Propionibacterium phage E6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Doucettevirus; Propionibacterium virus E6

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8EU62|A0A1D8EU62_9CAUD Tape measure protein OS=Propionibacterium phage E6 OX=1897536 GN=17 PE=4 SV=1
MM1 pKa = 6.9VAWDD5 pKa = 3.8EE6 pKa = 3.97ADD8 pKa = 3.44EE9 pKa = 4.52RR10 pKa = 11.84LDD12 pKa = 3.82TGWMQARR19 pKa = 11.84CPDD22 pKa = 4.13CKK24 pKa = 10.52CFGWIAPDD32 pKa = 3.55EE33 pKa = 4.56EE34 pKa = 5.14GEE36 pKa = 4.3EE37 pKa = 4.48TNGTEE42 pKa = 4.11

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8EU25|A0A1D8EU25_9CAUD Minor tail protein OS=Propionibacterium phage E6 OX=1897536 GN=19 PE=4 SV=1
MM1 pKa = 7.08VALSRR6 pKa = 11.84ASYY9 pKa = 11.56GDD11 pKa = 3.3GTQPTQRR18 pKa = 11.84SDD20 pKa = 3.57GRR22 pKa = 11.84WAASAYY28 pKa = 10.38DD29 pKa = 3.54GWQANGNRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84WVYY43 pKa = 10.21GRR45 pKa = 11.84TQAEE49 pKa = 4.87CKK51 pKa = 10.07RR52 pKa = 11.84KK53 pKa = 9.86LRR55 pKa = 11.84DD56 pKa = 3.38LKK58 pKa = 11.11RR59 pKa = 11.84EE60 pKa = 3.81IWSDD64 pKa = 3.47TQQVNVNPRR73 pKa = 11.84EE74 pKa = 4.29TVKK77 pKa = 10.51SWTASWLDD85 pKa = 3.63DD86 pKa = 3.51YY87 pKa = 11.7RR88 pKa = 11.84SIARR92 pKa = 11.84PTTFATDD99 pKa = 2.96EE100 pKa = 4.36SMVRR104 pKa = 11.84KK105 pKa = 9.51WIVPAVGARR114 pKa = 11.84RR115 pKa = 11.84LSEE118 pKa = 3.79LTARR122 pKa = 11.84DD123 pKa = 3.26ASKK126 pKa = 10.5LQRR129 pKa = 11.84VCRR132 pKa = 11.84DD133 pKa = 3.13GGLSATTSHH142 pKa = 6.62YY143 pKa = 11.24AGLLLRR149 pKa = 11.84RR150 pKa = 11.84ILKK153 pKa = 9.78AARR156 pKa = 11.84ASGYY160 pKa = 10.03RR161 pKa = 11.84IPDD164 pKa = 3.24SVMLARR170 pKa = 11.84IPGIGASNRR179 pKa = 11.84IALSAIQAANLLSTANARR197 pKa = 11.84DD198 pKa = 3.61TWPDD202 pKa = 3.59LPSLPDD208 pKa = 3.57LPYY211 pKa = 11.22GSISKK216 pKa = 9.49LAPGEE221 pKa = 4.02AQKK224 pKa = 10.87RR225 pKa = 11.84EE226 pKa = 3.97QLKK229 pKa = 9.04MEE231 pKa = 4.34RR232 pKa = 11.84LEE234 pKa = 3.8WTAARR239 pKa = 11.84NTDD242 pKa = 3.43PSRR245 pKa = 11.84WAAALMQGLRR255 pKa = 11.84SGEE258 pKa = 3.94ARR260 pKa = 11.84GLTWDD265 pKa = 4.96RR266 pKa = 11.84VDD268 pKa = 4.48LDD270 pKa = 4.23KK271 pKa = 11.32GTITIDD277 pKa = 3.3RR278 pKa = 11.84QLQRR282 pKa = 11.84IKK284 pKa = 10.38PDD286 pKa = 3.11AALPPGYY293 pKa = 10.11KK294 pKa = 8.64VTRR297 pKa = 11.84LEE299 pKa = 4.43GSHH302 pKa = 6.67CLVAPKK308 pKa = 10.1SRR310 pKa = 11.84SGIRR314 pKa = 11.84RR315 pKa = 11.84VPIVPWMGQALTRR328 pKa = 11.84WRR330 pKa = 11.84DD331 pKa = 3.37IQGDD335 pKa = 4.21SPFGLVWPLPTGAPPTRR352 pKa = 11.84VHH354 pKa = 7.58DD355 pKa = 3.95LRR357 pKa = 11.84AWRR360 pKa = 11.84GLQRR364 pKa = 11.84VAGVHH369 pKa = 6.13KK370 pKa = 10.26EE371 pKa = 3.97DD372 pKa = 3.36GSLYY376 pKa = 8.97VLHH379 pKa = 6.94EE380 pKa = 4.58ARR382 pKa = 11.84HH383 pKa = 5.11STVSLLLAAGVPEE396 pKa = 4.29SVVIAIVGHH405 pKa = 6.51ASFAATEE412 pKa = 4.82HH413 pKa = 6.19YY414 pKa = 11.33AHH416 pKa = 7.19TDD418 pKa = 2.9LDD420 pKa = 3.85AARR423 pKa = 11.84AALMKK428 pKa = 10.61VQDD431 pKa = 3.76RR432 pKa = 11.84LGLEE436 pKa = 4.29LEE438 pKa = 4.52SS439 pKa = 4.04

Molecular weight: 48.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11911

38

1364

198.5

21.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.795 ± 0.488

0.898 ± 0.141

6.238 ± 0.377

5.188 ± 0.449

2.628 ± 0.216

8.496 ± 0.537

1.813 ± 0.207

4.66 ± 0.403

3.526 ± 0.279

8.37 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.114

2.049 ± 0.171

5.608 ± 0.337

3.929 ± 0.213

7.111 ± 0.653

6.33 ± 0.334

6.716 ± 0.406

7.287 ± 0.381

2.586 ± 0.183

1.452 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski