Apis mellifera associated microvirus 50

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UU36|A0A3S8UU36_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 50 OX=2494781 PE=3 SV=1
MM1 pKa = 7.29EE2 pKa = 6.15LSMKK6 pKa = 10.37KK7 pKa = 10.14LSEE10 pKa = 4.12VVKK13 pKa = 10.54EE14 pKa = 4.07FFGKK18 pKa = 10.17SRR20 pKa = 11.84LDD22 pKa = 3.13EE23 pKa = 4.68RR24 pKa = 11.84YY25 pKa = 9.55PGGSPLEE32 pKa = 4.2VCNPQPVEE40 pKa = 3.97VPLRR44 pKa = 11.84FQRR47 pKa = 11.84PPTMEE52 pKa = 3.27EE53 pKa = 4.05RR54 pKa = 11.84IRR56 pKa = 11.84QFIRR60 pKa = 11.84SEE62 pKa = 3.73AMRR65 pKa = 11.84RR66 pKa = 11.84EE67 pKa = 4.15AEE69 pKa = 3.98AQGAEE74 pKa = 4.27TFEE77 pKa = 4.16EE78 pKa = 4.56ADD80 pKa = 4.06DD81 pKa = 5.08FEE83 pKa = 6.68VDD85 pKa = 5.39DD86 pKa = 5.35PDD88 pKa = 4.82FDD90 pKa = 4.23VPSSEE95 pKa = 4.28FEE97 pKa = 4.23VMEE100 pKa = 5.4LEE102 pKa = 4.48PQDD105 pKa = 4.41RR106 pKa = 11.84DD107 pKa = 3.3AAPPAEE113 pKa = 4.52PTSPQPPAAAPGPAQGAGPSGTPPAPAGGVPAAPGPAASS152 pKa = 3.94

Molecular weight: 16.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU72|A0A3S8UU72_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 50 OX=2494781 PE=4 SV=1
HHH2 pKa = 8.13IPHHH6 pKa = 4.78KKK8 pKa = 9.71RR9 pKa = 11.84YYY11 pKa = 7.96LKKK14 pKa = 10.17GVMEEE19 pKa = 5.03GCGRR23 pKa = 11.84CMPCRR28 pKa = 11.84INRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84EEE35 pKa = 3.6TSRR38 pKa = 11.84MMLEEE43 pKa = 4.3QGHHH47 pKa = 4.76VSWFVTLTYYY57 pKa = 10.65PEEE60 pKa = 3.98LPEEE64 pKa = 4.51GTLVPKKK71 pKa = 10.34HH72 pKa = 5.61QDDD75 pKa = 2.73LKKK78 pKa = 10.7LRR80 pKa = 11.84TATGEEE86 pKa = 4.03KK87 pKa = 9.64RR88 pKa = 11.84FYYY91 pKa = 11.25VGEEE95 pKa = 4.39YY96 pKa = 10.64DDD98 pKa = 4.02KK99 pKa = 10.01WRR101 pKa = 11.84PHHH104 pKa = 3.78YY105 pKa = 10.2HH106 pKa = 6.94ILFGGPSTPDDD117 pKa = 4.21ILATWHHH124 pKa = 6.53GLGHHH129 pKa = 5.98GFVTAEEE136 pKa = 4.06IQYYY140 pKa = 7.97ASYYY144 pKa = 10.33EEE146 pKa = 4.61KK147 pKa = 10.91KK148 pKa = 7.21TSSKKK153 pKa = 10.87DD154 pKa = 3.09DD155 pKa = 3.85LNGRR159 pKa = 11.84YYY161 pKa = 9.31EEE163 pKa = 4.18ARR165 pKa = 11.84MSRR168 pKa = 11.84KKK170 pKa = 9.59GIGALAVDDD179 pKa = 3.86MFGKKK184 pKa = 10.68LTSKKK189 pKa = 10.54GVDDD193 pKa = 3.6IIRR196 pKa = 11.84EEE198 pKa = 4.09DDD200 pKa = 3.61PSMVRR205 pKa = 11.84FEEE208 pKa = 4.28KKK210 pKa = 9.11KK211 pKa = 8.96PLGRR215 pKa = 11.84YYY217 pKa = 8.2RR218 pKa = 11.84SRR220 pKa = 11.84ARR222 pKa = 11.84AAAGFEEE229 pKa = 4.09EE230 pKa = 5.54GMPEEE235 pKa = 4.57DD236 pKa = 5.03FIQMALEEE244 pKa = 4.17QATLSDDD251 pKa = 3.35EEE253 pKa = 3.75FYYY256 pKa = 10.7HHH258 pKa = 5.78EE259 pKa = 4.47DDD261 pKa = 4.48VNQEEE266 pKa = 3.3TAKKK270 pKa = 10.25RR271 pKa = 11.84AKKK274 pKa = 9.92RR275 pKa = 11.84ASKKK279 pKa = 10.61SI

Molecular weight: 32.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1480

152

525

296.0

32.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.932 ± 1.664

0.811 ± 0.237

4.73 ± 0.382

6.014 ± 1.209

3.311 ± 0.528

8.108 ± 0.532

2.432 ± 0.404

3.581 ± 0.723

3.784 ± 0.58

7.365 ± 0.561

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.297

2.5 ± 0.529

7.365 ± 1.58

3.243 ± 0.582

9.459 ± 1.734

8.378 ± 0.886

4.459 ± 0.305

6.689 ± 0.33

1.824 ± 0.344

3.446 ± 0.734
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski