Streptomyces cyaneogriseus subsp. noncyanogenus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces cyaneogriseus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5829 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5FX28|A0A0C5FX28_9ACTN Uncharacterized protein OS=Streptomyces cyaneogriseus subsp. noncyanogenus OX=477245 GN=TU94_12950 PE=4 SV=1
MM1 pKa = 7.83TDD3 pKa = 3.35DD4 pKa = 3.32QHH6 pKa = 9.03AEE8 pKa = 3.86GMGQGGGRR16 pKa = 11.84WDD18 pKa = 4.35PLPQGDD24 pKa = 4.33YY25 pKa = 11.28DD26 pKa = 4.37DD27 pKa = 4.93GATAFVKK34 pKa = 10.6LPEE37 pKa = 4.6GGIDD41 pKa = 3.79ALLASADD48 pKa = 3.98SPLAAPGHH56 pKa = 6.36GYY58 pKa = 9.65VPPRR62 pKa = 11.84ITVAPTTPAATDD74 pKa = 3.65PAAPGPWGAPDD85 pKa = 5.81GGAQWHH91 pKa = 6.68APDD94 pKa = 4.6APEE97 pKa = 4.95GGYY100 pKa = 8.76PHH102 pKa = 7.14QDD104 pKa = 2.45TGYY107 pKa = 9.15GQHH110 pKa = 6.25GQDD113 pKa = 3.73AGYY116 pKa = 8.59PQDD119 pKa = 3.38GGYY122 pKa = 10.43AQGGDD127 pKa = 3.27GGYY130 pKa = 9.04PHH132 pKa = 6.99QGQDD136 pKa = 2.85AGYY139 pKa = 8.33GQHH142 pKa = 6.55GRR144 pKa = 11.84DD145 pKa = 2.92AGYY148 pKa = 8.94PQGFDD153 pKa = 2.87GGYY156 pKa = 9.52EE157 pKa = 3.83QDD159 pKa = 3.61PEE161 pKa = 4.31AGYY164 pKa = 10.19RR165 pKa = 11.84QGEE168 pKa = 4.35DD169 pKa = 2.29AGYY172 pKa = 9.27PYY174 pKa = 10.46GAPAGYY180 pKa = 9.14RR181 pKa = 11.84QHH183 pKa = 7.23DD184 pKa = 4.2PYY186 pKa = 11.43GVAGRR191 pKa = 11.84QDD193 pKa = 3.22GAQYY197 pKa = 10.63DD198 pKa = 4.02PHH200 pKa = 8.07AGPQYY205 pKa = 10.25VADD208 pKa = 4.72DD209 pKa = 3.93RR210 pKa = 11.84FTYY213 pKa = 10.45HH214 pKa = 7.33PGATGQWDD222 pKa = 3.84YY223 pKa = 11.81TEE225 pKa = 5.31AAAQEE230 pKa = 4.25DD231 pKa = 4.38TAAAAPAPAPGHH243 pKa = 6.77DD244 pKa = 3.67VTGQWSIPVAGGDD257 pKa = 3.84LPDD260 pKa = 3.81EE261 pKa = 4.7SGEE264 pKa = 4.14FTTSSLVEE272 pKa = 4.05QWGGTPPATLPGGASAPWAPPAAGPQAHH300 pKa = 6.89GDD302 pKa = 3.59VDD304 pKa = 3.89PAADD308 pKa = 3.76PFAGHH313 pKa = 7.39GGRR316 pKa = 11.84QDD318 pKa = 3.86DD319 pKa = 4.1APVAPEE325 pKa = 4.55HH326 pKa = 6.61GFAPYY331 pKa = 10.84AEE333 pKa = 4.74DD334 pKa = 3.17AGEE337 pKa = 4.09AVEE340 pKa = 4.33QPEE343 pKa = 4.77PEE345 pKa = 4.21PEE347 pKa = 4.49GPGVPQEE354 pKa = 4.37PGQEE358 pKa = 4.14SEE360 pKa = 5.05APGTPEE366 pKa = 3.94GQLPAQDD373 pKa = 3.53SVEE376 pKa = 4.16PAEE379 pKa = 4.43EE380 pKa = 4.09TSPPAAAPEE389 pKa = 4.3APEE392 pKa = 4.39GWQGAEE398 pKa = 4.13QPDD401 pKa = 4.46GSPEE405 pKa = 4.22TPSEE409 pKa = 4.1AQDD412 pKa = 3.8GPEE415 pKa = 4.39APSPAQDD422 pKa = 3.34APEE425 pKa = 3.98AAEE428 pKa = 4.18AQDD431 pKa = 3.58AVEE434 pKa = 5.05DD435 pKa = 4.07VPEE438 pKa = 4.33ATDD441 pKa = 3.85APDD444 pKa = 3.25MPEE447 pKa = 4.17DD448 pKa = 3.75SGAGEE453 pKa = 4.34PLDD456 pKa = 4.97ARR458 pKa = 11.84DD459 pKa = 3.75GQEE462 pKa = 3.8AQHH465 pKa = 6.27AHH467 pKa = 7.19DD468 pKa = 4.75GQHH471 pKa = 7.03AYY473 pKa = 10.13DD474 pKa = 4.06AQHH477 pKa = 6.95APGEE481 pKa = 4.36HH482 pKa = 6.89DD483 pKa = 4.39AVPAPEE489 pKa = 5.03GPAEE493 pKa = 4.3PFAAPAAAHH502 pKa = 6.83DD503 pKa = 4.1AQAAPEE509 pKa = 4.2AEE511 pKa = 4.43EE512 pKa = 4.91APEE515 pKa = 4.06APEE518 pKa = 3.83TSEE521 pKa = 3.96TSGTSEE527 pKa = 4.03TPEE530 pKa = 4.02AATGPGAPADD540 pKa = 4.11PADD543 pKa = 4.63GPDD546 pKa = 3.64PAAGTDD552 pKa = 3.63PADD555 pKa = 4.25PSAGTAPAPGDD566 pKa = 4.09EE567 pKa = 4.72PPAGAPAEE575 pKa = 4.26PEE577 pKa = 4.24EE578 pKa = 4.52EE579 pKa = 4.09PAAAPAAHH587 pKa = 7.33DD588 pKa = 3.78EE589 pKa = 4.64HH590 pKa = 7.23PLASYY595 pKa = 9.26VLRR598 pKa = 11.84VNGTDD603 pKa = 4.45RR604 pKa = 11.84PVTDD608 pKa = 2.98AWIGEE613 pKa = 4.15SLLYY617 pKa = 10.18VLRR620 pKa = 11.84EE621 pKa = 3.94RR622 pKa = 11.84LGLAGAKK629 pKa = 9.72DD630 pKa = 3.75GCSQGEE636 pKa = 4.12CGACNVQVDD645 pKa = 3.94GRR647 pKa = 11.84LVASCLVPAATAAGSEE663 pKa = 4.08VRR665 pKa = 11.84TVEE668 pKa = 4.06GLAADD673 pKa = 4.65GQPSDD678 pKa = 3.75VQRR681 pKa = 11.84ALARR685 pKa = 11.84CGAVQCGFCVPGMAMTVHH703 pKa = 7.27DD704 pKa = 5.16LLEE707 pKa = 4.78GNPAPTEE714 pKa = 4.03LEE716 pKa = 3.87ARR718 pKa = 11.84QALCGNLCRR727 pKa = 11.84CSGYY731 pKa = 10.32RR732 pKa = 11.84GVLRR736 pKa = 11.84AVEE739 pKa = 4.13EE740 pKa = 4.62VVAEE744 pKa = 4.72RR745 pKa = 11.84GAHH748 pKa = 5.08SAADD752 pKa = 3.47ASEE755 pKa = 4.32ADD757 pKa = 3.68GGRR760 pKa = 11.84TRR762 pKa = 11.84VPHH765 pKa = 5.19QAGPEE770 pKa = 3.86AGGAEE775 pKa = 4.57PSPFDD780 pKa = 3.93PPEE783 pKa = 4.81APGPHH788 pKa = 6.54DD789 pKa = 3.5QPYY792 pKa = 9.54GQDD795 pKa = 3.28GGHH798 pKa = 6.65AA799 pKa = 3.82

Molecular weight: 80.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5FVJ8|A0A0C5FVJ8_9ACTN Regulatory protein OS=Streptomyces cyaneogriseus subsp. noncyanogenus OX=477245 GN=TU94_09590 PE=4 SV=1
MM1 pKa = 7.47ARR3 pKa = 11.84TAVRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84PPAPYY14 pKa = 9.67RR15 pKa = 11.84RR16 pKa = 11.84PAPALRR22 pKa = 11.84PSPPRR27 pKa = 11.84PARR30 pKa = 11.84ARR32 pKa = 11.84PGRR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84ARR40 pKa = 11.84GQPSPACRR48 pKa = 11.84PGVRR52 pKa = 11.84TSSSTLSTVSS62 pKa = 3.01

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5829

0

5829

1944252

29

6673

333.5

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.983 ± 0.053

0.794 ± 0.008

5.944 ± 0.026

5.881 ± 0.035

2.683 ± 0.019

9.652 ± 0.038

2.305 ± 0.015

2.999 ± 0.023

2.053 ± 0.029

10.278 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.685 ± 0.014

1.639 ± 0.016

6.228 ± 0.033

2.621 ± 0.018

8.452 ± 0.041

4.673 ± 0.025

6.015 ± 0.026

8.492 ± 0.031

1.509 ± 0.016

2.112 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski