Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) (Frog chytrid fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Chytridiomycetes; Rhizophydiales; Rhizophydiales incertae sedis; Batrachochytrium; Batrachochytrium dendrobatidis

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8610 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4PF81|F4PF81_BATDJ Sulfhydryl oxidase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=BATDEDRAFT_15048 PE=4 SV=1
MM1 pKa = 7.62HH2 pKa = 7.64LPLQLQPYY10 pKa = 9.57ADD12 pKa = 3.62VLAEE16 pKa = 4.1TKK18 pKa = 10.69KK19 pKa = 10.56PGFIINGKK27 pKa = 8.9PEE29 pKa = 4.29STTPLQSKK37 pKa = 10.11FSGRR41 pKa = 11.84PYY43 pKa = 9.66WLKK46 pKa = 11.03ADD48 pKa = 5.05AYY50 pKa = 9.74PITQNGQPLRR60 pKa = 11.84LLAQINFSEE69 pKa = 4.38MEE71 pKa = 4.09EE72 pKa = 4.31TIPDD76 pKa = 3.88YY77 pKa = 9.13PTEE80 pKa = 4.95GILQFFVADD89 pKa = 3.85DD90 pKa = 3.91DD91 pKa = 5.32VYY93 pKa = 11.71GLNFDD98 pKa = 5.46DD99 pKa = 5.47GTNQDD104 pKa = 3.49TFRR107 pKa = 11.84VVYY110 pKa = 10.35HH111 pKa = 5.93EE112 pKa = 4.25TVEE115 pKa = 4.06MDD117 pKa = 3.5PSKK120 pKa = 10.89WMTDD124 pKa = 3.55FPEE127 pKa = 4.74FGNEE131 pKa = 3.48NYY133 pKa = 10.1FPVEE137 pKa = 4.1KK138 pKa = 10.16EE139 pKa = 3.9CAISFDD145 pKa = 3.56VSEE148 pKa = 5.45EE149 pKa = 3.96IMSSSDD155 pKa = 3.01YY156 pKa = 10.45RR157 pKa = 11.84FNIITEE163 pKa = 4.02VDD165 pKa = 3.29QLLEE169 pKa = 4.36SGTDD173 pKa = 3.1EE174 pKa = 5.81DD175 pKa = 4.8YY176 pKa = 11.25AAHH179 pKa = 7.08DD180 pKa = 4.91EE181 pKa = 4.69IMDD184 pKa = 4.6AYY186 pKa = 10.8HH187 pKa = 7.23EE188 pKa = 4.33IASGQGCKK196 pKa = 10.34LGGYY200 pKa = 9.26SFFTQEE206 pKa = 4.29DD207 pKa = 3.42PRR209 pKa = 11.84AYY211 pKa = 10.38GDD213 pKa = 3.99YY214 pKa = 9.55LTHH217 pKa = 7.22DD218 pKa = 4.04TLLLQIDD225 pKa = 4.56SDD227 pKa = 4.4DD228 pKa = 4.84DD229 pKa = 3.97MNIMWGDD236 pKa = 3.31TGVANFFISSEE247 pKa = 3.99DD248 pKa = 3.28LKK250 pKa = 11.48NRR252 pKa = 11.84DD253 pKa = 3.39FSKK256 pKa = 11.02VLYY259 pKa = 10.64NWDD262 pKa = 3.64CYY264 pKa = 11.2

Molecular weight: 30.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4P5P6|F4P5P6_BATDJ 40S ribosomal protein S4 OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=BATDEDRAFT_35382 PE=3 SV=1
MM1 pKa = 8.06DD2 pKa = 4.77SADD5 pKa = 3.88TACSSVKK12 pKa = 10.0ICLVPLAALNLSKK25 pKa = 10.85QITEE29 pKa = 3.97EE30 pKa = 4.1LLCQFFSFIGPIVSISLQPCEE51 pKa = 4.73ANPLVQEE58 pKa = 4.57SLVQFQDD65 pKa = 3.72SEE67 pKa = 4.37DD68 pKa = 3.58AALALHH74 pKa = 6.18LTGTVLADD82 pKa = 3.22KK83 pKa = 10.68ALFITPPSAKK93 pKa = 8.8IQSFNSHH100 pKa = 6.41GYY102 pKa = 10.85LNIPPPNPTAPGVLVPGSMAGSTIVPYY129 pKa = 10.71GQTATIAGQDD139 pKa = 3.53SVDD142 pKa = 3.28RR143 pKa = 11.84TIYY146 pKa = 9.5TGNIHH151 pKa = 6.82SGLSQQEE158 pKa = 3.86VSMLFSSCGDD168 pKa = 3.29VTQVKK173 pKa = 8.66MAGDD177 pKa = 3.83ATHH180 pKa = 5.59STRR183 pKa = 11.84YY184 pKa = 10.43AFIEE188 pKa = 4.35FATSEE193 pKa = 4.34SAAMALNLHH202 pKa = 5.72GMMVAGRR209 pKa = 11.84AIKK212 pKa = 10.44VNRR215 pKa = 11.84SKK217 pKa = 11.07HH218 pKa = 5.64SIGRR222 pKa = 11.84PIGMYY227 pKa = 9.95PGMFRR232 pKa = 11.84PDD234 pKa = 2.78TDD236 pKa = 3.25MVMKK240 pKa = 10.35QAMQAQMRR248 pKa = 11.84MGHH251 pKa = 5.8SFSGDD256 pKa = 2.81ARR258 pKa = 11.84SAGKK262 pKa = 10.03FPIIPGTPGMMAIAAAANVPPGTNVMPPMTSYY294 pKa = 11.05LSAMHH299 pKa = 6.99APNPLANINAAATSTAARR317 pKa = 11.84TSQEE321 pKa = 3.5KK322 pKa = 10.09SSRR325 pKa = 11.84GRR327 pKa = 11.84TFSRR331 pKa = 11.84EE332 pKa = 3.7RR333 pKa = 11.84GRR335 pKa = 11.84DD336 pKa = 3.05RR337 pKa = 11.84HH338 pKa = 5.58EE339 pKa = 4.91SISPEE344 pKa = 3.67SSSRR348 pKa = 11.84RR349 pKa = 11.84KK350 pKa = 8.54SARR353 pKa = 11.84RR354 pKa = 11.84RR355 pKa = 11.84SYY357 pKa = 10.49SRR359 pKa = 11.84SPATSRR365 pKa = 11.84SRR367 pKa = 11.84SVSPRR372 pKa = 11.84PKK374 pKa = 9.91DD375 pKa = 3.31SRR377 pKa = 11.84RR378 pKa = 11.84AKK380 pKa = 10.5DD381 pKa = 3.16KK382 pKa = 11.43SRR384 pKa = 11.84DD385 pKa = 3.28RR386 pKa = 11.84SHH388 pKa = 8.44AMDD391 pKa = 4.47RR392 pKa = 11.84PRR394 pKa = 11.84QRR396 pKa = 11.84DD397 pKa = 3.0RR398 pKa = 11.84SRR400 pKa = 11.84QRR402 pKa = 11.84DD403 pKa = 3.13RR404 pKa = 11.84SRR406 pKa = 11.84DD407 pKa = 3.19HH408 pKa = 6.1GRR410 pKa = 11.84NRR412 pKa = 11.84SRR414 pKa = 11.84LRR416 pKa = 11.84SRR418 pKa = 11.84DD419 pKa = 3.01RR420 pKa = 11.84DD421 pKa = 3.34RR422 pKa = 11.84SRR424 pKa = 11.84DD425 pKa = 3.45RR426 pKa = 11.84ASGRR430 pKa = 11.84DD431 pKa = 3.24RR432 pKa = 11.84YY433 pKa = 10.14RR434 pKa = 11.84EE435 pKa = 3.68RR436 pKa = 11.84DD437 pKa = 3.12RR438 pKa = 11.84DD439 pKa = 3.34MDD441 pKa = 3.8RR442 pKa = 11.84SKK444 pKa = 11.34DD445 pKa = 3.42RR446 pKa = 11.84SRR448 pKa = 11.84DD449 pKa = 3.07RR450 pKa = 11.84SRR452 pKa = 11.84DD453 pKa = 3.19RR454 pKa = 11.84RR455 pKa = 11.84VEE457 pKa = 3.68QSSRR461 pKa = 11.84NRR463 pKa = 11.84NRR465 pKa = 11.84TRR467 pKa = 11.84NSSTSRR473 pKa = 11.84RR474 pKa = 11.84VRR476 pKa = 11.84GDD478 pKa = 2.86SRR480 pKa = 11.84SRR482 pKa = 11.84SLAAGAKK489 pKa = 9.95AGGSFGDD496 pKa = 4.34LRR498 pKa = 11.84AILNAKK504 pKa = 9.65HH505 pKa = 6.87DD506 pKa = 3.85GDD508 pKa = 4.26KK509 pKa = 10.92RR510 pKa = 11.84SGSQRR515 pKa = 11.84TSQDD519 pKa = 2.8HH520 pKa = 5.79GVVSVSGEE528 pKa = 4.2HH529 pKa = 6.51EE530 pKa = 4.79DD531 pKa = 5.27KK532 pKa = 10.72PDD534 pKa = 3.34APIPSKK540 pKa = 11.18DD541 pKa = 4.38LDD543 pKa = 3.38TDD545 pKa = 3.71MQIDD549 pKa = 3.73SGDD552 pKa = 3.47YY553 pKa = 10.2SAA555 pKa = 5.77

Molecular weight: 60.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8610

0

8610

3887913

49

6976

451.6

50.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.991 ± 0.026

1.478 ± 0.012

5.671 ± 0.017

5.406 ± 0.024

3.941 ± 0.016

5.26 ± 0.024

2.694 ± 0.012

6.289 ± 0.018

5.727 ± 0.025

9.341 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.01

4.551 ± 0.016

4.919 ± 0.021

4.592 ± 0.02

4.726 ± 0.016

9.424 ± 0.036

6.456 ± 0.019

6.107 ± 0.021

1.055 ± 0.008

2.918 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski