Amino acid dipepetide frequency for Pectobacterium phage ZF40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.648AlaAla: 11.648 ± 1.408
0.79AlaCys: 0.79 ± 0.258
7.041AlaAsp: 7.041 ± 0.734
5.33AlaGlu: 5.33 ± 0.593
3.159AlaPhe: 3.159 ± 0.48
9.739AlaGly: 9.739 ± 1.139
1.25AlaHis: 1.25 ± 0.266
6.054AlaIle: 6.054 ± 0.638
4.212AlaLys: 4.212 ± 0.591
8.028AlaLeu: 8.028 ± 0.84
3.29AlaMet: 3.29 ± 0.411
4.738AlaAsn: 4.738 ± 0.59
3.685AlaPro: 3.685 ± 0.586
4.08AlaGln: 4.08 ± 0.486
5.462AlaArg: 5.462 ± 0.564
5.594AlaSer: 5.594 ± 0.717
5.33AlaThr: 5.33 ± 0.76
5.857AlaVal: 5.857 ± 0.555
1.25AlaTrp: 1.25 ± 0.347
3.027AlaTyr: 3.027 ± 0.433
0.0AlaXaa: 0.0 ± 0.0
Cys
0.724CysAla: 0.724 ± 0.216
0.197CysCys: 0.197 ± 0.113
0.79CysAsp: 0.79 ± 0.22
0.724CysGlu: 0.724 ± 0.188
0.395CysPhe: 0.395 ± 0.143
0.987CysGly: 0.987 ± 0.229
0.329CysHis: 0.329 ± 0.186
0.329CysIle: 0.329 ± 0.16
0.526CysLys: 0.526 ± 0.206
0.592CysLeu: 0.592 ± 0.177
0.197CysMet: 0.197 ± 0.103
0.395CysAsn: 0.395 ± 0.159
0.526CysPro: 0.526 ± 0.215
0.526CysGln: 0.526 ± 0.197
0.724CysArg: 0.724 ± 0.208
0.395CysSer: 0.395 ± 0.139
0.263CysThr: 0.263 ± 0.139
0.526CysVal: 0.526 ± 0.195
0.461CysTrp: 0.461 ± 0.148
0.197CysTyr: 0.197 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
6.844AspAla: 6.844 ± 0.602
0.461AspCys: 0.461 ± 0.164
3.751AspAsp: 3.751 ± 0.576
4.343AspGlu: 4.343 ± 0.45
2.566AspPhe: 2.566 ± 0.562
6.383AspGly: 6.383 ± 0.677
1.25AspHis: 1.25 ± 0.244
5.067AspIle: 5.067 ± 0.536
2.566AspLys: 2.566 ± 0.375
3.422AspLeu: 3.422 ± 0.496
2.106AspMet: 2.106 ± 0.3
2.632AspAsn: 2.632 ± 0.492
2.303AspPro: 2.303 ± 0.33
2.501AspGln: 2.501 ± 0.302
2.764AspArg: 2.764 ± 0.297
4.212AspSer: 4.212 ± 0.566
2.435AspThr: 2.435 ± 0.404
4.08AspVal: 4.08 ± 0.529
1.053AspTrp: 1.053 ± 0.236
2.106AspTyr: 2.106 ± 0.332
0.0AspXaa: 0.0 ± 0.0
Glu
3.751GluAla: 3.751 ± 0.481
0.79GluCys: 0.79 ± 0.227
2.172GluAsp: 2.172 ± 0.382
2.895GluGlu: 2.895 ± 0.653
2.895GluPhe: 2.895 ± 0.376
2.501GluGly: 2.501 ± 0.334
0.921GluHis: 0.921 ± 0.263
5.33GluIle: 5.33 ± 0.506
3.554GluLys: 3.554 ± 0.559
4.409GluLeu: 4.409 ± 0.688
1.777GluMet: 1.777 ± 0.344
3.685GluAsn: 3.685 ± 0.53
1.711GluPro: 1.711 ± 0.371
2.501GluGln: 2.501 ± 0.393
4.277GluArg: 4.277 ± 0.595
3.817GluSer: 3.817 ± 0.548
3.356GluThr: 3.356 ± 0.514
2.435GluVal: 2.435 ± 0.381
0.921GluTrp: 0.921 ± 0.251
2.04GluTyr: 2.04 ± 0.332
0.0GluXaa: 0.0 ± 0.0
Phe
2.764PheAla: 2.764 ± 0.384
0.329PheCys: 0.329 ± 0.148
2.369PheAsp: 2.369 ± 0.327
1.448PheGlu: 1.448 ± 0.279
1.316PhePhe: 1.316 ± 0.291
3.225PheGly: 3.225 ± 0.411
0.987PheHis: 0.987 ± 0.239
1.514PheIle: 1.514 ± 0.277
1.777PheLys: 1.777 ± 0.37
2.566PheLeu: 2.566 ± 0.471
0.921PheMet: 0.921 ± 0.256
1.645PheAsn: 1.645 ± 0.297
1.448PhePro: 1.448 ± 0.259
1.316PheGln: 1.316 ± 0.233
2.172PheArg: 2.172 ± 0.368
3.685PheSer: 3.685 ± 0.478
1.974PheThr: 1.974 ± 0.323
1.843PheVal: 1.843 ± 0.401
0.658PheTrp: 0.658 ± 0.2
0.987PheTyr: 0.987 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
7.173GlyAla: 7.173 ± 0.962
0.526GlyCys: 0.526 ± 0.188
3.948GlyAsp: 3.948 ± 0.403
4.475GlyGlu: 4.475 ± 0.629
3.027GlyPhe: 3.027 ± 0.431
7.305GlyGly: 7.305 ± 1.149
1.711GlyHis: 1.711 ± 0.343
3.619GlyIle: 3.619 ± 0.516
3.356GlyLys: 3.356 ± 0.361
5.33GlyLeu: 5.33 ± 0.649
2.83GlyMet: 2.83 ± 0.394
3.093GlyAsn: 3.093 ± 0.491
1.316GlyPro: 1.316 ± 0.3
3.422GlyGln: 3.422 ± 0.508
3.948GlyArg: 3.948 ± 0.456
4.014GlySer: 4.014 ± 0.592
5.396GlyThr: 5.396 ± 0.5
6.646GlyVal: 6.646 ± 0.567
1.25GlyTrp: 1.25 ± 0.201
2.435GlyTyr: 2.435 ± 0.4
0.0GlyXaa: 0.0 ± 0.0
His
1.25HisAla: 1.25 ± 0.282
0.066HisCys: 0.066 ± 0.059
0.987HisAsp: 0.987 ± 0.215
0.855HisGlu: 0.855 ± 0.232
0.79HisPhe: 0.79 ± 0.216
0.921HisGly: 0.921 ± 0.238
0.395HisHis: 0.395 ± 0.157
1.382HisIle: 1.382 ± 0.273
0.526HisLys: 0.526 ± 0.179
1.119HisLeu: 1.119 ± 0.244
0.197HisMet: 0.197 ± 0.1
0.592HisAsn: 0.592 ± 0.174
0.921HisPro: 0.921 ± 0.212
0.526HisGln: 0.526 ± 0.179
0.724HisArg: 0.724 ± 0.171
1.119HisSer: 1.119 ± 0.34
1.185HisThr: 1.185 ± 0.28
1.316HisVal: 1.316 ± 0.248
0.263HisTrp: 0.263 ± 0.127
0.921HisTyr: 0.921 ± 0.183
0.0HisXaa: 0.0 ± 0.0
Ile
6.976IleAla: 6.976 ± 0.521
0.658IleCys: 0.658 ± 0.207
4.804IleAsp: 4.804 ± 0.525
4.277IleGlu: 4.277 ± 0.641
1.645IlePhe: 1.645 ± 0.354
3.619IleGly: 3.619 ± 0.527
0.79IleHis: 0.79 ± 0.203
3.29IleIle: 3.29 ± 0.471
3.948IleLys: 3.948 ± 0.561
3.356IleLeu: 3.356 ± 0.53
0.987IleMet: 0.987 ± 0.244
3.488IleAsn: 3.488 ± 0.487
3.619IlePro: 3.619 ± 0.494
2.237IleGln: 2.237 ± 0.453
2.895IleArg: 2.895 ± 0.413
4.672IleSer: 4.672 ± 0.555
5.001IleThr: 5.001 ± 0.452
3.225IleVal: 3.225 ± 0.484
0.79IleTrp: 0.79 ± 0.207
1.448IleTyr: 1.448 ± 0.323
0.0IleXaa: 0.0 ± 0.0
Lys
4.212LysAla: 4.212 ± 0.528
0.461LysCys: 0.461 ± 0.197
1.908LysAsp: 1.908 ± 0.347
2.566LysGlu: 2.566 ± 0.521
1.711LysPhe: 1.711 ± 0.334
2.303LysGly: 2.303 ± 0.418
0.526LysHis: 0.526 ± 0.173
3.422LysIle: 3.422 ± 0.436
3.093LysLys: 3.093 ± 0.476
4.343LysLeu: 4.343 ± 0.534
1.382LysMet: 1.382 ± 0.323
2.106LysAsn: 2.106 ± 0.477
2.501LysPro: 2.501 ± 0.369
1.711LysGln: 1.711 ± 0.316
3.619LysArg: 3.619 ± 0.536
3.619LysSer: 3.619 ± 0.464
4.08LysThr: 4.08 ± 0.575
1.448LysVal: 1.448 ± 0.265
0.987LysTrp: 0.987 ± 0.223
1.448LysTyr: 1.448 ± 0.403
0.0LysXaa: 0.0 ± 0.0
Leu
7.37LeuAla: 7.37 ± 0.55
0.855LeuCys: 0.855 ± 0.262
5.462LeuAsp: 5.462 ± 0.621
3.817LeuGlu: 3.817 ± 0.583
2.04LeuPhe: 2.04 ± 0.374
4.409LeuGly: 4.409 ± 0.524
0.987LeuHis: 0.987 ± 0.243
5.133LeuIle: 5.133 ± 0.577
3.751LeuLys: 3.751 ± 0.503
5.659LeuLeu: 5.659 ± 0.653
1.777LeuMet: 1.777 ± 0.363
4.672LeuAsn: 4.672 ± 0.624
3.619LeuPro: 3.619 ± 0.379
2.172LeuGln: 2.172 ± 0.388
4.541LeuArg: 4.541 ± 0.492
5.067LeuSer: 5.067 ± 0.496
6.449LeuThr: 6.449 ± 0.703
5.001LeuVal: 5.001 ± 0.524
0.724LeuTrp: 0.724 ± 0.31
2.369LeuTyr: 2.369 ± 0.323
0.0LeuXaa: 0.0 ± 0.0
Met
2.501MetAla: 2.501 ± 0.4
0.066MetCys: 0.066 ± 0.076
1.053MetAsp: 1.053 ± 0.194
1.316MetGlu: 1.316 ± 0.279
0.921MetPhe: 0.921 ± 0.205
1.777MetGly: 1.777 ± 0.311
0.526MetHis: 0.526 ± 0.181
1.645MetIle: 1.645 ± 0.414
1.777MetLys: 1.777 ± 0.295
2.501MetLeu: 2.501 ± 0.439
0.79MetMet: 0.79 ± 0.218
1.119MetAsn: 1.119 ± 0.262
1.053MetPro: 1.053 ± 0.237
0.855MetGln: 0.855 ± 0.201
1.908MetArg: 1.908 ± 0.305
1.974MetSer: 1.974 ± 0.339
2.83MetThr: 2.83 ± 0.324
0.329MetVal: 0.329 ± 0.14
0.132MetTrp: 0.132 ± 0.094
0.658MetTyr: 0.658 ± 0.182
0.0MetXaa: 0.0 ± 0.0
Asn
5.067AsnAla: 5.067 ± 0.605
0.263AsnCys: 0.263 ± 0.133
2.83AsnAsp: 2.83 ± 0.406
2.501AsnGlu: 2.501 ± 0.484
1.053AsnPhe: 1.053 ± 0.242
3.883AsnGly: 3.883 ± 0.462
1.119AsnHis: 1.119 ± 0.277
2.83AsnIle: 2.83 ± 0.431
2.106AsnLys: 2.106 ± 0.381
4.014AsnLeu: 4.014 ± 0.45
0.855AsnMet: 0.855 ± 0.251
2.435AsnAsn: 2.435 ± 0.385
3.159AsnPro: 3.159 ± 0.537
1.711AsnGln: 1.711 ± 0.353
2.566AsnArg: 2.566 ± 0.339
3.356AsnSer: 3.356 ± 0.546
2.303AsnThr: 2.303 ± 0.382
3.093AsnVal: 3.093 ± 0.369
0.263AsnTrp: 0.263 ± 0.138
1.777AsnTyr: 1.777 ± 0.324
0.0AsnXaa: 0.0 ± 0.0
Pro
5.33ProAla: 5.33 ± 0.753
0.395ProCys: 0.395 ± 0.157
3.685ProAsp: 3.685 ± 0.405
3.159ProGlu: 3.159 ± 0.394
1.448ProPhe: 1.448 ± 0.344
2.895ProGly: 2.895 ± 0.329
1.185ProHis: 1.185 ± 0.268
2.566ProIle: 2.566 ± 0.508
1.514ProLys: 1.514 ± 0.324
2.764ProLeu: 2.764 ± 0.395
0.855ProMet: 0.855 ± 0.235
1.185ProAsn: 1.185 ± 0.277
1.974ProPro: 1.974 ± 0.354
1.053ProGln: 1.053 ± 0.23
2.303ProArg: 2.303 ± 0.434
2.632ProSer: 2.632 ± 0.39
2.764ProThr: 2.764 ± 0.364
5.396ProVal: 5.396 ± 0.697
0.658ProTrp: 0.658 ± 0.201
0.921ProTyr: 0.921 ± 0.293
0.0ProXaa: 0.0 ± 0.0
Gln
4.936GlnAla: 4.936 ± 0.531
0.592GlnCys: 0.592 ± 0.18
1.777GlnAsp: 1.777 ± 0.399
1.843GlnGlu: 1.843 ± 0.352
1.777GlnPhe: 1.777 ± 0.346
2.237GlnGly: 2.237 ± 0.341
0.461GlnHis: 0.461 ± 0.152
2.698GlnIle: 2.698 ± 0.419
2.04GlnLys: 2.04 ± 0.373
3.554GlnLeu: 3.554 ± 0.513
1.053GlnMet: 1.053 ± 0.252
1.382GlnAsn: 1.382 ± 0.331
2.172GlnPro: 2.172 ± 0.428
2.435GlnGln: 2.435 ± 0.323
2.895GlnArg: 2.895 ± 0.435
1.908GlnSer: 1.908 ± 0.338
1.843GlnThr: 1.843 ± 0.348
2.435GlnVal: 2.435 ± 0.381
0.461GlnTrp: 0.461 ± 0.235
1.382GlnTyr: 1.382 ± 0.215
0.0GlnXaa: 0.0 ± 0.0
Arg
4.804ArgAla: 4.804 ± 0.502
0.592ArgCys: 0.592 ± 0.179
4.08ArgAsp: 4.08 ± 0.557
3.29ArgGlu: 3.29 ± 0.499
1.382ArgPhe: 1.382 ± 0.254
3.619ArgGly: 3.619 ± 0.516
0.658ArgHis: 0.658 ± 0.205
3.356ArgIle: 3.356 ± 0.493
3.619ArgLys: 3.619 ± 0.579
5.462ArgLeu: 5.462 ± 0.636
1.777ArgMet: 1.777 ± 0.322
2.566ArgAsn: 2.566 ± 0.426
3.027ArgPro: 3.027 ± 0.483
2.764ArgGln: 2.764 ± 0.435
4.87ArgArg: 4.87 ± 0.645
3.554ArgSer: 3.554 ± 0.471
3.093ArgThr: 3.093 ± 0.467
4.014ArgVal: 4.014 ± 0.579
1.645ArgTrp: 1.645 ± 0.32
2.303ArgTyr: 2.303 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
7.436SerAla: 7.436 ± 0.918
0.855SerCys: 0.855 ± 0.181
5.133SerAsp: 5.133 ± 0.496
3.619SerGlu: 3.619 ± 0.541
2.303SerPhe: 2.303 ± 0.381
7.041SerGly: 7.041 ± 0.752
0.461SerHis: 0.461 ± 0.146
3.422SerIle: 3.422 ± 0.439
2.237SerLys: 2.237 ± 0.321
5.265SerLeu: 5.265 ± 0.616
1.316SerMet: 1.316 ± 0.269
2.566SerAsn: 2.566 ± 0.5
2.632SerPro: 2.632 ± 0.429
2.501SerGln: 2.501 ± 0.394
3.027SerArg: 3.027 ± 0.446
5.199SerSer: 5.199 ± 0.941
4.146SerThr: 4.146 ± 0.631
6.12SerVal: 6.12 ± 0.753
1.053SerTrp: 1.053 ± 0.299
1.711SerTyr: 1.711 ± 0.327
0.0SerXaa: 0.0 ± 0.0
Thr
7.107ThrAla: 7.107 ± 0.962
0.855ThrCys: 0.855 ± 0.226
4.672ThrAsp: 4.672 ± 0.505
3.225ThrGlu: 3.225 ± 0.537
2.501ThrPhe: 2.501 ± 0.456
5.396ThrGly: 5.396 ± 0.412
0.921ThrHis: 0.921 ± 0.197
3.883ThrIle: 3.883 ± 0.438
2.237ThrLys: 2.237 ± 0.374
5.067ThrLeu: 5.067 ± 0.644
0.855ThrMet: 0.855 ± 0.211
2.106ThrAsn: 2.106 ± 0.276
4.212ThrPro: 4.212 ± 0.385
3.027ThrGln: 3.027 ± 0.53
4.08ThrArg: 4.08 ± 0.437
4.804ThrSer: 4.804 ± 0.607
3.685ThrThr: 3.685 ± 0.54
4.409ThrVal: 4.409 ± 0.56
0.592ThrTrp: 0.592 ± 0.18
1.185ThrTyr: 1.185 ± 0.251
0.0ThrXaa: 0.0 ± 0.0
Val
6.317ValAla: 6.317 ± 0.483
0.461ValCys: 0.461 ± 0.139
4.409ValAsp: 4.409 ± 0.487
3.751ValGlu: 3.751 ± 0.411
2.106ValPhe: 2.106 ± 0.379
3.751ValGly: 3.751 ± 0.528
0.724ValHis: 0.724 ± 0.183
4.014ValIle: 4.014 ± 0.521
3.027ValLys: 3.027 ± 0.407
4.541ValLeu: 4.541 ± 0.555
1.514ValMet: 1.514 ± 0.295
3.883ValAsn: 3.883 ± 0.541
2.764ValPro: 2.764 ± 0.505
2.764ValGln: 2.764 ± 0.324
4.08ValArg: 4.08 ± 0.445
5.528ValSer: 5.528 ± 0.677
5.199ValThr: 5.199 ± 0.554
4.804ValVal: 4.804 ± 0.643
0.592ValTrp: 0.592 ± 0.175
1.777ValTyr: 1.777 ± 0.299
0.0ValXaa: 0.0 ± 0.0
Trp
0.987TrpAla: 0.987 ± 0.24
0.263TrpCys: 0.263 ± 0.123
0.592TrpAsp: 0.592 ± 0.204
1.053TrpGlu: 1.053 ± 0.313
0.724TrpPhe: 0.724 ± 0.231
0.855TrpGly: 0.855 ± 0.182
0.329TrpHis: 0.329 ± 0.136
0.724TrpIle: 0.724 ± 0.234
0.724TrpLys: 0.724 ± 0.21
1.119TrpLeu: 1.119 ± 0.237
0.395TrpMet: 0.395 ± 0.149
0.658TrpAsn: 0.658 ± 0.189
0.658TrpPro: 0.658 ± 0.209
0.724TrpGln: 0.724 ± 0.209
1.448TrpArg: 1.448 ± 0.31
1.053TrpSer: 1.053 ± 0.246
0.658TrpThr: 0.658 ± 0.159
0.921TrpVal: 0.921 ± 0.214
0.132TrpTrp: 0.132 ± 0.131
0.395TrpTyr: 0.395 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.369TyrAla: 2.369 ± 0.412
0.329TyrCys: 0.329 ± 0.137
1.382TyrAsp: 1.382 ± 0.305
1.25TyrGlu: 1.25 ± 0.279
1.185TyrPhe: 1.185 ± 0.274
1.974TyrGly: 1.974 ± 0.386
0.526TyrHis: 0.526 ± 0.144
1.514TyrIle: 1.514 ± 0.306
0.855TyrLys: 0.855 ± 0.213
2.764TyrLeu: 2.764 ± 0.393
0.79TyrMet: 0.79 ± 0.243
2.237TyrAsn: 2.237 ± 0.359
1.382TyrPro: 1.382 ± 0.281
1.053TyrGln: 1.053 ± 0.239
2.172TyrArg: 2.172 ± 0.333
1.843TyrSer: 1.843 ± 0.302
2.83TyrThr: 2.83 ± 0.385
2.106TyrVal: 2.106 ± 0.361
0.526TyrTrp: 0.526 ± 0.188
0.855TyrTyr: 0.855 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (15197 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski