Pachysolen tannophilus NRRL Y-2460

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Pachysolen; Pachysolen tannophilus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5645 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E4U1I7|A0A1E4U1I7_PACTA Uncharacterized protein OS=Pachysolen tannophilus NRRL Y-2460 OX=669874 GN=PACTADRAFT_47700 PE=4 SV=1
MM1 pKa = 7.37LVDD4 pKa = 4.23KK5 pKa = 10.71KK6 pKa = 11.13LIAAVVALASATVSAKK22 pKa = 10.63GSFGFDD28 pKa = 2.94LGVVDD33 pKa = 6.14NDD35 pKa = 4.9DD36 pKa = 3.65NCKK39 pKa = 10.72DD40 pKa = 3.56ADD42 pKa = 4.8DD43 pKa = 5.14YY44 pKa = 11.7IADD47 pKa = 4.31LDD49 pKa = 4.79AISGYY54 pKa = 7.59TSHH57 pKa = 6.43VKK59 pKa = 9.61TYY61 pKa = 10.93AVSDD65 pKa = 4.28CNTLQILGPVLEE77 pKa = 5.28DD78 pKa = 2.95KK79 pKa = 11.33GFTITLGVWPTDD91 pKa = 3.42DD92 pKa = 3.27NHH94 pKa = 7.02FDD96 pKa = 3.75EE97 pKa = 6.13EE98 pKa = 4.3ISALQSYY105 pKa = 10.24LPTISKK111 pKa = 10.46SVISEE116 pKa = 3.76ITVGSEE122 pKa = 3.28VLYY125 pKa = 10.98RR126 pKa = 11.84GDD128 pKa = 3.37LTADD132 pKa = 3.3QLASKK137 pKa = 8.4ITQVKK142 pKa = 8.4STISSITDD150 pKa = 3.35KK151 pKa = 11.6NGDD154 pKa = 3.99SYY156 pKa = 12.11SDD158 pKa = 3.48IPVGTVDD165 pKa = 3.65SWNCLVDD172 pKa = 4.02SSAVDD177 pKa = 4.85AITASDD183 pKa = 3.92FVFANAFSYY192 pKa = 9.5WQGQTLANASFSFFDD207 pKa = 6.33DD208 pKa = 2.89IMQALQVIQTAKK220 pKa = 10.58GSTDD224 pKa = 2.54ILFAVGEE231 pKa = 4.66TGWPTQGTSYY241 pKa = 11.35GDD243 pKa = 3.82AVPSTANAQTFFEE256 pKa = 4.25QGVCAMLGWGVDD268 pKa = 3.48VIYY271 pKa = 10.78FEE273 pKa = 6.09AFDD276 pKa = 4.5EE277 pKa = 4.45PWKK280 pKa = 11.0AGGSGTADD288 pKa = 3.04VEE290 pKa = 4.38PYY292 pKa = 10.59YY293 pKa = 11.08GAFDD297 pKa = 3.59DD298 pKa = 4.01TRR300 pKa = 11.84TLKK303 pKa = 10.72FPLTCNYY310 pKa = 8.71TGSSS314 pKa = 3.42

Molecular weight: 33.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E4TRM5|A0A1E4TRM5_PACTA Protein transport protein SEC22 OS=Pachysolen tannophilus NRRL Y-2460 OX=669874 GN=PACTADRAFT_18113 PE=3 SV=1
MM1 pKa = 7.52LPNIRR6 pKa = 11.84RR7 pKa = 11.84LFASFRR13 pKa = 11.84TEE15 pKa = 3.94EE16 pKa = 3.99EE17 pKa = 3.67EE18 pKa = 4.56KK19 pKa = 10.43IYY21 pKa = 10.73SRR23 pKa = 11.84KK24 pKa = 10.19AFYY27 pKa = 11.12NFLGFLASCVAFSFVAQQLARR48 pKa = 11.84QPRR51 pKa = 11.84EE52 pKa = 3.94LIHH55 pKa = 6.24AARR58 pKa = 11.84VNALLSAIQRR68 pKa = 11.84SS69 pKa = 3.62

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5643

2

5645

2707834

49

4960

479.7

54.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.17 ± 0.035

1.137 ± 0.011

5.987 ± 0.026

6.707 ± 0.034

4.643 ± 0.024

5.086 ± 0.031

1.881 ± 0.011

7.361 ± 0.028

7.751 ± 0.038

9.641 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.701 ± 0.009

7.431 ± 0.041

3.904 ± 0.022

3.81 ± 0.029

3.838 ± 0.016

9.119 ± 0.042

5.253 ± 0.025

5.213 ± 0.023

0.923 ± 0.008

3.446 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski