Actinoplanes teichomyceticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Actinoplanes

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7203 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A561WJN4|A0A561WJN4_ACTTI Glycosyl transferase family 2 OS=Actinoplanes teichomyceticus OX=1867 GN=FHX34_102652 PE=4 SV=1
MM1 pKa = 7.18TFEE4 pKa = 4.44NVGPASDD11 pKa = 3.44IAKK14 pKa = 8.85GTVLQVEE21 pKa = 4.16IDD23 pKa = 3.7GVEE26 pKa = 4.0IAVVHH31 pKa = 6.9ADD33 pKa = 3.4DD34 pKa = 4.57DD35 pKa = 3.83NYY37 pKa = 11.16YY38 pKa = 10.96AVRR41 pKa = 11.84DD42 pKa = 4.09EE43 pKa = 4.7CSHH46 pKa = 6.77ASVALSEE53 pKa = 4.65GEE55 pKa = 3.95VDD57 pKa = 4.97GCTLEE62 pKa = 4.28CWLHH66 pKa = 6.03GSRR69 pKa = 11.84FDD71 pKa = 4.61LRR73 pKa = 11.84TGEE76 pKa = 4.11PSGPPAIDD84 pKa = 3.3PVAVYY89 pKa = 9.48PVEE92 pKa = 4.29IRR94 pKa = 11.84DD95 pKa = 3.22GDD97 pKa = 3.87IYY99 pKa = 11.59VSTTPSNGVEE109 pKa = 4.05PP110 pKa = 4.39

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A561WLD8|A0A561WLD8_ACTTI DNA-binding SARP family transcriptional activator OS=Actinoplanes teichomyceticus OX=1867 GN=FHX34_1021242 PE=3 SV=1
MM1 pKa = 7.69ASHH4 pKa = 7.41PPALPPGEE12 pKa = 4.15RR13 pKa = 11.84PPRR16 pKa = 11.84HH17 pKa = 5.82SPAPRR22 pKa = 11.84SPHH25 pKa = 6.06HH26 pKa = 7.0PSRR29 pKa = 11.84PPWTRR34 pKa = 11.84AEE36 pKa = 4.08PPARR40 pKa = 11.84TRR42 pKa = 11.84RR43 pKa = 11.84PRR45 pKa = 11.84RR46 pKa = 11.84PRR48 pKa = 11.84PPRR51 pKa = 11.84APAARR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84HH59 pKa = 5.56RR60 pKa = 11.84SRR62 pKa = 11.84RR63 pKa = 11.84SPGPPPRR70 pKa = 11.84PPVPHH75 pKa = 6.39RR76 pKa = 11.84PSRR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84SPRR89 pKa = 11.84SPQPPRR95 pKa = 11.84RR96 pKa = 11.84TSPRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84SRR105 pKa = 11.84ATGASRR111 pKa = 11.84LPSPARR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84AQVPRR124 pKa = 11.84GPRR127 pKa = 11.84PRR129 pKa = 11.84SPDD132 pKa = 3.04RR133 pKa = 11.84AKK135 pKa = 10.84ARR137 pKa = 11.84PRR139 pKa = 11.84PAAPCGAGPAGRR151 pKa = 11.84HH152 pKa = 4.16RR153 pKa = 11.84RR154 pKa = 11.84RR155 pKa = 11.84LRR157 pKa = 11.84VLRR160 pKa = 11.84RR161 pKa = 11.84PRR163 pKa = 11.84RR164 pKa = 11.84RR165 pKa = 11.84RR166 pKa = 11.84LRR168 pKa = 11.84VLRR171 pKa = 11.84RR172 pKa = 11.84PRR174 pKa = 11.84RR175 pKa = 11.84HH176 pKa = 5.73RR177 pKa = 11.84PGVLGRR183 pKa = 11.84PRR185 pKa = 11.84RR186 pKa = 11.84RR187 pKa = 11.84RR188 pKa = 11.84VGLSPPGLPSPSHH201 pKa = 5.95GSRR204 pKa = 11.84RR205 pKa = 11.84RR206 pKa = 11.84RR207 pKa = 11.84AVRR210 pKa = 11.84IPRR213 pKa = 11.84MPHH216 pKa = 5.16AAARR220 pKa = 11.84MPHH223 pKa = 6.2AAARR227 pKa = 11.84MPHH230 pKa = 6.2AAARR234 pKa = 11.84MPHH237 pKa = 6.2AAARR241 pKa = 11.84MPHH244 pKa = 6.2AAARR248 pKa = 11.84MPHH251 pKa = 6.2AAARR255 pKa = 11.84MPHH258 pKa = 6.2AAARR262 pKa = 11.84MPHH265 pKa = 6.46AAACGRR271 pKa = 11.84RR272 pKa = 11.84PRR274 pKa = 11.84RR275 pKa = 11.84KK276 pKa = 8.91PPQVRR281 pKa = 11.84PLPRR285 pKa = 11.84GRR287 pKa = 11.84NPGSRR292 pKa = 11.84ALSRR296 pKa = 11.84QARR299 pKa = 11.84PGPLRR304 pKa = 11.84GLRR307 pKa = 11.84RR308 pKa = 11.84RR309 pKa = 11.84RR310 pKa = 11.84SRR312 pKa = 11.84PSRR315 pKa = 11.84AAQPGQPSRR324 pKa = 11.84QPGRR328 pKa = 11.84LRR330 pKa = 11.84LLSRR334 pKa = 11.84QPGRR338 pKa = 11.84QRR340 pKa = 11.84RR341 pKa = 11.84GPGRR345 pKa = 11.84RR346 pKa = 11.84SHH348 pKa = 6.91RR349 pKa = 11.84SGRR352 pKa = 11.84LRR354 pKa = 11.84RR355 pKa = 11.84EE356 pKa = 3.77PGRR359 pKa = 11.84VRR361 pKa = 11.84RR362 pKa = 11.84QRR364 pKa = 11.84QRR366 pKa = 11.84RR367 pKa = 11.84GRR369 pKa = 11.84RR370 pKa = 11.84SPLRR374 pKa = 11.84SQARR378 pKa = 11.84RR379 pKa = 11.84TCRR382 pKa = 11.84GRR384 pKa = 11.84PWRR387 pKa = 11.84CRR389 pKa = 11.84PRR391 pKa = 11.84WLPAGRR397 pKa = 11.84RR398 pKa = 11.84RR399 pKa = 11.84GWTRR403 pKa = 11.84VQPSPPIRR411 pKa = 11.84PKK413 pKa = 9.95SAARR417 pKa = 11.84GAVLPGPGAGPVAEE431 pKa = 4.27PFGARR436 pKa = 11.84TALPAPSPP444 pKa = 3.76

Molecular weight: 50.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7203

0

7203

2375611

26

6666

329.8

35.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.587 ± 0.045

0.757 ± 0.007

5.9 ± 0.023

4.906 ± 0.028

2.649 ± 0.016

9.475 ± 0.033

2.193 ± 0.014

3.357 ± 0.018

1.644 ± 0.022

10.236 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.67 ± 0.012

1.775 ± 0.02

6.433 ± 0.034

2.712 ± 0.014

8.588 ± 0.034

4.775 ± 0.02

6.136 ± 0.034

8.593 ± 0.029

1.559 ± 0.014

2.054 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski