Diploscapter pachys

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Rhabditidae incertae sedis; Diploscapter

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2KTF8|A0A2A2KTF8_9BILA Uncharacterized protein OS=Diploscapter pachys OX=2018661 GN=WR25_17893 PE=3 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84GGAGADD8 pKa = 3.56RR9 pKa = 11.84FIGGAGNDD17 pKa = 3.64TVSYY21 pKa = 9.22TDD23 pKa = 3.38AGAGITLNFKK33 pKa = 9.82TGVHH37 pKa = 5.77TGIAAGDD44 pKa = 3.73TFDD47 pKa = 5.87SIEE50 pKa = 4.24LFQGSNYY57 pKa = 10.35ADD59 pKa = 3.5TFISDD64 pKa = 3.31ARR66 pKa = 11.84AHH68 pKa = 5.34TFEE71 pKa = 5.95GGLNDD76 pKa = 3.47TMDD79 pKa = 3.66YY80 pKa = 10.8SLSEE84 pKa = 3.9QAVTITIDD92 pKa = 3.26NTGGKK97 pKa = 9.07GQGGDD102 pKa = 3.41AEE104 pKa = 4.54GDD106 pKa = 3.67KK107 pKa = 10.15FTGMYY112 pKa = 10.09SVIGSSQNDD121 pKa = 3.9TFIMQATGSSKK132 pKa = 10.81SFAGGAGDD140 pKa = 4.38DD141 pKa = 3.67VYY143 pKa = 11.62YY144 pKa = 10.78LHH146 pKa = 6.44NTAGVFEE153 pKa = 4.22QAGGGIDD160 pKa = 3.59EE161 pKa = 4.62VRR163 pKa = 11.84TTLASYY169 pKa = 9.36TLTPEE174 pKa = 4.39TGVHH178 pKa = 5.44TGIAAGDD185 pKa = 3.72TFDD188 pKa = 5.35SIEE191 pKa = 4.01VFQGSNYY198 pKa = 10.33ADD200 pKa = 3.5TFISDD205 pKa = 3.31ARR207 pKa = 11.84AHH209 pKa = 5.34TFEE212 pKa = 5.97GGLNDD217 pKa = 3.68TLDD220 pKa = 3.74YY221 pKa = 11.02SLSEE225 pKa = 3.94QAVKK229 pKa = 9.06ITIEE233 pKa = 4.2GNGGSGQGGDD243 pKa = 3.47AEE245 pKa = 4.53GDD247 pKa = 3.67KK248 pKa = 10.15FTGMYY253 pKa = 10.09SVIGSSQNDD262 pKa = 3.9TFIMQATGSSKK273 pKa = 10.71NFAGGAGDD281 pKa = 3.88DD282 pKa = 3.69VYY284 pKa = 11.74YY285 pKa = 10.59LYY287 pKa = 10.05STAGVFEE294 pKa = 4.15QAGGGIDD301 pKa = 3.59EE302 pKa = 4.89VRR304 pKa = 11.84TTLANYY310 pKa = 7.48TLTNEE315 pKa = 4.23VEE317 pKa = 4.09NLTFIGTGNFTGYY330 pKa = 11.14GNASNNVIVGGAGNDD345 pKa = 3.73TLFGGAGADD354 pKa = 3.4RR355 pKa = 11.84FVGGAGIDD363 pKa = 3.58TVSYY367 pKa = 10.53GDD369 pKa = 3.64SSSGILLNLKK379 pKa = 8.67TGQHH383 pKa = 5.57GGIAEE388 pKa = 4.42GDD390 pKa = 3.6TFEE393 pKa = 6.25GIEE396 pKa = 4.18KK397 pKa = 10.4FVGSSLGDD405 pKa = 3.09VFVANDD411 pKa = 3.24QANDD415 pKa = 3.38FNGAGGVDD423 pKa = 4.13LLTFAGEE430 pKa = 4.43GAGITLDD437 pKa = 4.7LNATGNDD444 pKa = 3.45LFTSIEE450 pKa = 4.29SFEE453 pKa = 3.97GTAFNDD459 pKa = 3.89TFIGTAASEE468 pKa = 4.13NFIGGAGADD477 pKa = 4.15VINGGAGRR485 pKa = 11.84DD486 pKa = 3.8SAWYY490 pKa = 8.7ISSNASVSVDD500 pKa = 3.3LATGVVGGGHH510 pKa = 7.01AEE512 pKa = 3.88GDD514 pKa = 3.82VLSGIEE520 pKa = 4.24GVAGSAFADD529 pKa = 3.95VLNGDD534 pKa = 3.82AGANTLYY541 pKa = 10.67GAEE544 pKa = 4.38GDD546 pKa = 4.08DD547 pKa = 4.66VINGGAGNDD556 pKa = 3.22ILYY559 pKa = 10.9GDD561 pKa = 5.43GIYY564 pKa = 9.49STDD567 pKa = 3.47FGALARR573 pKa = 11.84PANTQVAQADD583 pKa = 4.49TINGGDD589 pKa = 3.95GDD591 pKa = 4.16DD592 pKa = 4.65TITGALNDD600 pKa = 4.0TGSIYY605 pKa = 10.83HH606 pKa = 7.02GDD608 pKa = 3.66AGNDD612 pKa = 3.73TIQTANGKK620 pKa = 10.3AYY622 pKa = 10.39GDD624 pKa = 4.37DD625 pKa = 3.93GNDD628 pKa = 2.89RR629 pKa = 11.84LVGNGYY635 pKa = 10.65AYY637 pKa = 10.13EE638 pKa = 4.32LYY640 pKa = 10.67GGAGADD646 pKa = 3.66RR647 pKa = 11.84LDD649 pKa = 3.77MMSAGYY655 pKa = 10.85AFGGEE660 pKa = 4.1GSDD663 pKa = 3.88IYY665 pKa = 10.77TIYY668 pKa = 10.91SKK670 pKa = 11.13AGVVIQDD677 pKa = 3.69TGTAGIDD684 pKa = 3.41VVVLKK689 pKa = 10.82NIQTFQDD696 pKa = 3.36VVVQNDD702 pKa = 3.73GVNAYY707 pKa = 9.3IFSKK711 pKa = 10.97ADD713 pKa = 3.2WNAGNLDD720 pKa = 3.96SGVMLSNWYY729 pKa = 10.08AGSNTIEE736 pKa = 4.08SFQTNNGDD744 pKa = 3.52TFTITT749 pKa = 3.44

Molecular weight: 76.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2KBM4|A0A2A2KBM4_9BILA Uncharacterized protein OS=Diploscapter pachys OX=2018661 GN=WR25_03065 PE=4 SV=1
MM1 pKa = 7.69AAPAAAVVSWPRR13 pKa = 11.84PRR15 pKa = 11.84RR16 pKa = 11.84PRR18 pKa = 11.84RR19 pKa = 11.84ARR21 pKa = 11.84GSIALASAMRR31 pKa = 11.84AIARR35 pKa = 11.84APLAARR41 pKa = 11.84SASRR45 pKa = 11.84VPASAAASPATAATPGVASTSPPASTVPAPGSNSPMAPSRR85 pKa = 11.84TPRR88 pKa = 11.84TAALPRR94 pKa = 11.84PPASTAPAAA103 pKa = 3.99

Molecular weight: 10.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27206

6775

33981

15129975

49

10228

445.2

49.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.323 ± 0.017

1.858 ± 0.011

5.576 ± 0.012

6.73 ± 0.019

3.993 ± 0.011

5.852 ± 0.013

2.375 ± 0.007

5.664 ± 0.011

5.91 ± 0.021

8.656 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.007

4.554 ± 0.011

5.19 ± 0.016

4.494 ± 0.013

5.968 ± 0.014

7.839 ± 0.019

5.484 ± 0.013

5.977 ± 0.012

1.086 ± 0.005

2.925 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski