Acinetobacter phage AbKT21phiIII

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Beijerinckvirinae; Friunavirus; Acinetobacter virus AbKT21III

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3T0IGI5|A0A3T0IGI5_9CAUD Capsid and scaffold protein OS=Acinetobacter phage AbKT21phiIII OX=2500826 PE=4 SV=1
MM1 pKa = 7.12TNYY4 pKa = 9.44VVKK7 pKa = 10.55NAEE10 pKa = 4.23LTTEE14 pKa = 3.85QHH16 pKa = 7.2DD17 pKa = 3.77RR18 pKa = 11.84LQGKK22 pKa = 8.83GYY24 pKa = 10.71KK25 pKa = 9.57FDD27 pKa = 3.72NTNGEE32 pKa = 4.39DD33 pKa = 3.58LTGGNHH39 pKa = 7.18FIVSDD44 pKa = 4.69RR45 pKa = 11.84INEE48 pKa = 3.99YY49 pKa = 9.23STNANNLCLLTCGGFEE65 pKa = 4.63LYY67 pKa = 10.62EE68 pKa = 4.38SVDD71 pKa = 3.38QLIDD75 pKa = 3.4VLPNVVGFVVGARR88 pKa = 11.84VKK90 pKa = 10.92LNNLQGNPKK99 pKa = 8.46MFNYY103 pKa = 8.15ATWSEE108 pKa = 4.0AGFEE112 pKa = 4.14LGAAGTIIAEE122 pKa = 3.77VDD124 pKa = 3.58YY125 pKa = 11.54AGDD128 pKa = 3.48VYY130 pKa = 11.58VRR132 pKa = 11.84FDD134 pKa = 3.61TASHH138 pKa = 7.41PDD140 pKa = 3.52RR141 pKa = 11.84DD142 pKa = 4.83DD143 pKa = 3.83KK144 pKa = 11.34EE145 pKa = 4.33LWVAVADD152 pKa = 5.56LILEE156 pKa = 4.23

Molecular weight:
17.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3T0IGH8|A0A3T0IGH8_9CAUD Uncharacterized protein OS=Acinetobacter phage AbKT21phiIII OX=2500826 PE=4 SV=1
MM1 pKa = 7.24LTDD4 pKa = 5.66CIDD7 pKa = 3.49HH8 pKa = 6.82GKK10 pKa = 7.95TKK12 pKa = 10.55SLSPEE17 pKa = 4.14GYY19 pKa = 10.67VMVLNPYY26 pKa = 9.32KK27 pKa = 10.48KK28 pKa = 9.45PRR30 pKa = 11.84ISRR33 pKa = 11.84LHH35 pKa = 6.57RR36 pKa = 11.84IVYY39 pKa = 8.27CQSNNIHH46 pKa = 5.74MQDD49 pKa = 2.93IEE51 pKa = 4.52GLVVRR56 pKa = 11.84HH57 pKa = 5.98KK58 pKa = 10.65CDD60 pKa = 3.02NPRR63 pKa = 11.84CVNPEE68 pKa = 3.68HH69 pKa = 8.13LEE71 pKa = 4.01IGTLADD77 pKa = 3.68NNKK80 pKa = 9.7DD81 pKa = 3.07RR82 pKa = 11.84AKK84 pKa = 10.37RR85 pKa = 11.84GRR87 pKa = 11.84STKK90 pKa = 10.08VVPSKK95 pKa = 11.01HH96 pKa = 6.41KK97 pKa = 10.03LTTEE101 pKa = 4.76DD102 pKa = 2.87ISTIRR107 pKa = 11.84SRR109 pKa = 11.84YY110 pKa = 8.98IKK112 pKa = 10.66GKK114 pKa = 9.46AGKK117 pKa = 9.62YY118 pKa = 9.36NPNGYY123 pKa = 9.71AALAKK128 pKa = 9.97EE129 pKa = 4.26YY130 pKa = 10.96NVDD133 pKa = 3.19EE134 pKa = 4.22KK135 pKa = 11.47VIYY138 pKa = 10.59NVVKK142 pKa = 10.78HH143 pKa = 6.08KK144 pKa = 10.75GRR146 pKa = 11.84YY147 pKa = 7.55AA148 pKa = 3.33

Molecular weight:
16.92 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

11546

65

1032

274.9

30.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.159 ± 0.357

0.979 ± 0.171

6.253 ± 0.216

6.167 ± 0.268

3.386 ± 0.146

7.327 ± 0.252

2.044 ± 0.175

5.604 ± 0.245

6.643 ± 0.292

8.54 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.159

4.954 ± 0.29

3.698 ± 0.177

4.651 ± 0.273

4.833 ± 0.251

5.699 ± 0.327

6.037 ± 0.308

6.773 ± 0.283

1.169 ± 0.104

4.46 ± 0.236

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski