Amino acid dipepetide frequency for Mycobacterium phage Tonenili

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.77AlaAla: 10.77 ± 0.563
0.75AlaCys: 0.75 ± 0.11
7.018AlaAsp: 7.018 ± 0.449
6.693AlaGlu: 6.693 ± 0.532
2.799AlaPhe: 2.799 ± 0.191
7.464AlaGly: 7.464 ± 0.547
1.967AlaHis: 1.967 ± 0.21
4.117AlaIle: 4.117 ± 0.281
3.975AlaLys: 3.975 ± 0.272
8.092AlaLeu: 8.092 ± 0.49
2.677AlaMet: 2.677 ± 0.244
3.245AlaAsn: 3.245 ± 0.272
4.908AlaPro: 4.908 ± 0.464
4.279AlaGln: 4.279 ± 0.348
6.734AlaArg: 6.734 ± 0.466
5.476AlaSer: 5.476 ± 0.355
5.598AlaThr: 5.598 ± 0.453
6.957AlaVal: 6.957 ± 0.42
1.481AlaTrp: 1.481 ± 0.137
2.86AlaTyr: 2.86 ± 0.228
0.0AlaXaa: 0.0 ± 0.0
Cys
0.771CysAla: 0.771 ± 0.117
0.183CysCys: 0.183 ± 0.061
0.608CysAsp: 0.608 ± 0.102
0.548CysGlu: 0.548 ± 0.09
0.325CysPhe: 0.325 ± 0.093
1.197CysGly: 1.197 ± 0.159
0.264CysHis: 0.264 ± 0.081
0.406CysIle: 0.406 ± 0.084
0.183CysLys: 0.183 ± 0.057
0.629CysLeu: 0.629 ± 0.125
0.203CysMet: 0.203 ± 0.075
0.223CysAsn: 0.223 ± 0.062
0.771CysPro: 0.771 ± 0.149
0.385CysGln: 0.385 ± 0.084
0.507CysArg: 0.507 ± 0.1
0.406CysSer: 0.406 ± 0.09
0.406CysThr: 0.406 ± 0.11
0.466CysVal: 0.466 ± 0.088
0.223CysTrp: 0.223 ± 0.068
0.223CysTyr: 0.223 ± 0.074
0.0CysXaa: 0.0 ± 0.0
Asp
6.206AspAla: 6.206 ± 0.406
0.629AspCys: 0.629 ± 0.122
5.476AspAsp: 5.476 ± 0.463
5.557AspGlu: 5.557 ± 0.31
2.211AspPhe: 2.211 ± 0.219
5.172AspGly: 5.172 ± 0.337
1.907AspHis: 1.907 ± 0.185
2.738AspIle: 2.738 ± 0.232
2.616AspLys: 2.616 ± 0.274
5.821AspLeu: 5.821 ± 0.355
1.785AspMet: 1.785 ± 0.199
2.048AspAsn: 2.048 ± 0.202
5.192AspPro: 5.192 ± 0.37
2.353AspGln: 2.353 ± 0.228
4.847AspArg: 4.847 ± 0.393
3.59AspSer: 3.59 ± 0.283
3.712AspThr: 3.712 ± 0.311
4.665AspVal: 4.665 ± 0.296
1.379AspTrp: 1.379 ± 0.146
2.616AspTyr: 2.616 ± 0.213
0.0AspXaa: 0.0 ± 0.0
Glu
6.389GluAla: 6.389 ± 0.428
0.487GluCys: 0.487 ± 0.112
4.827GluAsp: 4.827 ± 0.395
4.665GluGlu: 4.665 ± 0.419
1.805GluPhe: 1.805 ± 0.195
4.077GluGly: 4.077 ± 0.308
2.069GluHis: 2.069 ± 0.255
3.407GluIle: 3.407 ± 0.282
2.292GluLys: 2.292 ± 0.211
5.476GluLeu: 5.476 ± 0.316
1.744GluMet: 1.744 ± 0.191
1.46GluAsn: 1.46 ± 0.156
2.9GluPro: 2.9 ± 0.245
2.819GluGln: 2.819 ± 0.245
4.766GluArg: 4.766 ± 0.312
3.549GluSer: 3.549 ± 0.266
3.245GluThr: 3.245 ± 0.251
4.969GluVal: 4.969 ± 0.329
1.562GluTrp: 1.562 ± 0.172
1.947GluTyr: 1.947 ± 0.231
0.0GluXaa: 0.0 ± 0.0
Phe
2.981PheAla: 2.981 ± 0.229
0.264PheCys: 0.264 ± 0.08
2.596PheAsp: 2.596 ± 0.186
1.988PheGlu: 1.988 ± 0.191
0.73PhePhe: 0.73 ± 0.114
2.616PheGly: 2.616 ± 0.232
0.669PheHis: 0.669 ± 0.126
1.278PheIle: 1.278 ± 0.138
1.055PheLys: 1.055 ± 0.138
2.231PheLeu: 2.231 ± 0.209
0.548PheMet: 0.548 ± 0.125
1.095PheAsn: 1.095 ± 0.133
1.501PhePro: 1.501 ± 0.153
1.278PheGln: 1.278 ± 0.158
2.272PheArg: 2.272 ± 0.187
1.46PheSer: 1.46 ± 0.173
2.069PheThr: 2.069 ± 0.21
2.332PheVal: 2.332 ± 0.209
0.568PheTrp: 0.568 ± 0.123
1.237PheTyr: 1.237 ± 0.167
0.0PheXaa: 0.0 ± 0.0
Gly
6.49GlyAla: 6.49 ± 0.471
0.629GlyCys: 0.629 ± 0.125
4.969GlyAsp: 4.969 ± 0.338
4.462GlyGlu: 4.462 ± 0.323
2.434GlyPhe: 2.434 ± 0.239
7.829GlyGly: 7.829 ± 1.026
1.825GlyHis: 1.825 ± 0.251
3.651GlyIle: 3.651 ± 0.32
3.407GlyLys: 3.407 ± 0.304
6.227GlyLeu: 6.227 ± 0.346
2.19GlyMet: 2.19 ± 0.235
2.637GlyAsn: 2.637 ± 0.286
4.077GlyPro: 4.077 ± 0.412
3.326GlyGln: 3.326 ± 0.261
4.868GlyArg: 4.868 ± 0.324
4.523GlySer: 4.523 ± 0.393
5.294GlyThr: 5.294 ± 0.34
5.273GlyVal: 5.273 ± 0.314
1.886GlyTrp: 1.886 ± 0.197
2.495GlyTyr: 2.495 ± 0.249
0.0GlyXaa: 0.0 ± 0.0
His
2.231HisAla: 2.231 ± 0.216
0.183HisCys: 0.183 ± 0.055
1.663HisAsp: 1.663 ± 0.194
1.521HisGlu: 1.521 ± 0.205
0.852HisPhe: 0.852 ± 0.125
1.785HisGly: 1.785 ± 0.207
0.953HisHis: 0.953 ± 0.154
0.872HisIle: 0.872 ± 0.115
0.71HisLys: 0.71 ± 0.112
2.15HisLeu: 2.15 ± 0.294
0.406HisMet: 0.406 ± 0.088
0.608HisAsn: 0.608 ± 0.119
2.008HisPro: 2.008 ± 0.237
1.055HisGln: 1.055 ± 0.133
1.927HisArg: 1.927 ± 0.24
1.197HisSer: 1.197 ± 0.171
1.379HisThr: 1.379 ± 0.177
2.028HisVal: 2.028 ± 0.215
0.385HisTrp: 0.385 ± 0.076
0.75HisTyr: 0.75 ± 0.119
0.0HisXaa: 0.0 ± 0.0
Ile
4.847IleAla: 4.847 ± 0.313
0.406IleCys: 0.406 ± 0.083
3.468IleAsp: 3.468 ± 0.272
3.123IleGlu: 3.123 ± 0.225
1.116IlePhe: 1.116 ± 0.149
3.265IleGly: 3.265 ± 0.289
1.014IleHis: 1.014 ± 0.12
1.663IleIle: 1.663 ± 0.19
1.562IleLys: 1.562 ± 0.174
2.657IleLeu: 2.657 ± 0.248
0.913IleMet: 0.913 ± 0.129
1.785IleAsn: 1.785 ± 0.184
2.556IlePro: 2.556 ± 0.231
1.663IleGln: 1.663 ± 0.182
3.225IleArg: 3.225 ± 0.308
2.332IleSer: 2.332 ± 0.255
2.921IleThr: 2.921 ± 0.229
3.164IleVal: 3.164 ± 0.271
0.71IleTrp: 0.71 ± 0.102
1.257IleTyr: 1.257 ± 0.17
0.0IleXaa: 0.0 ± 0.0
Lys
4.279LysAla: 4.279 ± 0.321
0.325LysCys: 0.325 ± 0.095
2.089LysAsp: 2.089 ± 0.199
2.13LysGlu: 2.13 ± 0.218
1.116LysPhe: 1.116 ± 0.134
2.596LysGly: 2.596 ± 0.202
0.771LysHis: 0.771 ± 0.134
1.825LysIle: 1.825 ± 0.201
1.541LysLys: 1.541 ± 0.223
3.286LysLeu: 3.286 ± 0.23
1.034LysMet: 1.034 ± 0.159
1.339LysAsn: 1.339 ± 0.183
2.069LysPro: 2.069 ± 0.193
1.197LysGln: 1.197 ± 0.158
2.758LysArg: 2.758 ± 0.243
1.907LysSer: 1.907 ± 0.218
2.576LysThr: 2.576 ± 0.201
2.961LysVal: 2.961 ± 0.271
0.669LysTrp: 0.669 ± 0.129
1.176LysTyr: 1.176 ± 0.173
0.0LysXaa: 0.0 ± 0.0
Leu
8.133LeuAla: 8.133 ± 0.38
0.832LeuCys: 0.832 ± 0.146
6.105LeuAsp: 6.105 ± 0.42
4.178LeuGlu: 4.178 ± 0.313
2.008LeuPhe: 2.008 ± 0.185
5.294LeuGly: 5.294 ± 0.278
1.481LeuHis: 1.481 ± 0.16
3.509LeuIle: 3.509 ± 0.325
3.083LeuLys: 3.083 ± 0.283
5.679LeuLeu: 5.679 ± 0.404
2.231LeuMet: 2.231 ± 0.264
3.144LeuAsn: 3.144 ± 0.28
4.259LeuPro: 4.259 ± 0.295
2.819LeuGln: 2.819 ± 0.233
5.456LeuArg: 5.456 ± 0.371
4.401LeuSer: 4.401 ± 0.333
4.665LeuThr: 4.665 ± 0.406
5.578LeuVal: 5.578 ± 0.327
1.197LeuTrp: 1.197 ± 0.138
2.332LeuTyr: 2.332 ± 0.242
0.0LeuXaa: 0.0 ± 0.0
Met
3.022MetAla: 3.022 ± 0.282
0.101MetCys: 0.101 ± 0.05
1.623MetAsp: 1.623 ± 0.185
1.298MetGlu: 1.298 ± 0.139
0.832MetPhe: 0.832 ± 0.144
2.089MetGly: 2.089 ± 0.254
0.507MetHis: 0.507 ± 0.128
0.892MetIle: 0.892 ± 0.12
1.095MetLys: 1.095 ± 0.143
1.521MetLeu: 1.521 ± 0.165
0.75MetMet: 0.75 ± 0.141
0.832MetAsn: 0.832 ± 0.128
1.501MetPro: 1.501 ± 0.165
0.892MetGln: 0.892 ± 0.134
1.623MetArg: 1.623 ± 0.154
2.312MetSer: 2.312 ± 0.217
2.353MetThr: 2.353 ± 0.215
1.278MetVal: 1.278 ± 0.151
0.385MetTrp: 0.385 ± 0.084
0.487MetTyr: 0.487 ± 0.104
0.0MetXaa: 0.0 ± 0.0
Asn
2.819AsnAla: 2.819 ± 0.206
0.243AsnCys: 0.243 ± 0.072
1.988AsnAsp: 1.988 ± 0.205
1.825AsnGlu: 1.825 ± 0.193
1.318AsnPhe: 1.318 ± 0.186
3.083AsnGly: 3.083 ± 0.249
0.791AsnHis: 0.791 ± 0.128
1.278AsnIle: 1.278 ± 0.154
1.541AsnLys: 1.541 ± 0.182
2.109AsnLeu: 2.109 ± 0.226
0.974AsnMet: 0.974 ± 0.141
1.197AsnAsn: 1.197 ± 0.188
2.434AsnPro: 2.434 ± 0.2
1.399AsnGln: 1.399 ± 0.184
2.556AsnArg: 2.556 ± 0.213
1.744AsnSer: 1.744 ± 0.181
2.048AsnThr: 2.048 ± 0.22
1.805AsnVal: 1.805 ± 0.187
0.73AsnTrp: 0.73 ± 0.112
1.055AsnTyr: 1.055 ± 0.157
0.0AsnXaa: 0.0 ± 0.0
Pro
4.949ProAla: 4.949 ± 0.368
0.466ProCys: 0.466 ± 0.104
5.172ProAsp: 5.172 ± 0.339
4.361ProGlu: 4.361 ± 0.399
1.623ProPhe: 1.623 ± 0.18
5.436ProGly: 5.436 ± 0.453
1.44ProHis: 1.44 ± 0.199
2.13ProIle: 2.13 ± 0.233
1.886ProLys: 1.886 ± 0.217
3.265ProLeu: 3.265 ± 0.265
1.359ProMet: 1.359 ± 0.165
2.251ProAsn: 2.251 ± 0.183
3.265ProPro: 3.265 ± 0.496
2.048ProGln: 2.048 ± 0.2
3.123ProArg: 3.123 ± 0.243
3.042ProSer: 3.042 ± 0.254
3.752ProThr: 3.752 ± 0.297
4.421ProVal: 4.421 ± 0.235
1.176ProTrp: 1.176 ± 0.178
1.602ProTyr: 1.602 ± 0.215
0.0ProXaa: 0.0 ± 0.0
Gln
4.523GlnAla: 4.523 ± 0.411
0.264GlnCys: 0.264 ± 0.074
2.17GlnAsp: 2.17 ± 0.218
2.19GlnGlu: 2.19 ± 0.255
1.683GlnPhe: 1.683 ± 0.181
2.515GlnGly: 2.515 ± 0.257
1.156GlnHis: 1.156 ± 0.184
2.069GlnIle: 2.069 ± 0.163
1.055GlnLys: 1.055 ± 0.159
3.732GlnLeu: 3.732 ± 0.313
1.339GlnMet: 1.339 ± 0.144
1.156GlnAsn: 1.156 ± 0.151
1.785GlnPro: 1.785 ± 0.177
1.744GlnGln: 1.744 ± 0.217
3.306GlnArg: 3.306 ± 0.296
2.19GlnSer: 2.19 ± 0.199
2.17GlnThr: 2.17 ± 0.186
2.961GlnVal: 2.961 ± 0.286
0.832GlnTrp: 0.832 ± 0.129
1.257GlnTyr: 1.257 ± 0.167
0.0GlnXaa: 0.0 ± 0.0
Arg
6.49ArgAla: 6.49 ± 0.404
0.669ArgCys: 0.669 ± 0.113
4.3ArgAsp: 4.3 ± 0.307
5.395ArgGlu: 5.395 ± 0.287
2.13ArgPhe: 2.13 ± 0.232
4.158ArgGly: 4.158 ± 0.342
1.947ArgHis: 1.947 ± 0.238
3.184ArgIle: 3.184 ± 0.253
3.651ArgLys: 3.651 ± 0.277
4.949ArgLeu: 4.949 ± 0.337
1.825ArgMet: 1.825 ± 0.223
2.272ArgAsn: 2.272 ± 0.191
3.306ArgPro: 3.306 ± 0.273
3.123ArgGln: 3.123 ± 0.243
5.395ArgArg: 5.395 ± 0.402
3.549ArgSer: 3.549 ± 0.23
3.894ArgThr: 3.894 ± 0.288
5.253ArgVal: 5.253 ± 0.387
1.298ArgTrp: 1.298 ± 0.163
2.373ArgTyr: 2.373 ± 0.228
0.0ArgXaa: 0.0 ± 0.0
Ser
4.969SerAla: 4.969 ± 0.401
0.507SerCys: 0.507 ± 0.098
3.671SerAsp: 3.671 ± 0.376
2.88SerGlu: 2.88 ± 0.24
1.765SerPhe: 1.765 ± 0.204
5.679SerGly: 5.679 ± 0.454
0.913SerHis: 0.913 ± 0.144
2.535SerIle: 2.535 ± 0.269
1.907SerLys: 1.907 ± 0.22
4.34SerLeu: 4.34 ± 0.281
1.339SerMet: 1.339 ± 0.171
1.541SerAsn: 1.541 ± 0.162
2.921SerPro: 2.921 ± 0.233
2.13SerGln: 2.13 ± 0.182
3.488SerArg: 3.488 ± 0.282
3.651SerSer: 3.651 ± 0.357
4.138SerThr: 4.138 ± 0.301
3.996SerVal: 3.996 ± 0.32
1.116SerTrp: 1.116 ± 0.156
1.744SerTyr: 1.744 ± 0.184
0.0SerXaa: 0.0 ± 0.0
Thr
6.145ThrAla: 6.145 ± 0.426
0.588ThrCys: 0.588 ± 0.116
4.117ThrAsp: 4.117 ± 0.358
3.63ThrGlu: 3.63 ± 0.297
2.292ThrPhe: 2.292 ± 0.26
5.395ThrGly: 5.395 ± 0.394
1.683ThrHis: 1.683 ± 0.19
3.042ThrIle: 3.042 ± 0.252
2.332ThrLys: 2.332 ± 0.208
4.787ThrLeu: 4.787 ± 0.32
1.359ThrMet: 1.359 ± 0.18
2.272ThrAsn: 2.272 ± 0.283
4.523ThrPro: 4.523 ± 0.366
2.332ThrGln: 2.332 ± 0.237
3.387ThrArg: 3.387 ± 0.265
3.549ThrSer: 3.549 ± 0.281
4.807ThrThr: 4.807 ± 0.426
4.726ThrVal: 4.726 ± 0.36
1.318ThrTrp: 1.318 ± 0.153
1.785ThrTyr: 1.785 ± 0.177
0.0ThrXaa: 0.0 ± 0.0
Val
7.139ValAla: 7.139 ± 0.413
0.791ValCys: 0.791 ± 0.144
5.03ValAsp: 5.03 ± 0.258
5.152ValGlu: 5.152 ± 0.347
2.231ValPhe: 2.231 ± 0.241
5.273ValGly: 5.273 ± 0.348
1.805ValHis: 1.805 ± 0.176
3.347ValIle: 3.347 ± 0.269
2.434ValLys: 2.434 ± 0.274
5.395ValLeu: 5.395 ± 0.288
1.582ValMet: 1.582 ± 0.147
2.292ValAsn: 2.292 ± 0.275
4.381ValPro: 4.381 ± 0.273
2.839ValGln: 2.839 ± 0.31
4.888ValArg: 4.888 ± 0.348
3.793ValSer: 3.793 ± 0.255
5.273ValThr: 5.273 ± 0.343
6.247ValVal: 6.247 ± 0.471
1.055ValTrp: 1.055 ± 0.128
2.089ValTyr: 2.089 ± 0.225
0.0ValXaa: 0.0 ± 0.0
Trp
1.582TrpAla: 1.582 ± 0.182
0.264TrpCys: 0.264 ± 0.074
1.339TrpAsp: 1.339 ± 0.158
1.156TrpGlu: 1.156 ± 0.178
0.588TrpPhe: 0.588 ± 0.118
1.399TrpGly: 1.399 ± 0.19
0.487TrpHis: 0.487 ± 0.085
0.73TrpIle: 0.73 ± 0.124
0.527TrpLys: 0.527 ± 0.105
1.197TrpLeu: 1.197 ± 0.162
0.466TrpMet: 0.466 ± 0.092
0.527TrpAsn: 0.527 ± 0.101
0.872TrpPro: 0.872 ± 0.134
0.933TrpGln: 0.933 ± 0.127
1.704TrpArg: 1.704 ± 0.171
1.095TrpSer: 1.095 ± 0.151
1.704TrpThr: 1.704 ± 0.173
1.359TrpVal: 1.359 ± 0.187
0.588TrpTrp: 0.588 ± 0.125
0.588TrpTyr: 0.588 ± 0.085
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.123TyrAla: 3.123 ± 0.263
0.345TyrCys: 0.345 ± 0.09
2.292TyrAsp: 2.292 ± 0.227
1.704TyrGlu: 1.704 ± 0.194
0.913TyrPhe: 0.913 ± 0.151
2.17TyrGly: 2.17 ± 0.26
1.014TyrHis: 1.014 ± 0.171
0.974TyrIle: 0.974 ± 0.128
0.791TyrLys: 0.791 ± 0.123
2.839TyrLeu: 2.839 ± 0.223
0.649TyrMet: 0.649 ± 0.113
1.014TyrAsn: 1.014 ± 0.144
1.562TyrPro: 1.562 ± 0.191
1.481TyrGln: 1.481 ± 0.183
2.332TyrArg: 2.332 ± 0.242
1.46TyrSer: 1.46 ± 0.161
2.069TyrThr: 2.069 ± 0.228
2.596TyrVal: 2.596 ± 0.266
0.548TyrTrp: 0.548 ± 0.102
0.974TyrTyr: 0.974 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 258 proteins (49306 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski