Amino acid dipepetide frequency for Microbacterium phage Celaena

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.513AlaAla: 12.513 ± 1.092
0.152AlaCys: 0.152 ± 0.114
6.75AlaAsp: 6.75 ± 0.818
6.067AlaGlu: 6.067 ± 0.546
3.716AlaPhe: 3.716 ± 0.582
11.376AlaGly: 11.376 ± 0.98
2.123AlaHis: 2.123 ± 0.376
6.219AlaIle: 6.219 ± 0.695
5.157AlaLys: 5.157 ± 0.873
10.162AlaLeu: 10.162 ± 1.04
2.123AlaMet: 2.123 ± 0.45
3.489AlaAsn: 3.489 ± 0.474
5.915AlaPro: 5.915 ± 0.627
5.915AlaGln: 5.915 ± 0.622
6.825AlaArg: 6.825 ± 0.826
6.219AlaSer: 6.219 ± 0.835
6.522AlaThr: 6.522 ± 0.756
8.191AlaVal: 8.191 ± 0.845
2.123AlaTrp: 2.123 ± 0.479
2.275AlaTyr: 2.275 ± 0.296
0.0AlaXaa: 0.0 ± 0.0
Cys
0.152CysAla: 0.152 ± 0.105
0.0CysCys: 0.0 ± 0.0
0.228CysAsp: 0.228 ± 0.146
0.152CysGlu: 0.152 ± 0.095
0.152CysPhe: 0.152 ± 0.098
0.834CysGly: 0.834 ± 0.294
0.228CysHis: 0.228 ± 0.164
0.076CysIle: 0.076 ± 0.081
0.152CysLys: 0.152 ± 0.109
0.076CysLeu: 0.076 ± 0.075
0.152CysMet: 0.152 ± 0.11
0.303CysAsn: 0.303 ± 0.162
0.607CysPro: 0.607 ± 0.257
0.076CysGln: 0.076 ± 0.075
0.303CysArg: 0.303 ± 0.161
0.076CysSer: 0.076 ± 0.068
0.152CysThr: 0.152 ± 0.102
0.228CysVal: 0.228 ± 0.122
0.076CysTrp: 0.076 ± 0.087
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.764AspAla: 5.764 ± 0.774
0.152AspCys: 0.152 ± 0.12
3.564AspAsp: 3.564 ± 0.514
3.413AspGlu: 3.413 ± 0.588
2.199AspPhe: 2.199 ± 0.437
7.432AspGly: 7.432 ± 0.919
1.593AspHis: 1.593 ± 0.343
1.517AspIle: 1.517 ± 0.32
1.972AspLys: 1.972 ± 0.404
6.825AspLeu: 6.825 ± 0.802
1.517AspMet: 1.517 ± 0.312
1.213AspAsn: 1.213 ± 0.337
4.55AspPro: 4.55 ± 0.617
1.593AspGln: 1.593 ± 0.28
4.247AspArg: 4.247 ± 0.485
3.109AspSer: 3.109 ± 0.405
4.247AspThr: 4.247 ± 0.553
4.702AspVal: 4.702 ± 0.564
1.365AspTrp: 1.365 ± 0.278
2.427AspTyr: 2.427 ± 0.464
0.0AspXaa: 0.0 ± 0.0
Glu
9.252GluAla: 9.252 ± 0.86
0.152GluCys: 0.152 ± 0.109
4.929GluAsp: 4.929 ± 0.701
4.095GluGlu: 4.095 ± 0.608
3.261GluPhe: 3.261 ± 0.499
3.944GluGly: 3.944 ± 0.48
1.138GluHis: 1.138 ± 0.319
1.062GluIle: 1.062 ± 0.299
1.896GluLys: 1.896 ± 0.341
6.446GluLeu: 6.446 ± 0.672
1.517GluMet: 1.517 ± 0.242
1.82GluAsn: 1.82 ± 0.388
3.185GluPro: 3.185 ± 0.598
3.034GluGln: 3.034 ± 0.4
2.958GluArg: 2.958 ± 0.487
2.351GluSer: 2.351 ± 0.473
3.64GluThr: 3.64 ± 0.477
5.157GluVal: 5.157 ± 0.572
1.668GluTrp: 1.668 ± 0.348
1.441GluTyr: 1.441 ± 0.225
0.0GluXaa: 0.0 ± 0.0
Phe
3.261PheAla: 3.261 ± 0.52
0.228PheCys: 0.228 ± 0.135
2.123PheAsp: 2.123 ± 0.48
3.034PheGlu: 3.034 ± 0.389
0.607PhePhe: 0.607 ± 0.237
2.199PheGly: 2.199 ± 0.449
0.379PheHis: 0.379 ± 0.174
1.517PheIle: 1.517 ± 0.346
1.668PheLys: 1.668 ± 0.349
2.958PheLeu: 2.958 ± 0.537
0.834PheMet: 0.834 ± 0.241
1.289PheAsn: 1.289 ± 0.477
1.441PhePro: 1.441 ± 0.296
1.138PheGln: 1.138 ± 0.267
3.034PheArg: 3.034 ± 0.518
1.365PheSer: 1.365 ± 0.221
2.578PheThr: 2.578 ± 0.49
1.972PheVal: 1.972 ± 0.393
0.531PheTrp: 0.531 ± 0.224
0.683PheTyr: 0.683 ± 0.177
0.0PheXaa: 0.0 ± 0.0
Gly
9.707GlyAla: 9.707 ± 1.096
0.455GlyCys: 0.455 ± 0.169
5.612GlyAsp: 5.612 ± 0.589
5.233GlyGlu: 5.233 ± 0.648
2.654GlyPhe: 2.654 ± 0.478
6.977GlyGly: 6.977 ± 0.884
1.593GlyHis: 1.593 ± 0.415
4.019GlyIle: 4.019 ± 0.712
3.564GlyLys: 3.564 ± 0.64
5.764GlyLeu: 5.764 ± 0.597
2.123GlyMet: 2.123 ± 0.444
2.958GlyAsn: 2.958 ± 0.632
4.247GlyPro: 4.247 ± 0.847
4.019GlyGln: 4.019 ± 0.555
5.233GlyArg: 5.233 ± 0.613
4.778GlySer: 4.778 ± 0.689
5.764GlyThr: 5.764 ± 0.738
6.446GlyVal: 6.446 ± 0.789
2.048GlyTrp: 2.048 ± 0.385
2.958GlyTyr: 2.958 ± 0.402
0.0GlyXaa: 0.0 ± 0.0
His
1.896HisAla: 1.896 ± 0.367
0.076HisCys: 0.076 ± 0.087
1.062HisAsp: 1.062 ± 0.316
0.834HisGlu: 0.834 ± 0.312
0.91HisPhe: 0.91 ± 0.263
1.213HisGly: 1.213 ± 0.293
0.228HisHis: 0.228 ± 0.151
0.531HisIle: 0.531 ± 0.202
0.683HisLys: 0.683 ± 0.22
2.048HisLeu: 2.048 ± 0.482
0.455HisMet: 0.455 ± 0.157
0.379HisAsn: 0.379 ± 0.17
1.213HisPro: 1.213 ± 0.285
0.455HisGln: 0.455 ± 0.172
1.138HisArg: 1.138 ± 0.283
0.758HisSer: 0.758 ± 0.264
0.607HisThr: 0.607 ± 0.194
1.82HisVal: 1.82 ± 0.469
0.531HisTrp: 0.531 ± 0.271
0.986HisTyr: 0.986 ± 0.273
0.0HisXaa: 0.0 ± 0.0
Ile
5.233IleAla: 5.233 ± 0.63
0.152IleCys: 0.152 ± 0.094
2.503IleAsp: 2.503 ± 0.379
3.261IleGlu: 3.261 ± 0.618
0.986IlePhe: 0.986 ± 0.248
3.109IleGly: 3.109 ± 0.514
0.834IleHis: 0.834 ± 0.248
1.82IleIle: 1.82 ± 0.355
2.199IleLys: 2.199 ± 0.501
3.489IleLeu: 3.489 ± 0.677
0.455IleMet: 0.455 ± 0.175
1.062IleAsn: 1.062 ± 0.257
2.123IlePro: 2.123 ± 0.393
1.138IleGln: 1.138 ± 0.293
2.427IleArg: 2.427 ± 0.572
2.048IleSer: 2.048 ± 0.471
4.095IleThr: 4.095 ± 0.557
3.489IleVal: 3.489 ± 0.437
0.683IleTrp: 0.683 ± 0.264
1.213IleTyr: 1.213 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
5.233LysAla: 5.233 ± 0.841
0.455LysCys: 0.455 ± 0.176
2.048LysAsp: 2.048 ± 0.364
1.972LysGlu: 1.972 ± 0.406
0.758LysPhe: 0.758 ± 0.255
3.489LysGly: 3.489 ± 0.442
0.455LysHis: 0.455 ± 0.161
1.213LysIle: 1.213 ± 0.309
2.275LysLys: 2.275 ± 0.489
3.868LysLeu: 3.868 ± 0.733
0.758LysMet: 0.758 ± 0.209
0.91LysAsn: 0.91 ± 0.257
3.109LysPro: 3.109 ± 0.502
1.289LysGln: 1.289 ± 0.329
3.792LysArg: 3.792 ± 0.471
2.427LysSer: 2.427 ± 0.452
3.034LysThr: 3.034 ± 0.364
3.413LysVal: 3.413 ± 0.494
0.455LysTrp: 0.455 ± 0.179
0.91LysTyr: 0.91 ± 0.233
0.0LysXaa: 0.0 ± 0.0
Leu
8.646LeuAla: 8.646 ± 1.126
0.303LeuCys: 0.303 ± 0.155
5.612LeuAsp: 5.612 ± 0.631
5.309LeuGlu: 5.309 ± 0.738
2.199LeuPhe: 2.199 ± 0.378
8.191LeuGly: 8.191 ± 0.841
1.365LeuHis: 1.365 ± 0.38
3.337LeuIle: 3.337 ± 0.529
3.944LeuLys: 3.944 ± 0.519
6.219LeuLeu: 6.219 ± 0.683
2.199LeuMet: 2.199 ± 0.392
2.123LeuAsn: 2.123 ± 0.367
3.944LeuPro: 3.944 ± 0.465
4.626LeuGln: 4.626 ± 0.772
5.991LeuArg: 5.991 ± 0.865
5.233LeuSer: 5.233 ± 0.586
4.778LeuThr: 4.778 ± 0.498
7.356LeuVal: 7.356 ± 0.695
1.062LeuTrp: 1.062 ± 0.31
1.972LeuTyr: 1.972 ± 0.369
0.0LeuXaa: 0.0 ± 0.0
Met
3.337MetAla: 3.337 ± 0.585
0.0MetCys: 0.0 ± 0.0
0.758MetAsp: 0.758 ± 0.277
1.138MetGlu: 1.138 ± 0.282
0.379MetPhe: 0.379 ± 0.175
1.517MetGly: 1.517 ± 0.297
0.379MetHis: 0.379 ± 0.214
0.758MetIle: 0.758 ± 0.208
1.213MetLys: 1.213 ± 0.287
1.289MetLeu: 1.289 ± 0.348
0.076MetMet: 0.076 ± 0.082
1.138MetAsn: 1.138 ± 0.298
0.758MetPro: 0.758 ± 0.225
0.531MetGln: 0.531 ± 0.184
1.365MetArg: 1.365 ± 0.44
1.365MetSer: 1.365 ± 0.327
1.896MetThr: 1.896 ± 0.348
1.365MetVal: 1.365 ± 0.355
0.455MetTrp: 0.455 ± 0.288
0.455MetTyr: 0.455 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
3.109AsnAla: 3.109 ± 0.543
0.228AsnCys: 0.228 ± 0.134
1.289AsnAsp: 1.289 ± 0.323
1.441AsnGlu: 1.441 ± 0.338
0.683AsnPhe: 0.683 ± 0.196
4.095AsnGly: 4.095 ± 0.615
0.834AsnHis: 0.834 ± 0.271
1.441AsnIle: 1.441 ± 0.374
0.834AsnLys: 0.834 ± 0.328
2.503AsnLeu: 2.503 ± 0.441
0.531AsnMet: 0.531 ± 0.216
1.062AsnAsn: 1.062 ± 0.271
2.427AsnPro: 2.427 ± 0.377
1.213AsnGln: 1.213 ± 0.281
1.82AsnArg: 1.82 ± 0.468
1.896AsnSer: 1.896 ± 0.445
1.517AsnThr: 1.517 ± 0.387
1.668AsnVal: 1.668 ± 0.516
0.834AsnTrp: 0.834 ± 0.332
1.138AsnTyr: 1.138 ± 0.266
0.0AsnXaa: 0.0 ± 0.0
Pro
6.674ProAla: 6.674 ± 0.619
0.379ProCys: 0.379 ± 0.195
3.564ProAsp: 3.564 ± 0.672
5.157ProGlu: 5.157 ± 0.829
1.896ProPhe: 1.896 ± 0.333
4.626ProGly: 4.626 ± 0.604
1.213ProHis: 1.213 ± 0.321
3.109ProIle: 3.109 ± 0.5
2.654ProLys: 2.654 ± 0.423
3.716ProLeu: 3.716 ± 0.43
0.683ProMet: 0.683 ± 0.191
1.896ProAsn: 1.896 ± 0.409
1.517ProPro: 1.517 ± 0.328
1.668ProGln: 1.668 ± 0.484
2.048ProArg: 2.048 ± 0.415
2.958ProSer: 2.958 ± 0.47
2.806ProThr: 2.806 ± 0.438
3.868ProVal: 3.868 ± 0.722
1.289ProTrp: 1.289 ± 0.308
0.683ProTyr: 0.683 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
6.143GlnAla: 6.143 ± 0.585
0.152GlnCys: 0.152 ± 0.108
2.351GlnAsp: 2.351 ± 0.389
2.503GlnGlu: 2.503 ± 0.379
1.289GlnPhe: 1.289 ± 0.343
2.958GlnGly: 2.958 ± 0.618
0.531GlnHis: 0.531 ± 0.176
1.062GlnIle: 1.062 ± 0.337
0.607GlnLys: 0.607 ± 0.182
4.095GlnLeu: 4.095 ± 0.591
0.986GlnMet: 0.986 ± 0.308
1.365GlnAsn: 1.365 ± 0.285
1.896GlnPro: 1.896 ± 0.462
1.062GlnGln: 1.062 ± 0.227
1.972GlnArg: 1.972 ± 0.335
2.427GlnSer: 2.427 ± 0.521
2.048GlnThr: 2.048 ± 0.406
3.64GlnVal: 3.64 ± 0.589
0.607GlnTrp: 0.607 ± 0.181
1.062GlnTyr: 1.062 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
6.143ArgAla: 6.143 ± 0.873
0.379ArgCys: 0.379 ± 0.212
4.095ArgAsp: 4.095 ± 0.647
4.474ArgGlu: 4.474 ± 0.743
2.73ArgPhe: 2.73 ± 0.426
4.474ArgGly: 4.474 ± 0.572
1.062ArgHis: 1.062 ± 0.252
2.578ArgIle: 2.578 ± 0.453
2.275ArgLys: 2.275 ± 0.419
6.37ArgLeu: 6.37 ± 0.743
1.213ArgMet: 1.213 ± 0.342
1.593ArgAsn: 1.593 ± 0.262
2.73ArgPro: 2.73 ± 0.418
2.73ArgGln: 2.73 ± 0.372
5.46ArgArg: 5.46 ± 0.755
3.64ArgSer: 3.64 ± 0.471
4.399ArgThr: 4.399 ± 0.636
5.005ArgVal: 5.005 ± 0.675
1.668ArgTrp: 1.668 ± 0.324
1.289ArgTyr: 1.289 ± 0.326
0.0ArgXaa: 0.0 ± 0.0
Ser
6.522SerAla: 6.522 ± 0.746
0.076SerCys: 0.076 ± 0.089
3.868SerAsp: 3.868 ± 0.572
2.351SerGlu: 2.351 ± 0.38
2.351SerPhe: 2.351 ± 0.447
4.702SerGly: 4.702 ± 0.505
0.607SerHis: 0.607 ± 0.228
2.503SerIle: 2.503 ± 0.463
2.882SerLys: 2.882 ± 0.559
4.399SerLeu: 4.399 ± 0.629
0.91SerMet: 0.91 ± 0.221
2.275SerAsn: 2.275 ± 0.41
2.503SerPro: 2.503 ± 0.44
1.365SerGln: 1.365 ± 0.318
3.185SerArg: 3.185 ± 0.383
2.654SerSer: 2.654 ± 0.43
3.716SerThr: 3.716 ± 0.499
5.005SerVal: 5.005 ± 0.73
1.441SerTrp: 1.441 ± 0.361
1.062SerTyr: 1.062 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
8.266ThrAla: 8.266 ± 1.026
0.228ThrCys: 0.228 ± 0.13
4.019ThrAsp: 4.019 ± 0.675
3.716ThrGlu: 3.716 ± 0.499
2.503ThrPhe: 2.503 ± 0.408
5.157ThrGly: 5.157 ± 0.667
0.986ThrHis: 0.986 ± 0.288
4.399ThrIle: 4.399 ± 0.594
3.185ThrLys: 3.185 ± 0.52
4.399ThrLeu: 4.399 ± 0.591
0.683ThrMet: 0.683 ± 0.209
1.441ThrAsn: 1.441 ± 0.377
4.171ThrPro: 4.171 ± 0.54
1.82ThrGln: 1.82 ± 0.445
3.868ThrArg: 3.868 ± 0.722
3.564ThrSer: 3.564 ± 0.588
4.019ThrThr: 4.019 ± 0.867
4.929ThrVal: 4.929 ± 0.705
1.593ThrTrp: 1.593 ± 0.31
1.517ThrTyr: 1.517 ± 0.326
0.0ThrXaa: 0.0 ± 0.0
Val
7.887ValAla: 7.887 ± 0.793
0.076ValCys: 0.076 ± 0.069
5.612ValAsp: 5.612 ± 0.594
5.764ValGlu: 5.764 ± 0.573
2.275ValPhe: 2.275 ± 0.301
5.915ValGly: 5.915 ± 0.991
1.517ValHis: 1.517 ± 0.321
3.489ValIle: 3.489 ± 0.504
3.185ValLys: 3.185 ± 0.502
6.143ValLeu: 6.143 ± 0.669
1.441ValMet: 1.441 ± 0.331
2.199ValAsn: 2.199 ± 0.394
4.019ValPro: 4.019 ± 0.503
3.109ValGln: 3.109 ± 0.431
5.309ValArg: 5.309 ± 0.62
3.944ValSer: 3.944 ± 0.598
5.309ValThr: 5.309 ± 0.717
6.446ValVal: 6.446 ± 0.835
1.972ValTrp: 1.972 ± 0.522
2.578ValTyr: 2.578 ± 0.372
0.0ValXaa: 0.0 ± 0.0
Trp
2.123TrpAla: 2.123 ± 0.431
0.076TrpCys: 0.076 ± 0.077
1.138TrpAsp: 1.138 ± 0.277
1.213TrpGlu: 1.213 ± 0.323
0.683TrpPhe: 0.683 ± 0.244
1.896TrpGly: 1.896 ± 0.357
0.228TrpHis: 0.228 ± 0.141
0.758TrpIle: 0.758 ± 0.224
0.91TrpLys: 0.91 ± 0.272
2.123TrpLeu: 2.123 ± 0.39
0.758TrpMet: 0.758 ± 0.229
1.289TrpAsn: 1.289 ± 0.384
0.607TrpPro: 0.607 ± 0.197
1.213TrpGln: 1.213 ± 0.365
1.062TrpArg: 1.062 ± 0.338
1.593TrpSer: 1.593 ± 0.364
0.986TrpThr: 0.986 ± 0.268
2.048TrpVal: 2.048 ± 0.401
0.303TrpTrp: 0.303 ± 0.124
0.455TrpTyr: 0.455 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.972TyrAla: 1.972 ± 0.335
0.303TyrCys: 0.303 ± 0.168
2.275TyrAsp: 2.275 ± 0.336
1.896TyrGlu: 1.896 ± 0.272
0.607TyrPhe: 0.607 ± 0.251
2.048TyrGly: 2.048 ± 0.39
0.531TyrHis: 0.531 ± 0.163
0.91TyrIle: 0.91 ± 0.214
0.607TyrLys: 0.607 ± 0.219
1.517TyrLeu: 1.517 ± 0.36
0.683TyrMet: 0.683 ± 0.239
0.758TyrAsn: 0.758 ± 0.206
1.441TyrPro: 1.441 ± 0.287
0.758TyrGln: 0.758 ± 0.224
2.275TyrArg: 2.275 ± 0.491
1.972TyrSer: 1.972 ± 0.509
2.351TyrThr: 2.351 ± 0.377
1.441TyrVal: 1.441 ± 0.324
0.758TyrTrp: 0.758 ± 0.241
0.607TyrTyr: 0.607 ± 0.294
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (13187 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski