Streptococcus satellite phage Javan461

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZP13|A0A4D5ZP13_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan461 OX=2558712 GN=JavanS461_0008 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.67TPKK9 pKa = 10.21TFTFHH14 pKa = 5.7YY15 pKa = 8.55TFKK18 pKa = 11.1DD19 pKa = 3.09FDD21 pKa = 4.09TAQVACHH28 pKa = 6.88AILGYY33 pKa = 7.77MTGTYY38 pKa = 7.11EE39 pKa = 4.76QPVIDD44 pKa = 3.97ATYY47 pKa = 10.82HH48 pKa = 6.24NDD50 pKa = 3.59DD51 pKa = 3.72QGGHH55 pKa = 6.63ANQLVLKK62 pKa = 9.62YY63 pKa = 10.97VEE65 pKa = 4.33DD66 pKa = 3.91RR67 pKa = 11.84KK68 pKa = 10.54LSKK71 pKa = 10.3VFKK74 pKa = 10.15RR75 pKa = 11.84ICDD78 pKa = 3.69SFKK81 pKa = 10.93DD82 pKa = 4.21YY83 pKa = 11.52YY84 pKa = 10.51NQPEE88 pKa = 4.31DD89 pKa = 3.62MTDD92 pKa = 3.15EE93 pKa = 4.12EE94 pKa = 5.06LDD96 pKa = 3.68YY97 pKa = 11.37QRR99 pKa = 11.84VVSLSKK105 pKa = 9.75STQGKK110 pKa = 8.82VNNRR114 pKa = 11.84DD115 pKa = 3.17TLIAFISDD123 pKa = 3.38HH124 pKa = 5.93NQLAEE129 pKa = 4.25HH130 pKa = 6.91LSMNYY135 pKa = 10.19KK136 pKa = 10.52EE137 pKa = 4.5MTPEE141 pKa = 3.92DD142 pKa = 3.6LGAILEE148 pKa = 4.71SISQAFNHH156 pKa = 6.73LYY158 pKa = 11.22DD159 pKa = 3.83MVVEE163 pKa = 4.32GQLLVKK169 pKa = 10.69

Molecular weight: 19.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNR0|A0A4D5ZNR0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan461 OX=2558712 GN=JavanS461_0012 PE=4 SV=1
MM1 pKa = 7.57SGGGAYY7 pKa = 9.43VCKK10 pKa = 10.2SQKK13 pKa = 9.01PKK15 pKa = 9.91EE16 pKa = 4.06RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84QGLSVYY25 pKa = 7.65EE26 pKa = 4.27TLTLMIAFGTLIVAIMNNKK45 pKa = 8.89NKK47 pKa = 10.56

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3203

46

562

145.6

16.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.369 ± 0.354

0.687 ± 0.264

5.151 ± 0.514

7.555 ± 0.584

3.934 ± 0.352

4.902 ± 0.39

1.998 ± 0.325

7.368 ± 0.385

9.21 ± 0.547

10.272 ± 0.499

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.31 ± 0.303

5.089 ± 0.289

3.341 ± 0.506

4.621 ± 0.236

5.526 ± 0.352

5.214 ± 0.275

6.119 ± 0.4

4.683 ± 0.363

0.874 ± 0.163

4.777 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski