Salinisphaera shabanensis E1L3A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Salinisphaerales; Salinisphaeraceae; Salinisphaera; Salinisphaera shabanensis

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3524 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2G3S5|U2G3S5_9GAMM Histidine kinase OS=Salinisphaera shabanensis E1L3A OX=1033802 GN=SSPSH_000110 PE=4 SV=1
MM1 pKa = 7.72VDD3 pKa = 3.22RR4 pKa = 11.84DD5 pKa = 3.93YY6 pKa = 11.64FGTDD10 pKa = 2.78SEE12 pKa = 4.26AQEE15 pKa = 4.69LIGNAILQYY24 pKa = 11.45DD25 pKa = 3.89MSFDD29 pKa = 4.81HH30 pKa = 7.14IDD32 pKa = 3.29SSTLAGVEE40 pKa = 4.01YY41 pKa = 10.5RR42 pKa = 11.84DD43 pKa = 4.04ASSDD47 pKa = 3.5DD48 pKa = 3.26SSFYY52 pKa = 11.38GDD54 pKa = 4.31AAPIDD59 pKa = 4.12IANPDD64 pKa = 3.54YY65 pKa = 11.37SGAPSSLNVYY75 pKa = 8.72MGNDD79 pKa = 2.87QDD81 pKa = 5.04YY82 pKa = 7.24EE83 pKa = 4.5TKK85 pKa = 10.64SVFLTQNLSFYY96 pKa = 11.31DD97 pKa = 3.78RR98 pKa = 11.84FVVTAGVRR106 pKa = 11.84NDD108 pKa = 3.62SLDD111 pKa = 3.63LSSTDD116 pKa = 2.83ITGVTEE122 pKa = 4.25SDD124 pKa = 3.85DD125 pKa = 3.82FSEE128 pKa = 4.46TSVRR132 pKa = 11.84GALTYY137 pKa = 10.21IVNDD141 pKa = 4.19EE142 pKa = 3.77ISTYY146 pKa = 9.7ISQVEE151 pKa = 4.67SVAPPSIGVKK161 pKa = 9.17PEE163 pKa = 3.66RR164 pKa = 11.84GEE166 pKa = 3.95QYY168 pKa = 9.44TRR170 pKa = 11.84RR171 pKa = 11.84PAA173 pKa = 3.54

Molecular weight: 18.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2FWQ3|U2FWQ3_9GAMM 30S ribosomal protein S15 OS=Salinisphaera shabanensis E1L3A OX=1033802 GN=rpsO PE=3 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84STLRR6 pKa = 11.84HH7 pKa = 5.25AVAIALFICAPGVAGANPSDD27 pKa = 3.5QSGPARR33 pKa = 11.84VANAAGEE40 pKa = 4.18YY41 pKa = 10.55LVFSDD46 pKa = 6.03ALTADD51 pKa = 4.05CMLHH55 pKa = 6.49RR56 pKa = 11.84GRR58 pKa = 11.84SRR60 pKa = 11.84RR61 pKa = 11.84IANTHH66 pKa = 4.78PTRR69 pKa = 11.84AIRR72 pKa = 11.84VVLIAYY78 pKa = 7.24TGKK81 pKa = 8.2TRR83 pKa = 11.84AQGDD87 pKa = 4.02SEE89 pKa = 5.46FILDD93 pKa = 4.42PKK95 pKa = 10.2TEE97 pKa = 4.35PKK99 pKa = 10.39PLGCDD104 pKa = 3.07SVSGLARR111 pKa = 11.84RR112 pKa = 11.84WEE114 pKa = 4.03IVHH117 pKa = 6.59ARR119 pKa = 11.84FVSPARR125 pKa = 11.84RR126 pKa = 3.34

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3524

0

3524

1120061

27

3756

317.8

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.352 ± 0.055

0.942 ± 0.014

6.526 ± 0.035

5.76 ± 0.037

3.543 ± 0.028

8.1 ± 0.034

2.285 ± 0.024

4.99 ± 0.029

2.7 ± 0.032

10.081 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.021

2.857 ± 0.023

4.686 ± 0.026

3.5 ± 0.023

7.344 ± 0.042

5.383 ± 0.028

5.253 ± 0.027

7.322 ± 0.032

1.365 ± 0.017

2.595 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski