Streptomyces phage Comrade

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gilsonvirus; Streptomyces virus Comrade

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DV19|A0A385DV19_9CAUD dUTP diphosphatase OS=Streptomyces phage Comrade OX=2301714 GN=63 PE=3 SV=1
MM1 pKa = 7.76ADD3 pKa = 2.88IVFPGMAAGGPLGQDD18 pKa = 3.54YY19 pKa = 11.19LPANYY24 pKa = 10.45DD25 pKa = 3.45LVLYY29 pKa = 10.42KK30 pKa = 10.57GDD32 pKa = 4.0YY33 pKa = 10.77FSMSLTFKK41 pKa = 11.04NPDD44 pKa = 3.63NSPMDD49 pKa = 3.59LTGYY53 pKa = 7.12TAQCSIRR60 pKa = 11.84ATVGASEE67 pKa = 4.66GFDD70 pKa = 3.45AEE72 pKa = 4.4LTITPLQGKK81 pKa = 9.57VDD83 pKa = 3.75VLFPSSVTSTLTAGDD98 pKa = 4.26YY99 pKa = 10.97VWDD102 pKa = 4.38FQLTNPDD109 pKa = 3.48DD110 pKa = 3.83NVRR113 pKa = 11.84TFFAGDD119 pKa = 3.21VKK121 pKa = 11.22VYY123 pKa = 11.29GEE125 pKa = 4.04ITSS128 pKa = 3.49

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385DVH2|A0A385DVH2_9CAUD Uncharacterized protein OS=Streptomyces phage Comrade OX=2301714 GN=252 PE=4 SV=1
MM1 pKa = 7.86FEE3 pKa = 4.35FTPEE7 pKa = 3.66YY8 pKa = 10.35KK9 pKa = 10.16RR10 pKa = 11.84YY11 pKa = 9.76IEE13 pKa = 3.93SDD15 pKa = 2.68RR16 pKa = 11.84WKK18 pKa = 10.49LVCARR23 pKa = 11.84YY24 pKa = 8.02WAVYY28 pKa = 9.43GKK30 pKa = 10.05KK31 pKa = 9.99CQACGSRR38 pKa = 11.84KK39 pKa = 9.31NLHH42 pKa = 5.16VHH44 pKa = 6.02HH45 pKa = 6.37NTYY48 pKa = 10.51EE49 pKa = 4.12RR50 pKa = 11.84FGRR53 pKa = 11.84EE54 pKa = 3.64VLKK57 pKa = 10.94DD58 pKa = 3.43LTGVCQACHH67 pKa = 6.43RR68 pKa = 11.84EE69 pKa = 3.51IHH71 pKa = 6.0RR72 pKa = 11.84LHH74 pKa = 6.49RR75 pKa = 11.84RR76 pKa = 11.84DD77 pKa = 3.1RR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 10.08SLRR83 pKa = 11.84SVTLSFVNAKK93 pKa = 9.92KK94 pKa = 10.08IRR96 pKa = 11.84RR97 pKa = 11.84LL98 pKa = 3.37

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

228

0

228

36831

34

2107

161.5

18.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.178 ± 0.381

1.005 ± 0.109

6.435 ± 0.188

6.88 ± 0.323

3.877 ± 0.125

7.855 ± 0.227

1.83 ± 0.132

5.387 ± 0.143

6.133 ± 0.252

7.035 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.87 ± 0.121

4.874 ± 0.143

3.769 ± 0.145

3.141 ± 0.152

5.512 ± 0.209

5.9 ± 0.226

6.09 ± 0.35

7.347 ± 0.205

1.884 ± 0.084

3.999 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski