Amino acid dipepetide frequency for Enterobacteriaceae bacterium ENNIH1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.342AlaAla: 10.342 ± 0.123	1.177AlaCys: 1.177 ± 0.03	5.021AlaAsp: 5.021 ± 0.056	5.633AlaGlu: 5.633 ± 0.064	3.781AlaPhe: 3.781 ± 0.049	7.663AlaGly: 7.663 ± 0.078	2.006AlaHis: 2.006 ± 0.038	5.907AlaIle: 5.907 ± 0.063	3.867AlaLys: 3.867 ± 0.059	12.066AlaLeu: 12.066 ± 0.114	3.002AlaMet: 3.002 ± 0.048	3.01AlaAsn: 3.01 ± 0.041	3.7AlaPro: 3.7 ± 0.057	4.579AlaGln: 4.579 ± 0.061	5.83AlaArg: 5.83 ± 0.07	5.767AlaSer: 5.767 ± 0.064	4.958AlaThr: 4.958 ± 0.063	6.817AlaVal: 6.817 ± 0.075	1.672AlaTrp: 1.672 ± 0.036	1.952AlaTyr: 1.952 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.045CysAla: 1.045 ± 0.03	0.18CysCys: 0.18 ± 0.01	0.631CysAsp: 0.631 ± 0.023	0.656CysGlu: 0.656 ± 0.022	0.483CysPhe: 0.483 ± 0.018	1.063CysGly: 1.063 ± 0.029	0.336CysHis: 0.336 ± 0.013	0.566CysIle: 0.566 ± 0.018	0.338CysLys: 0.338 ± 0.015	1.077CysLeu: 1.077 ± 0.028	0.263CysMet: 0.263 ± 0.013	0.349CysAsn: 0.349 ± 0.017	0.55CysPro: 0.55 ± 0.021	0.447CysGln: 0.447 ± 0.019	0.662CysArg: 0.662 ± 0.022	0.674CysSer: 0.674 ± 0.021	0.483CysThr: 0.483 ± 0.018	0.791CysVal: 0.791 ± 0.024	0.183CysTrp: 0.183 ± 0.011	0.346CysTyr: 0.346 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.414AspAla: 5.414 ± 0.067	0.515AspCys: 0.515 ± 0.019	2.996AspAsp: 2.996 ± 0.056	3.704AspGlu: 3.704 ± 0.049	2.119AspPhe: 2.119 ± 0.038	3.717AspGly: 3.717 ± 0.052	1.007AspHis: 1.007 ± 0.026	3.504AspIle: 3.504 ± 0.045	2.533AspLys: 2.533 ± 0.043	4.69AspLeu: 4.69 ± 0.065	1.342AspMet: 1.342 ± 0.031	2.209AspAsn: 2.209 ± 0.041	2.244AspPro: 2.244 ± 0.039	1.568AspGln: 1.568 ± 0.032	2.626AspArg: 2.626 ± 0.041	2.747AspSer: 2.747 ± 0.045	2.602AspThr: 2.602 ± 0.043	3.927AspVal: 3.927 ± 0.054	0.812AspTrp: 0.812 ± 0.023	1.826AspTyr: 1.826 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	5.389GluAla: 5.389 ± 0.061	0.498GluCys: 0.498 ± 0.022	2.421GluAsp: 2.421 ± 0.047	3.442GluGlu: 3.442 ± 0.056	1.86GluPhe: 1.86 ± 0.036	3.682GluGly: 3.682 ± 0.046	1.436GluHis: 1.436 ± 0.032	3.313GluIle: 3.313 ± 0.054	3.302GluLys: 3.302 ± 0.052	5.935GluLeu: 5.935 ± 0.067	1.867GluMet: 1.867 ± 0.037	2.42GluAsn: 2.42 ± 0.044	2.091GluPro: 2.091 ± 0.043	3.332GluGln: 3.332 ± 0.056	3.711GluArg: 3.711 ± 0.051	3.059GluSer: 3.059 ± 0.041	2.992GluThr: 2.992 ± 0.046	3.715GluVal: 3.715 ± 0.054	0.793GluTrp: 0.793 ± 0.027	1.46GluTyr: 1.46 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.833PheAla: 3.833 ± 0.058	0.572PheCys: 0.572 ± 0.019	2.394PheAsp: 2.394 ± 0.043	1.789PheGlu: 1.789 ± 0.035	1.729PhePhe: 1.729 ± 0.039	3.166PheGly: 3.166 ± 0.046	0.836PheHis: 0.836 ± 0.024	2.641PheIle: 2.641 ± 0.043	1.284PheLys: 1.284 ± 0.029	3.392PheLeu: 3.392 ± 0.058	1.0PheMet: 1.0 ± 0.026	1.705PheAsn: 1.705 ± 0.034	1.604PhePro: 1.604 ± 0.032	1.117PheGln: 1.117 ± 0.028	2.036PheArg: 2.036 ± 0.04	3.086PheSer: 3.086 ± 0.044	2.441PheThr: 2.441 ± 0.038	2.54PheVal: 2.54 ± 0.045	0.655PheTrp: 0.655 ± 0.022	1.197PheTyr: 1.197 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	6.268GlyAla: 6.268 ± 0.079	1.004GlyCys: 1.004 ± 0.026	3.71GlyAsp: 3.71 ± 0.045	4.557GlyGlu: 4.557 ± 0.057	3.257GlyPhe: 3.257 ± 0.047	5.373GlyGly: 5.373 ± 0.067	1.636GlyHis: 1.636 ± 0.035	5.063GlyIle: 5.063 ± 0.068	4.0GlyLys: 4.0 ± 0.055	7.554GlyLeu: 7.554 ± 0.075	2.404GlyMet: 2.404 ± 0.036	2.639GlyAsn: 2.639 ± 0.042	2.063GlyPro: 2.063 ± 0.041	2.921GlyGln: 2.921 ± 0.048	3.786GlyArg: 3.786 ± 0.053	4.158GlySer: 4.158 ± 0.057	3.701GlyThr: 3.701 ± 0.049	5.71GlyVal: 5.71 ± 0.056	1.271GlyTrp: 1.271 ± 0.032	2.563GlyTyr: 2.563 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.025HisAla: 2.025 ± 0.043	0.329HisCys: 0.329 ± 0.016	1.199HisAsp: 1.199 ± 0.034	1.156HisGlu: 1.156 ± 0.026	1.082HisPhe: 1.082 ± 0.026	1.716HisGly: 1.716 ± 0.032	0.789HisHis: 0.789 ± 0.027	1.391HisIle: 1.391 ± 0.028	0.766HisLys: 0.766 ± 0.022	2.349HisLeu: 2.349 ± 0.04	0.554HisMet: 0.554 ± 0.017	0.835HisAsn: 0.835 ± 0.022	1.338HisPro: 1.338 ± 0.033	1.112HisGln: 1.112 ± 0.029	1.276HisArg: 1.276 ± 0.029	1.327HisSer: 1.327 ± 0.028	1.091HisThr: 1.091 ± 0.027	1.354HisVal: 1.354 ± 0.033	0.4HisTrp: 0.4 ± 0.016	0.901HisTyr: 0.901 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.478IleAla: 6.478 ± 0.079	0.701IleCys: 0.701 ± 0.024	3.518IleAsp: 3.518 ± 0.055	3.338IleGlu: 3.338 ± 0.051	2.037IlePhe: 2.037 ± 0.041	4.487IleGly: 4.487 ± 0.066	1.197IleHis: 1.197 ± 0.027	3.423IleIle: 3.423 ± 0.059	2.29IleLys: 2.29 ± 0.041	5.014IleLeu: 5.014 ± 0.077	1.248IleMet: 1.248 ± 0.032	2.608IleAsn: 2.608 ± 0.042	2.727IlePro: 2.727 ± 0.04	1.849IleGln: 1.849 ± 0.036	3.103IleArg: 3.103 ± 0.043	3.922IleSer: 3.922 ± 0.053	3.554IleThr: 3.554 ± 0.047	3.963IleVal: 3.963 ± 0.051	0.719IleTrp: 0.719 ± 0.021	1.495IleTyr: 1.495 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.373LysAla: 4.373 ± 0.073	0.3LysCys: 0.3 ± 0.016	2.099LysAsp: 2.099 ± 0.046	2.508LysGlu: 2.508 ± 0.046	1.156LysPhe: 1.156 ± 0.026	2.958LysGly: 2.958 ± 0.053	0.879LysHis: 0.879 ± 0.023	2.363LysIle: 2.363 ± 0.039	2.304LysLys: 2.304 ± 0.046	4.222LysLeu: 4.222 ± 0.053	1.262LysMet: 1.262 ± 0.03	1.757LysAsn: 1.757 ± 0.035	2.126LysPro: 2.126 ± 0.039	1.895LysGln: 1.895 ± 0.043	2.6LysArg: 2.6 ± 0.04	2.363LysSer: 2.363 ± 0.036	2.625LysThr: 2.625 ± 0.043	2.981LysVal: 2.981 ± 0.051	0.444LysTrp: 0.444 ± 0.017	1.112LysTyr: 1.112 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	11.646LeuAla: 11.646 ± 0.124	1.325LeuCys: 1.325 ± 0.031	5.382LeuAsp: 5.382 ± 0.057	5.556LeuGlu: 5.556 ± 0.074	4.3LeuPhe: 4.3 ± 0.066	7.201LeuGly: 7.201 ± 0.081	2.357LeuHis: 2.357 ± 0.036	5.822LeuIle: 5.822 ± 0.071	4.553LeuLys: 4.553 ± 0.057	11.843LeuLeu: 11.843 ± 0.126	2.942LeuMet: 2.942 ± 0.049	4.181LeuAsn: 4.181 ± 0.046	5.678LeuPro: 5.678 ± 0.069	4.013LeuGln: 4.013 ± 0.046	6.364LeuArg: 6.364 ± 0.062	7.31LeuSer: 7.31 ± 0.075	6.414LeuThr: 6.414 ± 0.06	7.16LeuVal: 7.16 ± 0.074	1.434LeuTrp: 1.434 ± 0.029	2.591LeuTyr: 2.591 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.931MetAla: 2.931 ± 0.042	0.208MetCys: 0.208 ± 0.011	1.214MetAsp: 1.214 ± 0.03	1.283MetGlu: 1.283 ± 0.032	0.878MetPhe: 0.878 ± 0.022	1.887MetGly: 1.887 ± 0.033	0.563MetHis: 0.563 ± 0.018	1.458MetIle: 1.458 ± 0.032	1.43MetLys: 1.43 ± 0.028	3.158MetLeu: 3.158 ± 0.046	0.889MetMet: 0.889 ± 0.026	1.109MetAsn: 1.109 ± 0.03	1.332MetPro: 1.332 ± 0.031	1.2MetGln: 1.2 ± 0.028	1.538MetArg: 1.538 ± 0.03	1.981MetSer: 1.981 ± 0.038	1.843MetThr: 1.843 ± 0.027	1.908MetVal: 1.908 ± 0.039	0.253MetTrp: 0.253 ± 0.013	0.515MetTyr: 0.515 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.678AsnAla: 3.678 ± 0.05	0.334AsnCys: 0.334 ± 0.015	2.04AsnAsp: 2.04 ± 0.037	2.01AsnGlu: 2.01 ± 0.039	1.329AsnPhe: 1.329 ± 0.031	2.964AsnGly: 2.964 ± 0.049	0.837AsnHis: 0.837 ± 0.025	2.337AsnIle: 2.337 ± 0.039	1.554AsnLys: 1.554 ± 0.032	3.459AsnLeu: 3.459 ± 0.048	0.915AsnMet: 0.915 ± 0.024	1.546AsnAsn: 1.546 ± 0.035	2.106AsnPro: 2.106 ± 0.043	1.507AsnGln: 1.507 ± 0.032	1.998AsnArg: 1.998 ± 0.035	1.946AsnSer: 1.946 ± 0.039	2.035AsnThr: 2.035 ± 0.041	2.613AsnVal: 2.613 ± 0.047	0.558AsnTrp: 0.558 ± 0.02	1.115AsnTyr: 1.115 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	4.577ProAla: 4.577 ± 0.061	0.41ProCys: 0.41 ± 0.018	2.882ProAsp: 2.882 ± 0.045	3.321ProGlu: 3.321 ± 0.046	1.856ProPhe: 1.856 ± 0.037	3.623ProGly: 3.623 ± 0.054	1.071ProHis: 1.071 ± 0.025	1.759ProIle: 1.759 ± 0.033	1.516ProLys: 1.516 ± 0.032	5.057ProLeu: 5.057 ± 0.061	1.094ProMet: 1.094 ± 0.028	1.219ProAsn: 1.219 ± 0.027	1.661ProPro: 1.661 ± 0.039	2.225ProGln: 2.225 ± 0.041	2.016ProArg: 2.016 ± 0.036	2.232ProSer: 2.232 ± 0.043	2.083ProThr: 2.083 ± 0.036	3.988ProVal: 3.988 ± 0.053	0.772ProTrp: 0.772 ± 0.024	1.145ProTyr: 1.145 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.638GlnAla: 4.638 ± 0.061	0.428GlnCys: 0.428 ± 0.016	1.803GlnAsp: 1.803 ± 0.033	2.25GlnGlu: 2.25 ± 0.039	1.465GlnPhe: 1.465 ± 0.027	3.006GlnGly: 3.006 ± 0.045	1.173GlnHis: 1.173 ± 0.029	2.292GlnIle: 2.292 ± 0.044	1.851GlnLys: 1.851 ± 0.042	4.786GlnLeu: 4.786 ± 0.061	1.22GlnMet: 1.22 ± 0.029	1.459GlnAsn: 1.459 ± 0.028	2.09GlnPro: 2.09 ± 0.043	3.034GlnGln: 3.034 ± 0.06	3.024GlnArg: 3.024 ± 0.052	2.374GlnSer: 2.374 ± 0.043	2.315GlnThr: 2.315 ± 0.045	2.811GlnVal: 2.811 ± 0.045	0.657GlnTrp: 0.657 ± 0.02	1.205GlnTyr: 1.205 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	4.716ArgAla: 4.716 ± 0.059	0.603ArgCys: 0.603 ± 0.02	3.064ArgAsp: 3.064 ± 0.05	3.855ArgGlu: 3.855 ± 0.044	2.604ArgPhe: 2.604 ± 0.037	3.442ArgGly: 3.442 ± 0.046	1.657ArgHis: 1.657 ± 0.035	3.34ArgIle: 3.34 ± 0.051	2.449ArgLys: 2.449 ± 0.035	6.63ArgLeu: 6.63 ± 0.069	1.622ArgMet: 1.622 ± 0.028	2.09ArgAsn: 2.09 ± 0.04	2.238ArgPro: 2.238 ± 0.039	3.132ArgGln: 3.132 ± 0.052	3.608ArgArg: 3.608 ± 0.052	2.86ArgSer: 2.86 ± 0.045	2.663ArgThr: 2.663 ± 0.038	3.995ArgVal: 3.995 ± 0.053	0.985ArgTrp: 0.985 ± 0.028	2.175ArgTyr: 2.175 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	5.76SerAla: 5.76 ± 0.068	0.598SerCys: 0.598 ± 0.024	3.16SerAsp: 3.16 ± 0.046	3.256SerGlu: 3.256 ± 0.058	2.347SerPhe: 2.347 ± 0.036	5.265SerGly: 5.265 ± 0.061	1.469SerHis: 1.469 ± 0.034	2.965SerIle: 2.965 ± 0.046	2.124SerLys: 2.124 ± 0.044	6.745SerLeu: 6.745 ± 0.075	1.53SerMet: 1.53 ± 0.033	1.831SerAsn: 1.831 ± 0.037	2.662SerPro: 2.662 ± 0.044	2.63SerGln: 2.63 ± 0.047	3.584SerArg: 3.584 ± 0.044	3.63SerSer: 3.63 ± 0.058	3.045SerThr: 3.045 ± 0.05	4.369SerVal: 4.369 ± 0.051	0.959SerTrp: 0.959 ± 0.026	1.601SerTyr: 1.601 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	5.073ThrAla: 5.073 ± 0.06	0.539ThrCys: 0.539 ± 0.019	2.714ThrAsp: 2.714 ± 0.043	2.683ThrGlu: 2.683 ± 0.043	2.145ThrPhe: 2.145 ± 0.036	4.601ThrGly: 4.601 ± 0.053	1.257ThrHis: 1.257 ± 0.028	2.835ThrIle: 2.835 ± 0.043	1.551ThrLys: 1.551 ± 0.037	7.514ThrLeu: 7.514 ± 0.074	1.213ThrMet: 1.213 ± 0.026	1.56ThrAsn: 1.56 ± 0.037	3.249ThrPro: 3.249 ± 0.054	2.257ThrGln: 2.257 ± 0.039	3.158ThrArg: 3.158 ± 0.048	2.944ThrSer: 2.944 ± 0.043	2.995ThrThr: 2.995 ± 0.053	3.951ThrVal: 3.951 ± 0.055	0.831ThrTrp: 0.831 ± 0.023	1.15ThrTyr: 1.15 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	7.083ValAla: 7.083 ± 0.081	0.814ValCys: 0.814 ± 0.024	3.658ValAsp: 3.658 ± 0.052	3.875ValGlu: 3.875 ± 0.057	2.693ValPhe: 2.693 ± 0.041	4.745ValGly: 4.745 ± 0.068	1.303ValHis: 1.303 ± 0.027	4.604ValIle: 4.604 ± 0.068	3.132ValLys: 3.132 ± 0.052	7.422ValLeu: 7.422 ± 0.074	2.184ValMet: 2.184 ± 0.033	2.845ValAsn: 2.845 ± 0.045	3.085ValPro: 3.085 ± 0.05	2.376ValGln: 2.376 ± 0.041	3.804ValArg: 3.804 ± 0.053	4.691ValSer: 4.691 ± 0.054	4.294ValThr: 4.294 ± 0.055	5.604ValVal: 5.604 ± 0.072	1.007ValTrp: 1.007 ± 0.028	1.656ValTyr: 1.656 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.009TrpAla: 1.009 ± 0.024	0.21TrpCys: 0.21 ± 0.012	0.63TrpAsp: 0.63 ± 0.019	0.619TrpGlu: 0.619 ± 0.019	0.733TrpPhe: 0.733 ± 0.019	0.921TrpGly: 0.921 ± 0.023	0.489TrpHis: 0.489 ± 0.02	0.71TrpIle: 0.71 ± 0.023	0.537TrpLys: 0.537 ± 0.018	2.288TrpLeu: 2.288 ± 0.044	0.429TrpMet: 0.429 ± 0.018	0.498TrpAsn: 0.498 ± 0.017	0.666TrpPro: 0.666 ± 0.023	1.165TrpGln: 1.165 ± 0.032	1.107TrpArg: 1.107 ± 0.028	0.785TrpSer: 0.785 ± 0.025	0.621TrpThr: 0.621 ± 0.022	0.958TrpVal: 0.958 ± 0.024	0.253TrpTrp: 0.253 ± 0.013	0.449TrpTyr: 0.449 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.47TyrAla: 2.47 ± 0.038	0.377TyrCys: 0.377 ± 0.016	1.571TyrAsp: 1.571 ± 0.034	1.227TyrGlu: 1.227 ± 0.026	1.165TyrPhe: 1.165 ± 0.033	2.157TyrGly: 2.157 ± 0.04	0.692TyrHis: 0.692 ± 0.023	1.379TyrIle: 1.379 ± 0.033	0.948TyrLys: 0.948 ± 0.026	2.873TyrLeu: 2.873 ± 0.041	0.596TyrMet: 0.596 ± 0.018	1.012TyrAsn: 1.012 ± 0.026	1.295TyrPro: 1.295 ± 0.028	1.519TyrGln: 1.519 ± 0.033	1.897TyrArg: 1.897 ± 0.036	1.673TyrSer: 1.673 ± 0.037	1.463TyrThr: 1.463 ± 0.039	1.72TyrVal: 1.72 ± 0.035	0.42TyrTrp: 0.42 ± 0.016	0.86TyrTyr: 0.86 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5131 proteins (1564357 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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