Lake Sarah-associated circular molecule 5

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQI4|A0A140AQI4_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 5 OX=1685730 PE=3 SV=1
MM1 pKa = 7.47SARR4 pKa = 11.84NFTFTQNNYY13 pKa = 10.35GDD15 pKa = 3.96TEE17 pKa = 4.28LVDD20 pKa = 4.99GVQCRR25 pKa = 11.84YY26 pKa = 9.8IIYY29 pKa = 9.29GKK31 pKa = 10.18EE32 pKa = 3.85VGEE35 pKa = 4.2SDD37 pKa = 4.58LLQLQGVICFNEE49 pKa = 4.51KK50 pKa = 9.63IRR52 pKa = 11.84EE53 pKa = 4.03SAVRR57 pKa = 11.84KK58 pKa = 9.64KK59 pKa = 11.06LPGCHH64 pKa = 6.33IEE66 pKa = 3.96IAKK69 pKa = 9.46VLPAAIEE76 pKa = 4.16YY77 pKa = 9.53CKK79 pKa = 10.53KK80 pKa = 10.84DD81 pKa = 3.23GDD83 pKa = 3.52WTEE86 pKa = 5.1RR87 pKa = 11.84GDD89 pKa = 4.49SPMTPKK95 pKa = 10.67EE96 pKa = 3.71KK97 pKa = 10.64GALEE101 pKa = 3.85KK102 pKa = 10.89RR103 pKa = 11.84KK104 pKa = 9.12WDD106 pKa = 4.33EE107 pKa = 3.49IRR109 pKa = 11.84VACEE113 pKa = 3.22EE114 pKa = 4.21GRR116 pKa = 11.84FEE118 pKa = 6.01DD119 pKa = 4.33LPDD122 pKa = 5.2DD123 pKa = 3.61IRR125 pKa = 11.84YY126 pKa = 10.14KK127 pKa = 10.66NLRR130 pKa = 11.84LNKK133 pKa = 8.04MHH135 pKa = 7.06RR136 pKa = 11.84CEE138 pKa = 3.99ALRR141 pKa = 11.84SRR143 pKa = 11.84EE144 pKa = 4.17LEE146 pKa = 4.16DD147 pKa = 5.88SEE149 pKa = 5.68AQHH152 pKa = 5.63LWYY155 pKa = 9.46WGEE158 pKa = 3.89AGTGKK163 pKa = 9.92SRR165 pKa = 11.84KK166 pKa = 9.4ARR168 pKa = 11.84EE169 pKa = 3.99DD170 pKa = 3.11HH171 pKa = 6.15PRR173 pKa = 11.84AYY175 pKa = 11.07LKK177 pKa = 9.78MCNKK181 pKa = 8.08WWCGYY186 pKa = 7.39TEE188 pKa = 4.41EE189 pKa = 4.32EE190 pKa = 4.4TVLIEE195 pKa = 5.8DD196 pKa = 4.16FDD198 pKa = 4.24KK199 pKa = 11.35KK200 pKa = 10.35HH201 pKa = 6.75DD202 pKa = 3.95VLGHH206 pKa = 5.95HH207 pKa = 6.69LKK209 pKa = 9.96IWGDD213 pKa = 3.88RR214 pKa = 11.84YY215 pKa = 10.56PFLAEE220 pKa = 4.42LKK222 pKa = 10.04GDD224 pKa = 3.78TMKK227 pKa = 10.3IRR229 pKa = 11.84PKK231 pKa = 10.58QIIVTSNYY239 pKa = 8.94HH240 pKa = 6.75PSAIWFDD247 pKa = 4.09EE248 pKa = 4.17EE249 pKa = 4.1TLLPILRR256 pKa = 11.84RR257 pKa = 11.84FKK259 pKa = 10.81CVEE262 pKa = 4.04FKK264 pKa = 10.91KK265 pKa = 11.04LEE267 pKa = 4.15ASTT270 pKa = 3.88

Molecular weight: 31.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQI4|A0A140AQI4_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 5 OX=1685730 PE=3 SV=1
MM1 pKa = 7.47SARR4 pKa = 11.84NFTFTQNNYY13 pKa = 10.35GDD15 pKa = 3.96TEE17 pKa = 4.28LVDD20 pKa = 4.99GVQCRR25 pKa = 11.84YY26 pKa = 9.8IIYY29 pKa = 9.29GKK31 pKa = 10.18EE32 pKa = 3.85VGEE35 pKa = 4.2SDD37 pKa = 4.58LLQLQGVICFNEE49 pKa = 4.51KK50 pKa = 9.63IRR52 pKa = 11.84EE53 pKa = 4.03SAVRR57 pKa = 11.84KK58 pKa = 9.64KK59 pKa = 11.06LPGCHH64 pKa = 6.33IEE66 pKa = 3.96IAKK69 pKa = 9.46VLPAAIEE76 pKa = 4.16YY77 pKa = 9.53CKK79 pKa = 10.53KK80 pKa = 10.84DD81 pKa = 3.23GDD83 pKa = 3.52WTEE86 pKa = 5.1RR87 pKa = 11.84GDD89 pKa = 4.49SPMTPKK95 pKa = 10.67EE96 pKa = 3.71KK97 pKa = 10.64GALEE101 pKa = 3.85KK102 pKa = 10.89RR103 pKa = 11.84KK104 pKa = 9.12WDD106 pKa = 4.33EE107 pKa = 3.49IRR109 pKa = 11.84VACEE113 pKa = 3.22EE114 pKa = 4.21GRR116 pKa = 11.84FEE118 pKa = 6.01DD119 pKa = 4.33LPDD122 pKa = 5.2DD123 pKa = 3.61IRR125 pKa = 11.84YY126 pKa = 10.14KK127 pKa = 10.66NLRR130 pKa = 11.84LNKK133 pKa = 8.04MHH135 pKa = 7.06RR136 pKa = 11.84CEE138 pKa = 3.99ALRR141 pKa = 11.84SRR143 pKa = 11.84EE144 pKa = 4.17LEE146 pKa = 4.16DD147 pKa = 5.88SEE149 pKa = 5.68AQHH152 pKa = 5.63LWYY155 pKa = 9.46WGEE158 pKa = 3.89AGTGKK163 pKa = 9.92SRR165 pKa = 11.84KK166 pKa = 9.4ARR168 pKa = 11.84EE169 pKa = 3.99DD170 pKa = 3.11HH171 pKa = 6.15PRR173 pKa = 11.84AYY175 pKa = 11.07LKK177 pKa = 9.78MCNKK181 pKa = 8.08WWCGYY186 pKa = 7.39TEE188 pKa = 4.41EE189 pKa = 4.32EE190 pKa = 4.4TVLIEE195 pKa = 5.8DD196 pKa = 4.16FDD198 pKa = 4.24KK199 pKa = 11.35KK200 pKa = 10.35HH201 pKa = 6.75DD202 pKa = 3.95VLGHH206 pKa = 5.95HH207 pKa = 6.69LKK209 pKa = 9.96IWGDD213 pKa = 3.88RR214 pKa = 11.84YY215 pKa = 10.56PFLAEE220 pKa = 4.42LKK222 pKa = 10.04GDD224 pKa = 3.78TMKK227 pKa = 10.3IRR229 pKa = 11.84PKK231 pKa = 10.58QIIVTSNYY239 pKa = 8.94HH240 pKa = 6.75PSAIWFDD247 pKa = 4.09EE248 pKa = 4.17EE249 pKa = 4.1TLLPILRR256 pKa = 11.84RR257 pKa = 11.84FKK259 pKa = 10.81CVEE262 pKa = 4.04FKK264 pKa = 10.91KK265 pKa = 11.04LEE267 pKa = 4.15ASTT270 pKa = 3.88

Molecular weight: 31.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

270

270

270

270.0

31.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.556 ± 0.0

3.333 ± 0.0

6.667 ± 0.0

10.741 ± 0.0

3.333 ± 0.0

6.296 ± 0.0

2.963 ± 0.0

5.926 ± 0.0

9.63 ± 0.0

8.519 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.852 ± 0.0

2.963 ± 0.0

3.704 ± 0.0

2.222 ± 0.0

7.407 ± 0.0

3.704 ± 0.0

4.444 ± 0.0

4.074 ± 0.0

2.963 ± 0.0

3.704 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski