Hubei sobemo-like virus 14

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEK1|A0A1L3KEK1_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 14 OX=1923199 PE=4 SV=1
MM1 pKa = 7.57VDD3 pKa = 3.25TMVDD7 pKa = 2.87RR8 pKa = 11.84LLFQEE13 pKa = 4.01WTANVLKK20 pKa = 10.8KK21 pKa = 10.36HH22 pKa = 6.03MDD24 pKa = 3.31LPLAMGWAPISGGYY38 pKa = 8.91RR39 pKa = 11.84QLLRR43 pKa = 11.84RR44 pKa = 11.84FPGKK48 pKa = 10.55ALMADD53 pKa = 3.34KK54 pKa = 11.09SSWDD58 pKa = 3.08WTAQSWVFEE67 pKa = 4.12ALLAVLFEE75 pKa = 5.14LGPDD79 pKa = 3.35NDD81 pKa = 3.19CWRR84 pKa = 11.84RR85 pKa = 11.84VARR88 pKa = 11.84GRR90 pKa = 11.84FRR92 pKa = 11.84ALFYY96 pKa = 10.22EE97 pKa = 4.36AVMQFSDD104 pKa = 3.7GTLIQQDD111 pKa = 3.79YY112 pKa = 8.31TGVMKK117 pKa = 10.57SGCFLTLAGNSVAQLLLTSLAKK139 pKa = 10.44LRR141 pKa = 11.84LGWSDD146 pKa = 3.94SGEE149 pKa = 3.93FWALGDD155 pKa = 3.84DD156 pKa = 4.2TIEE159 pKa = 4.33DD160 pKa = 3.68VVPDD164 pKa = 3.05VEE166 pKa = 5.19RR167 pKa = 11.84YY168 pKa = 9.24VKK170 pKa = 10.12EE171 pKa = 4.05LQSCGCVIKK180 pKa = 10.12EE181 pKa = 3.92YY182 pKa = 10.75SVSDD186 pKa = 3.34VLEE189 pKa = 3.95FVGFVFHH196 pKa = 7.43RR197 pKa = 11.84DD198 pKa = 3.64SIPTPSYY205 pKa = 9.14VDD207 pKa = 2.85KK208 pKa = 11.07HH209 pKa = 5.54VFQIEE214 pKa = 4.56HH215 pKa = 6.84IDD217 pKa = 3.54DD218 pKa = 3.66AVYY221 pKa = 10.89EE222 pKa = 4.33EE223 pKa = 4.68TLQQYY228 pKa = 11.09LLLHH232 pKa = 6.65LNSPLKK238 pKa = 10.53PYY240 pKa = 10.63LDD242 pKa = 3.57RR243 pKa = 11.84LAEE246 pKa = 4.18RR247 pKa = 11.84EE248 pKa = 3.89PAGTFMSDD256 pKa = 3.07QKK258 pKa = 11.2LKK260 pKa = 10.78RR261 pKa = 11.84IWLGG265 pKa = 3.25

Molecular weight: 30.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEK1|A0A1L3KEK1_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 14 OX=1923199 PE=4 SV=1
MM1 pKa = 7.14SQTVMSIVDD10 pKa = 3.97RR11 pKa = 11.84LSEE14 pKa = 4.14PQWFRR19 pKa = 11.84NVEE22 pKa = 4.18SMSNIALPWVLTTLMAFYY40 pKa = 10.72LFVPPILGGLRR51 pKa = 11.84RR52 pKa = 11.84VARR55 pKa = 11.84NLIWLVVSVRR65 pKa = 11.84MACKK69 pKa = 10.15VFWRR73 pKa = 11.84NTRR76 pKa = 11.84DD77 pKa = 3.3SFLGQPRR84 pKa = 11.84YY85 pKa = 10.15NPEE88 pKa = 3.52STMPGSPFVPVSKK101 pKa = 10.55LPKK104 pKa = 8.04FQCMVLGNSRR114 pKa = 11.84AGADD118 pKa = 3.49PVQVGVAFRR127 pKa = 11.84FEE129 pKa = 4.24DD130 pKa = 3.98WIVTAWHH137 pKa = 5.89VVEE140 pKa = 5.43CCSEE144 pKa = 3.59MWLSVEE150 pKa = 4.38GEE152 pKa = 4.3TVPISKK158 pKa = 10.45SSFDD162 pKa = 3.72HH163 pKa = 6.94LEE165 pKa = 3.63GDD167 pKa = 3.01IAYY170 pKa = 10.14APFSKK175 pKa = 10.77DD176 pKa = 2.89LGRR179 pKa = 11.84LGLSKK184 pKa = 10.66PKK186 pKa = 10.07IRR188 pKa = 11.84TAGTGRR194 pKa = 11.84FDD196 pKa = 3.29EE197 pKa = 5.97AYY199 pKa = 9.12VQVAGGSPLSQTNGALKK216 pKa = 9.88PSRR219 pKa = 11.84SFGYY223 pKa = 10.07VRR225 pKa = 11.84YY226 pKa = 8.11TGSTKK231 pKa = 10.31PGFSGAPYY239 pKa = 7.51YY240 pKa = 10.38QHH242 pKa = 6.31VFVYY246 pKa = 10.3GMHH249 pKa = 6.93LGGGTEE255 pKa = 4.01NLGLDD260 pKa = 3.46ATYY263 pKa = 10.64LHH265 pKa = 6.88SMLLATRR272 pKa = 11.84EE273 pKa = 4.23STPDD277 pKa = 3.1ILDD280 pKa = 3.0KK281 pKa = 11.65VMFGRR286 pKa = 11.84QPIAVRR292 pKa = 11.84SFGNKK297 pKa = 8.61YY298 pKa = 7.7MAKK301 pKa = 9.51TGSGYY306 pKa = 10.85KK307 pKa = 10.28NIMYY311 pKa = 9.92EE312 pKa = 4.44DD313 pKa = 4.03YY314 pKa = 11.49VEE316 pKa = 4.91LSNQGRR322 pKa = 11.84SWADD326 pKa = 3.01MVEE329 pKa = 4.38DD330 pKa = 4.08YY331 pKa = 11.09EE332 pKa = 5.44GEE334 pKa = 4.21ANLMYY339 pKa = 10.61DD340 pKa = 4.21DD341 pKa = 5.43EE342 pKa = 5.05EE343 pKa = 4.47PTLQEE348 pKa = 4.04NFNRR352 pKa = 11.84AALRR356 pKa = 11.84AARR359 pKa = 11.84VAASEE364 pKa = 4.28TSSQLSVISGAVSSRR379 pKa = 11.84CMRR382 pKa = 11.84KK383 pKa = 8.43DD384 pKa = 3.3TEE386 pKa = 4.08IQEE389 pKa = 4.34LRR391 pKa = 11.84DD392 pKa = 3.54QISQLRR398 pKa = 11.84KK399 pKa = 9.77LLINYY404 pKa = 8.83HH405 pKa = 5.13EE406 pKa = 4.63QILVQGKK413 pKa = 9.39IPTQTTEE420 pKa = 3.69TSTSEE425 pKa = 4.46LKK427 pKa = 10.52KK428 pKa = 9.46QQEE431 pKa = 4.18NLRR434 pKa = 11.84QKK436 pKa = 11.09SSGQLSKK443 pKa = 8.9TQKK446 pKa = 9.73KK447 pKa = 9.73RR448 pKa = 11.84LRR450 pKa = 11.84QRR452 pKa = 11.84LSKK455 pKa = 10.78NITGEE460 pKa = 3.48PSMRR464 pKa = 11.84ILKK467 pKa = 8.57EE468 pKa = 3.39HH469 pKa = 6.54SGILTQDD476 pKa = 3.11QPPAYY481 pKa = 8.42VTYY484 pKa = 10.21KK485 pKa = 9.58GTVRR489 pKa = 11.84PLEE492 pKa = 4.13KK493 pKa = 10.64SLDD496 pKa = 3.79GMGSGTQDD504 pKa = 3.03LRR506 pKa = 11.84SWLSSSVKK514 pKa = 10.23YY515 pKa = 10.38SAEE518 pKa = 3.79SGALKK523 pKa = 10.63GEE525 pKa = 4.28KK526 pKa = 9.9LLSSTQSRR534 pKa = 11.84SSLSRR539 pKa = 11.84RR540 pKa = 11.84RR541 pKa = 11.84TSRR544 pKa = 11.84QKK546 pKa = 11.18LEE548 pKa = 3.83

Molecular weight: 61.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

813

265

548

406.5

45.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.027 ± 0.415

1.107 ± 0.218

5.166 ± 1.497

5.904 ± 0.132

3.936 ± 0.731

7.257 ± 0.457

1.476 ± 0.223

3.444 ± 0.231

5.166 ± 0.346

10.701 ± 1.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.198 ± 0.097

2.583 ± 0.583

4.428 ± 0.355

5.043 ± 0.279

6.642 ± 0.533

9.594 ± 1.725

5.412 ± 0.684

7.134 ± 0.429

2.214 ± 0.642

3.567 ± 0.093
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski