bacterium NHP-B

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S1M5J6|A0A4S1M5J6_9BACT HNH endonuclease (Fragment) OS=bacterium NHP-B OX=2491344 GN=EIL50_05565 PE=4 SV=1
QQ1 pKa = 6.91WNAPCSEE8 pKa = 4.32LSMGMSNDD16 pKa = 3.0YY17 pKa = 10.1TIAVEE22 pKa = 4.23EE23 pKa = 4.5GATFVRR29 pKa = 11.84IGSSLVGNEE38 pKa = 4.01TGGGQSS44 pKa = 3.18

Molecular weight: 4.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S1M790|A0A4S1M790_9BACT Uncharacterized protein OS=bacterium NHP-B OX=2491344 GN=EIL50_04360 PE=4 SV=1
MM1 pKa = 7.47PRR3 pKa = 11.84ASFLSMSSLAHH14 pKa = 5.61VSTWMQRR21 pKa = 11.84AVSILLPFRR30 pKa = 11.84CPLCRR35 pKa = 11.84TPLDD39 pKa = 3.54NRR41 pKa = 11.84GLCGEE46 pKa = 4.82CWRR49 pKa = 11.84KK50 pKa = 8.29ITFLSGPLCQTCGTPLPFAVSQTFTCGSCLAHH82 pKa = 6.49PPPFQEE88 pKa = 4.03LRR90 pKa = 11.84SAFLYY95 pKa = 10.46DD96 pKa = 3.3EE97 pKa = 4.76HH98 pKa = 9.18SRR100 pKa = 11.84ALLLHH105 pKa = 6.27LKK107 pKa = 9.7NRR109 pKa = 11.84QGLFLMPLLAHH120 pKa = 7.03LLMRR124 pKa = 11.84LMPSLTGPFDD134 pKa = 3.34WVVPVPLHH142 pKa = 6.22RR143 pKa = 11.84RR144 pKa = 11.84RR145 pKa = 11.84LFKK148 pKa = 10.8RR149 pKa = 11.84GFNQAAPLAQQLAQRR164 pKa = 11.84YY165 pKa = 8.39ALTYY169 pKa = 10.85VPFCLKK175 pKa = 10.21RR176 pKa = 11.84HH177 pKa = 5.58RR178 pKa = 11.84HH179 pKa = 4.85TPSQGGLNIRR189 pKa = 11.84QRR191 pKa = 11.84RR192 pKa = 11.84DD193 pKa = 3.07NVRR196 pKa = 11.84TAFSVPNPWQQKK208 pKa = 6.48VTGKK212 pKa = 10.68CIVLVDD218 pKa = 3.88DD219 pKa = 4.99VYY221 pKa = 9.51TTGATLSACAKK232 pKa = 9.9GLLAAGAKK240 pKa = 9.29RR241 pKa = 11.84VSALTLARR249 pKa = 11.84TPLSKK254 pKa = 11.06NPLGG258 pKa = 3.91

Molecular weight: 28.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1115

0

1115

343533

23

1396

308.1

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.61 ± 0.073

1.338 ± 0.025

5.126 ± 0.052

5.487 ± 0.067

4.594 ± 0.065

6.959 ± 0.068

3.429 ± 0.06

4.702 ± 0.056

5.537 ± 0.079

10.906 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.029

2.656 ± 0.042

5.236 ± 0.062

4.203 ± 0.046

5.425 ± 0.053

6.515 ± 0.066

5.659 ± 0.052

6.902 ± 0.068

1.377 ± 0.03

2.66 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski