Amino acid dipepetide frequency for Eubacterium oxidoreducens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.188AlaAla: 6.188 ± 0.12	1.065AlaCys: 1.065 ± 0.038	4.589AlaAsp: 4.589 ± 0.094	4.468AlaGlu: 4.468 ± 0.082	2.819AlaPhe: 2.819 ± 0.077	5.345AlaGly: 5.345 ± 0.1	1.149AlaHis: 1.149 ± 0.04	5.705AlaIle: 5.705 ± 0.084	5.546AlaLys: 5.546 ± 0.104	6.601AlaLeu: 6.601 ± 0.104	2.363AlaMet: 2.363 ± 0.053	3.036AlaAsn: 3.036 ± 0.068	1.8AlaPro: 1.8 ± 0.047	3.065AlaGln: 3.065 ± 0.064	2.68AlaArg: 2.68 ± 0.065	4.451AlaSer: 4.451 ± 0.082	4.077AlaThr: 4.077 ± 0.101	5.385AlaVal: 5.385 ± 0.09	0.513AlaTrp: 0.513 ± 0.022	2.996AlaTyr: 2.996 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	1.054CysAla: 1.054 ± 0.035	0.239CysCys: 0.239 ± 0.02	0.858CysAsp: 0.858 ± 0.037	1.108CysGlu: 1.108 ± 0.039	0.582CysPhe: 0.582 ± 0.026	1.308CysGly: 1.308 ± 0.042	0.277CysHis: 0.277 ± 0.017	1.09CysIle: 1.09 ± 0.034	0.869CysLys: 0.869 ± 0.035	1.041CysLeu: 1.041 ± 0.033	0.417CysMet: 0.417 ± 0.022	0.609CysAsn: 0.609 ± 0.03	0.565CysPro: 0.565 ± 0.027	0.344CysGln: 0.344 ± 0.021	0.539CysArg: 0.539 ± 0.028	0.882CysSer: 0.882 ± 0.037	0.678CysThr: 0.678 ± 0.029	1.073CysVal: 1.073 ± 0.036	0.088CysTrp: 0.088 ± 0.011	0.612CysTyr: 0.612 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	4.676AspAla: 4.676 ± 0.091	0.774AspCys: 0.774 ± 0.03	3.526AspAsp: 3.526 ± 0.084	5.636AspGlu: 5.636 ± 0.094	2.57AspPhe: 2.57 ± 0.061	4.62AspGly: 4.62 ± 0.083	0.929AspHis: 0.929 ± 0.033	4.562AspIle: 4.562 ± 0.079	3.644AspLys: 3.644 ± 0.069	4.769AspLeu: 4.769 ± 0.07	1.799AspMet: 1.799 ± 0.046	2.427AspAsn: 2.427 ± 0.07	1.518AspPro: 1.518 ± 0.048	1.346AspGln: 1.346 ± 0.042	2.118AspArg: 2.118 ± 0.059	3.467AspSer: 3.467 ± 0.078	3.3AspThr: 3.3 ± 0.089	4.224AspVal: 4.224 ± 0.077	0.484AspTrp: 0.484 ± 0.027	3.269AspTyr: 3.269 ± 0.072	0.0AspXaa: 0.0 ± 0.0
Glu	5.506GluAla: 5.506 ± 0.103	0.844GluCys: 0.844 ± 0.034	5.06GluAsp: 5.06 ± 0.092	7.839GluGlu: 7.839 ± 0.141	2.689GluPhe: 2.689 ± 0.062	4.338GluGly: 4.338 ± 0.086	1.566GluHis: 1.566 ± 0.048	6.068GluIle: 6.068 ± 0.096	6.16GluLys: 6.16 ± 0.115	6.752GluLeu: 6.752 ± 0.101	2.484GluMet: 2.484 ± 0.059	4.009GluAsn: 4.009 ± 0.073	1.716GluPro: 1.716 ± 0.058	3.349GluGln: 3.349 ± 0.08	3.181GluArg: 3.181 ± 0.077	3.947GluSer: 3.947 ± 0.087	3.824GluThr: 3.824 ± 0.088	4.925GluVal: 4.925 ± 0.079	0.547GluTrp: 0.547 ± 0.027	3.501GluTyr: 3.501 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	2.985PheAla: 2.985 ± 0.062	0.614PheCys: 0.614 ± 0.026	2.692PheAsp: 2.692 ± 0.055	3.027PheGlu: 3.027 ± 0.062	1.648PhePhe: 1.648 ± 0.057	2.659PheGly: 2.659 ± 0.061	0.757PheHis: 0.757 ± 0.033	2.436PheIle: 2.436 ± 0.057	2.079PheLys: 2.079 ± 0.049	3.303PheLeu: 3.303 ± 0.084	1.094PheMet: 1.094 ± 0.035	1.366PheAsn: 1.366 ± 0.036	1.124PhePro: 1.124 ± 0.04	1.05PheGln: 1.05 ± 0.033	1.424PheArg: 1.424 ± 0.044	2.721PheSer: 2.721 ± 0.066	2.19PheThr: 2.19 ± 0.056	2.938PheVal: 2.938 ± 0.073	0.388PheTrp: 0.388 ± 0.025	1.668PheTyr: 1.668 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	4.826GlyAla: 4.826 ± 0.093	1.26GlyCys: 1.26 ± 0.046	3.559GlyAsp: 3.559 ± 0.073	4.495GlyGlu: 4.495 ± 0.082	2.776GlyPhe: 2.776 ± 0.053	4.519GlyGly: 4.519 ± 0.106	1.24GlyHis: 1.24 ± 0.041	5.798GlyIle: 5.798 ± 0.1	5.216GlyLys: 5.216 ± 0.089	5.211GlyLeu: 5.211 ± 0.086	2.279GlyMet: 2.279 ± 0.056	3.123GlyAsn: 3.123 ± 0.073	1.175GlyPro: 1.175 ± 0.038	1.899GlyGln: 1.899 ± 0.047	2.592GlyArg: 2.592 ± 0.06	4.095GlySer: 4.095 ± 0.077	4.324GlyThr: 4.324 ± 0.118	4.743GlyVal: 4.743 ± 0.085	0.617GlyTrp: 0.617 ± 0.031	3.422GlyTyr: 3.422 ± 0.073	0.0GlyXaa: 0.0 ± 0.0
His	1.138HisAla: 1.138 ± 0.04	0.275HisCys: 0.275 ± 0.018	0.953HisAsp: 0.953 ± 0.034	1.173HisGlu: 1.173 ± 0.042	0.769HisPhe: 0.769 ± 0.027	1.185HisGly: 1.185 ± 0.035	0.419HisHis: 0.419 ± 0.031	1.337HisIle: 1.337 ± 0.041	1.116HisLys: 1.116 ± 0.035	1.554HisLeu: 1.554 ± 0.047	0.545HisMet: 0.545 ± 0.027	0.832HisAsn: 0.832 ± 0.033	0.846HisPro: 0.846 ± 0.032	0.503HisGln: 0.503 ± 0.023	0.677HisArg: 0.677 ± 0.028	0.991HisSer: 0.991 ± 0.035	0.906HisThr: 0.906 ± 0.032	1.072HisVal: 1.072 ± 0.037	0.129HisTrp: 0.129 ± 0.013	0.781HisTyr: 0.781 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.111IleAla: 6.111 ± 0.098	1.315IleCys: 1.315 ± 0.04	4.437IleAsp: 4.437 ± 0.073	5.603IleGlu: 5.603 ± 0.093	2.759IlePhe: 2.759 ± 0.066	5.155IleGly: 5.155 ± 0.091	1.348IleHis: 1.348 ± 0.041	5.437IleIle: 5.437 ± 0.104	4.992IleLys: 4.992 ± 0.089	6.389IleLeu: 6.389 ± 0.116	2.003IleMet: 2.003 ± 0.056	3.063IleAsn: 3.063 ± 0.051	2.697IlePro: 2.697 ± 0.052	2.075IleGln: 2.075 ± 0.049	3.35IleArg: 3.35 ± 0.07	5.235IleSer: 5.235 ± 0.089	4.711IleThr: 4.711 ± 0.121	5.502IleVal: 5.502 ± 0.097	0.574IleTrp: 0.574 ± 0.029	3.001IleTyr: 3.001 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	5.386LysAla: 5.386 ± 0.097	0.79LysCys: 0.79 ± 0.029	4.065LysAsp: 4.065 ± 0.07	7.451LysGlu: 7.451 ± 0.137	1.804LysPhe: 1.804 ± 0.049	4.124LysGly: 4.124 ± 0.073	1.081LysHis: 1.081 ± 0.033	5.333LysIle: 5.333 ± 0.089	7.169LysLys: 7.169 ± 0.132	5.312LysLeu: 5.312 ± 0.09	2.449LysMet: 2.449 ± 0.059	3.739LysAsn: 3.739 ± 0.075	1.837LysPro: 1.837 ± 0.052	2.593LysGln: 2.593 ± 0.064	2.949LysArg: 2.949 ± 0.069	3.89LysSer: 3.89 ± 0.072	4.049LysThr: 4.049 ± 0.078	5.029LysVal: 5.029 ± 0.091	0.561LysTrp: 0.561 ± 0.027	2.957LysTyr: 2.957 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	6.341LeuAla: 6.341 ± 0.082	1.331LeuCys: 1.331 ± 0.047	5.06LeuAsp: 5.06 ± 0.091	6.568LeuGlu: 6.568 ± 0.095	3.37LeuPhe: 3.37 ± 0.081	5.385LeuGly: 5.385 ± 0.09	1.458LeuHis: 1.458 ± 0.052	6.002LeuIle: 6.002 ± 0.107	6.211LeuLys: 6.211 ± 0.101	7.479LeuLeu: 7.479 ± 0.144	2.599LeuMet: 2.599 ± 0.066	3.758LeuAsn: 3.758 ± 0.067	2.868LeuPro: 2.868 ± 0.061	2.61LeuGln: 2.61 ± 0.063	3.341LeuArg: 3.341 ± 0.063	6.108LeuSer: 6.108 ± 0.095	4.991LeuThr: 4.991 ± 0.1	5.507LeuVal: 5.507 ± 0.075	0.565LeuTrp: 0.565 ± 0.027	3.303LeuTyr: 3.303 ± 0.069	0.001LeuXaa: 0.001 ± 0.001
Met	2.285MetAla: 2.285 ± 0.055	0.378MetCys: 0.378 ± 0.019	1.901MetAsp: 1.901 ± 0.049	2.404MetGlu: 2.404 ± 0.056	0.977MetPhe: 0.977 ± 0.041	1.882MetGly: 1.882 ± 0.05	0.534MetHis: 0.534 ± 0.028	2.246MetIle: 2.246 ± 0.062	2.561MetLys: 2.561 ± 0.057	2.642MetLeu: 2.642 ± 0.064	1.008MetMet: 1.008 ± 0.034	1.56MetAsn: 1.56 ± 0.047	1.149MetPro: 1.149 ± 0.037	1.175MetGln: 1.175 ± 0.042	1.276MetArg: 1.276 ± 0.041	1.894MetSer: 1.894 ± 0.045	1.851MetThr: 1.851 ± 0.045	1.887MetVal: 1.887 ± 0.056	0.173MetTrp: 0.173 ± 0.014	0.921MetTyr: 0.921 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.352AsnAla: 3.352 ± 0.074	0.639AsnCys: 0.639 ± 0.028	2.31AsnAsp: 2.31 ± 0.055	3.36AsnGlu: 3.36 ± 0.071	1.467AsnPhe: 1.467 ± 0.042	3.341AsnGly: 3.341 ± 0.078	0.956AsnHis: 0.956 ± 0.035	3.507AsnIle: 3.507 ± 0.072	2.905AsnLys: 2.905 ± 0.061	4.014AsnLeu: 4.014 ± 0.067	1.313AsnMet: 1.313 ± 0.038	2.103AsnAsn: 2.103 ± 0.061	1.92AsnPro: 1.92 ± 0.05	1.521AsnGln: 1.521 ± 0.049	1.976AsnArg: 1.976 ± 0.054	2.425AsnSer: 2.425 ± 0.052	2.535AsnThr: 2.535 ± 0.075	3.121AsnVal: 3.121 ± 0.06	0.367AsnTrp: 0.367 ± 0.022	2.104AsnTyr: 2.104 ± 0.063	0.0AsnXaa: 0.0 ± 0.0
Pro	1.962ProAla: 1.962 ± 0.055	0.443ProCys: 0.443 ± 0.024	2.026ProAsp: 2.026 ± 0.049	2.374ProGlu: 2.374 ± 0.065	1.294ProPhe: 1.294 ± 0.041	1.883ProGly: 1.883 ± 0.051	0.497ProHis: 0.497 ± 0.025	2.173ProIle: 2.173 ± 0.052	2.031ProLys: 2.031 ± 0.052	2.344ProLeu: 2.344 ± 0.056	0.811ProMet: 0.811 ± 0.033	1.288ProAsn: 1.288 ± 0.037	0.631ProPro: 0.631 ± 0.035	1.108ProGln: 1.108 ± 0.038	0.905ProArg: 0.905 ± 0.034	1.696ProSer: 1.696 ± 0.05	1.647ProThr: 1.647 ± 0.049	2.287ProVal: 2.287 ± 0.053	0.246ProTrp: 0.246 ± 0.018	1.35ProTyr: 1.35 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.327GlnAla: 2.327 ± 0.056	0.353GlnCys: 0.353 ± 0.019	1.747GlnAsp: 1.747 ± 0.048	2.89GlnGlu: 2.89 ± 0.056	1.168GlnPhe: 1.168 ± 0.041	1.909GlnGly: 1.909 ± 0.045	0.463GlnHis: 0.463 ± 0.024	2.854GlnIle: 2.854 ± 0.059	2.617GlnLys: 2.617 ± 0.056	3.124GlnLeu: 3.124 ± 0.069	1.297GlnMet: 1.297 ± 0.047	1.601GlnAsn: 1.601 ± 0.041	0.853GlnPro: 0.853 ± 0.032	1.207GlnGln: 1.207 ± 0.04	1.346GlnArg: 1.346 ± 0.047	1.846GlnSer: 1.846 ± 0.051	1.835GlnThr: 1.835 ± 0.052	2.232GlnVal: 2.232 ± 0.052	0.222GlnTrp: 0.222 ± 0.018	1.386GlnTyr: 1.386 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	2.519ArgAla: 2.519 ± 0.058	0.559ArgCys: 0.559 ± 0.027	1.908ArgAsp: 1.908 ± 0.053	3.129ArgGlu: 3.129 ± 0.071	1.755ArgPhe: 1.755 ± 0.047	2.254ArgGly: 2.254 ± 0.066	0.689ArgHis: 0.689 ± 0.029	3.277ArgIle: 3.277 ± 0.072	3.072ArgLys: 3.072 ± 0.073	3.443ArgLeu: 3.443 ± 0.077	1.483ArgMet: 1.483 ± 0.046	1.883ArgAsn: 1.883 ± 0.057	1.095ArgPro: 1.095 ± 0.041	1.53ArgGln: 1.53 ± 0.046	2.012ArgArg: 2.012 ± 0.063	1.972ArgSer: 1.972 ± 0.053	1.906ArgThr: 1.906 ± 0.048	2.501ArgVal: 2.501 ± 0.054	0.328ArgTrp: 0.328 ± 0.02	1.757ArgTyr: 1.757 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	4.254SerAla: 4.254 ± 0.075	0.791SerCys: 0.791 ± 0.036	4.013SerAsp: 4.013 ± 0.093	4.138SerGlu: 4.138 ± 0.075	2.563SerPhe: 2.563 ± 0.059	4.912SerGly: 4.912 ± 0.094	1.026SerHis: 1.026 ± 0.036	4.326SerIle: 4.326 ± 0.067	4.279SerLys: 4.279 ± 0.081	5.144SerLeu: 5.144 ± 0.087	1.831SerMet: 1.831 ± 0.05	2.783SerAsn: 2.783 ± 0.075	1.554SerPro: 1.554 ± 0.046	2.055SerGln: 2.055 ± 0.052	2.141SerArg: 2.141 ± 0.054	4.601SerSer: 4.601 ± 0.155	3.359SerThr: 3.359 ± 0.098	4.478SerVal: 4.478 ± 0.083	0.476SerTrp: 0.476 ± 0.023	2.96SerTyr: 2.96 ± 0.094	0.0SerXaa: 0.0 ± 0.0
Thr	4.263ThrAla: 4.263 ± 0.113	0.683ThrCys: 0.683 ± 0.029	3.447ThrAsp: 3.447 ± 0.084	3.517ThrGlu: 3.517 ± 0.076	2.254ThrPhe: 2.254 ± 0.066	4.422ThrGly: 4.422 ± 0.087	0.828ThrHis: 0.828 ± 0.031	4.873ThrIle: 4.873 ± 0.125	3.909ThrLys: 3.909 ± 0.073	4.923ThrLeu: 4.923 ± 0.113	1.531ThrMet: 1.531 ± 0.043	2.509ThrAsn: 2.509 ± 0.077	1.955ThrPro: 1.955 ± 0.043	2.017ThrGln: 2.017 ± 0.061	1.737ThrArg: 1.737 ± 0.049	3.6ThrSer: 3.6 ± 0.118	3.672ThrThr: 3.672 ± 0.127	4.313ThrVal: 4.313 ± 0.111	0.427ThrTrp: 0.427 ± 0.025	2.713ThrTyr: 2.713 ± 0.12	0.0ThrXaa: 0.0 ± 0.0
Val	5.147ValAla: 5.147 ± 0.076	1.164ValCys: 1.164 ± 0.044	4.135ValAsp: 4.135 ± 0.072	4.847ValGlu: 4.847 ± 0.084	2.756ValPhe: 2.756 ± 0.057	4.411ValGly: 4.411 ± 0.074	1.008ValHis: 1.008 ± 0.036	5.391ValIle: 5.391 ± 0.085	4.831ValLys: 4.831 ± 0.086	6.162ValLeu: 6.162 ± 0.095	2.063ValMet: 2.063 ± 0.049	3.108ValAsn: 3.108 ± 0.077	2.16ValPro: 2.16 ± 0.055	2.032ValGln: 2.032 ± 0.048	2.684ValArg: 2.684 ± 0.063	4.731ValSer: 4.731 ± 0.098	4.609ValThr: 4.609 ± 0.127	5.255ValVal: 5.255 ± 0.094	0.542ValTrp: 0.542 ± 0.027	2.851ValTyr: 2.851 ± 0.065	0.0ValXaa: 0.0 ± 0.0
Trp	0.473TrpAla: 0.473 ± 0.025	0.093TrpCys: 0.093 ± 0.01	0.498TrpAsp: 0.498 ± 0.026	0.635TrpGlu: 0.635 ± 0.025	0.315TrpPhe: 0.315 ± 0.021	0.539TrpGly: 0.539 ± 0.029	0.15TrpHis: 0.15 ± 0.014	0.57TrpIle: 0.57 ± 0.026	0.59TrpLys: 0.59 ± 0.027	0.707TrpLeu: 0.707 ± 0.029	0.259TrpMet: 0.259 ± 0.018	0.439TrpAsn: 0.439 ± 0.022	0.158TrpPro: 0.158 ± 0.014	0.277TrpGln: 0.277 ± 0.016	0.259TrpArg: 0.259 ± 0.016	0.425TrpSer: 0.425 ± 0.024	0.412TrpThr: 0.412 ± 0.022	0.418TrpVal: 0.418 ± 0.027	0.065TrpTrp: 0.065 ± 0.008	0.354TrpTyr: 0.354 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.049TyrAla: 3.049 ± 0.07	0.623TyrCys: 0.623 ± 0.028	2.875TyrAsp: 2.875 ± 0.07	3.641TyrGlu: 3.641 ± 0.065	1.828TyrPhe: 1.828 ± 0.053	2.976TyrGly: 2.976 ± 0.067	0.825TyrHis: 0.825 ± 0.031	2.774TyrIle: 2.774 ± 0.06	2.774TyrLys: 2.774 ± 0.065	3.941TyrLeu: 3.941 ± 0.078	1.074TyrMet: 1.074 ± 0.035	1.997TyrAsn: 1.997 ± 0.05	1.377TyrPro: 1.377 ± 0.041	1.522TyrGln: 1.522 ± 0.042	1.886TyrArg: 1.886 ± 0.052	2.709TyrSer: 2.709 ± 0.07	2.72TyrThr: 2.72 ± 0.115	2.993TyrVal: 2.993 ± 0.062	0.322TyrTrp: 0.322 ± 0.019	2.253TyrTyr: 2.253 ± 0.088	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.003

Statistics based on 2618 proteins (873114 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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