Amino acid dipepetide frequency for Siphoviridae sp. cttb18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.957AlaAla: 1.957 ± 0.381
0.548AlaCys: 0.548 ± 0.217
3.367AlaAsp: 3.367 ± 0.427
4.306AlaGlu: 4.306 ± 0.563
2.505AlaPhe: 2.505 ± 0.424
4.385AlaGly: 4.385 ± 0.563
1.174AlaHis: 1.174 ± 0.238
5.168AlaIle: 5.168 ± 0.665
6.185AlaLys: 6.185 ± 0.926
6.342AlaLeu: 6.342 ± 0.681
2.036AlaMet: 2.036 ± 0.403
2.427AlaAsn: 2.427 ± 0.555
1.488AlaPro: 1.488 ± 0.321
2.74AlaGln: 2.74 ± 0.495
2.505AlaArg: 2.505 ± 0.507
2.975AlaSer: 2.975 ± 0.516
3.132AlaThr: 3.132 ± 0.559
5.011AlaVal: 5.011 ± 0.794
1.018AlaTrp: 1.018 ± 0.261
2.036AlaTyr: 2.036 ± 0.504
0.0AlaXaa: 0.0 ± 0.0
Cys
0.157CysAla: 0.157 ± 0.107
0.235CysCys: 0.235 ± 0.143
0.705CysAsp: 0.705 ± 0.263
0.548CysGlu: 0.548 ± 0.223
0.783CysPhe: 0.783 ± 0.252
1.018CysGly: 1.018 ± 0.277
0.47CysHis: 0.47 ± 0.179
0.861CysIle: 0.861 ± 0.252
0.783CysLys: 0.783 ± 0.276
0.861CysLeu: 0.861 ± 0.245
0.157CysMet: 0.157 ± 0.121
0.235CysAsn: 0.235 ± 0.119
0.47CysPro: 0.47 ± 0.188
0.861CysGln: 0.861 ± 0.271
0.626CysArg: 0.626 ± 0.232
0.705CysSer: 0.705 ± 0.257
0.548CysThr: 0.548 ± 0.241
0.626CysVal: 0.626 ± 0.206
0.078CysTrp: 0.078 ± 0.081
0.391CysTyr: 0.391 ± 0.16
0.0CysXaa: 0.0 ± 0.0
Asp
3.445AspAla: 3.445 ± 0.353
0.626AspCys: 0.626 ± 0.212
3.288AspAsp: 3.288 ± 0.658
4.619AspGlu: 4.619 ± 0.638
3.367AspPhe: 3.367 ± 0.482
3.758AspGly: 3.758 ± 0.558
0.861AspHis: 0.861 ± 0.259
4.698AspIle: 4.698 ± 0.707
4.541AspLys: 4.541 ± 0.538
5.637AspLeu: 5.637 ± 0.679
0.94AspMet: 0.94 ± 0.201
2.192AspAsn: 2.192 ± 0.541
2.349AspPro: 2.349 ± 0.413
2.114AspGln: 2.114 ± 0.447
1.879AspArg: 1.879 ± 0.344
2.271AspSer: 2.271 ± 0.327
2.505AspThr: 2.505 ± 0.447
3.367AspVal: 3.367 ± 0.479
1.096AspTrp: 1.096 ± 0.321
2.897AspTyr: 2.897 ± 0.519
0.0AspXaa: 0.0 ± 0.0
Glu
4.463GluAla: 4.463 ± 0.631
0.705GluCys: 0.705 ± 0.34
2.975GluAsp: 2.975 ± 0.493
6.812GluGlu: 6.812 ± 0.965
2.897GluPhe: 2.897 ± 0.575
3.915GluGly: 3.915 ± 0.634
1.331GluHis: 1.331 ± 0.377
4.933GluIle: 4.933 ± 0.712
8.299GluLys: 8.299 ± 0.879
6.733GluLeu: 6.733 ± 1.017
2.349GluMet: 2.349 ± 0.438
3.915GluAsn: 3.915 ± 0.446
1.801GluPro: 1.801 ± 0.292
3.445GluGln: 3.445 ± 0.581
3.132GluArg: 3.132 ± 0.53
2.819GluSer: 2.819 ± 0.394
2.819GluThr: 2.819 ± 0.423
4.933GluVal: 4.933 ± 0.569
1.566GluTrp: 1.566 ± 0.449
2.584GluTyr: 2.584 ± 0.412
0.0GluXaa: 0.0 ± 0.0
Phe
3.288PheAla: 3.288 ± 0.371
0.548PheCys: 0.548 ± 0.314
3.288PheAsp: 3.288 ± 0.482
2.897PheGlu: 2.897 ± 0.548
2.114PhePhe: 2.114 ± 0.405
2.819PheGly: 2.819 ± 0.44
1.488PheHis: 1.488 ± 0.378
2.819PheIle: 2.819 ± 0.419
3.915PheLys: 3.915 ± 0.619
4.385PheLeu: 4.385 ± 0.731
1.331PheMet: 1.331 ± 0.387
2.114PheAsn: 2.114 ± 0.435
1.723PhePro: 1.723 ± 0.421
1.957PheGln: 1.957 ± 0.389
1.409PheArg: 1.409 ± 0.347
2.427PheSer: 2.427 ± 0.44
2.975PheThr: 2.975 ± 0.476
3.054PheVal: 3.054 ± 0.517
0.548PheTrp: 0.548 ± 0.18
1.957PheTyr: 1.957 ± 0.443
0.0PheXaa: 0.0 ± 0.0
Gly
3.367GlyAla: 3.367 ± 0.503
0.705GlyCys: 0.705 ± 0.273
4.15GlyAsp: 4.15 ± 0.593
3.68GlyGlu: 3.68 ± 0.582
3.758GlyPhe: 3.758 ± 0.575
5.011GlyGly: 5.011 ± 0.753
0.783GlyHis: 0.783 ± 0.25
4.698GlyIle: 4.698 ± 0.566
5.794GlyLys: 5.794 ± 0.815
5.794GlyLeu: 5.794 ± 0.922
1.566GlyMet: 1.566 ± 0.384
2.662GlyAsn: 2.662 ± 0.434
0.94GlyPro: 0.94 ± 0.247
2.897GlyGln: 2.897 ± 0.722
2.427GlyArg: 2.427 ± 0.453
3.758GlySer: 3.758 ± 0.537
3.68GlyThr: 3.68 ± 0.493
4.463GlyVal: 4.463 ± 0.628
1.879GlyTrp: 1.879 ± 0.358
2.584GlyTyr: 2.584 ± 0.386
0.0GlyXaa: 0.0 ± 0.0
His
0.861HisAla: 0.861 ± 0.293
0.313HisCys: 0.313 ± 0.167
1.409HisAsp: 1.409 ± 0.402
1.331HisGlu: 1.331 ± 0.284
1.566HisPhe: 1.566 ± 0.4
1.253HisGly: 1.253 ± 0.315
0.548HisHis: 0.548 ± 0.211
0.783HisIle: 0.783 ± 0.256
1.566HisLys: 1.566 ± 0.305
1.801HisLeu: 1.801 ± 0.339
0.313HisMet: 0.313 ± 0.168
0.548HisAsn: 0.548 ± 0.197
0.626HisPro: 0.626 ± 0.194
0.626HisGln: 0.626 ± 0.202
1.096HisArg: 1.096 ± 0.246
0.783HisSer: 0.783 ± 0.285
1.018HisThr: 1.018 ± 0.258
0.548HisVal: 0.548 ± 0.23
0.705HisTrp: 0.705 ± 0.227
1.096HisTyr: 1.096 ± 0.276
0.0HisXaa: 0.0 ± 0.0
Ile
5.872IleAla: 5.872 ± 0.591
0.861IleCys: 0.861 ± 0.243
4.698IleAsp: 4.698 ± 0.615
4.933IleGlu: 4.933 ± 0.591
2.427IlePhe: 2.427 ± 0.451
4.463IleGly: 4.463 ± 0.683
1.018IleHis: 1.018 ± 0.264
4.385IleIle: 4.385 ± 0.564
5.637IleLys: 5.637 ± 0.609
4.463IleLeu: 4.463 ± 0.645
1.096IleMet: 1.096 ± 0.287
2.74IleAsn: 2.74 ± 0.451
3.054IlePro: 3.054 ± 0.523
2.74IleGln: 2.74 ± 0.503
3.993IleArg: 3.993 ± 0.647
4.228IleSer: 4.228 ± 0.593
4.15IleThr: 4.15 ± 0.452
4.385IleVal: 4.385 ± 0.528
1.488IleTrp: 1.488 ± 0.405
2.036IleTyr: 2.036 ± 0.438
0.0IleXaa: 0.0 ± 0.0
Lys
6.264LysAla: 6.264 ± 1.201
0.626LysCys: 0.626 ± 0.224
4.228LysAsp: 4.228 ± 0.601
7.203LysGlu: 7.203 ± 0.899
4.306LysPhe: 4.306 ± 0.557
5.481LysGly: 5.481 ± 0.771
1.253LysHis: 1.253 ± 0.374
7.047LysIle: 7.047 ± 0.679
6.577LysLys: 6.577 ± 0.827
7.047LysLeu: 7.047 ± 0.752
3.445LysMet: 3.445 ± 0.551
4.619LysAsn: 4.619 ± 0.631
3.68LysPro: 3.68 ± 0.537
3.523LysGln: 3.523 ± 0.508
4.619LysArg: 4.619 ± 0.582
5.402LysSer: 5.402 ± 0.543
4.854LysThr: 4.854 ± 0.532
6.264LysVal: 6.264 ± 0.68
0.94LysTrp: 0.94 ± 0.263
2.897LysTyr: 2.897 ± 0.438
0.0LysXaa: 0.0 ± 0.0
Leu
5.559LeuAla: 5.559 ± 0.585
0.861LeuCys: 0.861 ± 0.269
5.089LeuAsp: 5.089 ± 0.68
5.089LeuGlu: 5.089 ± 0.541
4.228LeuPhe: 4.228 ± 0.645
4.854LeuGly: 4.854 ± 0.652
1.566LeuHis: 1.566 ± 0.335
5.872LeuIle: 5.872 ± 0.627
8.065LeuLys: 8.065 ± 0.775
5.637LeuLeu: 5.637 ± 0.987
2.819LeuMet: 2.819 ± 0.464
4.385LeuAsn: 4.385 ± 0.54
4.15LeuPro: 4.15 ± 0.564
3.288LeuGln: 3.288 ± 0.605
3.68LeuArg: 3.68 ± 0.51
5.324LeuSer: 5.324 ± 0.605
6.264LeuThr: 6.264 ± 0.983
5.011LeuVal: 5.011 ± 0.559
1.566LeuTrp: 1.566 ± 0.304
2.74LeuTyr: 2.74 ± 0.491
0.0LeuXaa: 0.0 ± 0.0
Met
2.349MetAla: 2.349 ± 0.451
0.078MetCys: 0.078 ± 0.075
1.331MetAsp: 1.331 ± 0.288
1.644MetGlu: 1.644 ± 0.358
0.783MetPhe: 0.783 ± 0.248
1.644MetGly: 1.644 ± 0.262
0.313MetHis: 0.313 ± 0.173
2.036MetIle: 2.036 ± 0.393
2.584MetLys: 2.584 ± 0.557
2.349MetLeu: 2.349 ± 0.345
1.253MetMet: 1.253 ± 0.262
1.957MetAsn: 1.957 ± 0.397
1.096MetPro: 1.096 ± 0.292
1.488MetGln: 1.488 ± 0.408
1.409MetArg: 1.409 ± 0.333
1.488MetSer: 1.488 ± 0.303
1.723MetThr: 1.723 ± 0.418
1.409MetVal: 1.409 ± 0.331
0.235MetTrp: 0.235 ± 0.127
0.548MetTyr: 0.548 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
2.505AsnAla: 2.505 ± 0.496
0.548AsnCys: 0.548 ± 0.246
2.662AsnAsp: 2.662 ± 0.392
3.367AsnGlu: 3.367 ± 0.472
2.271AsnPhe: 2.271 ± 0.42
2.897AsnGly: 2.897 ± 0.467
0.94AsnHis: 0.94 ± 0.336
2.427AsnIle: 2.427 ± 0.365
4.15AsnLys: 4.15 ± 0.581
5.246AsnLeu: 5.246 ± 0.7
1.253AsnMet: 1.253 ± 0.348
2.349AsnAsn: 2.349 ± 0.441
2.505AsnPro: 2.505 ± 0.511
1.957AsnGln: 1.957 ± 0.351
1.801AsnArg: 1.801 ± 0.3
2.271AsnSer: 2.271 ± 0.435
2.036AsnThr: 2.036 ± 0.405
2.74AsnVal: 2.74 ± 0.362
1.096AsnTrp: 1.096 ± 0.306
2.427AsnTyr: 2.427 ± 0.487
0.0AsnXaa: 0.0 ± 0.0
Pro
1.253ProAla: 1.253 ± 0.278
0.235ProCys: 0.235 ± 0.138
1.879ProAsp: 1.879 ± 0.489
2.897ProGlu: 2.897 ± 0.43
1.879ProPhe: 1.879 ± 0.324
1.409ProGly: 1.409 ± 0.329
0.391ProHis: 0.391 ± 0.19
2.897ProIle: 2.897 ± 0.443
3.915ProLys: 3.915 ± 0.68
3.132ProLeu: 3.132 ± 0.429
1.096ProMet: 1.096 ± 0.283
2.662ProAsn: 2.662 ± 0.469
0.783ProPro: 0.783 ± 0.298
2.349ProGln: 2.349 ± 0.385
1.488ProArg: 1.488 ± 0.303
2.349ProSer: 2.349 ± 0.382
2.975ProThr: 2.975 ± 0.48
1.801ProVal: 1.801 ± 0.337
0.157ProTrp: 0.157 ± 0.111
1.096ProTyr: 1.096 ± 0.274
0.0ProXaa: 0.0 ± 0.0
Gln
2.897GlnAla: 2.897 ± 0.411
0.548GlnCys: 0.548 ± 0.216
1.879GlnAsp: 1.879 ± 0.331
3.837GlnGlu: 3.837 ± 0.562
1.957GlnPhe: 1.957 ± 0.363
3.837GlnGly: 3.837 ± 0.536
0.47GlnHis: 0.47 ± 0.155
2.662GlnIle: 2.662 ± 0.4
3.993GlnLys: 3.993 ± 0.629
3.523GlnLeu: 3.523 ± 0.609
1.096GlnMet: 1.096 ± 0.282
1.644GlnAsn: 1.644 ± 0.287
1.644GlnPro: 1.644 ± 0.257
1.801GlnGln: 1.801 ± 0.493
2.271GlnArg: 2.271 ± 0.384
2.427GlnSer: 2.427 ± 0.382
2.114GlnThr: 2.114 ± 0.325
2.662GlnVal: 2.662 ± 0.447
0.548GlnTrp: 0.548 ± 0.243
1.253GlnTyr: 1.253 ± 0.308
0.0GlnXaa: 0.0 ± 0.0
Arg
2.349ArgAla: 2.349 ± 0.408
0.626ArgCys: 0.626 ± 0.261
2.114ArgAsp: 2.114 ± 0.42
3.758ArgGlu: 3.758 ± 0.594
2.271ArgPhe: 2.271 ± 0.43
2.114ArgGly: 2.114 ± 0.493
0.861ArgHis: 0.861 ± 0.271
3.602ArgIle: 3.602 ± 0.527
3.602ArgLys: 3.602 ± 0.594
3.758ArgLeu: 3.758 ± 0.529
1.174ArgMet: 1.174 ± 0.344
2.819ArgAsn: 2.819 ± 0.456
1.409ArgPro: 1.409 ± 0.284
2.114ArgGln: 2.114 ± 0.392
1.801ArgArg: 1.801 ± 0.338
2.427ArgSer: 2.427 ± 0.392
1.566ArgThr: 1.566 ± 0.31
2.427ArgVal: 2.427 ± 0.406
0.861ArgTrp: 0.861 ± 0.221
1.801ArgTyr: 1.801 ± 0.356
0.0ArgXaa: 0.0 ± 0.0
Ser
3.054SerAla: 3.054 ± 0.413
0.861SerCys: 0.861 ± 0.251
2.897SerAsp: 2.897 ± 0.412
3.445SerGlu: 3.445 ± 0.54
2.271SerPhe: 2.271 ± 0.392
4.776SerGly: 4.776 ± 0.538
1.409SerHis: 1.409 ± 0.343
3.602SerIle: 3.602 ± 0.513
4.933SerLys: 4.933 ± 0.666
4.463SerLeu: 4.463 ± 0.591
0.94SerMet: 0.94 ± 0.247
3.21SerAsn: 3.21 ± 0.527
2.271SerPro: 2.271 ± 0.448
2.427SerGln: 2.427 ± 0.433
2.114SerArg: 2.114 ± 0.433
3.367SerSer: 3.367 ± 0.553
2.505SerThr: 2.505 ± 0.454
3.21SerVal: 3.21 ± 0.504
0.705SerTrp: 0.705 ± 0.258
1.723SerTyr: 1.723 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
3.915ThrAla: 3.915 ± 0.572
0.391ThrCys: 0.391 ± 0.172
3.915ThrAsp: 3.915 ± 0.458
3.602ThrGlu: 3.602 ± 0.64
2.662ThrPhe: 2.662 ± 0.498
3.993ThrGly: 3.993 ± 0.717
1.253ThrHis: 1.253 ± 0.291
3.054ThrIle: 3.054 ± 0.593
3.837ThrLys: 3.837 ± 0.491
5.716ThrLeu: 5.716 ± 1.116
1.801ThrMet: 1.801 ± 0.316
2.271ThrAsn: 2.271 ± 0.467
2.427ThrPro: 2.427 ± 0.576
1.879ThrGln: 1.879 ± 0.41
2.036ThrArg: 2.036 ± 0.43
2.975ThrSer: 2.975 ± 0.509
4.463ThrThr: 4.463 ± 0.708
2.271ThrVal: 2.271 ± 0.558
1.253ThrTrp: 1.253 ± 0.353
1.566ThrTyr: 1.566 ± 0.318
0.0ThrXaa: 0.0 ± 0.0
Val
4.385ValAla: 4.385 ± 0.592
1.018ValCys: 1.018 ± 0.265
3.288ValAsp: 3.288 ± 0.492
4.619ValGlu: 4.619 ± 0.568
1.957ValPhe: 1.957 ± 0.321
3.758ValGly: 3.758 ± 0.55
1.644ValHis: 1.644 ± 0.317
4.071ValIle: 4.071 ± 0.603
6.42ValLys: 6.42 ± 0.861
4.933ValLeu: 4.933 ± 0.663
1.879ValMet: 1.879 ± 0.447
2.662ValAsn: 2.662 ± 0.429
2.897ValPro: 2.897 ± 0.454
2.427ValGln: 2.427 ± 0.435
2.271ValArg: 2.271 ± 0.338
3.523ValSer: 3.523 ± 0.482
2.74ValThr: 2.74 ± 0.667
5.011ValVal: 5.011 ± 0.653
0.861ValTrp: 0.861 ± 0.228
1.879ValTyr: 1.879 ± 0.443
0.0ValXaa: 0.0 ± 0.0
Trp
0.861TrpAla: 0.861 ± 0.263
0.235TrpCys: 0.235 ± 0.126
0.94TrpAsp: 0.94 ± 0.238
1.018TrpGlu: 1.018 ± 0.268
1.644TrpPhe: 1.644 ± 0.397
0.94TrpGly: 0.94 ± 0.257
0.157TrpHis: 0.157 ± 0.108
1.096TrpIle: 1.096 ± 0.267
2.114TrpLys: 2.114 ± 0.325
1.174TrpLeu: 1.174 ± 0.374
0.548TrpMet: 0.548 ± 0.245
0.861TrpAsn: 0.861 ± 0.27
0.078TrpPro: 0.078 ± 0.077
0.626TrpGln: 0.626 ± 0.203
1.331TrpArg: 1.331 ± 0.283
1.018TrpSer: 1.018 ± 0.278
0.705TrpThr: 0.705 ± 0.253
1.253TrpVal: 1.253 ± 0.335
0.235TrpTrp: 0.235 ± 0.142
0.626TrpTyr: 0.626 ± 0.236
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.505TyrAla: 2.505 ± 0.461
0.548TyrCys: 0.548 ± 0.218
2.349TyrAsp: 2.349 ± 0.397
2.819TyrGlu: 2.819 ± 0.349
1.409TyrPhe: 1.409 ± 0.319
2.271TyrGly: 2.271 ± 0.377
0.861TyrHis: 0.861 ± 0.232
1.957TyrIle: 1.957 ± 0.39
3.445TyrLys: 3.445 ± 0.552
3.054TyrLeu: 3.054 ± 0.391
0.548TyrMet: 0.548 ± 0.193
1.174TyrAsn: 1.174 ± 0.327
1.253TyrPro: 1.253 ± 0.288
1.801TyrGln: 1.801 ± 0.482
1.566TyrArg: 1.566 ± 0.336
1.644TyrSer: 1.644 ± 0.351
2.505TyrThr: 2.505 ± 0.542
1.801TyrVal: 1.801 ± 0.356
0.626TyrTrp: 0.626 ± 0.225
1.644TyrTyr: 1.644 ± 0.337
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (12773 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski