Pseudomonas phage vB_PaeP_PAO1_Ab05

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; Phikmvvirus; Pseudomonas virus Ab05

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1IUQ1|A0A0A1IUQ1_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_PAO1_Ab05 OX=1548902 GN=ORF05 PE=4 SV=1
MM1 pKa = 7.32LCEE4 pKa = 4.98HH5 pKa = 7.13PLIDD9 pKa = 3.9PTTQAGLIRR18 pKa = 11.84AVAAYY23 pKa = 10.08QDD25 pKa = 5.01ALDD28 pKa = 4.47LCNALNQGDD37 pKa = 3.63

Molecular weight: 3.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1IWK1|A0A0A1IWK1_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_PAO1_Ab05 OX=1548902 GN=ORF11 PE=4 SV=1
MM1 pKa = 7.71SSRR4 pKa = 11.84DD5 pKa = 3.56PYY7 pKa = 10.8RR8 pKa = 11.84IGHH11 pKa = 6.13RR12 pKa = 11.84VGLVNYY18 pKa = 9.6SDD20 pKa = 4.68RR21 pKa = 11.84YY22 pKa = 10.32LGADD26 pKa = 3.1AAGTKK31 pKa = 10.38GIIEE35 pKa = 5.36AITRR39 pKa = 11.84PSRR42 pKa = 11.84CMTIYY47 pKa = 10.37HH48 pKa = 5.35VRR50 pKa = 11.84CEE52 pKa = 3.79RR53 pKa = 11.84TLRR56 pKa = 11.84LIEE59 pKa = 4.13AEE61 pKa = 3.77ARR63 pKa = 11.84NVRR66 pKa = 11.84FIRR69 pKa = 11.84QLPTRR74 pKa = 11.84KK75 pKa = 9.78

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13357

37

1337

247.4

27.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.148 ± 0.503

1.003 ± 0.183

5.952 ± 0.213

6.012 ± 0.23

3.212 ± 0.177

7.913 ± 0.301

2.119 ± 0.178

4.237 ± 0.185

4.664 ± 0.25

8.969 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.987 ± 0.111

3.639 ± 0.223

4.552 ± 0.324

4.634 ± 0.271

7.052 ± 0.343

4.672 ± 0.198

5.503 ± 0.326

7.097 ± 0.248

1.587 ± 0.133

3.047 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski