Chthonomonas calidirosea (strain DSM 23976 / ICMP 18418 / T49)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Armatimonadetes; Chthonomonadetes; Chthonomonadales; Chthonomonadaceae; Chthonomonas; Chthonomonas calidirosea

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2809 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0EXP8|S0EXP8_CHTCT 30S ribosomal protein S16 OS=Chthonomonas calidirosea (strain DSM 23976 / ICMP 18418 / T49) OX=1303518 GN=rpsP PE=3 SV=1
MM1 pKa = 7.9DD2 pKa = 4.75FSFVTNLNALAASYY16 pKa = 10.58YY17 pKa = 10.88LNQTNSAIQQSLLRR31 pKa = 11.84LSSGLRR37 pKa = 11.84INSPADD43 pKa = 3.65DD44 pKa = 3.96PSGFLIATNLQTQVNGMNQALSNIQNDD71 pKa = 3.49VNEE74 pKa = 4.4VKK76 pKa = 9.47TASGAVQQIVTLVQNIQASALDD98 pKa = 3.95AQSNPSNAAADD109 pKa = 3.66QQAIQSAIAAINNIANNTTFNGIGLLNGTAGTSASVTNSNLIASASFGGTFANGVTQSGNVTITVTQAATMATITGTVNYY189 pKa = 9.8ASANATIANSGTVTINGQSITVSSGEE215 pKa = 4.39TVQSLIDD222 pKa = 4.33SINQISSQTGVSAGFSGGKK241 pKa = 9.2IVLTQTNYY249 pKa = 10.33GGNYY253 pKa = 9.61SISEE257 pKa = 4.16SDD259 pKa = 3.4SAGAIIAAGNATTAVGQNALATVTAMTQINGSTVAQTVNFTGGVNPTDD307 pKa = 3.5SGLLLSDD314 pKa = 3.43SSGNKK319 pKa = 9.29ILLTASGNSTSTSNVAVANISSNPAQFQIGANVGQTVSFAFQNMQASQLGTTAIPGQSLQSINVTTPTGAANAVQIANAALSQVTTYY406 pKa = 11.0AAEE409 pKa = 4.23LGNFQKK415 pKa = 9.43NTLQATQNYY424 pKa = 9.36LSNAVTNLSASVSNIMDD441 pKa = 3.3VNVAQEE447 pKa = 4.44SVNLANLQLLQQSGINALYY466 pKa = 8.55TANNLGALYY475 pKa = 10.61LKK477 pKa = 10.45LLPP480 pKa = 4.82

Molecular weight: 48.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0ETH7|S0ETH7_CHTCT Uncharacterized protein OS=Chthonomonas calidirosea (strain DSM 23976 / ICMP 18418 / T49) OX=1303518 GN=CCALI_00596 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.67QPHH8 pKa = 5.27NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.36KK13 pKa = 9.36RR14 pKa = 11.84VHH16 pKa = 5.97GFRR19 pKa = 11.84SRR21 pKa = 11.84MRR23 pKa = 11.84THH25 pKa = 7.53DD26 pKa = 3.15GRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.13GRR39 pKa = 11.84HH40 pKa = 5.48LLVHH44 pKa = 6.88

Molecular weight: 5.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2809

0

2809

1019034

29

2684

362.8

40.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.841 ± 0.042

1.163 ± 0.017

4.7 ± 0.028

6.063 ± 0.056

3.691 ± 0.028

7.525 ± 0.044

2.5 ± 0.021

5.285 ± 0.033

3.331 ± 0.03

11.044 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.053 ± 0.018

3.214 ± 0.036

5.953 ± 0.04

4.409 ± 0.043

6.614 ± 0.043

5.345 ± 0.037

5.533 ± 0.027

7.138 ± 0.039

1.474 ± 0.023

3.12 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski