Amino acid dipepetide frequency for Streptomyces cellostaticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.348AlaAla: 21.348 ± 0.133	1.164AlaCys: 1.164 ± 0.024	8.355AlaAsp: 8.355 ± 0.06	8.821AlaGlu: 8.821 ± 0.093	3.619AlaPhe: 3.619 ± 0.045	12.981AlaGly: 12.981 ± 0.091	3.12AlaHis: 3.12 ± 0.04	3.362AlaIle: 3.362 ± 0.034	2.863AlaLys: 2.863 ± 0.042	14.791AlaLeu: 14.791 ± 0.098	2.417AlaMet: 2.417 ± 0.032	1.898AlaAsn: 1.898 ± 0.03	7.035AlaPro: 7.035 ± 0.073	4.094AlaGln: 4.094 ± 0.044	10.274AlaArg: 10.274 ± 0.079	5.831AlaSer: 5.831 ± 0.052	6.925AlaThr: 6.925 ± 0.06	12.352AlaVal: 12.352 ± 0.081	1.925AlaTrp: 1.925 ± 0.026	2.859AlaTyr: 2.859 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.15CysAla: 1.15 ± 0.022	0.105CysCys: 0.105 ± 0.007	0.479CysAsp: 0.479 ± 0.014	0.409CysGlu: 0.409 ± 0.01	0.233CysPhe: 0.233 ± 0.01	0.974CysGly: 0.974 ± 0.02	0.212CysHis: 0.212 ± 0.009	0.175CysIle: 0.175 ± 0.009	0.135CysLys: 0.135 ± 0.006	0.828CysLeu: 0.828 ± 0.019	0.125CysMet: 0.125 ± 0.006	0.162CysAsn: 0.162 ± 0.008	0.493CysPro: 0.493 ± 0.014	0.178CysGln: 0.178 ± 0.008	0.636CysArg: 0.636 ± 0.015	0.475CysSer: 0.475 ± 0.015	0.561CysThr: 0.561 ± 0.015	0.685CysVal: 0.685 ± 0.016	0.138CysTrp: 0.138 ± 0.007	0.157CysTyr: 0.157 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.464AspAla: 7.464 ± 0.063	0.445AspCys: 0.445 ± 0.013	3.421AspAsp: 3.421 ± 0.039	3.635AspGlu: 3.635 ± 0.041	1.7AspPhe: 1.7 ± 0.024	6.316AspGly: 6.316 ± 0.057	1.51AspHis: 1.51 ± 0.029	1.872AspIle: 1.872 ± 0.031	1.196AspLys: 1.196 ± 0.026	6.29AspLeu: 6.29 ± 0.052	0.798AspMet: 0.798 ± 0.018	0.961AspAsn: 0.961 ± 0.022	4.463AspPro: 4.463 ± 0.041	1.582AspGln: 1.582 ± 0.027	4.768AspArg: 4.768 ± 0.052	2.575AspSer: 2.575 ± 0.029	3.237AspThr: 3.237 ± 0.039	4.656AspVal: 4.656 ± 0.05	1.072AspTrp: 1.072 ± 0.021	1.159AspTyr: 1.159 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.37GluAla: 7.37 ± 0.089	0.375GluCys: 0.375 ± 0.013	2.794GluAsp: 2.794 ± 0.032	3.576GluGlu: 3.576 ± 0.049	1.444GluPhe: 1.444 ± 0.024	4.189GluGly: 4.189 ± 0.042	1.504GluHis: 1.504 ± 0.024	2.176GluIle: 2.176 ± 0.036	1.406GluLys: 1.406 ± 0.026	6.761GluLeu: 6.761 ± 0.063	0.851GluMet: 0.851 ± 0.017	1.0GluAsn: 1.0 ± 0.021	3.352GluPro: 3.352 ± 0.034	2.333GluGln: 2.333 ± 0.035	5.432GluArg: 5.432 ± 0.06	2.415GluSer: 2.415 ± 0.036	2.832GluThr: 2.832 ± 0.034	4.374GluVal: 4.374 ± 0.048	0.721GluTrp: 0.721 ± 0.018	1.088GluTyr: 1.088 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.702PheAla: 3.702 ± 0.038	0.278PheCys: 0.278 ± 0.011	1.934PheAsp: 1.934 ± 0.029	1.423PheGlu: 1.423 ± 0.024	0.875PhePhe: 0.875 ± 0.022	3.03PheGly: 3.03 ± 0.043	0.622PheHis: 0.622 ± 0.014	0.682PheIle: 0.682 ± 0.017	0.535PheLys: 0.535 ± 0.014	2.64PheLeu: 2.64 ± 0.041	0.399PheMet: 0.399 ± 0.012	0.567PheAsn: 0.567 ± 0.014	1.415PhePro: 1.415 ± 0.023	0.681PheGln: 0.681 ± 0.015	1.88PheArg: 1.88 ± 0.026	1.537PheSer: 1.537 ± 0.027	2.015PheThr: 2.015 ± 0.026	2.223PheVal: 2.223 ± 0.031	0.422PheTrp: 0.422 ± 0.013	0.602PheTyr: 0.602 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	10.824GlyAla: 10.824 ± 0.08	0.866GlyCys: 0.866 ± 0.018	5.015GlyAsp: 5.015 ± 0.042	5.001GlyGlu: 5.001 ± 0.045	2.863GlyPhe: 2.863 ± 0.034	8.584GlyGly: 8.584 ± 0.099	2.529GlyHis: 2.529 ± 0.035	3.478GlyIle: 3.478 ± 0.036	2.495GlyLys: 2.495 ± 0.032	9.607GlyLeu: 9.607 ± 0.072	1.96GlyMet: 1.96 ± 0.028	1.763GlyAsn: 1.763 ± 0.033	5.073GlyPro: 5.073 ± 0.051	2.814GlyGln: 2.814 ± 0.033	7.723GlyArg: 7.723 ± 0.064	5.364GlySer: 5.364 ± 0.048	6.475GlyThr: 6.475 ± 0.061	7.416GlyVal: 7.416 ± 0.066	1.743GlyTrp: 1.743 ± 0.027	2.328GlyTyr: 2.328 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.874HisAla: 2.874 ± 0.034	0.228HisCys: 0.228 ± 0.009	1.37HisAsp: 1.37 ± 0.023	1.262HisGlu: 1.262 ± 0.025	0.689HisPhe: 0.689 ± 0.016	2.575HisGly: 2.575 ± 0.031	0.724HisHis: 0.724 ± 0.018	0.722HisIle: 0.722 ± 0.017	0.39HisLys: 0.39 ± 0.012	2.574HisLeu: 2.574 ± 0.032	0.332HisMet: 0.332 ± 0.011	0.37HisAsn: 0.37 ± 0.011	1.955HisPro: 1.955 ± 0.03	0.665HisGln: 0.665 ± 0.015	2.223HisArg: 2.223 ± 0.031	1.063HisSer: 1.063 ± 0.022	1.48HisThr: 1.48 ± 0.025	1.742HisVal: 1.742 ± 0.023	0.388HisTrp: 0.388 ± 0.011	0.507HisTyr: 0.507 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.722IleAla: 4.722 ± 0.049	0.285IleCys: 0.285 ± 0.011	2.165IleAsp: 2.165 ± 0.033	1.939IleGlu: 1.939 ± 0.03	0.661IlePhe: 0.661 ± 0.018	3.493IleGly: 3.493 ± 0.038	0.612IleHis: 0.612 ± 0.016	0.851IleIle: 0.851 ± 0.021	0.746IleLys: 0.746 ± 0.018	2.426IleLeu: 2.426 ± 0.032	0.418IleMet: 0.418 ± 0.013	0.691IleAsn: 0.691 ± 0.019	1.802IlePro: 1.802 ± 0.027	0.76IleGln: 0.76 ± 0.02	2.305IleArg: 2.305 ± 0.034	1.66IleSer: 1.66 ± 0.026	2.199IleThr: 2.199 ± 0.034	2.638IleVal: 2.638 ± 0.04	0.382IleTrp: 0.382 ± 0.012	0.533IleTyr: 0.533 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.057LysAla: 3.057 ± 0.042	0.126LysCys: 0.126 ± 0.008	1.413LysAsp: 1.413 ± 0.026	1.176LysGlu: 1.176 ± 0.022	0.46LysPhe: 0.46 ± 0.013	1.9LysGly: 1.9 ± 0.036	0.443LysHis: 0.443 ± 0.015	0.86LysIle: 0.86 ± 0.021	0.905LysLys: 0.905 ± 0.026	2.057LysLeu: 2.057 ± 0.029	0.388LysMet: 0.388 ± 0.013	0.553LysAsn: 0.553 ± 0.016	1.359LysPro: 1.359 ± 0.024	0.752LysGln: 0.752 ± 0.019	1.375LysArg: 1.375 ± 0.023	1.183LysSer: 1.183 ± 0.023	1.326LysThr: 1.326 ± 0.027	1.959LysVal: 1.959 ± 0.036	0.285LysTrp: 0.285 ± 0.01	0.5LysTyr: 0.5 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.504LeuAla: 15.504 ± 0.106	0.892LeuCys: 0.892 ± 0.018	6.708LeuAsp: 6.708 ± 0.056	4.755LeuGlu: 4.755 ± 0.049	2.647LeuPhe: 2.647 ± 0.034	9.367LeuGly: 9.367 ± 0.066	2.483LeuHis: 2.483 ± 0.032	3.288LeuIle: 3.288 ± 0.039	2.178LeuLys: 2.178 ± 0.033	11.483LeuLeu: 11.483 ± 0.085	1.613LeuMet: 1.613 ± 0.028	1.725LeuAsn: 1.725 ± 0.026	6.651LeuPro: 6.651 ± 0.052	2.289LeuGln: 2.289 ± 0.03	8.769LeuArg: 8.769 ± 0.068	5.38LeuSer: 5.38 ± 0.046	7.045LeuThr: 7.045 ± 0.057	8.8LeuVal: 8.8 ± 0.068	1.374LeuTrp: 1.374 ± 0.024	1.979LeuTyr: 1.979 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.212MetAla: 2.212 ± 0.03	0.139MetCys: 0.139 ± 0.007	0.856MetAsp: 0.856 ± 0.018	0.721MetGlu: 0.721 ± 0.017	0.43MetPhe: 0.43 ± 0.014	1.278MetGly: 1.278 ± 0.019	0.375MetHis: 0.375 ± 0.011	0.624MetIle: 0.624 ± 0.015	0.423MetLys: 0.423 ± 0.014	1.603MetLeu: 1.603 ± 0.024	0.279MetMet: 0.279 ± 0.012	0.422MetAsn: 0.422 ± 0.014	1.091MetPro: 1.091 ± 0.02	0.462MetGln: 0.462 ± 0.014	1.345MetArg: 1.345 ± 0.024	1.289MetSer: 1.289 ± 0.022	1.523MetThr: 1.523 ± 0.024	1.22MetVal: 1.22 ± 0.023	0.21MetTrp: 0.21 ± 0.009	0.327MetTyr: 0.327 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.206AsnAla: 2.206 ± 0.032	0.168AsnCys: 0.168 ± 0.008	0.98AsnAsp: 0.98 ± 0.021	0.77AsnGlu: 0.77 ± 0.019	0.487AsnPhe: 0.487 ± 0.013	1.912AsnGly: 1.912 ± 0.032	0.419AsnHis: 0.419 ± 0.013	0.644AsnIle: 0.644 ± 0.018	0.433AsnLys: 0.433 ± 0.014	1.654AsnLeu: 1.654 ± 0.026	0.292AsnMet: 0.292 ± 0.01	0.445AsnAsn: 0.445 ± 0.015	1.383AsnPro: 1.383 ± 0.027	0.54AsnGln: 0.54 ± 0.016	1.242AsnArg: 1.242 ± 0.021	0.962AsnSer: 0.962 ± 0.02	1.129AsnThr: 1.129 ± 0.022	1.343AsnVal: 1.343 ± 0.021	0.302AsnTrp: 0.302 ± 0.013	0.428AsnTyr: 0.428 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.734ProAla: 8.734 ± 0.076	0.36ProCys: 0.36 ± 0.012	4.494ProAsp: 4.494 ± 0.051	4.311ProGlu: 4.311 ± 0.04	1.538ProPhe: 1.538 ± 0.023	6.609ProGly: 6.609 ± 0.051	1.483ProHis: 1.483 ± 0.026	1.204ProIle: 1.204 ± 0.024	1.241ProLys: 1.241 ± 0.023	5.525ProLeu: 5.525 ± 0.05	0.963ProMet: 0.963 ± 0.019	0.885ProAsn: 0.885 ± 0.017	3.349ProPro: 3.349 ± 0.046	1.919ProGln: 1.919 ± 0.039	3.776ProArg: 3.776 ± 0.042	3.145ProSer: 3.145 ± 0.042	3.105ProThr: 3.105 ± 0.037	5.509ProVal: 5.509 ± 0.047	0.907ProTrp: 0.907 ± 0.02	1.491ProTyr: 1.491 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.042GlnAla: 4.042 ± 0.045	0.2GlnCys: 0.2 ± 0.008	1.5GlnAsp: 1.5 ± 0.023	1.525GlnGlu: 1.525 ± 0.026	0.696GlnPhe: 0.696 ± 0.018	2.461GlnGly: 2.461 ± 0.035	0.719GlnHis: 0.719 ± 0.016	1.079GlnIle: 1.079 ± 0.022	0.656GlnLys: 0.656 ± 0.016	3.203GlnLeu: 3.203 ± 0.037	0.495GlnMet: 0.495 ± 0.016	0.519GlnAsn: 0.519 ± 0.016	1.802GlnPro: 1.802 ± 0.031	1.352GlnGln: 1.352 ± 0.025	2.384GlnArg: 2.384 ± 0.029	1.328GlnSer: 1.328 ± 0.023	1.434GlnThr: 1.434 ± 0.023	2.503GlnVal: 2.503 ± 0.036	0.533GlnTrp: 0.533 ± 0.015	0.673GlnTyr: 0.673 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.814ArgAla: 9.814 ± 0.086	0.62ArgCys: 0.62 ± 0.016	4.083ArgAsp: 4.083 ± 0.043	4.634ArgGlu: 4.634 ± 0.052	2.311ArgPhe: 2.311 ± 0.029	5.527ArgGly: 5.527 ± 0.044	2.188ArgHis: 2.188 ± 0.033	3.344ArgIle: 3.344 ± 0.034	1.589ArgLys: 1.589 ± 0.028	9.119ArgLeu: 9.119 ± 0.075	1.67ArgMet: 1.67 ± 0.025	1.334ArgAsn: 1.334 ± 0.021	4.907ArgPro: 4.907 ± 0.05	2.426ArgGln: 2.426 ± 0.03	7.544ArgArg: 7.544 ± 0.075	3.969ArgSer: 3.969 ± 0.036	5.396ArgThr: 5.396 ± 0.051	5.696ArgVal: 5.696 ± 0.049	1.379ArgTrp: 1.379 ± 0.025	1.838ArgTyr: 1.838 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.806SerAla: 6.806 ± 0.062	0.444SerCys: 0.444 ± 0.013	2.638SerAsp: 2.638 ± 0.034	2.289SerGlu: 2.289 ± 0.033	1.536SerPhe: 1.536 ± 0.026	5.996SerGly: 5.996 ± 0.057	1.026SerHis: 1.026 ± 0.02	1.384SerIle: 1.384 ± 0.025	1.044SerLys: 1.044 ± 0.02	5.008SerLeu: 5.008 ± 0.048	1.047SerMet: 1.047 ± 0.02	0.897SerAsn: 0.897 ± 0.019	3.172SerPro: 3.172 ± 0.036	1.272SerGln: 1.272 ± 0.025	3.6SerArg: 3.6 ± 0.043	2.912SerSer: 2.912 ± 0.041	3.039SerThr: 3.039 ± 0.037	4.225SerVal: 4.225 ± 0.038	0.911SerTrp: 0.911 ± 0.018	1.299SerTyr: 1.299 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.069ThrAla: 9.069 ± 0.07	0.464ThrCys: 0.464 ± 0.014	3.655ThrAsp: 3.655 ± 0.042	3.238ThrGlu: 3.238 ± 0.036	1.619ThrPhe: 1.619 ± 0.026	6.945ThrGly: 6.945 ± 0.065	1.261ThrHis: 1.261 ± 0.021	1.643ThrIle: 1.643 ± 0.026	1.225ThrLys: 1.225 ± 0.022	5.869ThrLeu: 5.869 ± 0.051	0.888ThrMet: 0.888 ± 0.019	1.001ThrAsn: 1.001 ± 0.022	4.1ThrPro: 4.1 ± 0.043	1.371ThrGln: 1.371 ± 0.028	3.835ThrArg: 3.835 ± 0.037	3.146ThrSer: 3.146 ± 0.033	3.786ThrThr: 3.786 ± 0.044	6.093ThrVal: 6.093 ± 0.056	0.919ThrTrp: 0.919 ± 0.02	1.392ThrTyr: 1.392 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	10.514ValAla: 10.514 ± 0.074	0.801ValCys: 0.801 ± 0.018	4.787ValAsp: 4.787 ± 0.056	4.488ValGlu: 4.488 ± 0.052	2.477ValPhe: 2.477 ± 0.038	6.304ValGly: 6.304 ± 0.055	2.063ValHis: 2.063 ± 0.03	2.838ValIle: 2.838 ± 0.038	1.802ValLys: 1.802 ± 0.03	9.516ValLeu: 9.516 ± 0.069	1.374ValMet: 1.374 ± 0.024	1.669ValAsn: 1.669 ± 0.026	5.244ValPro: 5.244 ± 0.045	2.269ValGln: 2.269 ± 0.031	7.155ValArg: 7.155 ± 0.064	4.333ValSer: 4.333 ± 0.043	5.677ValThr: 5.677 ± 0.05	7.712ValVal: 7.712 ± 0.07	1.207ValTrp: 1.207 ± 0.023	1.627ValTyr: 1.627 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.723TrpAla: 1.723 ± 0.026	0.161TrpCys: 0.161 ± 0.007	0.847TrpAsp: 0.847 ± 0.019	0.72TrpGlu: 0.72 ± 0.016	0.514TrpPhe: 0.514 ± 0.015	1.071TrpGly: 1.071 ± 0.023	0.415TrpHis: 0.415 ± 0.013	0.574TrpIle: 0.574 ± 0.016	0.383TrpLys: 0.383 ± 0.014	1.84TrpLeu: 1.84 ± 0.032	0.277TrpMet: 0.277 ± 0.01	0.444TrpAsn: 0.444 ± 0.016	0.806TrpPro: 0.806 ± 0.019	0.682TrpGln: 0.682 ± 0.017	1.315TrpArg: 1.315 ± 0.025	0.978TrpSer: 0.978 ± 0.023	1.061TrpThr: 1.061 ± 0.02	0.998TrpVal: 0.998 ± 0.019	0.346TrpTrp: 0.346 ± 0.011	0.385TrpTyr: 0.385 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.9TyrAla: 2.9 ± 0.039	0.187TyrCys: 0.187 ± 0.008	1.622TyrAsp: 1.622 ± 0.026	1.3TyrGlu: 1.3 ± 0.021	0.663TyrPhe: 0.663 ± 0.018	2.407TyrGly: 2.407 ± 0.033	0.425TyrHis: 0.425 ± 0.012	0.487TyrIle: 0.487 ± 0.015	0.436TyrLys: 0.436 ± 0.015	2.138TyrLeu: 2.138 ± 0.027	0.243TyrMet: 0.243 ± 0.01	0.433TyrAsn: 0.433 ± 0.012	1.094TyrPro: 1.094 ± 0.021	0.664TyrGln: 0.664 ± 0.018	1.809TyrArg: 1.809 ± 0.028	0.998TyrSer: 0.998 ± 0.021	1.272TyrThr: 1.272 ± 0.023	1.714TyrVal: 1.714 ± 0.026	0.379TyrTrp: 0.379 ± 0.013	0.485TyrTyr: 0.485 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8025 proteins (2665306 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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