 pKa = 10.43PGGDD146 pKa = 3.54TDD148 pKa = 4.74TIWEE152 pKa = 4.37GMFAMSKK159 pKa = 10.75SFMDD163 pKa = 4.8DD164 pKa = 3.11KK165 pKa = 11.66LSLGWANYY173 pKa = 9.85FEE175 pKa = 5.43LEE177 pKa = 4.18NGDD180 pKa = 3.43YY181 pKa = 9.54WVPEE185 pKa = 4.11LSAGYY190 pKa = 8.12TFDD193 pKa = 3.52KK194 pKa = 11.13VSIFTPTLSGVIGWVDD210 pKa = 3.66YY211 pKa = 9.07DD212 pKa = 4.04TDD214 pKa = 3.87ASSYY218 pKa = 8.76TYY220 pKa = 10.27WNVGMSLGFYY230 pKa = 9.74KK231 pKa = 10.81DD232 pKa = 4.78DD233 pKa = 3.92MFTLDD238 pKa = 3.86LRR240 pKa = 11.84YY241 pKa = 9.42WDD243 pKa = 4.56SDD245 pKa = 3.45LSAAGCGGDD254 pKa = 4.96IDD256 pKa = 5.31LCDD259 pKa = 3.62EE260 pKa = 4.36RR261 pKa = 11.84VVGTLSASFF270 pKa = 3.64

Molecular weight: 29.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N0E039|A0A6N0E039_9RHIZ DUF1289 domain-containing protein OS=Methyloligella sp. GL2 OX=2742204 GN=HT051_12360 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 8.96IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4AGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2937

0

2937

941881

29

1743

320.7

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.005 ± 0.069

0.862 ± 0.013

5.789 ± 0.033

6.555 ± 0.043

3.802 ± 0.028

8.605 ± 0.053

1.963 ± 0.019

5.168 ± 0.031

3.894 ± 0.039

10.171 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.02

2.556 ± 0.024

5.332 ± 0.033

3.143 ± 0.022

6.359 ± 0.043

5.463 ± 0.034

5.133 ± 0.026

7.028 ± 0.038

1.319 ± 0.019

2.475 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski