Amino acid dipepetide frequency for Opitutaceae bacterium EW11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.271AlaAla: 15.271 ± 0.138
1.143AlaCys: 1.143 ± 0.026
5.742AlaAsp: 5.742 ± 0.057
7.09AlaGlu: 7.09 ± 0.075
4.161AlaPhe: 4.161 ± 0.052
9.335AlaGly: 9.335 ± 0.086
2.134AlaHis: 2.134 ± 0.035
4.912AlaIle: 4.912 ± 0.05
4.261AlaLys: 4.261 ± 0.055
11.977AlaLeu: 11.977 ± 0.116
2.247AlaMet: 2.247 ± 0.038
3.08AlaAsn: 3.08 ± 0.056
5.825AlaPro: 5.825 ± 0.067
3.892AlaGln: 3.892 ± 0.058
7.886AlaArg: 7.886 ± 0.076
6.741AlaSer: 6.741 ± 0.069
5.897AlaThr: 5.897 ± 0.07
8.614AlaVal: 8.614 ± 0.075
1.779AlaTrp: 1.779 ± 0.038
2.581AlaTyr: 2.581 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
1.099CysAla: 1.099 ± 0.025
0.151CysCys: 0.151 ± 0.009
0.514CysAsp: 0.514 ± 0.018
0.49CysGlu: 0.49 ± 0.018
0.403CysPhe: 0.403 ± 0.015
0.933CysGly: 0.933 ± 0.026
0.255CysHis: 0.255 ± 0.018
0.416CysIle: 0.416 ± 0.013
0.175CysLys: 0.175 ± 0.01
0.965CysLeu: 0.965 ± 0.022
0.136CysMet: 0.136 ± 0.01
0.224CysAsn: 0.224 ± 0.013
0.494CysPro: 0.494 ± 0.02
0.255CysGln: 0.255 ± 0.013
0.765CysArg: 0.765 ± 0.021
0.587CysSer: 0.587 ± 0.022
0.44CysThr: 0.44 ± 0.015
0.729CysVal: 0.729 ± 0.023
0.164CysTrp: 0.164 ± 0.01
0.212CysTyr: 0.212 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
5.744AspAla: 5.744 ± 0.056
0.488AspCys: 0.488 ± 0.019
2.551AspAsp: 2.551 ± 0.035
3.06AspGlu: 3.06 ± 0.045
2.309AspPhe: 2.309 ± 0.041
4.819AspGly: 4.819 ± 0.067
1.037AspHis: 1.037 ± 0.027
1.961AspIle: 1.961 ± 0.034
1.403AspLys: 1.403 ± 0.031
5.319AspLeu: 5.319 ± 0.053
0.736AspMet: 0.736 ± 0.022
1.195AspAsn: 1.195 ± 0.026
3.32AspPro: 3.32 ± 0.042
1.629AspGln: 1.629 ± 0.034
3.981AspArg: 3.981 ± 0.051
2.665AspSer: 2.665 ± 0.041
2.46AspThr: 2.46 ± 0.042
3.833AspVal: 3.833 ± 0.046
0.976AspTrp: 0.976 ± 0.025
1.606AspTyr: 1.606 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
6.446GluAla: 6.446 ± 0.084
0.444GluCys: 0.444 ± 0.017
2.336GluAsp: 2.336 ± 0.034
3.149GluGlu: 3.149 ± 0.055
2.241GluPhe: 2.241 ± 0.038
3.619GluGly: 3.619 ± 0.049
1.255GluHis: 1.255 ± 0.029
3.309GluIle: 3.309 ± 0.048
2.773GluLys: 2.773 ± 0.043
6.191GluLeu: 6.191 ± 0.059
1.233GluMet: 1.233 ± 0.026
1.817GluAsn: 1.817 ± 0.03
2.797GluPro: 2.797 ± 0.038
2.271GluGln: 2.271 ± 0.043
4.722GluArg: 4.722 ± 0.064
3.127GluSer: 3.127 ± 0.04
3.363GluThr: 3.363 ± 0.046
3.839GluVal: 3.839 ± 0.055
0.838GluTrp: 0.838 ± 0.021
1.33GluTyr: 1.33 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.514PheAla: 4.514 ± 0.056
0.468PheCys: 0.468 ± 0.016
2.441PheAsp: 2.441 ± 0.039
2.158PheGlu: 2.158 ± 0.034
1.774PhePhe: 1.774 ± 0.036
3.519PheGly: 3.519 ± 0.053
0.821PheHis: 0.821 ± 0.023
1.474PheIle: 1.474 ± 0.033
1.106PheLys: 1.106 ± 0.024
3.702PheLeu: 3.702 ± 0.052
0.627PheMet: 0.627 ± 0.017
1.23PheAsn: 1.23 ± 0.025
1.924PhePro: 1.924 ± 0.032
1.261PheGln: 1.261 ± 0.026
2.81PheArg: 2.81 ± 0.043
2.949PheSer: 2.949 ± 0.04
2.281PheThr: 2.281 ± 0.033
3.177PheVal: 3.177 ± 0.037
0.717PheTrp: 0.717 ± 0.022
1.058PheTyr: 1.058 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
8.29GlyAla: 8.29 ± 0.077
0.877GlyCys: 0.877 ± 0.019
3.921GlyAsp: 3.921 ± 0.051
4.282GlyGlu: 4.282 ± 0.056
3.595GlyPhe: 3.595 ± 0.048
6.442GlyGly: 6.442 ± 0.087
1.637GlyHis: 1.637 ± 0.03
3.966GlyIle: 3.966 ± 0.049
3.344GlyLys: 3.344 ± 0.043
7.994GlyLeu: 7.994 ± 0.078
1.731GlyMet: 1.731 ± 0.031
2.32GlyAsn: 2.32 ± 0.04
3.295GlyPro: 3.295 ± 0.052
2.69GlyGln: 2.69 ± 0.044
5.801GlyArg: 5.801 ± 0.061
5.025GlySer: 5.025 ± 0.072
4.693GlyThr: 4.693 ± 0.067
6.148GlyVal: 6.148 ± 0.061
1.613GlyTrp: 1.613 ± 0.031
2.44GlyTyr: 2.44 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.249HisAla: 2.249 ± 0.039
0.222HisCys: 0.222 ± 0.012
1.091HisAsp: 1.091 ± 0.024
1.139HisGlu: 1.139 ± 0.023
0.95HisPhe: 0.95 ± 0.024
1.897HisGly: 1.897 ± 0.031
0.554HisHis: 0.554 ± 0.018
0.745HisIle: 0.745 ± 0.023
0.403HisLys: 0.403 ± 0.015
2.241HisLeu: 2.241 ± 0.039
0.301HisMet: 0.301 ± 0.012
0.489HisAsn: 0.489 ± 0.017
1.506HisPro: 1.506 ± 0.034
0.643HisGln: 0.643 ± 0.021
1.697HisArg: 1.697 ± 0.03
1.096HisSer: 1.096 ± 0.026
0.955HisThr: 0.955 ± 0.022
1.572HisVal: 1.572 ± 0.029
0.432HisTrp: 0.432 ± 0.013
0.609HisTyr: 0.609 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
5.488IleAla: 5.488 ± 0.054
0.462IleCys: 0.462 ± 0.015
2.783IleAsp: 2.783 ± 0.042
2.969IleGlu: 2.969 ± 0.045
1.445IlePhe: 1.445 ± 0.029
3.948IleGly: 3.948 ± 0.054
0.928IleHis: 0.928 ± 0.021
1.555IleIle: 1.555 ± 0.036
1.219IleLys: 1.219 ± 0.029
4.02IleLeu: 4.02 ± 0.055
0.567IleMet: 0.567 ± 0.017
1.266IleAsn: 1.266 ± 0.027
2.453IlePro: 2.453 ± 0.036
1.501IleGln: 1.501 ± 0.031
3.144IleArg: 3.144 ± 0.041
2.684IleSer: 2.684 ± 0.044
2.374IleThr: 2.374 ± 0.043
3.856IleVal: 3.856 ± 0.045
0.568IleTrp: 0.568 ± 0.015
1.075IleTyr: 1.075 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
3.694LysAla: 3.694 ± 0.059
0.204LysCys: 0.204 ± 0.009
1.769LysAsp: 1.769 ± 0.036
1.878LysGlu: 1.878 ± 0.04
1.036LysPhe: 1.036 ± 0.029
2.327LysGly: 2.327 ± 0.041
0.697LysHis: 0.697 ± 0.02
1.794LysIle: 1.794 ± 0.034
1.664LysLys: 1.664 ± 0.035
3.775LysLeu: 3.775 ± 0.049
0.78LysMet: 0.78 ± 0.022
1.203LysAsn: 1.203 ± 0.025
2.427LysPro: 2.427 ± 0.04
1.219LysGln: 1.219 ± 0.026
2.54LysArg: 2.54 ± 0.041
2.031LysSer: 2.031 ± 0.036
2.305LysThr: 2.305 ± 0.035
2.332LysVal: 2.332 ± 0.038
0.444LysTrp: 0.444 ± 0.017
0.838LysTyr: 0.838 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
12.646LeuAla: 12.646 ± 0.11
1.08LeuCys: 1.08 ± 0.024
5.175LeuAsp: 5.175 ± 0.058
5.649LeuGlu: 5.649 ± 0.067
3.925LeuPhe: 3.925 ± 0.058
8.279LeuGly: 8.279 ± 0.069
2.146LeuHis: 2.146 ± 0.032
4.169LeuIle: 4.169 ± 0.049
3.721LeuLys: 3.721 ± 0.045
10.555LeuLeu: 10.555 ± 0.108
1.639LeuMet: 1.639 ± 0.034
2.886LeuAsn: 2.886 ± 0.044
5.967LeuPro: 5.967 ± 0.059
3.436LeuGln: 3.436 ± 0.047
8.282LeuArg: 8.282 ± 0.084
6.761LeuSer: 6.761 ± 0.076
5.578LeuThr: 5.578 ± 0.07
7.708LeuVal: 7.708 ± 0.082
1.439LeuTrp: 1.439 ± 0.03
2.306LeuTyr: 2.306 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
1.926MetAla: 1.926 ± 0.036
0.134MetCys: 0.134 ± 0.009
0.875MetAsp: 0.875 ± 0.025
0.934MetGlu: 0.934 ± 0.023
0.539MetPhe: 0.539 ± 0.018
1.257MetGly: 1.257 ± 0.029
0.372MetHis: 0.372 ± 0.013
0.907MetIle: 0.907 ± 0.022
1.015MetLys: 1.015 ± 0.022
1.826MetLeu: 1.826 ± 0.029
0.381MetMet: 0.381 ± 0.015
0.811MetAsn: 0.811 ± 0.02
1.27MetPro: 1.27 ± 0.026
0.64MetGln: 0.64 ± 0.018
1.278MetArg: 1.278 ± 0.027
1.302MetSer: 1.302 ± 0.025
1.13MetThr: 1.13 ± 0.021
1.133MetVal: 1.133 ± 0.027
0.17MetTrp: 0.17 ± 0.01
0.306MetTyr: 0.306 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.116AsnAla: 3.116 ± 0.048
0.251AsnCys: 0.251 ± 0.014
1.468AsnAsp: 1.468 ± 0.029
1.404AsnGlu: 1.404 ± 0.028
1.023AsnPhe: 1.023 ± 0.025
2.602AsnGly: 2.602 ± 0.049
0.609AsnHis: 0.609 ± 0.018
1.091AsnIle: 1.091 ± 0.026
0.715AsnLys: 0.715 ± 0.022
3.092AsnLeu: 3.092 ± 0.054
0.405AsnMet: 0.405 ± 0.015
0.914AsnAsn: 0.914 ± 0.026
2.174AsnPro: 2.174 ± 0.036
0.968AsnGln: 0.968 ± 0.023
2.125AsnArg: 2.125 ± 0.035
1.607AsnSer: 1.607 ± 0.03
1.502AsnThr: 1.502 ± 0.038
2.16AsnVal: 2.16 ± 0.038
0.515AsnTrp: 0.515 ± 0.018
0.947AsnTyr: 0.947 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
7.048ProAla: 7.048 ± 0.074
0.38ProCys: 0.38 ± 0.017
3.295ProAsp: 3.295 ± 0.043
4.051ProGlu: 4.051 ± 0.053
2.068ProPhe: 2.068 ± 0.037
4.668ProGly: 4.668 ± 0.06
1.104ProHis: 1.104 ± 0.024
2.202ProIle: 2.202 ± 0.038
2.03ProLys: 2.03 ± 0.033
5.036ProLeu: 5.036 ± 0.058
1.112ProMet: 1.112 ± 0.025
1.608ProAsn: 1.608 ± 0.033
2.998ProPro: 2.998 ± 0.049
1.688ProGln: 1.688 ± 0.033
3.334ProArg: 3.334 ± 0.045
3.561ProSer: 3.561 ± 0.043
2.861ProThr: 2.861 ± 0.041
4.328ProVal: 4.328 ± 0.049
0.913ProTrp: 0.913 ± 0.021
1.236ProTyr: 1.236 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.711GlnAla: 3.711 ± 0.047
0.226GlnCys: 0.226 ± 0.011
1.326GlnAsp: 1.326 ± 0.026
1.716GlnGlu: 1.716 ± 0.033
1.246GlnPhe: 1.246 ± 0.025
2.19GlnGly: 2.19 ± 0.038
0.761GlnHis: 0.761 ± 0.02
1.746GlnIle: 1.746 ± 0.037
1.37GlnLys: 1.37 ± 0.03
3.906GlnLeu: 3.906 ± 0.055
0.684GlnMet: 0.684 ± 0.021
0.991GlnAsn: 0.991 ± 0.022
2.073GlnPro: 2.073 ± 0.035
1.412GlnGln: 1.412 ± 0.033
2.796GlnArg: 2.796 ± 0.042
1.941GlnSer: 1.941 ± 0.035
1.932GlnThr: 1.932 ± 0.034
2.461GlnVal: 2.461 ± 0.037
0.511GlnTrp: 0.511 ± 0.016
0.768GlnTyr: 0.768 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
7.567ArgAla: 7.567 ± 0.07
0.678ArgCys: 0.678 ± 0.022
3.814ArgAsp: 3.814 ± 0.044
4.71ArgGlu: 4.71 ± 0.056
3.321ArgPhe: 3.321 ± 0.046
4.949ArgGly: 4.949 ± 0.053
1.673ArgHis: 1.673 ± 0.037
3.866ArgIle: 3.866 ± 0.047
2.431ArgLys: 2.431 ± 0.043
8.187ArgLeu: 8.187 ± 0.078
1.553ArgMet: 1.553 ± 0.027
2.02ArgAsn: 2.02 ± 0.034
3.665ArgPro: 3.665 ± 0.048
2.675ArgGln: 2.675 ± 0.041
6.142ArgArg: 6.142 ± 0.076
4.413ArgSer: 4.413 ± 0.045
3.872ArgThr: 3.872 ± 0.045
5.401ArgVal: 5.401 ± 0.066
1.421ArgTrp: 1.421 ± 0.031
2.017ArgTyr: 2.017 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
7.094SerAla: 7.094 ± 0.074
0.518SerCys: 0.518 ± 0.019
3.1SerAsp: 3.1 ± 0.044
3.339SerGlu: 3.339 ± 0.04
2.475SerPhe: 2.475 ± 0.034
5.616SerGly: 5.616 ± 0.078
1.187SerHis: 1.187 ± 0.029
2.648SerIle: 2.648 ± 0.043
1.871SerLys: 1.871 ± 0.035
6.336SerLeu: 6.336 ± 0.066
1.193SerMet: 1.193 ± 0.025
1.576SerAsn: 1.576 ± 0.036
3.581SerPro: 3.581 ± 0.045
1.916SerGln: 1.916 ± 0.036
4.33SerArg: 4.33 ± 0.05
4.05SerSer: 4.05 ± 0.063
3.292SerThr: 3.292 ± 0.064
4.763SerVal: 4.763 ± 0.062
1.023SerTrp: 1.023 ± 0.025
1.616SerTyr: 1.616 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
6.182ThrAla: 6.182 ± 0.074
0.42ThrCys: 0.42 ± 0.016
2.702ThrAsp: 2.702 ± 0.044
2.749ThrGlu: 2.749 ± 0.044
2.218ThrPhe: 2.218 ± 0.034
4.852ThrGly: 4.852 ± 0.058
1.148ThrHis: 1.148 ± 0.027
2.536ThrIle: 2.536 ± 0.043
1.761ThrLys: 1.761 ± 0.032
5.826ThrLeu: 5.826 ± 0.064
0.908ThrMet: 0.908 ± 0.025
1.492ThrAsn: 1.492 ± 0.034
3.691ThrPro: 3.691 ± 0.044
1.685ThrGln: 1.685 ± 0.06
3.438ThrArg: 3.438 ± 0.042
3.245ThrSer: 3.245 ± 0.05
3.087ThrThr: 3.087 ± 0.07
4.421ThrVal: 4.421 ± 0.056
0.891ThrTrp: 0.891 ± 0.023
1.45ThrTyr: 1.45 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
8.566ValAla: 8.566 ± 0.079
0.812ValCys: 0.812 ± 0.024
3.873ValAsp: 3.873 ± 0.041
4.213ValGlu: 4.213 ± 0.05
3.39ValPhe: 3.39 ± 0.046
5.702ValGly: 5.702 ± 0.064
1.511ValHis: 1.511 ± 0.033
3.325ValIle: 3.325 ± 0.042
2.441ValLys: 2.441 ± 0.045
7.806ValLeu: 7.806 ± 0.08
1.248ValMet: 1.248 ± 0.026
2.171ValAsn: 2.171 ± 0.04
4.004ValPro: 4.004 ± 0.053
2.378ValGln: 2.378 ± 0.033
5.648ValArg: 5.648 ± 0.062
4.991ValSer: 4.991 ± 0.056
4.279ValThr: 4.279 ± 0.056
6.358ValVal: 6.358 ± 0.07
1.146ValTrp: 1.146 ± 0.025
1.836ValTyr: 1.836 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.415TrpAla: 1.415 ± 0.03
0.19TrpCys: 0.19 ± 0.01
0.805TrpAsp: 0.805 ± 0.022
0.801TrpGlu: 0.801 ± 0.02
0.68TrpPhe: 0.68 ± 0.02
1.07TrpGly: 1.07 ± 0.028
0.379TrpHis: 0.379 ± 0.015
0.865TrpIle: 0.865 ± 0.025
0.648TrpLys: 0.648 ± 0.018
1.894TrpLeu: 1.894 ± 0.034
0.404TrpMet: 0.404 ± 0.014
0.588TrpAsn: 0.588 ± 0.018
0.766TrpPro: 0.766 ± 0.024
0.653TrpGln: 0.653 ± 0.02
1.343TrpArg: 1.343 ± 0.026
1.097TrpSer: 1.097 ± 0.026
1.0TrpThr: 1.0 ± 0.025
1.035TrpVal: 1.035 ± 0.027
0.353TrpTrp: 0.353 ± 0.015
0.4TrpTyr: 0.4 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.502TyrAla: 2.502 ± 0.038
0.26TyrCys: 0.26 ± 0.012
1.512TyrAsp: 1.512 ± 0.032
1.319TyrGlu: 1.319 ± 0.029
1.211TyrPhe: 1.211 ± 0.025
2.08TyrGly: 2.08 ± 0.039
0.583TyrHis: 0.583 ± 0.018
0.821TyrIle: 0.821 ± 0.021
0.605TyrLys: 0.605 ± 0.02
2.7TyrLeu: 2.7 ± 0.042
0.321TyrMet: 0.321 ± 0.015
0.807TyrAsn: 0.807 ± 0.027
1.289TyrPro: 1.289 ± 0.024
0.951TyrGln: 0.951 ± 0.025
2.315TyrArg: 2.315 ± 0.041
1.652TyrSer: 1.652 ± 0.033
1.399TyrThr: 1.399 ± 0.035
1.832TyrVal: 1.832 ± 0.033
0.473TyrTrp: 0.473 ± 0.015
0.819TyrTyr: 0.819 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4994 proteins (1872195 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski