Amino acid dipepetide frequency for Eurypyga helias (Sunbittern)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.682AlaAla: 5.682 ± 0.061	1.327AlaCys: 1.327 ± 0.026	3.06AlaAsp: 3.06 ± 0.031	4.622AlaGlu: 4.622 ± 0.05	2.653AlaPhe: 2.653 ± 0.034	3.866AlaGly: 3.866 ± 0.041	1.354AlaHis: 1.354 ± 0.023	3.093AlaIle: 3.093 ± 0.034	3.689AlaLys: 3.689 ± 0.036	6.482AlaLeu: 6.482 ± 0.056	1.5AlaMet: 1.5 ± 0.022	2.23AlaAsn: 2.23 ± 0.026	2.951AlaPro: 2.951 ± 0.038	2.741AlaGln: 2.741 ± 0.032	3.043AlaArg: 3.043 ± 0.038	5.219AlaSer: 5.219 ± 0.044	3.309AlaThr: 3.309 ± 0.037	5.036AlaVal: 5.036 ± 0.044	0.704AlaTrp: 0.704 ± 0.016	1.68AlaTyr: 1.68 ± 0.024	0.002AlaXaa: 0.002 ± 0.001
Cys	1.182CysAla: 1.182 ± 0.02	0.628CysCys: 0.628 ± 0.016	1.063CysAsp: 1.063 ± 0.029	1.273CysGlu: 1.273 ± 0.026	0.95CysPhe: 0.95 ± 0.019	1.455CysGly: 1.455 ± 0.026	0.637CysHis: 0.637 ± 0.016	1.119CysIle: 1.119 ± 0.023	1.343CysLys: 1.343 ± 0.025	2.168CysLeu: 2.168 ± 0.031	0.436CysMet: 0.436 ± 0.012	0.922CysAsn: 0.922 ± 0.021	1.197CysPro: 1.197 ± 0.027	1.049CysGln: 1.049 ± 0.024	1.22CysArg: 1.22 ± 0.023	1.998CysSer: 1.998 ± 0.027	1.193CysThr: 1.193 ± 0.023	1.342CysVal: 1.342 ± 0.022	0.296CysTrp: 0.296 ± 0.01	0.697CysTyr: 0.697 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.017AspAla: 3.017 ± 0.03	1.111AspCys: 1.111 ± 0.024	2.864AspAsp: 2.864 ± 0.04	3.746AspGlu: 3.746 ± 0.042	2.317AspPhe: 2.317 ± 0.022	3.284AspGly: 3.284 ± 0.038	1.196AspHis: 1.196 ± 0.023	3.022AspIle: 3.022 ± 0.038	2.825AspLys: 2.825 ± 0.035	4.996AspLeu: 4.996 ± 0.044	1.154AspMet: 1.154 ± 0.018	1.961AspAsn: 1.961 ± 0.03	2.662AspPro: 2.662 ± 0.032	1.841AspGln: 1.841 ± 0.025	2.388AspArg: 2.388 ± 0.036	4.108AspSer: 4.108 ± 0.045	2.551AspThr: 2.551 ± 0.029	3.347AspVal: 3.347 ± 0.033	0.673AspTrp: 0.673 ± 0.015	1.679AspTyr: 1.679 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	4.796GluAla: 4.796 ± 0.051	1.287GluCys: 1.287 ± 0.031	4.509GluAsp: 4.509 ± 0.042	7.817GluGlu: 7.817 ± 0.1	2.222GluPhe: 2.222 ± 0.03	3.917GluGly: 3.917 ± 0.042	1.565GluHis: 1.565 ± 0.021	3.528GluIle: 3.528 ± 0.042	5.791GluLys: 5.791 ± 0.073	6.278GluLeu: 6.278 ± 0.059	1.762GluMet: 1.762 ± 0.026	3.457GluAsn: 3.457 ± 0.04	2.518GluPro: 2.518 ± 0.031	3.157GluGln: 3.157 ± 0.041	3.992GluArg: 3.992 ± 0.055	4.473GluSer: 4.473 ± 0.041	3.586GluThr: 3.586 ± 0.04	4.359GluVal: 4.359 ± 0.04	0.753GluTrp: 0.753 ± 0.017	1.878GluTyr: 1.878 ± 0.024	0.001GluXaa: 0.001 ± 0.0
Phe	2.158PheAla: 2.158 ± 0.028	1.01PheCys: 1.01 ± 0.017	1.834PheAsp: 1.834 ± 0.03	2.153PheGlu: 2.153 ± 0.028	1.951PhePhe: 1.951 ± 0.034	2.295PheGly: 2.295 ± 0.03	1.092PheHis: 1.092 ± 0.015	2.109PheIle: 2.109 ± 0.03	2.215PheLys: 2.215 ± 0.031	4.311PheLeu: 4.311 ± 0.051	0.833PheMet: 0.833 ± 0.016	1.536PheAsn: 1.536 ± 0.025	2.018PhePro: 2.018 ± 0.029	1.849PheGln: 1.849 ± 0.025	2.031PheArg: 2.031 ± 0.03	3.471PheSer: 3.471 ± 0.034	2.314PheThr: 2.314 ± 0.032	2.485PheVal: 2.485 ± 0.03	0.543PheTrp: 0.543 ± 0.014	1.329PheTyr: 1.329 ± 0.022	0.001PheXaa: 0.001 ± 0.0
Gly	3.369GlyAla: 3.369 ± 0.041	1.232GlyCys: 1.232 ± 0.023	2.927GlyAsp: 2.927 ± 0.035	3.703GlyGlu: 3.703 ± 0.052	2.555GlyPhe: 2.555 ± 0.033	3.719GlyGly: 3.719 ± 0.045	1.501GlyHis: 1.501 ± 0.024	3.131GlyIle: 3.131 ± 0.04	3.975GlyLys: 3.975 ± 0.044	5.159GlyLeu: 5.159 ± 0.057	1.4GlyMet: 1.4 ± 0.026	2.617GlyAsn: 2.617 ± 0.032	2.622GlyPro: 2.622 ± 0.072	2.388GlyGln: 2.388 ± 0.034	3.203GlyArg: 3.203 ± 0.04	4.996GlySer: 4.996 ± 0.05	3.393GlyThr: 3.393 ± 0.033	3.483GlyVal: 3.483 ± 0.036	0.77GlyTrp: 0.77 ± 0.019	1.945GlyTyr: 1.945 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	1.356HisAla: 1.356 ± 0.021	0.709HisCys: 0.709 ± 0.017	0.941HisAsp: 0.941 ± 0.017	1.359HisGlu: 1.359 ± 0.021	1.1HisPhe: 1.1 ± 0.017	1.486HisGly: 1.486 ± 0.025	0.869HisHis: 0.869 ± 0.019	1.348HisIle: 1.348 ± 0.021	1.399HisLys: 1.399 ± 0.023	2.8HisLeu: 2.8 ± 0.036	0.589HisMet: 0.589 ± 0.016	0.974HisAsn: 0.974 ± 0.017	1.496HisPro: 1.496 ± 0.023	1.169HisGln: 1.169 ± 0.022	1.461HisArg: 1.461 ± 0.024	2.197HisSer: 2.197 ± 0.03	1.333HisThr: 1.333 ± 0.026	1.57HisVal: 1.57 ± 0.024	0.435HisTrp: 0.435 ± 0.012	0.886HisTyr: 0.886 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	3.006IleAla: 3.006 ± 0.032	1.219IleCys: 1.219 ± 0.022	2.326IleAsp: 2.326 ± 0.024	2.869IleGlu: 2.869 ± 0.033	2.214IlePhe: 2.214 ± 0.035	2.519IleGly: 2.519 ± 0.032	1.438IleHis: 1.438 ± 0.023	2.777IleIle: 2.777 ± 0.036	3.038IleLys: 3.038 ± 0.035	4.944IleLeu: 4.944 ± 0.044	1.079IleMet: 1.079 ± 0.019	2.149IleAsn: 2.149 ± 0.032	2.901IlePro: 2.901 ± 0.035	2.409IleGln: 2.409 ± 0.033	2.617IleArg: 2.617 ± 0.03	4.102IleSer: 4.102 ± 0.038	2.9IleThr: 2.9 ± 0.034	2.957IleVal: 2.957 ± 0.038	0.597IleTrp: 0.597 ± 0.016	1.608IleTyr: 1.608 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	4.23LysAla: 4.23 ± 0.046	1.246LysCys: 1.246 ± 0.022	3.429LysAsp: 3.429 ± 0.041	5.68LysGlu: 5.68 ± 0.073	2.056LysPhe: 2.056 ± 0.028	3.373LysGly: 3.373 ± 0.05	1.63LysHis: 1.63 ± 0.025	3.245LysIle: 3.245 ± 0.036	5.504LysLys: 5.504 ± 0.072	5.852LysLeu: 5.852 ± 0.056	1.559LysMet: 1.559 ± 0.02	2.778LysAsn: 2.778 ± 0.035	3.121LysPro: 3.121 ± 0.039	2.961LysGln: 2.961 ± 0.041	3.611LysArg: 3.611 ± 0.037	4.305LysSer: 4.305 ± 0.049	3.431LysThr: 3.431 ± 0.035	3.76LysVal: 3.76 ± 0.034	0.687LysTrp: 0.687 ± 0.013	1.865LysTyr: 1.865 ± 0.025	0.001LysXaa: 0.001 ± 0.0
Leu	6.246LeuAla: 6.246 ± 0.057	2.17LeuCys: 2.17 ± 0.028	5.021LeuAsp: 5.021 ± 0.05	7.012LeuGlu: 7.012 ± 0.077	3.601LeuPhe: 3.601 ± 0.045	5.124LeuGly: 5.124 ± 0.046	2.684LeuHis: 2.684 ± 0.033	4.262LeuIle: 4.262 ± 0.042	6.503LeuLys: 6.503 ± 0.059	10.069LeuLeu: 10.069 ± 0.094	2.064LeuMet: 2.064 ± 0.032	3.865LeuAsn: 3.865 ± 0.041	5.473LeuPro: 5.473 ± 0.056	5.586LeuGln: 5.586 ± 0.061	5.186LeuArg: 5.186 ± 0.047	7.904LeuSer: 7.904 ± 0.064	5.0LeuThr: 5.0 ± 0.039	5.642LeuVal: 5.642 ± 0.053	1.074LeuTrp: 1.074 ± 0.022	2.846LeuTyr: 2.846 ± 0.032	0.001LeuXaa: 0.001 ± 0.001
Met	1.628MetAla: 1.628 ± 0.024	0.448MetCys: 0.448 ± 0.013	1.298MetAsp: 1.298 ± 0.019	1.928MetGlu: 1.928 ± 0.028	0.869MetPhe: 0.869 ± 0.018	1.248MetGly: 1.248 ± 0.021	0.502MetHis: 0.502 ± 0.014	0.983MetIle: 0.983 ± 0.019	1.631MetLys: 1.631 ± 0.023	2.095MetLeu: 2.095 ± 0.029	0.612MetMet: 0.612 ± 0.015	0.995MetAsn: 0.995 ± 0.019	1.027MetPro: 1.027 ± 0.024	1.021MetGln: 1.021 ± 0.022	1.052MetArg: 1.052 ± 0.02	1.536MetSer: 1.536 ± 0.022	1.156MetThr: 1.156 ± 0.022	1.461MetVal: 1.461 ± 0.021	0.257MetTrp: 0.257 ± 0.01	0.673MetTyr: 0.673 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.33AsnAla: 2.33 ± 0.028	0.969AsnCys: 0.969 ± 0.023	1.71AsnAsp: 1.71 ± 0.03	2.544AsnGlu: 2.544 ± 0.035	1.642AsnPhe: 1.642 ± 0.021	2.841AsnGly: 2.841 ± 0.04	1.003AsnHis: 1.003 ± 0.018	2.475AsnIle: 2.475 ± 0.033	2.571AsnLys: 2.571 ± 0.033	4.172AsnLeu: 4.172 ± 0.038	1.009AsnMet: 1.009 ± 0.02	1.836AsnAsn: 1.836 ± 0.028	2.259AsnPro: 2.259 ± 0.034	1.728AsnGln: 1.728 ± 0.024	2.137AsnArg: 2.137 ± 0.024	3.459AsnSer: 3.459 ± 0.038	2.231AsnThr: 2.231 ± 0.025	2.493AsnVal: 2.493 ± 0.031	0.503AsnTrp: 0.503 ± 0.012	1.335AsnTyr: 1.335 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	3.75ProAla: 3.75 ± 0.047	1.033ProCys: 1.033 ± 0.018	2.658ProAsp: 2.658 ± 0.03	3.874ProGlu: 3.874 ± 0.045	1.938ProPhe: 1.938 ± 0.029	3.579ProGly: 3.579 ± 0.112	1.244ProHis: 1.244 ± 0.022	1.946ProIle: 1.946 ± 0.028	2.768ProLys: 2.768 ± 0.039	4.652ProLeu: 4.652 ± 0.042	0.946ProMet: 0.946 ± 0.017	1.885ProAsn: 1.885 ± 0.027	4.249ProPro: 4.249 ± 0.083	2.298ProGln: 2.298 ± 0.032	2.571ProArg: 2.571 ± 0.029	4.997ProSer: 4.997 ± 0.059	2.656ProThr: 2.656 ± 0.033	3.747ProVal: 3.747 ± 0.04	0.557ProTrp: 0.557 ± 0.013	1.48ProTyr: 1.48 ± 0.025	0.001ProXaa: 0.001 ± 0.0
Gln	3.036GlnAla: 3.036 ± 0.034	0.949GlnCys: 0.949 ± 0.021	2.217GlnAsp: 2.217 ± 0.026	3.659GlnGlu: 3.659 ± 0.044	1.466GlnPhe: 1.466 ± 0.021	2.425GlnGly: 2.425 ± 0.036	1.297GlnHis: 1.297 ± 0.025	2.185GlnIle: 2.185 ± 0.028	3.157GlnLys: 3.157 ± 0.041	4.535GlnLeu: 4.535 ± 0.051	1.121GlnMet: 1.121 ± 0.02	2.021GlnAsn: 2.021 ± 0.03	2.343GlnPro: 2.343 ± 0.037	2.893GlnGln: 2.893 ± 0.061	2.645GlnArg: 2.645 ± 0.033	3.172GlnSer: 3.172 ± 0.037	2.357GlnThr: 2.357 ± 0.029	2.728GlnVal: 2.728 ± 0.034	0.528GlnTrp: 0.528 ± 0.014	1.284GlnTyr: 1.284 ± 0.021	0.002GlnXaa: 0.002 ± 0.001
Arg	3.01ArgAla: 3.01 ± 0.03	1.126ArgCys: 1.126 ± 0.025	2.627ArgAsp: 2.627 ± 0.037	3.79ArgGlu: 3.79 ± 0.04	1.991ArgPhe: 1.991 ± 0.024	2.817ArgGly: 2.817 ± 0.043	1.537ArgHis: 1.537 ± 0.024	2.641ArgIle: 2.641 ± 0.032	4.001ArgLys: 4.001 ± 0.049	5.126ArgLeu: 5.126 ± 0.05	1.175ArgMet: 1.175 ± 0.017	2.309ArgAsn: 2.309 ± 0.025	2.414ArgPro: 2.414 ± 0.033	2.487ArgGln: 2.487 ± 0.034	3.686ArgArg: 3.686 ± 0.041	4.027ArgSer: 4.027 ± 0.059	2.69ArgThr: 2.69 ± 0.029	3.033ArgVal: 3.033 ± 0.033	0.627ArgTrp: 0.627 ± 0.018	1.651ArgTyr: 1.651 ± 0.024	0.001ArgXaa: 0.001 ± 0.0
Ser	5.205SerAla: 5.205 ± 0.045	1.781SerCys: 1.781 ± 0.025	4.065SerAsp: 4.065 ± 0.047	5.12SerGlu: 5.12 ± 0.047	3.155SerPhe: 3.155 ± 0.034	4.95SerGly: 4.95 ± 0.05	1.988SerHis: 1.988 ± 0.03	3.557SerIle: 3.557 ± 0.038	4.503SerLys: 4.503 ± 0.042	8.099SerLeu: 8.099 ± 0.062	1.606SerMet: 1.606 ± 0.023	3.159SerAsn: 3.159 ± 0.035	5.182SerPro: 5.182 ± 0.07	3.623SerGln: 3.623 ± 0.043	4.123SerArg: 4.123 ± 0.052	9.014SerSer: 9.014 ± 0.097	4.521SerThr: 4.521 ± 0.047	5.118SerVal: 5.118 ± 0.047	0.959SerTrp: 0.959 ± 0.018	2.264SerTyr: 2.264 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	3.844ThrAla: 3.844 ± 0.037	1.293ThrCys: 1.293 ± 0.029	2.778ThrAsp: 2.778 ± 0.035	3.8ThrGlu: 3.8 ± 0.042	2.182ThrPhe: 2.182 ± 0.029	3.396ThrGly: 3.396 ± 0.043	1.237ThrHis: 1.237 ± 0.021	2.574ThrIle: 2.574 ± 0.035	2.899ThrLys: 2.899 ± 0.03	5.181ThrLeu: 5.181 ± 0.041	1.127ThrMet: 1.127 ± 0.02	1.949ThrAsn: 1.949 ± 0.025	3.135ThrPro: 3.135 ± 0.039	2.122ThrGln: 2.122 ± 0.029	2.338ThrArg: 2.338 ± 0.027	4.632ThrSer: 4.632 ± 0.053	2.931ThrThr: 2.931 ± 0.039	4.134ThrVal: 4.134 ± 0.046	0.664ThrTrp: 0.664 ± 0.016	1.571ThrTyr: 1.571 ± 0.024	0.002ThrXaa: 0.002 ± 0.001
Val	4.123ValAla: 4.123 ± 0.04	1.591ValCys: 1.591 ± 0.031	3.224ValAsp: 3.224 ± 0.03	4.027ValGlu: 4.027 ± 0.04	2.795ValPhe: 2.795 ± 0.031	3.388ValGly: 3.388 ± 0.034	1.602ValHis: 1.602 ± 0.022	3.337ValIle: 3.337 ± 0.033	3.874ValLys: 3.874 ± 0.037	6.303ValLeu: 6.303 ± 0.054	1.446ValMet: 1.446 ± 0.022	2.57ValAsn: 2.57 ± 0.036	3.431ValPro: 3.431 ± 0.038	2.819ValGln: 2.819 ± 0.034	3.056ValArg: 3.056 ± 0.033	5.051ValSer: 5.051 ± 0.045	3.92ValThr: 3.92 ± 0.039	4.373ValVal: 4.373 ± 0.04	0.736ValTrp: 0.736 ± 0.016	1.899ValTyr: 1.899 ± 0.029	0.001ValXaa: 0.001 ± 0.0
Trp	0.654TrpAla: 0.654 ± 0.015	0.251TrpCys: 0.251 ± 0.01	0.684TrpAsp: 0.684 ± 0.018	0.808TrpGlu: 0.808 ± 0.017	0.464TrpPhe: 0.464 ± 0.012	0.633TrpGly: 0.633 ± 0.016	0.301TrpHis: 0.301 ± 0.01	0.643TrpIle: 0.643 ± 0.014	0.892TrpLys: 0.892 ± 0.016	1.161TrpLeu: 1.161 ± 0.021	0.316TrpMet: 0.316 ± 0.01	0.717TrpAsn: 0.717 ± 0.016	0.438TrpPro: 0.438 ± 0.011	0.552TrpGln: 0.552 ± 0.014	0.672TrpArg: 0.672 ± 0.015	0.905TrpSer: 0.905 ± 0.018	0.646TrpThr: 0.646 ± 0.014	0.675TrpVal: 0.675 ± 0.016	0.197TrpTrp: 0.197 ± 0.009	0.378TrpTyr: 0.378 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.623TyrAla: 1.623 ± 0.025	0.789TyrCys: 0.789 ± 0.018	1.506TyrAsp: 1.506 ± 0.021	1.86TyrGlu: 1.86 ± 0.026	1.416TyrPhe: 1.416 ± 0.025	1.773TyrGly: 1.773 ± 0.026	0.801TyrHis: 0.801 ± 0.018	1.691TyrIle: 1.691 ± 0.025	1.746TyrLys: 1.746 ± 0.025	2.949TyrLeu: 2.949 ± 0.035	0.687TyrMet: 0.687 ± 0.015	1.316TyrAsn: 1.316 ± 0.023	1.39TyrPro: 1.39 ± 0.026	1.326TyrGln: 1.326 ± 0.024	1.735TyrArg: 1.735 ± 0.025	2.424TyrSer: 2.424 ± 0.03	1.673TyrThr: 1.673 ± 0.025	1.82TyrVal: 1.82 ± 0.023	0.424TyrTrp: 0.424 ± 0.017	1.135TyrTyr: 1.135 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.001XaaGlu: 0.001 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.002XaaGln: 0.002 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.064XaaXaa: 0.064 ± 0.011

Statistics based on 7979 proteins (3218414 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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