Euroglyphus maynei (Mayne s house dust mite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Acariformes; Sarcoptiformes; Astigmata; Psoroptidia; Analgoidea; Pyroglyphidae; Pyroglyphinae; Euroglyphus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15246 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3AXG1|A0A1Y3AXG1_EURMA Uncharacterized protein OS=Euroglyphus maynei OX=6958 GN=BLA29_013588 PE=4 SV=1
MM1 pKa = 7.57SSLKK5 pKa = 9.72RR6 pKa = 11.84TFDD9 pKa = 3.5QIDD12 pKa = 3.84PQPSDD17 pKa = 3.81DD18 pKa = 3.6QHH20 pKa = 6.6EE21 pKa = 4.55SSEE24 pKa = 4.25DD25 pKa = 3.14HH26 pKa = 6.79HH27 pKa = 6.53QQQQQSNDD35 pKa = 3.5DD36 pKa = 3.69CDD38 pKa = 5.39KK39 pKa = 11.07IIATEE44 pKa = 4.27MIDD47 pKa = 3.3HH48 pKa = 7.02QYY50 pKa = 9.63YY51 pKa = 9.01QHH53 pKa = 6.92NDD55 pKa = 3.04DD56 pKa = 5.64DD57 pKa = 6.51AIVDD61 pKa = 3.99DD62 pKa = 5.27NEE64 pKa = 4.17PAMKK68 pKa = 10.35LMKK71 pKa = 9.88IDD73 pKa = 4.62DD74 pKa = 4.19NNHH77 pKa = 5.37YY78 pKa = 10.55LIDD81 pKa = 4.13SSTSPLLLLSPQPPPEE97 pKa = 4.46SPPPEE102 pKa = 6.14LIDD105 pKa = 4.03KK106 pKa = 9.58CIQVDD111 pKa = 3.9TNAITEE117 pKa = 4.63DD118 pKa = 3.63IVTEE122 pKa = 4.18SAITTCYY129 pKa = 10.49DD130 pKa = 3.11INDD133 pKa = 3.98DD134 pKa = 3.77HH135 pKa = 6.78HH136 pKa = 7.7HH137 pKa = 6.75HH138 pKa = 6.12EE139 pKa = 4.5VIEE142 pKa = 4.2IEE144 pKa = 4.47LNSSSLNPEE153 pKa = 3.76QQQQQNDD160 pKa = 3.8SLTEE164 pKa = 3.84NSCQNDD170 pKa = 3.43QQQQLPEE177 pKa = 4.11DD178 pKa = 3.62QQ179 pKa = 4.03

Molecular weight: 20.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3BMI7|A0A1Y3BMI7_EURMA Calcium-independent phospholipase A2-gamma-like protein (Fragment) OS=Euroglyphus maynei OX=6958 GN=BLA29_009093 PE=4 SV=1
DDD2 pKa = 4.18QKKK5 pKa = 10.86LPLSRR10 pKa = 11.84FLGPQGGGKKK20 pKa = 9.89GGSGRR25 pKa = 11.84GGSRR29 pKa = 11.84GGRR32 pKa = 11.84AGGFGRR38 pKa = 11.84GGGGFGNRR46 pKa = 11.84GRR48 pKa = 11.84GNRR51 pKa = 11.84GGGFSSRR58 pKa = 11.84GGGSFGGRR66 pKa = 11.84GGGGRR71 pKa = 11.84GGSFGGNRR79 pKa = 11.84GRR81 pKa = 11.84GGGGFRR87 pKa = 11.84GGFRR91 pKa = 11.84GGRR94 pKa = 11.84RR95 pKa = 11.84

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15246

0

15246

3727433

8

3959

244.5

27.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.828 ± 0.021

1.876 ± 0.017

5.857 ± 0.018

5.849 ± 0.022

4.464 ± 0.019

4.696 ± 0.022

2.763 ± 0.013

7.496 ± 0.022

6.223 ± 0.022

8.947 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.676 ± 0.011

6.78 ± 0.022

4.31 ± 0.024

4.917 ± 0.024

5.12 ± 0.018

8.295 ± 0.031

5.495 ± 0.021

4.929 ± 0.017

1.007 ± 0.007

3.465 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski