Amino acid dipepetide frequency for Helicobacter phage Pt22899G

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.673AlaAla: 1.673 ± 0.529
0.558AlaCys: 0.558 ± 0.312
1.785AlaAsp: 1.785 ± 0.31
3.793AlaGlu: 3.793 ± 0.778
4.685AlaPhe: 4.685 ± 1.19
3.124AlaGly: 3.124 ± 0.862
0.781AlaHis: 0.781 ± 0.269
5.578AlaIle: 5.578 ± 0.985
9.482AlaLys: 9.482 ± 1.016
12.383AlaLeu: 12.383 ± 1.099
1.562AlaMet: 1.562 ± 0.498
5.801AlaAsn: 5.801 ± 0.835
1.673AlaPro: 1.673 ± 0.35
2.9AlaGln: 2.9 ± 0.457
3.124AlaArg: 3.124 ± 0.716
3.57AlaSer: 3.57 ± 0.503
2.454AlaThr: 2.454 ± 0.607
2.008AlaVal: 2.008 ± 0.575
0.335AlaTrp: 0.335 ± 0.192
2.343AlaTyr: 2.343 ± 0.49
0.0AlaXaa: 0.0 ± 0.0
Cys
0.335CysAla: 0.335 ± 0.245
0.0CysCys: 0.0 ± 0.0
0.446CysAsp: 0.446 ± 0.282
0.892CysGlu: 0.892 ± 0.376
0.669CysPhe: 0.669 ± 0.354
0.223CysGly: 0.223 ± 0.167
0.0CysHis: 0.0 ± 0.0
0.669CysIle: 0.669 ± 0.429
0.112CysLys: 0.112 ± 0.114
1.004CysLeu: 1.004 ± 0.444
0.0CysMet: 0.0 ± 0.0
0.335CysAsn: 0.335 ± 0.194
0.223CysPro: 0.223 ± 0.166
0.223CysGln: 0.223 ± 0.156
0.223CysArg: 0.223 ± 0.231
0.335CysSer: 0.335 ± 0.213
0.335CysThr: 0.335 ± 0.233
0.335CysVal: 0.335 ± 0.192
0.0CysTrp: 0.0 ± 0.0
0.112CysTyr: 0.112 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
2.566AspAla: 2.566 ± 0.436
0.335AspCys: 0.335 ± 0.266
1.004AspAsp: 1.004 ± 0.319
3.012AspGlu: 3.012 ± 0.59
4.016AspPhe: 4.016 ± 0.633
1.004AspGly: 1.004 ± 0.3
0.558AspHis: 0.558 ± 0.221
3.235AspIle: 3.235 ± 0.567
6.247AspLys: 6.247 ± 1.072
7.363AspLeu: 7.363 ± 1.006
1.116AspMet: 1.116 ± 0.383
4.797AspAsn: 4.797 ± 0.736
1.339AspPro: 1.339 ± 0.441
0.669AspGln: 0.669 ± 0.245
2.231AspArg: 2.231 ± 0.659
2.566AspSer: 2.566 ± 0.579
1.673AspThr: 1.673 ± 0.515
1.004AspVal: 1.004 ± 0.363
0.335AspTrp: 0.335 ± 0.186
2.789AspTyr: 2.789 ± 0.512
0.0AspXaa: 0.0 ± 0.0
Glu
6.917GluAla: 6.917 ± 0.897
0.223GluCys: 0.223 ± 0.16
2.454GluAsp: 2.454 ± 0.489
4.574GluGlu: 4.574 ± 1.184
4.128GluPhe: 4.128 ± 0.569
1.116GluGly: 1.116 ± 0.284
0.892GluHis: 0.892 ± 0.308
7.251GluIle: 7.251 ± 1.056
8.813GluLys: 8.813 ± 1.343
8.925GluLeu: 8.925 ± 1.489
1.785GluMet: 1.785 ± 0.482
7.251GluAsn: 7.251 ± 0.568
1.45GluPro: 1.45 ± 0.445
5.578GluGln: 5.578 ± 1.381
4.128GluArg: 4.128 ± 0.741
7.474GluSer: 7.474 ± 0.921
5.02GluThr: 5.02 ± 0.673
3.347GluVal: 3.347 ± 0.833
0.446GluTrp: 0.446 ± 0.194
1.896GluTyr: 1.896 ± 0.374
0.0GluXaa: 0.0 ± 0.0
Phe
1.227PheAla: 1.227 ± 0.372
0.558PheCys: 0.558 ± 0.314
2.454PheAsp: 2.454 ± 0.527
3.681PheGlu: 3.681 ± 0.631
2.9PhePhe: 2.9 ± 0.459
1.562PheGly: 1.562 ± 0.306
1.116PheHis: 1.116 ± 0.288
2.566PheIle: 2.566 ± 0.492
7.14PheLys: 7.14 ± 0.981
5.801PheLeu: 5.801 ± 0.839
0.558PheMet: 0.558 ± 0.32
3.012PheAsn: 3.012 ± 0.455
0.446PhePro: 0.446 ± 0.206
0.669PheGln: 0.669 ± 0.26
2.343PheArg: 2.343 ± 0.687
5.355PheSer: 5.355 ± 0.657
2.677PheThr: 2.677 ± 0.578
2.008PheVal: 2.008 ± 0.505
0.112PheTrp: 0.112 ± 0.133
1.673PheTyr: 1.673 ± 0.492
0.0PheXaa: 0.0 ± 0.0
Gly
3.235GlyAla: 3.235 ± 0.962
0.335GlyCys: 0.335 ± 0.222
1.785GlyAsp: 1.785 ± 0.434
2.9GlyGlu: 2.9 ± 0.605
2.343GlyPhe: 2.343 ± 0.58
3.012GlyGly: 3.012 ± 0.862
0.112GlyHis: 0.112 ± 0.117
2.343GlyIle: 2.343 ± 0.454
2.566GlyLys: 2.566 ± 0.569
6.136GlyLeu: 6.136 ± 0.681
1.339GlyMet: 1.339 ± 0.43
3.458GlyAsn: 3.458 ± 0.656
0.0GlyPro: 0.0 ± 0.0
0.781GlyGln: 0.781 ± 0.287
1.116GlyArg: 1.116 ± 0.323
2.9GlySer: 2.9 ± 0.728
1.004GlyThr: 1.004 ± 0.298
3.905GlyVal: 3.905 ± 0.912
0.0GlyTrp: 0.0 ± 0.0
1.673GlyTyr: 1.673 ± 0.29
0.0GlyXaa: 0.0 ± 0.0
His
1.116HisAla: 1.116 ± 0.365
0.223HisCys: 0.223 ± 0.228
1.004HisAsp: 1.004 ± 0.484
1.116HisGlu: 1.116 ± 0.32
0.558HisPhe: 0.558 ± 0.274
0.0HisGly: 0.0 ± 0.0
0.335HisHis: 0.335 ± 0.211
1.004HisIle: 1.004 ± 0.401
2.12HisLys: 2.12 ± 0.492
1.562HisLeu: 1.562 ± 0.55
0.223HisMet: 0.223 ± 0.158
1.339HisAsn: 1.339 ± 0.534
0.446HisPro: 0.446 ± 0.21
0.669HisGln: 0.669 ± 0.301
0.446HisArg: 0.446 ± 0.269
0.892HisSer: 0.892 ± 0.282
1.339HisThr: 1.339 ± 0.371
0.335HisVal: 0.335 ± 0.188
0.112HisTrp: 0.112 ± 0.08
0.669HisTyr: 0.669 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
5.355IleAla: 5.355 ± 0.594
0.558IleCys: 0.558 ± 0.271
4.239IleAsp: 4.239 ± 0.798
5.689IleGlu: 5.689 ± 0.725
1.896IlePhe: 1.896 ± 0.45
1.673IleGly: 1.673 ± 0.432
1.339IleHis: 1.339 ± 0.406
4.351IleIle: 4.351 ± 0.622
7.697IleLys: 7.697 ± 1.091
6.805IleLeu: 6.805 ± 0.803
1.227IleMet: 1.227 ± 0.272
4.797IleAsn: 4.797 ± 1.101
1.004IlePro: 1.004 ± 0.325
3.012IleGln: 3.012 ± 0.53
2.343IleArg: 2.343 ± 0.419
3.57IleSer: 3.57 ± 0.504
3.57IleThr: 3.57 ± 0.532
2.343IleVal: 2.343 ± 0.553
0.223IleTrp: 0.223 ± 0.163
2.454IleTyr: 2.454 ± 0.671
0.0IleXaa: 0.0 ± 0.0
Lys
9.259LysAla: 9.259 ± 1.089
0.223LysCys: 0.223 ± 0.19
6.693LysAsp: 6.693 ± 1.166
12.606LysGlu: 12.606 ± 1.548
2.789LysPhe: 2.789 ± 0.49
4.351LysGly: 4.351 ± 0.903
3.012LysHis: 3.012 ± 0.802
7.921LysIle: 7.921 ± 1.04
9.482LysLys: 9.482 ± 1.401
9.148LysLeu: 9.148 ± 1.207
1.673LysMet: 1.673 ± 0.487
9.705LysAsn: 9.705 ± 1.356
3.793LysPro: 3.793 ± 0.689
5.578LysGln: 5.578 ± 1.302
5.466LysArg: 5.466 ± 1.054
6.359LysSer: 6.359 ± 0.914
7.028LysThr: 7.028 ± 0.983
4.574LysVal: 4.574 ± 0.664
0.669LysTrp: 0.669 ± 0.289
2.343LysTyr: 2.343 ± 0.605
0.0LysXaa: 0.0 ± 0.0
Leu
7.251LeuAla: 7.251 ± 1.146
1.45LeuCys: 1.45 ± 0.557
5.02LeuAsp: 5.02 ± 0.698
12.16LeuGlu: 12.16 ± 1.562
3.793LeuPhe: 3.793 ± 0.845
6.359LeuGly: 6.359 ± 0.583
0.669LeuHis: 0.669 ± 0.29
5.243LeuIle: 5.243 ± 0.814
18.295LeuLys: 18.295 ± 1.737
7.809LeuLeu: 7.809 ± 1.085
2.231LeuMet: 2.231 ± 0.694
11.825LeuAsn: 11.825 ± 1.596
2.231LeuPro: 2.231 ± 0.47
4.574LeuGln: 4.574 ± 0.739
3.012LeuArg: 3.012 ± 0.577
6.247LeuSer: 6.247 ± 0.991
4.574LeuThr: 4.574 ± 0.82
4.239LeuVal: 4.239 ± 0.653
0.892LeuTrp: 0.892 ± 0.309
1.896LeuTyr: 1.896 ± 0.426
0.0LeuXaa: 0.0 ± 0.0
Met
0.558MetAla: 0.558 ± 0.249
0.0MetCys: 0.0 ± 0.0
1.785MetAsp: 1.785 ± 0.402
1.339MetGlu: 1.339 ± 0.369
1.227MetPhe: 1.227 ± 0.398
1.339MetGly: 1.339 ± 0.428
0.335MetHis: 0.335 ± 0.269
0.446MetIle: 0.446 ± 0.228
2.231MetLys: 2.231 ± 0.489
2.008MetLeu: 2.008 ± 0.379
0.112MetMet: 0.112 ± 0.106
2.008MetAsn: 2.008 ± 0.557
1.45MetPro: 1.45 ± 0.36
1.896MetGln: 1.896 ± 0.46
0.781MetArg: 0.781 ± 0.341
1.004MetSer: 1.004 ± 0.331
0.335MetThr: 0.335 ± 0.18
0.558MetVal: 0.558 ± 0.236
0.223MetTrp: 0.223 ± 0.234
0.335MetTyr: 0.335 ± 0.203
0.0MetXaa: 0.0 ± 0.0
Asn
9.594AsnAla: 9.594 ± 1.338
0.223AsnCys: 0.223 ± 0.167
3.235AsnAsp: 3.235 ± 0.556
8.032AsnGlu: 8.032 ± 1.243
3.905AsnPhe: 3.905 ± 0.797
2.454AsnGly: 2.454 ± 0.447
1.896AsnHis: 1.896 ± 0.487
3.681AsnIle: 3.681 ± 0.675
7.363AsnLys: 7.363 ± 0.739
8.144AsnLeu: 8.144 ± 1.151
1.785AsnMet: 1.785 ± 0.435
6.024AsnAsn: 6.024 ± 0.796
2.789AsnPro: 2.789 ± 0.867
6.136AsnGln: 6.136 ± 1.82
2.231AsnArg: 2.231 ± 0.374
4.128AsnSer: 4.128 ± 0.551
4.574AsnThr: 4.574 ± 0.743
2.12AsnVal: 2.12 ± 0.61
0.335AsnTrp: 0.335 ± 0.155
3.347AsnTyr: 3.347 ± 0.622
0.0AsnXaa: 0.0 ± 0.0
Pro
0.669ProAla: 0.669 ± 0.208
0.0ProCys: 0.0 ± 0.0
0.781ProAsp: 0.781 ± 0.264
1.004ProGlu: 1.004 ± 0.32
1.45ProPhe: 1.45 ± 0.447
0.335ProGly: 0.335 ± 0.297
0.223ProHis: 0.223 ± 0.161
1.785ProIle: 1.785 ± 0.366
3.793ProLys: 3.793 ± 0.55
3.347ProLeu: 3.347 ± 0.87
0.558ProMet: 0.558 ± 0.282
3.012ProAsn: 3.012 ± 0.547
0.335ProPro: 0.335 ± 0.192
0.558ProGln: 0.558 ± 0.22
0.558ProArg: 0.558 ± 0.285
2.789ProSer: 2.789 ± 0.459
1.896ProThr: 1.896 ± 0.627
0.781ProVal: 0.781 ± 0.43
0.0ProTrp: 0.0 ± 0.0
0.892ProTyr: 0.892 ± 0.359
0.0ProXaa: 0.0 ± 0.0
Gln
5.689GlnAla: 5.689 ± 0.906
0.335GlnCys: 0.335 ± 0.241
1.785GlnAsp: 1.785 ± 0.365
5.689GlnGlu: 5.689 ± 1.35
1.673GlnPhe: 1.673 ± 0.457
2.008GlnGly: 2.008 ± 0.54
0.669GlnHis: 0.669 ± 0.257
2.231GlnIle: 2.231 ± 0.494
6.359GlnLys: 6.359 ± 1.009
3.793GlnLeu: 3.793 ± 0.564
0.446GlnMet: 0.446 ± 0.266
3.458GlnAsn: 3.458 ± 0.517
0.558GlnPro: 0.558 ± 0.276
2.789GlnGln: 2.789 ± 0.897
1.116GlnArg: 1.116 ± 0.384
3.347GlnSer: 3.347 ± 0.375
2.231GlnThr: 2.231 ± 0.458
1.896GlnVal: 1.896 ± 0.489
0.335GlnTrp: 0.335 ± 0.165
0.781GlnTyr: 0.781 ± 0.322
0.0GlnXaa: 0.0 ± 0.0
Arg
3.124ArgAla: 3.124 ± 0.747
0.223ArgCys: 0.223 ± 0.167
2.343ArgAsp: 2.343 ± 0.402
3.458ArgGlu: 3.458 ± 0.797
2.343ArgPhe: 2.343 ± 0.574
1.227ArgGly: 1.227 ± 0.316
0.669ArgHis: 0.669 ± 0.285
2.12ArgIle: 2.12 ± 0.496
2.566ArgLys: 2.566 ± 0.736
5.02ArgLeu: 5.02 ± 0.774
0.892ArgMet: 0.892 ± 0.4
1.562ArgAsn: 1.562 ± 0.37
1.339ArgPro: 1.339 ± 0.491
1.45ArgGln: 1.45 ± 0.504
1.227ArgArg: 1.227 ± 0.391
2.789ArgSer: 2.789 ± 0.674
1.227ArgThr: 1.227 ± 0.474
1.896ArgVal: 1.896 ± 0.361
0.223ArgTrp: 0.223 ± 0.161
1.673ArgTyr: 1.673 ± 0.537
0.0ArgXaa: 0.0 ± 0.0
Ser
3.793SerAla: 3.793 ± 0.737
0.335SerCys: 0.335 ± 0.252
5.02SerAsp: 5.02 ± 0.715
5.689SerGlu: 5.689 ± 1.12
3.793SerPhe: 3.793 ± 0.722
3.905SerGly: 3.905 ± 0.993
0.781SerHis: 0.781 ± 0.239
3.793SerIle: 3.793 ± 0.481
5.466SerLys: 5.466 ± 0.643
7.586SerLeu: 7.586 ± 1.104
2.008SerMet: 2.008 ± 0.606
4.016SerAsn: 4.016 ± 0.527
1.004SerPro: 1.004 ± 0.322
2.566SerGln: 2.566 ± 0.475
2.008SerArg: 2.008 ± 0.461
2.566SerSer: 2.566 ± 0.756
1.785SerThr: 1.785 ± 0.369
5.132SerVal: 5.132 ± 0.795
0.558SerTrp: 0.558 ± 0.222
2.677SerTyr: 2.677 ± 0.476
0.0SerXaa: 0.0 ± 0.0
Thr
2.12ThrAla: 2.12 ± 0.567
0.335ThrCys: 0.335 ± 0.2
2.343ThrAsp: 2.343 ± 0.431
2.9ThrGlu: 2.9 ± 0.448
1.45ThrPhe: 1.45 ± 0.614
1.785ThrGly: 1.785 ± 0.572
1.004ThrHis: 1.004 ± 0.3
3.347ThrIle: 3.347 ± 0.524
4.574ThrLys: 4.574 ± 0.947
5.466ThrLeu: 5.466 ± 0.805
0.781ThrMet: 0.781 ± 0.224
3.681ThrAsn: 3.681 ± 0.566
2.9ThrPro: 2.9 ± 0.607
4.016ThrGln: 4.016 ± 0.682
1.896ThrArg: 1.896 ± 0.592
3.235ThrSer: 3.235 ± 0.845
3.681ThrThr: 3.681 ± 0.621
0.669ThrVal: 0.669 ± 0.373
0.446ThrTrp: 0.446 ± 0.249
1.339ThrTyr: 1.339 ± 0.431
0.0ThrXaa: 0.0 ± 0.0
Val
3.124ValAla: 3.124 ± 0.548
0.335ValCys: 0.335 ± 0.202
2.231ValAsp: 2.231 ± 0.558
2.12ValGlu: 2.12 ± 0.604
2.454ValPhe: 2.454 ± 0.347
3.793ValGly: 3.793 ± 0.691
0.223ValHis: 0.223 ± 0.181
3.458ValIle: 3.458 ± 0.616
4.239ValLys: 4.239 ± 0.668
3.905ValLeu: 3.905 ± 0.949
0.892ValMet: 0.892 ± 0.362
2.9ValAsn: 2.9 ± 0.713
0.558ValPro: 0.558 ± 0.248
0.669ValGln: 0.669 ± 0.286
1.562ValArg: 1.562 ± 0.352
2.9ValSer: 2.9 ± 0.709
1.116ValThr: 1.116 ± 0.342
2.231ValVal: 2.231 ± 0.761
0.446ValTrp: 0.446 ± 0.19
1.339ValTyr: 1.339 ± 0.604
0.0ValXaa: 0.0 ± 0.0
Trp
0.223TrpAla: 0.223 ± 0.183
0.0TrpCys: 0.0 ± 0.0
0.0TrpAsp: 0.0 ± 0.0
0.335TrpGlu: 0.335 ± 0.183
0.0TrpPhe: 0.0 ± 0.0
0.558TrpGly: 0.558 ± 0.281
0.112TrpHis: 0.112 ± 0.116
0.558TrpIle: 0.558 ± 0.218
0.669TrpLys: 0.669 ± 0.215
0.112TrpLeu: 0.112 ± 0.118
0.223TrpMet: 0.223 ± 0.18
0.446TrpAsn: 0.446 ± 0.273
0.0TrpPro: 0.0 ± 0.0
0.112TrpGln: 0.112 ± 0.08
0.335TrpArg: 0.335 ± 0.177
0.558TrpSer: 0.558 ± 0.294
0.446TrpThr: 0.446 ± 0.207
0.669TrpVal: 0.669 ± 0.243
0.0TrpTrp: 0.0 ± 0.0
0.335TrpTyr: 0.335 ± 0.224
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.785TyrAla: 1.785 ± 0.384
0.223TyrCys: 0.223 ± 0.156
1.45TyrAsp: 1.45 ± 0.332
2.677TyrGlu: 2.677 ± 0.639
1.785TyrPhe: 1.785 ± 0.542
1.116TyrGly: 1.116 ± 0.237
0.781TyrHis: 0.781 ± 0.278
2.566TyrIle: 2.566 ± 0.668
3.458TyrLys: 3.458 ± 0.578
3.57TyrLeu: 3.57 ± 0.629
0.446TyrMet: 0.446 ± 0.223
2.566TyrAsn: 2.566 ± 0.537
1.227TyrPro: 1.227 ± 0.37
2.343TyrGln: 2.343 ± 0.465
1.227TyrArg: 1.227 ± 0.32
1.896TyrSer: 1.896 ± 0.36
1.004TyrThr: 1.004 ± 0.385
0.446TyrVal: 0.446 ± 0.248
0.0TyrTrp: 0.0 ± 0.0
1.004TyrTyr: 1.004 ± 0.301
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 36 proteins (8965 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski