Amino acid dipepetide frequency for Desulfotomaculum ruminis (strain ATCC 23193 / DSM 2154 / NCIMB 8452 / DL)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.338AlaAla: 8.338 ± 0.132	0.962AlaCys: 0.962 ± 0.042	3.699AlaAsp: 3.699 ± 0.061	5.628AlaGlu: 5.628 ± 0.086	2.942AlaPhe: 2.942 ± 0.061	7.334AlaGly: 7.334 ± 0.094	1.303AlaHis: 1.303 ± 0.034	5.189AlaIle: 5.189 ± 0.077	4.663AlaLys: 4.663 ± 0.068	8.815AlaLeu: 8.815 ± 0.108	2.328AlaMet: 2.328 ± 0.053	2.721AlaAsn: 2.721 ± 0.054	2.522AlaPro: 2.522 ± 0.052	2.948AlaGln: 2.948 ± 0.059	4.414AlaArg: 4.414 ± 0.074	4.045AlaSer: 4.045 ± 0.065	3.619AlaThr: 3.619 ± 0.058	6.955AlaVal: 6.955 ± 0.087	0.726AlaTrp: 0.726 ± 0.026	2.285AlaTyr: 2.285 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.799CysAla: 0.799 ± 0.03	0.242CysCys: 0.242 ± 0.016	0.488CysAsp: 0.488 ± 0.02	0.554CysGlu: 0.554 ± 0.023	0.446CysPhe: 0.446 ± 0.023	1.261CysGly: 1.261 ± 0.038	0.274CysHis: 0.274 ± 0.015	0.658CysIle: 0.658 ± 0.021	0.559CysLys: 0.559 ± 0.021	1.143CysLeu: 1.143 ± 0.033	0.264CysMet: 0.264 ± 0.016	0.417CysAsn: 0.417 ± 0.02	0.783CysPro: 0.783 ± 0.028	0.463CysGln: 0.463 ± 0.019	0.776CysArg: 0.776 ± 0.032	0.805CysSer: 0.805 ± 0.028	0.56CysThr: 0.56 ± 0.023	0.674CysVal: 0.674 ± 0.028	0.127CysTrp: 0.127 ± 0.011	0.38CysTyr: 0.38 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.248AspAla: 3.248 ± 0.06	0.57AspCys: 0.57 ± 0.023	1.916AspAsp: 1.916 ± 0.06	3.222AspGlu: 3.222 ± 0.055	2.259AspPhe: 2.259 ± 0.049	3.27AspGly: 3.27 ± 0.062	0.974AspHis: 0.974 ± 0.031	3.682AspIle: 3.682 ± 0.056	2.709AspLys: 2.709 ± 0.054	5.099AspLeu: 5.099 ± 0.066	1.252AspMet: 1.252 ± 0.033	1.553AspAsn: 1.553 ± 0.039	2.352AspPro: 2.352 ± 0.047	2.049AspGln: 2.049 ± 0.043	2.649AspArg: 2.649 ± 0.051	2.229AspSer: 2.229 ± 0.049	2.215AspThr: 2.215 ± 0.048	3.255AspVal: 3.255 ± 0.06	0.577AspTrp: 0.577 ± 0.025	1.877AspTyr: 1.877 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.808GluAla: 5.808 ± 0.079	0.555GluCys: 0.555 ± 0.027	3.165GluAsp: 3.165 ± 0.06	5.934GluGlu: 5.934 ± 0.086	2.214GluPhe: 2.214 ± 0.045	4.51GluGly: 4.51 ± 0.073	1.111GluHis: 1.111 ± 0.032	5.491GluIle: 5.491 ± 0.071	5.485GluLys: 5.485 ± 0.077	6.953GluLeu: 6.953 ± 0.094	2.253GluMet: 2.253 ± 0.048	2.913GluAsn: 2.913 ± 0.05	2.204GluPro: 2.204 ± 0.059	3.302GluGln: 3.302 ± 0.066	3.647GluArg: 3.647 ± 0.064	2.861GluSer: 2.861 ± 0.056	3.241GluThr: 3.241 ± 0.061	5.322GluVal: 5.322 ± 0.068	0.587GluTrp: 0.587 ± 0.023	2.02GluTyr: 2.02 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	2.953PheAla: 2.953 ± 0.054	0.519PheCys: 0.519 ± 0.02	1.837PheAsp: 1.837 ± 0.043	2.036PheGlu: 2.036 ± 0.044	1.876PhePhe: 1.876 ± 0.057	2.987PheGly: 2.987 ± 0.055	0.784PheHis: 0.784 ± 0.028	2.958PheIle: 2.958 ± 0.056	2.269PheLys: 2.269 ± 0.046	4.172PheLeu: 4.172 ± 0.077	1.069PheMet: 1.069 ± 0.034	1.702PheAsn: 1.702 ± 0.045	1.61PhePro: 1.61 ± 0.036	1.383PheGln: 1.383 ± 0.04	1.713PheArg: 1.713 ± 0.044	2.674PheSer: 2.674 ± 0.054	2.326PheThr: 2.326 ± 0.042	2.496PheVal: 2.496 ± 0.053	0.478PheTrp: 0.478 ± 0.022	1.415PheTyr: 1.415 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	5.805GlyAla: 5.805 ± 0.084	1.196GlyCys: 1.196 ± 0.032	3.45GlyAsp: 3.45 ± 0.057	4.913GlyGlu: 4.913 ± 0.074	3.264GlyPhe: 3.264 ± 0.066	5.688GlyGly: 5.688 ± 0.09	1.448GlyHis: 1.448 ± 0.033	5.719GlyIle: 5.719 ± 0.078	4.92GlyLys: 4.92 ± 0.077	8.212GlyLeu: 8.212 ± 0.097	2.315GlyMet: 2.315 ± 0.045	2.823GlyAsn: 2.823 ± 0.059	2.41GlyPro: 2.41 ± 0.054	3.073GlyGln: 3.073 ± 0.056	3.971GlyArg: 3.971 ± 0.067	4.334GlySer: 4.334 ± 0.059	4.121GlyThr: 4.121 ± 0.068	5.788GlyVal: 5.788 ± 0.083	0.866GlyTrp: 0.866 ± 0.03	2.857GlyTyr: 2.857 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.15HisAla: 1.15 ± 0.032	0.349HisCys: 0.349 ± 0.017	0.747HisAsp: 0.747 ± 0.029	0.988HisGlu: 0.988 ± 0.031	0.911HisPhe: 0.911 ± 0.03	1.382HisGly: 1.382 ± 0.036	0.54HisHis: 0.54 ± 0.024	1.241HisIle: 1.241 ± 0.033	0.923HisLys: 0.923 ± 0.028	2.075HisLeu: 2.075 ± 0.046	0.506HisMet: 0.506 ± 0.021	0.669HisAsn: 0.669 ± 0.026	1.152HisPro: 1.152 ± 0.032	0.808HisGln: 0.808 ± 0.028	1.145HisArg: 1.145 ± 0.035	1.031HisSer: 1.031 ± 0.032	0.893HisThr: 0.893 ± 0.027	0.968HisVal: 0.968 ± 0.031	0.253HisTrp: 0.253 ± 0.015	0.747HisTyr: 0.747 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.454IleAla: 5.454 ± 0.075	0.774IleCys: 0.774 ± 0.026	3.252IleAsp: 3.252 ± 0.059	4.078IleGlu: 4.078 ± 0.06	2.777IlePhe: 2.777 ± 0.056	4.969IleGly: 4.969 ± 0.082	1.427IleHis: 1.427 ± 0.041	4.889IleIle: 4.889 ± 0.076	4.327IleLys: 4.327 ± 0.069	7.336IleLeu: 7.336 ± 0.098	1.694IleMet: 1.694 ± 0.038	2.863IleAsn: 2.863 ± 0.047	3.269IlePro: 3.269 ± 0.06	2.577IleGln: 2.577 ± 0.055	3.422IleArg: 3.422 ± 0.063	4.299IleSer: 4.299 ± 0.07	4.141IleThr: 4.141 ± 0.063	4.329IleVal: 4.329 ± 0.069	0.585IleTrp: 0.585 ± 0.027	2.152IleTyr: 2.152 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.725LysAla: 4.725 ± 0.07	0.498LysCys: 0.498 ± 0.025	3.133LysAsp: 3.133 ± 0.062	5.469LysGlu: 5.469 ± 0.076	1.736LysPhe: 1.736 ± 0.036	4.214LysGly: 4.214 ± 0.061	0.933LysHis: 0.933 ± 0.026	4.636LysIle: 4.636 ± 0.067	4.424LysLys: 4.424 ± 0.077	5.452LysLeu: 5.452 ± 0.074	1.868LysMet: 1.868 ± 0.035	2.714LysAsn: 2.714 ± 0.05	2.44LysPro: 2.44 ± 0.047	2.374LysGln: 2.374 ± 0.045	2.825LysArg: 2.825 ± 0.053	2.884LysSer: 2.884 ± 0.063	3.161LysThr: 3.161 ± 0.051	4.515LysVal: 4.515 ± 0.072	0.531LysTrp: 0.531 ± 0.024	1.989LysTyr: 1.989 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	9.825LeuAla: 9.825 ± 0.107	1.169LeuCys: 1.169 ± 0.036	5.061LeuAsp: 5.061 ± 0.073	7.404LeuGlu: 7.404 ± 0.101	4.091LeuPhe: 4.091 ± 0.077	7.93LeuGly: 7.93 ± 0.095	1.8LeuHis: 1.8 ± 0.042	6.31LeuIle: 6.31 ± 0.095	6.594LeuLys: 6.594 ± 0.084	11.228LeuLeu: 11.228 ± 0.148	2.622LeuMet: 2.622 ± 0.049	4.121LeuAsn: 4.121 ± 0.067	4.882LeuPro: 4.882 ± 0.072	3.822LeuGln: 3.822 ± 0.072	4.825LeuArg: 4.825 ± 0.076	6.574LeuSer: 6.574 ± 0.084	5.722LeuThr: 5.722 ± 0.073	7.53LeuVal: 7.53 ± 0.103	1.056LeuTrp: 1.056 ± 0.033	2.89LeuTyr: 2.89 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.717MetAla: 2.717 ± 0.054	0.215MetCys: 0.215 ± 0.014	1.552MetAsp: 1.552 ± 0.038	2.114MetGlu: 2.114 ± 0.045	0.819MetPhe: 0.819 ± 0.027	2.175MetGly: 2.175 ± 0.045	0.366MetHis: 0.366 ± 0.019	1.8MetIle: 1.8 ± 0.045	1.821MetLys: 1.821 ± 0.045	2.625MetLeu: 2.625 ± 0.052	0.773MetMet: 0.773 ± 0.026	1.24MetAsn: 1.24 ± 0.033	1.112MetPro: 1.112 ± 0.035	0.903MetGln: 0.903 ± 0.03	1.134MetArg: 1.134 ± 0.028	1.507MetSer: 1.507 ± 0.041	1.444MetThr: 1.444 ± 0.038	2.242MetVal: 2.242 ± 0.044	0.162MetTrp: 0.162 ± 0.012	0.551MetTyr: 0.551 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.509AsnAla: 2.509 ± 0.05	0.505AsnCys: 0.505 ± 0.023	1.568AsnAsp: 1.568 ± 0.04	2.043AsnGlu: 2.043 ± 0.047	1.489AsnPhe: 1.489 ± 0.038	2.7AsnGly: 2.7 ± 0.056	0.895AsnHis: 0.895 ± 0.029	2.98AsnIle: 2.98 ± 0.065	2.339AsnLys: 2.339 ± 0.054	4.293AsnLeu: 4.293 ± 0.061	0.983AsnMet: 0.983 ± 0.025	1.582AsnAsn: 1.582 ± 0.044	2.315AsnPro: 2.315 ± 0.048	1.825AsnGln: 1.825 ± 0.048	2.329AsnArg: 2.329 ± 0.047	1.953AsnSer: 1.953 ± 0.043	1.959AsnThr: 1.959 ± 0.042	2.325AsnVal: 2.325 ± 0.049	0.476AsnTrp: 0.476 ± 0.021	1.38AsnTyr: 1.38 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	3.315ProAla: 3.315 ± 0.072	0.447ProCys: 0.447 ± 0.023	2.287ProAsp: 2.287 ± 0.05	3.936ProGlu: 3.936 ± 0.064	1.728ProPhe: 1.728 ± 0.04	3.902ProGly: 3.902 ± 0.064	0.793ProHis: 0.793 ± 0.024	2.171ProIle: 2.171 ± 0.044	1.955ProLys: 1.955 ± 0.046	4.209ProLeu: 4.209 ± 0.067	1.011ProMet: 1.011 ± 0.027	1.384ProAsn: 1.384 ± 0.033	1.607ProPro: 1.607 ± 0.042	1.635ProGln: 1.635 ± 0.04	1.862ProArg: 1.862 ± 0.047	2.157ProSer: 2.157 ± 0.047	1.955ProThr: 1.955 ± 0.048	4.105ProVal: 4.105 ± 0.067	0.488ProTrp: 0.488 ± 0.022	1.33ProTyr: 1.33 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	3.466GlnAla: 3.466 ± 0.061	0.408GlnCys: 0.408 ± 0.019	1.78GlnAsp: 1.78 ± 0.036	3.433GlnGlu: 3.433 ± 0.065	1.306GlnPhe: 1.306 ± 0.037	3.108GlnGly: 3.108 ± 0.075	0.619GlnHis: 0.619 ± 0.022	2.596GlnIle: 2.596 ± 0.045	2.647GlnLys: 2.647 ± 0.053	3.723GlnLeu: 3.723 ± 0.062	1.222GlnMet: 1.222 ± 0.034	1.51GlnAsn: 1.51 ± 0.038	1.603GlnPro: 1.603 ± 0.043	1.983GlnGln: 1.983 ± 0.051	2.165GlnArg: 2.165 ± 0.04	1.911GlnSer: 1.911 ± 0.041	1.825GlnThr: 1.825 ± 0.048	3.085GlnVal: 3.085 ± 0.058	0.511GlnTrp: 0.511 ± 0.02	1.245GlnTyr: 1.245 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	3.52ArgAla: 3.52 ± 0.066	0.618ArgCys: 0.618 ± 0.023	2.495ArgAsp: 2.495 ± 0.051	4.353ArgGlu: 4.353 ± 0.066	1.972ArgPhe: 1.972 ± 0.042	3.348ArgGly: 3.348 ± 0.056	0.961ArgHis: 0.961 ± 0.032	3.503ArgIle: 3.503 ± 0.059	3.065ArgLys: 3.065 ± 0.055	5.438ArgLeu: 5.438 ± 0.084	1.479ArgMet: 1.479 ± 0.043	1.99ArgAsn: 1.99 ± 0.037	1.937ArgPro: 1.937 ± 0.045	2.518ArgGln: 2.518 ± 0.048	2.878ArgArg: 2.878 ± 0.065	2.56ArgSer: 2.56 ± 0.047	2.339ArgThr: 2.339 ± 0.044	3.781ArgVal: 3.781 ± 0.061	0.606ArgTrp: 0.606 ± 0.021	1.775ArgTyr: 1.775 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.989SerAla: 3.989 ± 0.069	0.662SerCys: 0.662 ± 0.029	2.298SerAsp: 2.298 ± 0.051	3.106SerGlu: 3.106 ± 0.059	2.442SerPhe: 2.442 ± 0.046	4.926SerGly: 4.926 ± 0.07	1.067SerHis: 1.067 ± 0.031	3.659SerIle: 3.659 ± 0.062	2.871SerLys: 2.871 ± 0.049	6.387SerLeu: 6.387 ± 0.092	1.503SerMet: 1.503 ± 0.037	1.857SerAsn: 1.857 ± 0.045	2.555SerPro: 2.555 ± 0.047	2.235SerGln: 2.235 ± 0.048	2.989SerArg: 2.989 ± 0.047	3.299SerSer: 3.299 ± 0.078	2.756SerThr: 2.756 ± 0.05	3.62SerVal: 3.62 ± 0.059	0.599SerTrp: 0.599 ± 0.022	1.753SerTyr: 1.753 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.443ThrAla: 4.443 ± 0.066	0.553ThrCys: 0.553 ± 0.023	2.394ThrAsp: 2.394 ± 0.05	3.008ThrGlu: 3.008 ± 0.057	2.026ThrPhe: 2.026 ± 0.044	5.088ThrGly: 5.088 ± 0.074	0.951ThrHis: 0.951 ± 0.03	3.377ThrIle: 3.377 ± 0.067	2.395ThrLys: 2.395 ± 0.047	5.643ThrLeu: 5.643 ± 0.09	1.208ThrMet: 1.208 ± 0.032	1.72ThrAsn: 1.72 ± 0.042	2.532ThrPro: 2.532 ± 0.046	1.545ThrGln: 1.545 ± 0.036	2.465ThrArg: 2.465 ± 0.049	2.749ThrSer: 2.749 ± 0.055	2.678ThrThr: 2.678 ± 0.063	4.334ThrVal: 4.334 ± 0.064	0.494ThrTrp: 0.494 ± 0.025	1.479ThrTyr: 1.479 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	6.366ValAla: 6.366 ± 0.079	0.856ValCys: 0.856 ± 0.034	3.757ValAsp: 3.757 ± 0.067	4.911ValGlu: 4.911 ± 0.066	2.998ValPhe: 2.998 ± 0.058	5.129ValGly: 5.129 ± 0.079	1.286ValHis: 1.286 ± 0.036	5.097ValIle: 5.097 ± 0.075	4.377ValLys: 4.377 ± 0.063	7.841ValLeu: 7.841 ± 0.089	1.964ValMet: 1.964 ± 0.039	2.939ValAsn: 2.939 ± 0.047	3.098ValPro: 3.098 ± 0.057	2.599ValGln: 2.599 ± 0.052	3.476ValArg: 3.476 ± 0.058	4.381ValSer: 4.381 ± 0.066	4.091ValThr: 4.091 ± 0.067	5.883ValVal: 5.883 ± 0.081	0.667ValTrp: 0.667 ± 0.028	2.122ValTyr: 2.122 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.733TrpAla: 0.733 ± 0.025	0.132TrpCys: 0.132 ± 0.012	0.522TrpAsp: 0.522 ± 0.024	0.71TrpGlu: 0.71 ± 0.028	0.447TrpPhe: 0.447 ± 0.019	0.763TrpGly: 0.763 ± 0.028	0.209TrpHis: 0.209 ± 0.015	0.625TrpIle: 0.625 ± 0.026	0.526TrpLys: 0.526 ± 0.024	1.191TrpLeu: 1.191 ± 0.036	0.277TrpMet: 0.277 ± 0.017	0.412TrpAsn: 0.412 ± 0.021	0.395TrpPro: 0.395 ± 0.019	0.605TrpGln: 0.605 ± 0.024	0.553TrpArg: 0.553 ± 0.021	0.571TrpSer: 0.571 ± 0.022	0.453TrpThr: 0.453 ± 0.021	0.726TrpVal: 0.726 ± 0.026	0.154TrpTrp: 0.154 ± 0.013	0.316TrpTyr: 0.316 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.274TyrAla: 2.274 ± 0.047	0.442TyrCys: 0.442 ± 0.021	1.557TyrAsp: 1.557 ± 0.047	1.736TyrGlu: 1.736 ± 0.039	1.514TyrPhe: 1.514 ± 0.042	2.485TyrGly: 2.485 ± 0.047	0.738TyrHis: 0.738 ± 0.027	2.037TyrIle: 2.037 ± 0.046	1.652TyrLys: 1.652 ± 0.046	3.623TyrLeu: 3.623 ± 0.061	0.678TyrMet: 0.678 ± 0.026	1.318TyrAsn: 1.318 ± 0.042	1.5TyrPro: 1.5 ± 0.036	1.498TyrGln: 1.498 ± 0.041	1.896TyrArg: 1.896 ± 0.044	1.75TyrSer: 1.75 ± 0.041	1.583TyrThr: 1.583 ± 0.048	1.906TyrVal: 1.906 ± 0.039	0.375TyrTrp: 0.375 ± 0.02	1.205TyrTyr: 1.205 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3763 proteins (1098365 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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