Amino acid dipepetide frequency for Aquisphaera giovannonii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.769AlaAla: 18.769 ± 0.152	1.293AlaCys: 1.293 ± 0.026	7.235AlaAsp: 7.235 ± 0.057	7.749AlaGlu: 7.749 ± 0.067	4.019AlaPhe: 4.019 ± 0.036	12.807AlaGly: 12.807 ± 0.089	1.948AlaHis: 1.948 ± 0.028	5.564AlaIle: 5.564 ± 0.05	3.141AlaLys: 3.141 ± 0.042	11.432AlaLeu: 11.432 ± 0.081	2.866AlaMet: 2.866 ± 0.036	2.434AlaAsn: 2.434 ± 0.041	6.631AlaPro: 6.631 ± 0.072	2.8AlaGln: 2.8 ± 0.031	10.794AlaArg: 10.794 ± 0.093	7.576AlaSer: 7.576 ± 0.078	6.442AlaThr: 6.442 ± 0.08	8.673AlaVal: 8.673 ± 0.074	2.0AlaTrp: 2.0 ± 0.033	2.532AlaTyr: 2.532 ± 0.033	0.0AlaXaa: 0.0 ± 0.0
Cys	0.712CysAla: 0.712 ± 0.019	0.143CysCys: 0.143 ± 0.008	0.531CysAsp: 0.531 ± 0.014	0.476CysGlu: 0.476 ± 0.014	0.299CysPhe: 0.299 ± 0.011	0.945CysGly: 0.945 ± 0.023	0.33CysHis: 0.33 ± 0.015	0.288CysIle: 0.288 ± 0.01	0.214CysLys: 0.214 ± 0.009	0.99CysLeu: 0.99 ± 0.019	0.149CysMet: 0.149 ± 0.008	0.2CysAsn: 0.2 ± 0.008	0.604CysPro: 0.604 ± 0.02	0.245CysGln: 0.245 ± 0.01	0.906CysArg: 0.906 ± 0.02	0.438CysSer: 0.438 ± 0.013	0.409CysThr: 0.409 ± 0.015	0.626CysVal: 0.626 ± 0.019	0.169CysTrp: 0.169 ± 0.009	0.237CysTyr: 0.237 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.383AspAla: 7.383 ± 0.061	0.415AspCys: 0.415 ± 0.014	3.609AspAsp: 3.609 ± 0.043	3.86AspGlu: 3.86 ± 0.04	1.935AspPhe: 1.935 ± 0.025	6.358AspGly: 6.358 ± 0.073	1.274AspHis: 1.274 ± 0.02	1.807AspIle: 1.807 ± 0.025	1.009AspLys: 1.009 ± 0.023	6.411AspLeu: 6.411 ± 0.061	0.846AspMet: 0.846 ± 0.018	0.987AspAsn: 0.987 ± 0.022	4.788AspPro: 4.788 ± 0.048	1.513AspGln: 1.513 ± 0.029	6.013AspArg: 6.013 ± 0.055	2.436AspSer: 2.436 ± 0.031	1.891AspThr: 1.891 ± 0.031	4.262AspVal: 4.262 ± 0.042	1.023AspTrp: 1.023 ± 0.026	1.348AspTyr: 1.348 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	8.362GluAla: 8.362 ± 0.067	0.419GluCys: 0.419 ± 0.012	2.961GluAsp: 2.961 ± 0.037	3.343GluGlu: 3.343 ± 0.043	1.713GluPhe: 1.713 ± 0.026	5.082GluGly: 5.082 ± 0.042	1.197GluHis: 1.197 ± 0.023	2.502GluIle: 2.502 ± 0.033	1.889GluLys: 1.889 ± 0.033	5.778GluLeu: 5.778 ± 0.056	1.123GluMet: 1.123 ± 0.02	1.076GluAsn: 1.076 ± 0.021	3.785GluPro: 3.785 ± 0.045	1.757GluGln: 1.757 ± 0.026	4.859GluArg: 4.859 ± 0.051	3.034GluSer: 3.034 ± 0.038	2.462GluThr: 2.462 ± 0.036	4.432GluVal: 4.432 ± 0.043	0.748GluTrp: 0.748 ± 0.017	1.158GluTyr: 1.158 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.769PheAla: 3.769 ± 0.04	0.315PheCys: 0.315 ± 0.012	2.289PheAsp: 2.289 ± 0.028	1.921PheGlu: 1.921 ± 0.024	1.103PhePhe: 1.103 ± 0.022	3.016PheGly: 3.016 ± 0.04	0.765PheHis: 0.765 ± 0.017	0.911PheIle: 0.911 ± 0.017	0.666PheLys: 0.666 ± 0.017	3.563PheLeu: 3.563 ± 0.044	0.446PheMet: 0.446 ± 0.012	0.843PheAsn: 0.843 ± 0.019	1.693PhePro: 1.693 ± 0.024	0.955PheGln: 0.955 ± 0.019	2.901PheArg: 2.901 ± 0.036	1.797PheSer: 1.797 ± 0.028	1.62PheThr: 1.62 ± 0.031	2.3PheVal: 2.3 ± 0.032	0.499PheTrp: 0.499 ± 0.013	0.802PheTyr: 0.802 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	8.979GlyAla: 8.979 ± 0.074	0.928GlyCys: 0.928 ± 0.02	5.331GlyAsp: 5.331 ± 0.054	5.239GlyGlu: 5.239 ± 0.047	3.018GlyPhe: 3.018 ± 0.038	9.5GlyGly: 9.5 ± 0.129	2.04GlyHis: 2.04 ± 0.03	3.601GlyIle: 3.601 ± 0.042	3.159GlyLys: 3.159 ± 0.044	9.657GlyLeu: 9.657 ± 0.073	2.164GlyMet: 2.164 ± 0.034	2.256GlyAsn: 2.256 ± 0.07	6.093GlyPro: 6.093 ± 0.061	2.741GlyGln: 2.741 ± 0.035	9.159GlyArg: 9.159 ± 0.08	5.499GlySer: 5.499 ± 0.072	5.356GlyThr: 5.356 ± 0.088	6.248GlyVal: 6.248 ± 0.05	1.686GlyTrp: 1.686 ± 0.028	2.21GlyTyr: 2.21 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.426HisAla: 2.426 ± 0.032	0.202HisCys: 0.202 ± 0.008	1.242HisAsp: 1.242 ± 0.021	1.155HisGlu: 1.155 ± 0.022	0.734HisPhe: 0.734 ± 0.016	1.914HisGly: 1.914 ± 0.027	0.567HisHis: 0.567 ± 0.016	0.608HisIle: 0.608 ± 0.016	0.388HisLys: 0.388 ± 0.011	2.052HisLeu: 2.052 ± 0.026	0.321HisMet: 0.321 ± 0.011	0.428HisAsn: 0.428 ± 0.013	1.635HisPro: 1.635 ± 0.027	0.506HisGln: 0.506 ± 0.013	1.821HisArg: 1.821 ± 0.027	0.871HisSer: 0.871 ± 0.018	0.68HisThr: 0.68 ± 0.015	1.383HisVal: 1.383 ± 0.022	0.334HisTrp: 0.334 ± 0.011	0.509HisTyr: 0.509 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.052IleAla: 5.052 ± 0.047	0.356IleCys: 0.356 ± 0.013	2.913IleAsp: 2.913 ± 0.035	2.619IleGlu: 2.619 ± 0.034	1.047IlePhe: 1.047 ± 0.021	3.674IleGly: 3.674 ± 0.045	0.85IleHis: 0.85 ± 0.018	1.176IleIle: 1.176 ± 0.027	1.044IleLys: 1.044 ± 0.021	4.064IleLeu: 4.064 ± 0.043	0.491IleMet: 0.491 ± 0.014	0.991IleAsn: 0.991 ± 0.019	2.553IlePro: 2.553 ± 0.027	1.104IleGln: 1.104 ± 0.019	3.594IleArg: 3.594 ± 0.039	1.927IleSer: 1.927 ± 0.03	1.922IleThr: 1.922 ± 0.028	3.05IleVal: 3.05 ± 0.034	0.513IleTrp: 0.513 ± 0.015	0.915IleTyr: 0.915 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	3.76LysAla: 3.76 ± 0.043	0.168LysCys: 0.168 ± 0.008	1.692LysAsp: 1.692 ± 0.031	1.511LysGlu: 1.511 ± 0.025	0.733LysPhe: 0.733 ± 0.017	2.353LysGly: 2.353 ± 0.029	0.49LysHis: 0.49 ± 0.014	1.092LysIle: 1.092 ± 0.022	1.054LysLys: 1.054 ± 0.026	2.592LysLeu: 2.592 ± 0.031	0.488LysMet: 0.488 ± 0.013	0.587LysAsn: 0.587 ± 0.016	2.003LysPro: 2.003 ± 0.031	0.775LysGln: 0.775 ± 0.02	1.7LysArg: 1.7 ± 0.026	1.408LysSer: 1.408 ± 0.022	1.305LysThr: 1.305 ± 0.023	2.248LysVal: 2.248 ± 0.03	0.288LysTrp: 0.288 ± 0.011	0.613LysTyr: 0.613 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.765LeuAla: 14.765 ± 0.092	0.949LeuCys: 0.949 ± 0.02	6.538LeuAsp: 6.538 ± 0.056	5.816LeuGlu: 5.816 ± 0.055	2.971LeuPhe: 2.971 ± 0.037	9.232LeuGly: 9.232 ± 0.074	1.817LeuHis: 1.817 ± 0.026	4.002LeuIle: 4.002 ± 0.039	3.008LeuLys: 3.008 ± 0.036	9.691LeuLeu: 9.691 ± 0.083	1.725LeuMet: 1.725 ± 0.032	2.13LeuAsn: 2.13 ± 0.029	5.843LeuPro: 5.843 ± 0.052	2.241LeuGln: 2.241 ± 0.033	8.365LeuArg: 8.365 ± 0.057	5.585LeuSer: 5.585 ± 0.047	4.887LeuThr: 4.887 ± 0.056	7.397LeuVal: 7.397 ± 0.058	1.287LeuTrp: 1.287 ± 0.022	1.992LeuTyr: 1.992 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.644MetAla: 2.644 ± 0.034	0.116MetCys: 0.116 ± 0.007	0.978MetAsp: 0.978 ± 0.02	0.977MetGlu: 0.977 ± 0.018	0.482MetPhe: 0.482 ± 0.015	1.666MetGly: 1.666 ± 0.031	0.314MetHis: 0.314 ± 0.011	0.925MetIle: 0.925 ± 0.021	0.677MetLys: 0.677 ± 0.017	1.813MetLeu: 1.813 ± 0.029	0.414MetMet: 0.414 ± 0.014	0.554MetAsn: 0.554 ± 0.014	1.397MetPro: 1.397 ± 0.024	0.476MetGln: 0.476 ± 0.012	1.413MetArg: 1.413 ± 0.026	1.253MetSer: 1.253 ± 0.023	1.259MetThr: 1.259 ± 0.018	1.276MetVal: 1.276 ± 0.024	0.166MetTrp: 0.166 ± 0.008	0.276MetTyr: 0.276 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.451AsnAla: 2.451 ± 0.041	0.206AsnCys: 0.206 ± 0.01	1.218AsnAsp: 1.218 ± 0.03	1.004AsnGlu: 1.004 ± 0.022	0.813AsnPhe: 0.813 ± 0.019	2.114AsnGly: 2.114 ± 0.044	0.448AsnHis: 0.448 ± 0.012	0.796AsnIle: 0.796 ± 0.019	0.452AsnLys: 0.452 ± 0.015	2.451AsnLeu: 2.451 ± 0.035	0.344AsnMet: 0.344 ± 0.01	0.674AsnAsn: 0.674 ± 0.022	1.772AsnPro: 1.772 ± 0.031	0.711AsnGln: 0.711 ± 0.017	1.768AsnArg: 1.768 ± 0.03	1.101AsnSer: 1.101 ± 0.024	1.085AsnThr: 1.085 ± 0.03	1.647AsnVal: 1.647 ± 0.033	0.348AsnTrp: 0.348 ± 0.011	0.652AsnTyr: 0.652 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	9.568ProAla: 9.568 ± 0.088	0.433ProCys: 0.433 ± 0.015	4.582ProAsp: 4.582 ± 0.051	4.405ProGlu: 4.405 ± 0.043	1.941ProPhe: 1.941 ± 0.029	7.368ProGly: 7.368 ± 0.068	1.126ProHis: 1.126 ± 0.022	2.491ProIle: 2.491 ± 0.025	1.551ProLys: 1.551 ± 0.028	5.108ProLeu: 5.108 ± 0.047	1.286ProMet: 1.286 ± 0.02	1.32ProAsn: 1.32 ± 0.025	4.752ProPro: 4.752 ± 0.061	1.434ProGln: 1.434 ± 0.029	5.033ProArg: 5.033 ± 0.053	4.232ProSer: 4.232 ± 0.046	3.077ProThr: 3.077 ± 0.037	4.187ProVal: 4.187 ± 0.04	0.946ProTrp: 0.946 ± 0.021	1.251ProTyr: 1.251 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.812GlnAla: 3.812 ± 0.039	0.207GlnCys: 0.207 ± 0.009	1.258GlnAsp: 1.258 ± 0.022	1.31GlnGlu: 1.31 ± 0.028	0.904GlnPhe: 0.904 ± 0.018	2.432GlnGly: 2.432 ± 0.035	0.49GlnHis: 0.49 ± 0.013	1.081GlnIle: 1.081 ± 0.02	0.747GlnLys: 0.747 ± 0.019	2.474GlnLeu: 2.474 ± 0.034	0.477GlnMet: 0.477 ± 0.014	0.586GlnAsn: 0.586 ± 0.016	1.715GlnPro: 1.715 ± 0.025	0.844GlnGln: 0.844 ± 0.021	1.762GlnArg: 1.762 ± 0.031	1.486GlnSer: 1.486 ± 0.025	1.191GlnThr: 1.191 ± 0.024	2.408GlnVal: 2.408 ± 0.035	0.363GlnTrp: 0.363 ± 0.013	0.59GlnTyr: 0.59 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.379ArgAla: 9.379 ± 0.076	0.758ArgCys: 0.758 ± 0.02	5.11ArgAsp: 5.11 ± 0.053	5.275ArgGlu: 5.275 ± 0.055	3.041ArgPhe: 3.041 ± 0.035	6.987ArgGly: 6.987 ± 0.059	1.835ArgHis: 1.835 ± 0.026	3.861ArgIle: 3.861 ± 0.039	2.398ArgLys: 2.398 ± 0.031	9.414ArgLeu: 9.414 ± 0.072	1.885ArgMet: 1.885 ± 0.027	1.684ArgAsn: 1.684 ± 0.027	6.288ArgPro: 6.288 ± 0.068	2.332ArgGln: 2.332 ± 0.036	8.818ArgArg: 8.818 ± 0.09	4.599ArgSer: 4.599 ± 0.043	3.733ArgThr: 3.733 ± 0.041	5.71ArgVal: 5.71 ± 0.048	1.539ArgTrp: 1.539 ± 0.022	1.981ArgTyr: 1.981 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	5.973SerAla: 5.973 ± 0.068	0.502SerCys: 0.502 ± 0.012	2.756SerAsp: 2.756 ± 0.032	2.424SerGlu: 2.424 ± 0.03	1.929SerPhe: 1.929 ± 0.029	5.683SerGly: 5.683 ± 0.068	1.081SerHis: 1.081 ± 0.02	2.36SerIle: 2.36 ± 0.033	1.352SerLys: 1.352 ± 0.025	5.901SerLeu: 5.901 ± 0.046	1.182SerMet: 1.182 ± 0.02	1.33SerAsn: 1.33 ± 0.029	4.452SerPro: 4.452 ± 0.042	1.494SerGln: 1.494 ± 0.025	4.83SerArg: 4.83 ± 0.047	3.511SerSer: 3.511 ± 0.048	2.864SerThr: 2.864 ± 0.051	3.686SerVal: 3.686 ± 0.047	0.944SerTrp: 0.944 ± 0.019	1.356SerTyr: 1.356 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	5.581ThrAla: 5.581 ± 0.076	0.423ThrCys: 0.423 ± 0.015	2.467ThrAsp: 2.467 ± 0.036	1.954ThrGlu: 1.954 ± 0.025	1.915ThrPhe: 1.915 ± 0.033	4.741ThrGly: 4.741 ± 0.076	0.873ThrHis: 0.873 ± 0.019	2.569ThrIle: 2.569 ± 0.039	1.094ThrLys: 1.094 ± 0.022	5.202ThrLeu: 5.202 ± 0.051	0.909ThrMet: 0.909 ± 0.019	1.172ThrAsn: 1.172 ± 0.028	3.686ThrPro: 3.686 ± 0.043	1.104ThrGln: 1.104 ± 0.022	3.392ThrArg: 3.392 ± 0.036	2.891ThrSer: 2.891 ± 0.048	2.794ThrThr: 2.794 ± 0.061	3.821ThrVal: 3.821 ± 0.063	0.825ThrTrp: 0.825 ± 0.021	1.24ThrTyr: 1.24 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	9.435ValAla: 9.435 ± 0.066	0.661ValCys: 0.661 ± 0.021	4.076ValAsp: 4.076 ± 0.038	4.613ValGlu: 4.613 ± 0.045	2.216ValPhe: 2.216 ± 0.03	5.914ValGly: 5.914 ± 0.054	1.436ValHis: 1.436 ± 0.024	2.885ValIle: 2.885 ± 0.037	1.944ValLys: 1.944 ± 0.031	7.4ValLeu: 7.4 ± 0.069	1.22ValMet: 1.22 ± 0.021	1.739ValAsn: 1.739 ± 0.034	4.355ValPro: 4.355 ± 0.038	1.874ValGln: 1.874 ± 0.028	5.981ValArg: 5.981 ± 0.05	3.81ValSer: 3.81 ± 0.044	3.79ValThr: 3.79 ± 0.06	6.179ValVal: 6.179 ± 0.056	0.869ValTrp: 0.869 ± 0.018	1.592ValTyr: 1.592 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	1.528TrpAla: 1.528 ± 0.026	0.176TrpCys: 0.176 ± 0.008	0.949TrpAsp: 0.949 ± 0.026	0.724TrpGlu: 0.724 ± 0.018	0.52TrpPhe: 0.52 ± 0.016	1.101TrpGly: 1.101 ± 0.024	0.346TrpHis: 0.346 ± 0.012	0.692TrpIle: 0.692 ± 0.016	0.557TrpLys: 0.557 ± 0.015	1.793TrpLeu: 1.793 ± 0.034	0.349TrpMet: 0.349 ± 0.011	0.419TrpAsn: 0.419 ± 0.014	0.83TrpPro: 0.83 ± 0.019	0.473TrpGln: 0.473 ± 0.013	1.289TrpArg: 1.289 ± 0.02	0.988TrpSer: 0.988 ± 0.02	0.862TrpThr: 0.862 ± 0.02	0.935TrpVal: 0.935 ± 0.018	0.281TrpTrp: 0.281 ± 0.011	0.383TrpTyr: 0.383 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.357TyrAla: 2.357 ± 0.031	0.241TyrCys: 0.241 ± 0.011	1.434TyrAsp: 1.434 ± 0.026	1.304TyrGlu: 1.304 ± 0.021	0.844TyrPhe: 0.844 ± 0.019	1.979TyrGly: 1.979 ± 0.033	0.546TyrHis: 0.546 ± 0.013	0.646TyrIle: 0.646 ± 0.015	0.515TyrLys: 0.515 ± 0.013	2.455TyrLeu: 2.455 ± 0.032	0.389TyrMet: 0.389 ± 0.011	0.645TyrAsn: 0.645 ± 0.017	1.217TyrPro: 1.217 ± 0.024	0.758TyrGln: 0.758 ± 0.016	2.227TyrArg: 2.227 ± 0.029	1.169TyrSer: 1.169 ± 0.023	1.023TyrThr: 1.023 ± 0.027	1.519TyrVal: 1.519 ± 0.024	0.369TyrTrp: 0.369 ± 0.012	0.7TyrTyr: 0.7 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7743 proteins (2971300 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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