Lisianthus necrosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus; Eggplant mottled crinkle virus

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7M8Y5|A7M8Y5_9TOMB Uncharacterized protein OS=Lisianthus necrosis virus OX=334425 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 5.14RR3 pKa = 11.84AIQGSDD9 pKa = 3.0ARR11 pKa = 11.84EE12 pKa = 3.66QAYY15 pKa = 9.45SEE17 pKa = 4.32RR18 pKa = 11.84WDD20 pKa = 3.91GGCGGTITPFKK31 pKa = 10.79LPDD34 pKa = 3.68EE35 pKa = 4.67SPSLIEE41 pKa = 3.82WRR43 pKa = 11.84LHH45 pKa = 5.17NSEE48 pKa = 4.22EE49 pKa = 5.29SEE51 pKa = 5.27DD52 pKa = 3.69KK53 pKa = 11.15DD54 pKa = 3.57NPLGFKK60 pKa = 10.18EE61 pKa = 4.13SWSFGKK67 pKa = 10.55VVFKK71 pKa = 10.76RR72 pKa = 11.84YY73 pKa = 9.63LRR75 pKa = 11.84YY76 pKa = 10.51DD77 pKa = 3.22GTEE80 pKa = 3.86ASLHH84 pKa = 5.89RR85 pKa = 11.84ALGSWEE91 pKa = 4.03RR92 pKa = 11.84DD93 pKa = 3.57TVNDD97 pKa = 3.45AASRR101 pKa = 11.84FLGFGQIGCTYY112 pKa = 9.88CIRR115 pKa = 11.84FRR117 pKa = 11.84GSCLTISGGSRR128 pKa = 11.84TLQRR132 pKa = 11.84LIEE135 pKa = 3.89MAIRR139 pKa = 11.84TKK141 pKa = 10.37RR142 pKa = 11.84TMLQLAPCEE151 pKa = 4.22VEE153 pKa = 4.75GNVSRR158 pKa = 11.84GSPEE162 pKa = 3.35GTEE165 pKa = 3.95AFKK168 pKa = 11.2EE169 pKa = 4.15EE170 pKa = 4.46SEE172 pKa = 4.25

Molecular weight: 19.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7M8Y1|A7M8Y1_9TOMB Isoform of A7M8Y0 RNA-directed RNA polymerase (Fragment) OS=Lisianthus necrosis virus OX=334425 GN=replicase PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 5.53RR3 pKa = 11.84LLNFLRR9 pKa = 11.84PKK11 pKa = 10.64KK12 pKa = 10.19EE13 pKa = 3.6IFVGDD18 pKa = 3.45FAIGVDD24 pKa = 4.11RR25 pKa = 11.84RR26 pKa = 11.84PSMDD30 pKa = 3.08MFQLVCRR37 pKa = 11.84LALRR41 pKa = 11.84YY42 pKa = 8.41MRR44 pKa = 11.84TGKK47 pKa = 9.76IEE49 pKa = 4.44CNVDD53 pKa = 3.29SLSQFVVEE61 pKa = 5.75LLKK64 pKa = 10.18TDD66 pKa = 5.11CAAKK70 pKa = 10.03WEE72 pKa = 4.24WFMKK76 pKa = 10.02RR77 pKa = 11.84RR78 pKa = 11.84SAGDD82 pKa = 3.25YY83 pKa = 10.33AIPLALAALPIAPLLGYY100 pKa = 7.82ATKK103 pKa = 10.64VRR105 pKa = 11.84TVSVKK110 pKa = 10.79AFGNEE115 pKa = 3.4LTFPFRR121 pKa = 11.84TLRR124 pKa = 11.84PSFPRR129 pKa = 11.84KK130 pKa = 9.85GLLLRR135 pKa = 11.84LAAGLALAPVCALAVYY151 pKa = 7.97ATLPRR156 pKa = 11.84EE157 pKa = 3.94KK158 pKa = 10.69LSVFRR163 pKa = 11.84LRR165 pKa = 11.84TEE167 pKa = 3.98ARR169 pKa = 11.84THH171 pKa = 5.86MEE173 pKa = 4.19DD174 pKa = 3.06EE175 pKa = 4.51RR176 pKa = 11.84EE177 pKa = 4.18ATEE180 pKa = 4.15CLVVEE185 pKa = 4.99PARR188 pKa = 11.84EE189 pKa = 3.99LKK191 pKa = 10.99GKK193 pKa = 10.26DD194 pKa = 3.6GEE196 pKa = 4.5DD197 pKa = 3.55LLTGSRR203 pKa = 11.84MTKK206 pKa = 10.3VIASTGRR213 pKa = 11.84PRR215 pKa = 11.84RR216 pKa = 11.84RR217 pKa = 11.84PYY219 pKa = 9.5AAKK222 pKa = 9.19VAQVARR228 pKa = 11.84AKK230 pKa = 10.53VGYY233 pKa = 9.9LKK235 pKa = 10.75NSPEE239 pKa = 3.62NRR241 pKa = 11.84LIYY244 pKa = 10.08QRR246 pKa = 11.84VIIEE250 pKa = 4.93IMDD253 pKa = 4.1KK254 pKa = 11.13DD255 pKa = 3.69CVRR258 pKa = 11.84YY259 pKa = 9.97VDD261 pKa = 4.33RR262 pKa = 11.84DD263 pKa = 3.8VILPLAIGCCFVYY276 pKa = 10.38PDD278 pKa = 4.17GVEE281 pKa = 3.91EE282 pKa = 5.03SAALWGSSEE291 pKa = 4.13SLGVKK296 pKa = 9.68

Molecular weight: 33.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

1

6

1903

39

817

317.2

35.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.04 ± 0.858

2.207 ± 0.339

4.467 ± 0.299

6.043 ± 0.932

3.941 ± 0.206

7.935 ± 0.662

1.576 ± 0.453

3.941 ± 0.086

5.465 ± 0.643

10.194 ± 0.676

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.522 ± 0.127

3.573 ± 0.966

4.782 ± 0.255

2.522 ± 0.239

7.725 ± 0.79

6.463 ± 0.84

6.148 ± 1.158

8.04 ± 0.663

1.261 ± 0.172

3.153 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski