Firmicutes bacterium CAG:94

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2410 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6ZL02|R6ZL02_9FIRM 4-hydroxy-tetrahydrodipicolinate reductase OS=Firmicutes bacterium CAG:94 OX=1262989 GN=dapB PE=3 SV=1
MM1 pKa = 7.02MRR3 pKa = 11.84IGSAIRR9 pKa = 11.84RR10 pKa = 11.84VLALALCVLLGCGAFAGCSQVDD32 pKa = 4.08DD33 pKa = 3.77IVSNVTAAGVFPVEE47 pKa = 4.4VNGVTISSRR56 pKa = 11.84PSRR59 pKa = 11.84VVVLSPSLADD69 pKa = 3.59VILALGCEE77 pKa = 4.17TQLAGASEE85 pKa = 4.96GCTQSALDD93 pKa = 3.84EE94 pKa = 4.44LEE96 pKa = 4.39KK97 pKa = 11.22VSADD101 pKa = 3.28SVEE104 pKa = 4.9AIQALQPDD112 pKa = 5.12LVLVDD117 pKa = 4.67PNSSGAQSALEE128 pKa = 3.99EE129 pKa = 4.39AGVTVLSVEE138 pKa = 4.24PATDD142 pKa = 3.21RR143 pKa = 11.84SDD145 pKa = 3.72FEE147 pKa = 4.45RR148 pKa = 11.84LYY150 pKa = 10.74SQVSSALLGGEE161 pKa = 3.87AGYY164 pKa = 11.03DD165 pKa = 3.35EE166 pKa = 6.1GIATAQDD173 pKa = 3.11IFITLDD179 pKa = 3.47NINRR183 pKa = 11.84IVPKK187 pKa = 9.9DD188 pKa = 3.78TITTACYY195 pKa = 10.13LYY197 pKa = 10.78DD198 pKa = 4.4LDD200 pKa = 4.67GSAVTGDD207 pKa = 3.71MFGSTIMSYY216 pKa = 11.07AGVTNVFEE224 pKa = 4.3SLEE227 pKa = 4.16GGTYY231 pKa = 10.52DD232 pKa = 4.13FQSLEE237 pKa = 3.78IANPNVIFCAPGLKK251 pKa = 9.83EE252 pKa = 4.01QIEE255 pKa = 4.4SDD257 pKa = 3.49SRR259 pKa = 11.84FADD262 pKa = 4.78FQAVQQGNVVEE273 pKa = 4.19MDD275 pKa = 3.39EE276 pKa = 6.51SLMEE280 pKa = 3.95WQGRR284 pKa = 11.84TIVEE288 pKa = 3.86TAYY291 pKa = 9.98EE292 pKa = 3.88ISAAAFPQLLEE303 pKa = 4.35SSSSVSDD310 pKa = 3.3PTQDD314 pKa = 3.04IEE316 pKa = 4.72SQVSSALEE324 pKa = 3.67AQAQYY329 pKa = 8.07EE330 pKa = 4.21TLEE333 pKa = 4.23QGDD336 pKa = 3.76QGDD339 pKa = 4.19SVQALQEE346 pKa = 4.05RR347 pKa = 11.84LTQLGYY353 pKa = 9.14LTDD356 pKa = 5.22PYY358 pKa = 11.45DD359 pKa = 3.54GMYY362 pKa = 10.99GSTTADD368 pKa = 3.28CVSRR372 pKa = 11.84FQTANGLEE380 pKa = 4.16ATGIADD386 pKa = 4.16PATQAALFASGALRR400 pKa = 11.84EE401 pKa = 4.87DD402 pKa = 3.91GTPLPEE408 pKa = 4.11EE409 pKa = 4.43SGEE412 pKa = 4.28SSTSQSSSSASEE424 pKa = 4.22SSSSASEE431 pKa = 4.1SSASEE436 pKa = 4.16SSSQASSASSGSEE449 pKa = 3.7SSSQASASQQ458 pKa = 3.0

Molecular weight: 47.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6YBB9|R6YBB9_9FIRM Oxidoreductase domain protein OS=Firmicutes bacterium CAG:94 OX=1262989 GN=BN815_02359 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 10.08DD3 pKa = 3.33YY4 pKa = 10.99ILGVDD9 pKa = 4.64LGGTNIKK16 pKa = 9.97AAAYY20 pKa = 10.2RR21 pKa = 11.84LGSYY25 pKa = 10.03EE26 pKa = 3.9KK27 pKa = 10.61VGEE30 pKa = 4.22KK31 pKa = 10.22RR32 pKa = 11.84LPTQVEE38 pKa = 4.61GGWEE42 pKa = 4.1HH43 pKa = 6.08VLGRR47 pKa = 11.84VLAALEE53 pKa = 3.89EE54 pKa = 4.53LLRR57 pKa = 11.84HH58 pKa = 5.32TPRR61 pKa = 11.84EE62 pKa = 3.79RR63 pKa = 11.84VLCVGMGVPGLPGGGEE79 pKa = 4.24PCPGHH84 pKa = 7.07LPGGVRR90 pKa = 11.84NRR92 pKa = 11.84HH93 pKa = 5.35RR94 pKa = 11.84PAGGRR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84DD102 pKa = 3.4AGGCRR107 pKa = 11.84LRR109 pKa = 11.84RR110 pKa = 11.84PPHH113 pKa = 5.58GGGRR117 pKa = 11.84PDD119 pKa = 3.3VSRR122 pKa = 11.84KK123 pKa = 9.85KK124 pKa = 10.67SFPFF128 pKa = 4.04

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2410

0

2410

675675

29

1557

280.4

30.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.319 ± 0.06

1.62 ± 0.024

5.051 ± 0.039

7.162 ± 0.058

3.972 ± 0.033

8.262 ± 0.057

1.938 ± 0.028

4.975 ± 0.046

4.794 ± 0.045

10.376 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.024

3.114 ± 0.034

4.539 ± 0.036

4.233 ± 0.031

5.436 ± 0.052

5.496 ± 0.046

5.19 ± 0.043

7.378 ± 0.046

1.12 ± 0.019

3.446 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski