Erwinia phage PhiEaH1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Iapetusvirus; Erwinia virus EaH1

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8CZN9|W8CZN9_9CAUD Uncharacterized protein OS=Erwinia phage PhiEaH1 OX=1401669 PE=4 SV=1
MM1 pKa = 8.14AEE3 pKa = 4.19EE4 pKa = 4.05IATGVVEE11 pKa = 4.33IGNGRR16 pKa = 11.84FLVNDD21 pKa = 3.26EE22 pKa = 4.27TAYY25 pKa = 9.6DD26 pKa = 4.05TACVIWMNRR35 pKa = 11.84VYY37 pKa = 11.13GEE39 pKa = 4.14GDD41 pKa = 3.34EE42 pKa = 5.01GPDD45 pKa = 2.48RR46 pKa = 11.84VVEE49 pKa = 4.22TRR51 pKa = 11.84PTRR54 pKa = 11.84YY55 pKa = 8.97PCEE58 pKa = 3.97VLCQHH63 pKa = 6.22EE64 pKa = 4.36NEE66 pKa = 4.39EE67 pKa = 4.78DD68 pKa = 3.49GTVAIFTAINVV79 pKa = 3.58

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8D0I4|W8D0I4_9CAUD Putative DNA-directed RNA polymerase beta subunit OS=Erwinia phage PhiEaH1 OX=1401669 PE=4 SV=1
MM1 pKa = 7.72VIFLNLDD8 pKa = 3.64GLNKK12 pKa = 9.95EE13 pKa = 4.62LPDD16 pKa = 3.57LLRR19 pKa = 11.84RR20 pKa = 11.84VLLLFFQVIHH30 pKa = 5.86QPPLTAPLRR39 pKa = 11.84HH40 pKa = 6.26RR41 pKa = 11.84SHH43 pKa = 6.1GRR45 pKa = 11.84FRR47 pKa = 11.84EE48 pKa = 3.8HH49 pKa = 5.98RR50 pKa = 11.84TLVRR54 pKa = 11.84DD55 pKa = 3.63SVEE58 pKa = 4.08GVTPQPVIVPGTGCSVFQVVAGHH81 pKa = 6.26NGIAADD87 pKa = 4.28TIKK90 pKa = 10.78NGHH93 pKa = 7.16PSTDD97 pKa = 3.06TRR99 pKa = 11.84NKK101 pKa = 9.97FVFRR105 pKa = 11.84LHH107 pKa = 7.09RR108 pKa = 11.84SLNLARR114 pKa = 11.84GEE116 pKa = 4.19HH117 pKa = 6.37LNCLVGGNVVRR128 pKa = 11.84DD129 pKa = 3.65PGLGVKK135 pKa = 9.52FQSLQHH141 pKa = 5.15VRR143 pKa = 11.84RR144 pKa = 11.84LTQEE148 pKa = 4.12VTQHH152 pKa = 6.14FYY154 pKa = 11.56FMVHH158 pKa = 6.61CAPHH162 pKa = 6.5LRR164 pKa = 11.84SKK166 pKa = 11.22DD167 pKa = 3.24RR168 pKa = 11.84FHH170 pKa = 6.76FTDD173 pKa = 4.79FVARR177 pKa = 11.84VHH179 pKa = 6.7DD180 pKa = 3.94CRR182 pKa = 11.84IKK184 pKa = 10.56AFRR187 pKa = 11.84HH188 pKa = 4.12VTTGYY193 pKa = 10.13LRR195 pKa = 11.84RR196 pKa = 11.84GVLCPEE202 pKa = 4.66LYY204 pKa = 9.16VTEE207 pKa = 4.35RR208 pKa = 11.84NPRR211 pKa = 11.84YY212 pKa = 8.88PVRR215 pKa = 11.84DD216 pKa = 3.34VFRR219 pKa = 11.84YY220 pKa = 9.82LLQDD224 pKa = 3.2FTDD227 pKa = 3.55RR228 pKa = 11.84TTTVSHH234 pKa = 7.11HH235 pKa = 6.13ARR237 pKa = 11.84NLRR240 pKa = 11.84NNHH243 pKa = 4.83FPYY246 pKa = 10.28RR247 pKa = 11.84RR248 pKa = 11.84RR249 pKa = 3.47

Molecular weight: 29.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

240

1

241

68675

35

2222

285.0

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.588 ± 0.168

0.813 ± 0.055

6.324 ± 0.111

6.32 ± 0.182

4.066 ± 0.117

7.061 ± 0.271

2.001 ± 0.092

5.12 ± 0.097

5.257 ± 0.16

9.009 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.879 ± 0.082

4.961 ± 0.103

4.229 ± 0.099

3.85 ± 0.098

5.804 ± 0.15

5.832 ± 0.137

6.44 ± 0.151

7.161 ± 0.14

1.389 ± 0.066

3.898 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski